Click here to switch to the map view.

The map label for this gene is ascG [H]

Identifier: 157372824

GI number: 157372824

Start: 5065310

End: 5066293

Strand: Direct

Name: ascG [H]

Synonym: Spro_4592

Alternate gene names: 157372824

Gene position: 5065310-5066293 (Clockwise)

Preceding gene: 157372823

Following gene: 157372825

Centisome position: 92.96

GC content: 60.98

Gene sequence:

>984_bases
ATGATGGTGACACTGGAAGATGTCGCAGCCCTGGCGGGCGTGTCCCGCGCCACCGTGTCACGCGTAGTGAATGGCGATAC
CAACGTTAAGGGGCAAACCCGCGAGAAGGTTGAAAAGGCGGTGGCCCAACTTGGCTACACGCCCAACCCGGCCGCCCGTG
CGCTGGCCTCCAGCCACAGCAATACCCTGGGGTTGGTCACTACCTCCTATCGCGGCGGTTTCTTCGGCGCACTGATGGAC
TTTGTGCAAACCGAAGCCGAATCCCACGGCAAGCAATTGCTGGTGACCCAGGGGCGTAACAGTGCGGAAAACGAATGGCA
GGCGATCCAGCGGCTGTTCAATCTGCGCTGCGATGGCGTGATCCTGCACGTCCGTTTCCTCAGCGATGACCAATTGCGTC
AACTGGCCGCGGAGCAACGCCGCTTTGTGCTGCTCGATCGCCTGGTGCCCGGCCTGGAAGCGCACTGCGTCACTTTCGAT
CACCAGTACGCCAGCCGCATGGCAACACAACAATTGATTGATGCCGGCCACCGCCACATCGCCTGTATCAGCGGCCCGCG
CGACCGCCCTTCCAGTCTCTTGCGGCTGCAGGGTTTTGAACAGGCCATGCAGGCAGCGCATATCAAGCCGGTCGCCTGCG
CCGAAGGGCTTTACGATCTGGAGAGCGGTTATCGCTGCGCCGATCAACTGCTGAAAATGAAAACCCTGCCCACCGCCATC
TATTGCTGCAATGAAGAAATGGCTATCGGCGCACTGCTGGCCGTCAACGAACACCGCCTGCGGGTGCCGCAAGATATCTC
GCTGATCTGTTACGACAGCGGCGAACGCGCCCCCTTTGTCCGCCCGGCGCTGACCAGCGTGCATTTCCCGATTAGCGAAA
TGGCGCAATATGCCGCACGCCTGCTGATTGACCCGGCCACGCCACCGGTTAGCTTTGCGCCGATAGTCATCAGCCGCGAC
TCGATCGTGACGGCGAGCAAATAG

Upstream 100 bases:

>100_bases
TTAAATAACCGTCGGGCCAACCGCGGTTGCTGATACATTGCTATACCTAGTATACAGGGGTCGCTGAGTCGGCCCCATTT
TTGTCGTAATCAGGAGTCGG

Downstream 100 bases:

>100_bases
ATCACAAAAATATTTCAGAACTACTCATTGATTGCATCAAAAAGTAGATAGGCATCACAAAAACTCCCGTTGTCTGTGCT
AGGATCCGCTCCCTGGTGGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MMVTLEDVAALAGVSRATVSRVVNGDTNVKGQTREKVEKAVAQLGYTPNPAARALASSHSNTLGLVTTSYRGGFFGALMD
FVQTEAESHGKQLLVTQGRNSAENEWQAIQRLFNLRCDGVILHVRFLSDDQLRQLAAEQRRFVLLDRLVPGLEAHCVTFD
HQYASRMATQQLIDAGHRHIACISGPRDRPSSLLRLQGFEQAMQAAHIKPVACAEGLYDLESGYRCADQLLKMKTLPTAI
YCCNEEMAIGALLAVNEHRLRVPQDISLICYDSGERAPFVRPALTSVHFPISEMAQYAARLLIDPATPPVSFAPIVISRD
SIVTASK

Sequences:

>Translated_327_residues
MMVTLEDVAALAGVSRATVSRVVNGDTNVKGQTREKVEKAVAQLGYTPNPAARALASSHSNTLGLVTTSYRGGFFGALMD
FVQTEAESHGKQLLVTQGRNSAENEWQAIQRLFNLRCDGVILHVRFLSDDQLRQLAAEQRRFVLLDRLVPGLEAHCVTFD
HQYASRMATQQLIDAGHRHIACISGPRDRPSSLLRLQGFEQAMQAAHIKPVACAEGLYDLESGYRCADQLLKMKTLPTAI
YCCNEEMAIGALLAVNEHRLRVPQDISLICYDSGERAPFVRPALTSVHFPISEMAQYAARLLIDPATPPVSFAPIVISRD
SIVTASK
>Mature_327_residues
MMVTLEDVAALAGVSRATVSRVVNGDTNVKGQTREKVEKAVAQLGYTPNPAARALASSHSNTLGLVTTSYRGGFFGALMD
FVQTEAESHGKQLLVTQGRNSAENEWQAIQRLFNLRCDGVILHVRFLSDDQLRQLAAEQRRFVLLDRLVPGLEAHCVTFD
HQYASRMATQQLIDAGHRHIACISGPRDRPSSLLRLQGFEQAMQAAHIKPVACAEGLYDLESGYRCADQLLKMKTLPTAI
YCCNEEMAIGALLAVNEHRLRVPQDISLICYDSGERAPFVRPALTSVHFPISEMAQYAARLLIDPATPPVSFAPIVISRD
SIVTASK

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=338, Percent_Identity=38.7573964497041, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=36.5558912386707, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1789202, Length=335, Percent_Identity=35.5223880597015, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787948, Length=309, Percent_Identity=34.3042071197411, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=31.8318318318318, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1790369, Length=306, Percent_Identity=32.3529411764706, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1786540, Length=286, Percent_Identity=33.9160839160839, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1787580, Length=311, Percent_Identity=28.6173633440514, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI48994940, Length=304, Percent_Identity=27.6315789473684, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1790715, Length=303, Percent_Identity=25.7425742574257, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790689, Length=306, Percent_Identity=26.4705882352941, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787906, Length=257, Percent_Identity=28.0155642023346, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786268, Length=283, Percent_Identity=22.9681978798587, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35887; Mature: 35887

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVTLEDVAALAGVSRATVSRVVNGDTNVKGQTREKVEKAVAQLGYTPNPAARALASSHS
CEEEHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCC
NTLGLVTTSYRGGFFGALMDFVQTEAESHGKQLLVTQGRNSAENEWQAIQRLFNLRCDGV
CEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCE
ILHVRFLSDDQLRQLAAEQRRFVLLDRLVPGLEAHCVTFDHQYASRMATQQLIDAGHRHI
EEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCCEE
ACISGPRDRPSSLLRLQGFEQAMQAAHIKPVACAEGLYDLESGYRCADQLLKMKTLPTAI
EEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEE
YCCNEEMAIGALLAVNEHRLRVPQDISLICYDSGERAPFVRPALTSVHFPISEMAQYAAR
EECCCCHHHHEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHH
LLIDPATPPVSFAPIVISRDSIVTASK
HEECCCCCCCCEEEEEEECCCEEECCC
>Mature Secondary Structure
MMVTLEDVAALAGVSRATVSRVVNGDTNVKGQTREKVEKAVAQLGYTPNPAARALASSHS
CEEEHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCC
NTLGLVTTSYRGGFFGALMDFVQTEAESHGKQLLVTQGRNSAENEWQAIQRLFNLRCDGV
CEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCE
ILHVRFLSDDQLRQLAAEQRRFVLLDRLVPGLEAHCVTFDHQYASRMATQQLIDAGHRHI
EEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCCEE
ACISGPRDRPSSLLRLQGFEQAMQAAHIKPVACAEGLYDLESGYRCADQLLKMKTLPTAI
EEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEE
YCCNEEMAIGALLAVNEHRLRVPQDISLICYDSGERAPFVRPALTSVHFPISEMAQYAAR
EECCCCHHHHEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHH
LLIDPATPPVSFAPIVISRDSIVTASK
HEECCCCCCCCEEEEEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]