Click here to switch to the map view.

The map label for this gene is pckA [H]

Identifier: 157372849

GI number: 157372849

Start: 5092919

End: 5094538

Strand: Direct

Name: pckA [H]

Synonym: Spro_4617

Alternate gene names: 157372849

Gene position: 5092919-5094538 (Clockwise)

Preceding gene: 157372848

Following gene: 157372850

Centisome position: 93.47

GC content: 55.99

Gene sequence:

>1620_bases
ATGCGTGTTAAAGGTATAACCCCTCAGGATCTGGCCGCTTATGGGATTCATAACGTCGGCGAAATCGTTCACAACCCGAG
TTATGACTTGCTGTTCAAGGAAGAAACAGACCCATCTCTGGAAGGTTTTGAGCGTGGGGTGGTAACCAAACTGGGTGCTG
TATCCGTCGACACCGGTATTTTTACCGGCCGTTCCCCGAAAGATAAGTACATTGTCCGTGACGACATCACCCGCGACACC
GTGTGGTGGGCCGATCAGGGTAAAGGTAAAAACGACAACAAACCCCTCAGCCCGGAAACCTGGGCAGACCTGAAACAGCT
GGTCACCACACAACTCTCCGGCAAACGTCTGTTTGTGATGGATACCTTCTGCGGCGCCAACGCCGACTCCCGTCTGAAAG
TTCGCTTTATTACCGAGGTAGCCTGGCAGGCGCACTTTGTTAAAAACATGTTCATCCGTCCGACGGATGAAGAGCTGCAG
GATTTCGAGCCAGACTTCGTGGTCATGAACGGTGCCAAATGCACTAACCCGAACTGGCAGCAACAGGGCCTGAACTCGGA
AAACTTTGTCGCCTTCAACCTGACCGAGCGCATGCAACTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAAAAGGGTA
TGTTCTCCATCATGAACTACCTGTTGCCGCTGAAAGGCATCGCCTCAATGCACTGCTCGGCCAACGTAGGCGAGAAAGGC
GACGTGGCGGTATTCTTCGGCCTGTCCGGCACCGGCAAAACCACCCTGTCCACCGATCCGAAACGCCAACTGATTGGCGA
TGACGAGCACGGTTGGGATGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCCAAAACCATCAAGCTGTCTGAAG
AAGCCGAGCCGGATATCTATCACGCCATCAAACGCGATGCGCTGCTGGAAAACGTCGTCGTTCTGGCTGACGGCACCATA
GATTTCAATGATGGTTCGAAAACCGAGAATACCCGCGTTTCCTATCCGATCTATCACATCCAGAATATCGTGAAGCCGGT
TTCCAAAGCGGGTCATGCCACTAAGGTGATCTTCCTGACCGCAGACGCCTTCGGCGTACTGCCGCCGGTATCTCGCCTGA
CCGCTAACCAGACCCAGTACCACTTCCTGTCTGGCTTTACCGCCAAGCTGGCCGGTACCGAGCGCGGCGTGACCGAGCCG
ACCCCAACCTTCTCCGCCTGCTTCGGCGCAGCCTTCCTGTCGCTGCACCCAACGCAGTACGCCGAAGTGCTGGTGAAACG
CATGCAGGCCGCCGGCGCGCAGGCCTATCTGGTGAACACCGGCTGGAACGGCACCGGCAAACGTATCTCGATCAAGGATA
CCCGCGGCATTATCGACGCGATCCTCAGCGGTGAAATCGATAAGGCAGAAACCATTACTCTGCCTATCTTCGACCTGGCG
ATGCCAACCTCACTGCCGGGCGTTAACCCGGATATCCTGGATCCACGCGCCACTTACGCCAGCCTTGAGCAATGGCAAGA
GAAGGCACAGGATCTGGCCGAGCGTTTTGTCACCAACTTTGACAAATACACCGATACCCCGGCAGGTGCCGCGCTGGTCA
GCGCCGGCCCAAAACTGTAA

Upstream 100 bases:

>100_bases
AAAACCGACTAAAACGTTAAATATTTGATACCTATCGCGGTTACTCGCTGCTGAATCCCTACAATTGGCGCCAGAAATTC
TGTGACCAAGGAGTAGTGAC

Downstream 100 bases:

>100_bases
AACCTTCTTTTTACAGCTTCATCTAAGGCGTGGATCCCACGCCTTTTTTTATTTTTATCGGTTGTTATTTAATCAACACA
AAGGGATAATAAGCACTGCA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MRVKGITPQDLAAYGIHNVGEIVHNPSYDLLFKEETDPSLEGFERGVVTKLGAVSVDTGIFTGRSPKDKYIVRDDITRDT
VWWADQGKGKNDNKPLSPETWADLKQLVTTQLSGKRLFVMDTFCGANADSRLKVRFITEVAWQAHFVKNMFIRPTDEELQ
DFEPDFVVMNGAKCTNPNWQQQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEKG
DVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGVFNFEGGCYAKTIKLSEEAEPDIYHAIKRDALLENVVVLADGTI
DFNDGSKTENTRVSYPIYHIQNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTANQTQYHFLSGFTAKLAGTERGVTEP
TPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRISIKDTRGIIDAILSGEIDKAETITLPIFDLA
MPTSLPGVNPDILDPRATYASLEQWQEKAQDLAERFVTNFDKYTDTPAGAALVSAGPKL

Sequences:

>Translated_539_residues
MRVKGITPQDLAAYGIHNVGEIVHNPSYDLLFKEETDPSLEGFERGVVTKLGAVSVDTGIFTGRSPKDKYIVRDDITRDT
VWWADQGKGKNDNKPLSPETWADLKQLVTTQLSGKRLFVMDTFCGANADSRLKVRFITEVAWQAHFVKNMFIRPTDEELQ
DFEPDFVVMNGAKCTNPNWQQQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEKG
DVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGVFNFEGGCYAKTIKLSEEAEPDIYHAIKRDALLENVVVLADGTI
DFNDGSKTENTRVSYPIYHIQNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTANQTQYHFLSGFTAKLAGTERGVTEP
TPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRISIKDTRGIIDAILSGEIDKAETITLPIFDLA
MPTSLPGVNPDILDPRATYASLEQWQEKAQDLAERFVTNFDKYTDTPAGAALVSAGPKL
>Mature_539_residues
MRVKGITPQDLAAYGIHNVGEIVHNPSYDLLFKEETDPSLEGFERGVVTKLGAVSVDTGIFTGRSPKDKYIVRDDITRDT
VWWADQGKGKNDNKPLSPETWADLKQLVTTQLSGKRLFVMDTFCGANADSRLKVRFITEVAWQAHFVKNMFIRPTDEELQ
DFEPDFVVMNGAKCTNPNWQQQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEKG
DVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGVFNFEGGCYAKTIKLSEEAEPDIYHAIKRDALLENVVVLADGTI
DFNDGSKTENTRVSYPIYHIQNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTANQTQYHFLSGFTAKLAGTERGVTEP
TPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRISIKDTRGIIDAILSGEIDKAETITLPIFDLA
MPTSLPGVNPDILDPRATYASLEQWQEKAQDLAERFVTNFDKYTDTPAGAALVSAGPKL

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=540, Percent_Identity=86.4814814814815, Blast_Score=979, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=506, Percent_Identity=45.8498023715415, Blast_Score=441, Evalue=1e-124,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59264; Mature: 59264

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKGITPQDLAAYGIHNVGEIVHNPSYDLLFKEETDPSLEGFERGVVTKLGAVSVDTGI
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEECCCE
FTGRSPKDKYIVRDDITRDTVWWADQGKGKNDNKPLSPETWADLKQLVTTQLSGKRLFVM
ECCCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
DTFCGANADSRLKVRFITEVAWQAHFVKNMFIRPTDEELQDFEPDFVVMNGAKCTNPNWQ
EECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCCCCCHH
QQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEKG
HHCCCCCCEEEEEHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGVFNFEGGCYAKTIKLSEEAEPDIY
CEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHH
HAIKRDALLENVVVLADGTIDFNDGSKTENTRVSYPIYHIQNIVKPVSKAGHATKVIFLT
HHHHHHHHHCCEEEEECCEEECCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCEEEEEEE
ADAFGVLPPVSRLTANQTQYHFLSGFTAKLAGTERGVTEPTPTFSACFGAAFLSLHPTQY
CCCCCCCCCHHHHHCCCCHHHHHCCCHHEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
AEVLVKRMQAAGAQAYLVNTGWNGTGKRISIKDTRGIIDAILSGEIDKAETITLPIFDLA
HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEEEHHHHH
MPTSLPGVNPDILDPRATYASLEQWQEKAQDLAERFVTNFDKYTDTPAGAALVSAGPKL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCC
>Mature Secondary Structure
MRVKGITPQDLAAYGIHNVGEIVHNPSYDLLFKEETDPSLEGFERGVVTKLGAVSVDTGI
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEECCCE
FTGRSPKDKYIVRDDITRDTVWWADQGKGKNDNKPLSPETWADLKQLVTTQLSGKRLFVM
ECCCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
DTFCGANADSRLKVRFITEVAWQAHFVKNMFIRPTDEELQDFEPDFVVMNGAKCTNPNWQ
EECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCCCCCHH
QQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEKG
HHCCCCCCEEEEEHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGVFNFEGGCYAKTIKLSEEAEPDIY
CEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHH
HAIKRDALLENVVVLADGTIDFNDGSKTENTRVSYPIYHIQNIVKPVSKAGHATKVIFLT
HHHHHHHHHCCEEEEECCEEECCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCEEEEEEE
ADAFGVLPPVSRLTANQTQYHFLSGFTAKLAGTERGVTEPTPTFSACFGAAFLSLHPTQY
CCCCCCCCCHHHHHCCCCHHHHHCCCHHEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
AEVLVKRMQAAGAQAYLVNTGWNGTGKRISIKDTRGIIDAILSGEIDKAETITLPIFDLA
HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEEEHHHHH
MPTSLPGVNPDILDPRATYASLEQWQEKAQDLAERFVTNFDKYTDTPAGAALVSAGPKL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA