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The map label for this gene is yafC [C]

Identifier: 157372399

GI number: 157372399

Start: 4620781

End: 4621713

Strand: Reverse

Name: yafC [C]

Synonym: Spro_4166

Alternate gene names: 157372399

Gene position: 4621713-4620781 (Counterclockwise)

Preceding gene: 157372401

Following gene: 157372398

Centisome position: 84.82

GC content: 49.09

Gene sequence:

>933_bases
ATGCGTACCGATCTTAAAGGTATCCCTGTTTTCGTCGCTGTTGTTGAGTGCGGAAATTTTGCGCAAGCAGCCCAGGATTT
GAATATAACGCGCTCTGCCGTGGGTAAAGCCATAGCACGTCTCGAAGAGCGACTCGGCGTAACGTTATTCCAACGAACTA
CCCGCAACCAGACTCTGACTGAAGAAGGCGAGATTTTATATCAGCAATCACGTATTGCTTTGGACTGCATTCGTGAGGCT
GAAGATGTGATACATCTGGGTAAAACACAGGTAAAGGGTCGTCTCAGGATCAGCCTGCCGGTATTGTTAGGCCAGCGCTG
CATTGCACCAATCCTGCTCACTATTGCCAAGAGTTACCCACAACTAAAGCTCGAACTGTCTTTTAACGACCGGCAGGTAA
ATCTGTTTGACGAGGGGTTCGATTTGGCTGTTCGCATAGGCGAACTGGCTGACTCAAGCTTTTTGCAGGCCAGACGATTA
GGTCAGCACGGAATGATTCTATGTGCTTCCGCCCAGTATCTGAAAACTCACCTAGTGCCACGCACGTTATCAGAGCTACA
AGAACATCCAGCCATTGGATATCAGCTTGGGGGTAAGATTCTAAACTGGCAGCTCAACGACATGGAGGGAAGGGAGATCA
ATTTTCGACCTAATACGGTCCTCGCGGCGGATGATTTTGTCGCCATTGCAGCAGCAGTCATCAGTGATCTAGGAGTGGCA
TGGCTTCCGGACTGGCTGGTGTCAAAAGAAATCGCGCAGGGTCAAATTCAACAAATTTTGCCAGAATGCACGAGTATGAA
GTTCGCCATCAGTGCCGTCTGGTTGGAGGCCGCATGGATGCCACAAAAAATCCGCATCGTTATCGATGAGCTTCTGGCTA
AACTTCCCGAACAGATTTTCTATATTCCAGCTTGCGGAACAAGAGCGGAATGA

Upstream 100 bases:

>100_bases
TATTCTCTGGTTAACATTCATTACCCATAGAATATCACTATGTTTCTTTCTCCATTAGTGACATTGTGTATAGAGAATGT
ATACATAATGGAACCAATTG

Downstream 100 bases:

>100_bases
CCTAAGGTCGGTGGAAACTTCAACGGGTGAAATTTTCCAAATTCAATAAAGACTTCTTTGCTGCCTTTCTGAGCTACCCA
TAGCACCCTGTGCGGATTCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRTDLKGIPVFVAVVECGNFAQAAQDLNITRSAVGKAIARLEERLGVTLFQRTTRNQTLTEEGEILYQQSRIALDCIREA
EDVIHLGKTQVKGRLRISLPVLLGQRCIAPILLTIAKSYPQLKLELSFNDRQVNLFDEGFDLAVRIGELADSSFLQARRL
GQHGMILCASAQYLKTHLVPRTLSELQEHPAIGYQLGGKILNWQLNDMEGREINFRPNTVLAADDFVAIAAAVISDLGVA
WLPDWLVSKEIAQGQIQQILPECTSMKFAISAVWLEAAWMPQKIRIVIDELLAKLPEQIFYIPACGTRAE

Sequences:

>Translated_310_residues
MRTDLKGIPVFVAVVECGNFAQAAQDLNITRSAVGKAIARLEERLGVTLFQRTTRNQTLTEEGEILYQQSRIALDCIREA
EDVIHLGKTQVKGRLRISLPVLLGQRCIAPILLTIAKSYPQLKLELSFNDRQVNLFDEGFDLAVRIGELADSSFLQARRL
GQHGMILCASAQYLKTHLVPRTLSELQEHPAIGYQLGGKILNWQLNDMEGREINFRPNTVLAADDFVAIAAAVISDLGVA
WLPDWLVSKEIAQGQIQQILPECTSMKFAISAVWLEAAWMPQKIRIVIDELLAKLPEQIFYIPACGTRAE
>Mature_310_residues
MRTDLKGIPVFVAVVECGNFAQAAQDLNITRSAVGKAIARLEERLGVTLFQRTTRNQTLTEEGEILYQQSRIALDCIREA
EDVIHLGKTQVKGRLRISLPVLLGQRCIAPILLTIAKSYPQLKLELSFNDRQVNLFDEGFDLAVRIGELADSSFLQARRL
GQHGMILCASAQYLKTHLVPRTLSELQEHPAIGYQLGGKILNWQLNDMEGREINFRPNTVLAADDFVAIAAAVISDLGVA
WLPDWLVSKEIAQGQIQQILPECTSMKFAISAVWLEAAWMPQKIRIVIDELLAKLPEQIFYIPACGTRAE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=30.7432432432432, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789639, Length=287, Percent_Identity=29.616724738676, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.3972602739726, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=32.2033898305085, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=27.9310344827586, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=25.5172413793103, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787589, Length=265, Percent_Identity=29.0566037735849, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788706, Length=258, Percent_Identity=25.968992248062, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790011, Length=311, Percent_Identity=24.4372990353698, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1786448, Length=258, Percent_Identity=29.4573643410853, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI157672245, Length=121, Percent_Identity=33.8842975206612, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786713, Length=272, Percent_Identity=27.5735294117647, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082132, Length=265, Percent_Identity=28.3018867924528, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788481, Length=147, Percent_Identity=30.6122448979592, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787601, Length=142, Percent_Identity=28.8732394366197, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789173, Length=143, Percent_Identity=30.0699300699301, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790783, Length=270, Percent_Identity=24.8148148148148, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1788297, Length=145, Percent_Identity=28.2758620689655, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34575; Mature: 34575

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTDLKGIPVFVAVVECGNFAQAAQDLNITRSAVGKAIARLEERLGVTLFQRTTRNQTLT
CCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCC
EEGEILYQQSRIALDCIREAEDVIHLGKTQVKGRLRISLPVLLGQRCIAPILLTIAKSYP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCC
QLKLELSFNDRQVNLFDEGFDLAVRIGELADSSFLQARRLGQHGMILCASAQYLKTHLVP
CEEEEEEECCCEEEEECCCCEEEEEEHHHCCHHHHHHHHCCCCCEEEEECHHHHHHHCCH
RTLSELQEHPAIGYQLGGKILNWQLNDMEGREINFRPNTVLAADDFVAIAAAVISDLGVA
HHHHHHHHCCCCCEECCCEEEEEEECCCCCCEEECCCCEEEECCHHHHHHHHHHHHCCHH
WLPDWLVSKEIAQGQIQQILPECTSMKFAISAVWLEAAWMPQKIRIVIDELLAKLPEQIF
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHEE
YIPACGTRAE
EEECCCCCCC
>Mature Secondary Structure
MRTDLKGIPVFVAVVECGNFAQAAQDLNITRSAVGKAIARLEERLGVTLFQRTTRNQTLT
CCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCC
EEGEILYQQSRIALDCIREAEDVIHLGKTQVKGRLRISLPVLLGQRCIAPILLTIAKSYP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCC
QLKLELSFNDRQVNLFDEGFDLAVRIGELADSSFLQARRLGQHGMILCASAQYLKTHLVP
CEEEEEEECCCEEEEECCCCEEEEEEHHHCCHHHHHHHHCCCCCEEEEECHHHHHHHCCH
RTLSELQEHPAIGYQLGGKILNWQLNDMEGREINFRPNTVLAADDFVAIAAAVISDLGVA
HHHHHHHHCCCCCEECCCEEEEEEECCCCCCEEECCCCEEEECCHHHHHHHHHHHHCCHH
WLPDWLVSKEIAQGQIQQILPECTSMKFAISAVWLEAAWMPQKIRIVIDELLAKLPEQIF
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHEE
YIPACGTRAE
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]