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The map label for this gene is insF1 [H]

Identifier: 157372392

GI number: 157372392

Start: 4614400

End: 4615251

Strand: Reverse

Name: insF1 [H]

Synonym: Spro_4159

Alternate gene names: 157372392

Gene position: 4615251-4614400 (Counterclockwise)

Preceding gene: 157372393

Following gene: 157372391

Centisome position: 84.7

GC content: 48.59

Gene sequence:

>852_bases
ATGTCGCCATTTTACCCTGTACGCCGCCTATGCCTGGTTCTTGGCCTTCATTACAGCGGCTATTACCAGTGGCTAAAGCA
GCCAGAATCGTTGCGCGCCCGACAGGACGAACGTCAGACTGGCCTCATTAAGCAACTGTGGCTTGAAAGTGGTACGGTTT
ATGGTTACCGGAAAATCTGGCTCGATATGAAAGACCTGGGTGAACGCGCCGGACGTAACAGGGTTGCGCGGTTAATGAGG
CTCGCTGAATTAGCATCCCAGACCGGATATCGAAAACGACGTTACTACGGTGGTGGAAAACCTTCGGTAGCCAGCCCTAA
TTACCTTGAGCGACAGTTCATTGTCCCAACTCCAAATACGCATTGGGTGAGTGACATGACCTATATAAGGACGCATGAAG
GCTGGTTATATCTGGCTGTTGTTCTCGATCTCTTCTCACGTCGGGTTATAGGCTGGAGCATGGGAGGACGAATGACTGCT
GAGTTAGTGATGGATGCATTATTGATGGCGGTATGGCGACGTAAACCAACAGCAGAAGTATTAATCCATTCAGATCAGGG
TTCTCAATATACCAGCGAAAGGTGCCAGCATTTTATGGCATCTCACAATTTGAAAAGCAGCATGAGTCGTCGGGGTAATT
GCCATGACAACGCAGTGGCAGAAAGCTTTTTCCAGTTGCTAAAACGGGAACGCATAAAGAAACGGATCTATAAAAACAGA
GAAGACGCGAAGGCGGATATCTTCGATTATGTAGAGATGTTTTATAATGCTAAACGCCGCCACAGTGCCTGTGAGGATCA
ACCCCCTGCAGTTTATGAAAGTGCCTGGTTCATGAGGCACGGAACCGTCTAG

Upstream 100 bases:

>100_bases
GGCAAGAACTTAAGCGGGTTACTGAAGAGCGAGATATTTTAAAAAAGGCCGCCGCGTACTTTGCAAGAACATCCGGCTGA
AGTACGCCTTCATTAAAAAA

Downstream 100 bases:

>100_bases
GAAATCCGGGTCTATTCAGATCAGCACGCTGGTACGTGGCAAACTTCACCGATGAGCTGGCTACCAGGGAAAATACTGTT
ACCACAATCCGATAACAATA

Product: Integrase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSPFYPVRRLCLVLGLHYSGYYQWLKQPESLRARQDERQTGLIKQLWLESGTVYGYRKIWLDMKDLGERAGRNRVARLMR
LAELASQTGYRKRRYYGGGKPSVASPNYLERQFIVPTPNTHWVSDMTYIRTHEGWLYLAVVLDLFSRRVIGWSMGGRMTA
ELVMDALLMAVWRRKPTAEVLIHSDQGSQYTSERCQHFMASHNLKSSMSRRGNCHDNAVAESFFQLLKRERIKKRIYKNR
EDAKADIFDYVEMFYNAKRRHSACEDQPPAVYESAWFMRHGTV

Sequences:

>Translated_283_residues
MSPFYPVRRLCLVLGLHYSGYYQWLKQPESLRARQDERQTGLIKQLWLESGTVYGYRKIWLDMKDLGERAGRNRVARLMR
LAELASQTGYRKRRYYGGGKPSVASPNYLERQFIVPTPNTHWVSDMTYIRTHEGWLYLAVVLDLFSRRVIGWSMGGRMTA
ELVMDALLMAVWRRKPTAEVLIHSDQGSQYTSERCQHFMASHNLKSSMSRRGNCHDNAVAESFFQLLKRERIKKRIYKNR
EDAKADIFDYVEMFYNAKRRHSACEDQPPAVYESAWFMRHGTV
>Mature_282_residues
SPFYPVRRLCLVLGLHYSGYYQWLKQPESLRARQDERQTGLIKQLWLESGTVYGYRKIWLDMKDLGERAGRNRVARLMRL
AELASQTGYRKRRYYGGGKPSVASPNYLERQFIVPTPNTHWVSDMTYIRTHEGWLYLAVVLDLFSRRVIGWSMGGRMTAE
LVMDALLMAVWRRKPTAEVLIHSDQGSQYTSERCQHFMASHNLKSSMSRRGNCHDNAVAESFFQLLKRERIKKRIYKNRE
DAKADIFDYVEMFYNAKRRHSACEDQPPAVYESAWFMRHGTV

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=234, Percent_Identity=35.8974358974359, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI1788405, Length=274, Percent_Identity=40.1459854014599, Blast_Score=193, Evalue=9e-51,
Organism=Escherichia coli, GI1787263, Length=274, Percent_Identity=40.1459854014599, Blast_Score=193, Evalue=9e-51,
Organism=Escherichia coli, GI1786753, Length=274, Percent_Identity=40.1459854014599, Blast_Score=193, Evalue=9e-51,
Organism=Escherichia coli, GI1786570, Length=274, Percent_Identity=40.1459854014599, Blast_Score=193, Evalue=9e-51,
Organism=Escherichia coli, GI1786490, Length=274, Percent_Identity=40.1459854014599, Blast_Score=193, Evalue=9e-51,
Organism=Escherichia coli, GI1789981, Length=275, Percent_Identity=32.7272727272727, Blast_Score=141, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33281; Mature: 33149

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPFYPVRRLCLVLGLHYSGYYQWLKQPESLRARQDERQTGLIKQLWLESGTVYGYRKIW
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
LDMKDLGERAGRNRVARLMRLAELASQTGYRKRRYYGGGKPSVASPNYLERQFIVPTPNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHCCEEECCCCCC
HWVSDMTYIRTHEGWLYLAVVLDLFSRRVIGWSMGGRMTAELVMDALLMAVWRRKPTAEV
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHE
LIHSDQGSQYTSERCQHFMASHNLKSSMSRRGNCHDNAVAESFFQLLKRERIKKRIYKNR
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EDAKADIFDYVEMFYNAKRRHSACEDQPPAVYESAWFMRHGTV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SPFYPVRRLCLVLGLHYSGYYQWLKQPESLRARQDERQTGLIKQLWLESGTVYGYRKIW
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
LDMKDLGERAGRNRVARLMRLAELASQTGYRKRRYYGGGKPSVASPNYLERQFIVPTPNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHCCEEECCCCCC
HWVSDMTYIRTHEGWLYLAVVLDLFSRRVIGWSMGGRMTAELVMDALLMAVWRRKPTAEV
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHE
LIHSDQGSQYTSERCQHFMASHNLKSSMSRRGNCHDNAVAESFFQLLKRERIKKRIYKNR
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EDAKADIFDYVEMFYNAKRRHSACEDQPPAVYESAWFMRHGTV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]