Click here to switch to the map view.

The map label for this gene is topB [H]

Identifier: 157372341

GI number: 157372341

Start: 4561072

End: 4563111

Strand: Reverse

Name: topB [H]

Synonym: Spro_4107

Alternate gene names: 157372341

Gene position: 4563111-4561072 (Counterclockwise)

Preceding gene: 157372342

Following gene: 157372340

Centisome position: 83.74

GC content: 58.14

Gene sequence:

>2040_bases
ATGCAACTCTACCTGTGCGAGAAACCCAGCCAGGGTAAAGATATCGGCGCTATTCTCGGGGCGACGCAGCGTAAGCAGGG
TTACCTGGCGGGCGCCGGCGTCATTGTCACCTGGGCTATCGGTCACCTGCTGGAACAGGCCCAGCCGGAGACTTACGGTG
AGCAGTTCGGCAGTCCCTGGCGTGAGTCCGTTCTTCCTATCGTCCCTACCCAGTGGAAAATGACGGTCAAAAAAGAGACT
GCCGATCAGTTTGCTGTCATCCGCGGGTTGCTCAAACAGGTTGATTCTGTGGTGATTGCGACCGATGCAGACCGCGAGGG
GGAGGTGATCGCGAGAGAGTTACTGGAGCATTGCCACTTTACCGGCCCGGTCCAGCGCCTCTGGTTGTCAGCGCTGGACG
AGGCCAGTATTCGGCACGCCCTGGCGAACAAGCTCCCAGGCGAGAAGACTGCGCCCCTGTATGAAGCTGGCCTGGCGCGA
TCACGGGCTGACTGGATGACGGGGATGAATTTAACCCGACTTTACACGCTCAAAGCCCGTGAGCAGGGATACGGCGAGGT
GTTATCGATTGGGCGGGTGCAGACCCCTACGCTGGCGCTGGTCGTGAACCGTGACCGCGACATTGCCAACTTCATTTCCA
AACCGTACTGGCAAGTCAGGGCCACGCTGCAGAAGGGCGGGATCGCCTTCCCGGCAAGCTGGGTTCCTGCCGCCAACTAC
TGTGATGAGGAGAAGCGCTGTATTCAACAGAATGTGGCTCAAGCCGTTGTTCAGCTGTGTCAGCAAACAGGTCATGCCGT
TGTGCTTGAGGTGGGGACCGAGCGCAAGAAGGAGTCCGCGCCGTTGGCCTTTGACCTGGGCACCCTGCAGCAGGCTTGCT
CACGCAAGTGGGGGATGAGCGCCAACCAGGCCTTGAGTATCGCGCAGTCTCTGTATGAGACGCACAAAGCCACCACGTAT
CCCCGGACGGATTGCGGCTATCTGCCGACATCGATGCGGGAAGAAGTTGCCGATGTGCTGAGCGCGGTTACCCGCACGGA
CCCAACGCAGAGCGGCAGCGTTGCCAGGCTCGACAGGACATTTGTTTCTCGTATCTGGAATGACAAAAAAATCACGGCGC
ACCACGGGATTATCCCAACCAAACAGCCGTTCGACCTGAGCAAGCTGTCTGCCGACGAGCTTAAGGTTTATCAGCTGATC
CGTCAGCATTACCTGGCGCAGTTCCTGCCGTTACATGAAGTGGATGTGACGGAGGCTACTTTCAATATTGGCGGGCAACT
GTTCCGCACCCGCGGAAAGGTCGAGGTGGTGGCCGGGTGGAAAGTGCTGTTCCAGCATGACGTGAGCGAGGCGAAGGAGG
AATCTACCGACACAGATGATGTCCGGCTGCCAGCCCTGGTTAAAGGTGAAACCGTCGCGGTTGCCGGTGCTGAGCTCAAA
ACGCTGCAAACGAAGCCCCCAGGGCATTTCACCGACGGGACATTGATCGCCGCGATGAAAAATGCAGCGGCATTCGTCAG
TGATCCGCAACTGAAAAAGGTGTTGAAAGAGAACGCGGGGCTGGGGACGGAAGCGACGCGTGCCGGTATCCTGGAGACGC
TGTTTAAACGTGGGTACCTGGAGAAGAAGGGCAAATACCTGCTCTCTACGCCGATCGCCGGCGAACTGATAGATGCGTTA
CCGACCGCACTGACCAACCCCGGGATGACGGCGCTGTGGGAGCAGGCGTTGGACGAGATTGCTCAGGGTCAGATGACACT
CGACACATTCATGAGCCGTCAGGCGCAGTGGACAGGGCACCTGGTGGAGAAAGGAAAGGCGCAAGCCGTGAAGTTTACCG
CGCCACCTTCTCCACCTTGCCCGACCTGTAGCGGCACGATGCGCCGGCGTGCCGGCAAGAAAGGCAGTGGCGCCTTCTGG
GGCTGCAGTAATTACCCCACTTGCCAGGGCATCATTAACATCGATGCTAAAGGTGGGCGCAAGGCTAAGCGCTATAAAGC
AAAATCACCGGCTAAAAATAAAACAACCAAATCAGAGTAA

Upstream 100 bases:

>100_bases
CACCGGATGCTTCGCACCTGACCGATGCCTACCTCGGACGTTTGATGACGCAGCTGCAGCAGGTCGCGAAAGGTCAGCTG
GGCTGACGGGGGGTTATCTG

Downstream 100 bases:

>100_bases
GAGATTGTCTGATGTTAATCTCAGAAAGTTTTGCTAATGTGGGCGGGCTTTGATGCAGAGCACCCCGTGGCTGCGGATGG
CAGGAAAGTTTAATGCATGG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MQLYLCEKPSQGKDIGAILGATQRKQGYLAGAGVIVTWAIGHLLEQAQPETYGEQFGSPWRESVLPIVPTQWKMTVKKET
ADQFAVIRGLLKQVDSVVIATDADREGEVIARELLEHCHFTGPVQRLWLSALDEASIRHALANKLPGEKTAPLYEAGLAR
SRADWMTGMNLTRLYTLKAREQGYGEVLSIGRVQTPTLALVVNRDRDIANFISKPYWQVRATLQKGGIAFPASWVPAANY
CDEEKRCIQQNVAQAVVQLCQQTGHAVVLEVGTERKKESAPLAFDLGTLQQACSRKWGMSANQALSIAQSLYETHKATTY
PRTDCGYLPTSMREEVADVLSAVTRTDPTQSGSVARLDRTFVSRIWNDKKITAHHGIIPTKQPFDLSKLSADELKVYQLI
RQHYLAQFLPLHEVDVTEATFNIGGQLFRTRGKVEVVAGWKVLFQHDVSEAKEESTDTDDVRLPALVKGETVAVAGAELK
TLQTKPPGHFTDGTLIAAMKNAAAFVSDPQLKKVLKENAGLGTEATRAGILETLFKRGYLEKKGKYLLSTPIAGELIDAL
PTALTNPGMTALWEQALDEIAQGQMTLDTFMSRQAQWTGHLVEKGKAQAVKFTAPPSPPCPTCSGTMRRRAGKKGSGAFW
GCSNYPTCQGIINIDAKGGRKAKRYKAKSPAKNKTTKSE

Sequences:

>Translated_679_residues
MQLYLCEKPSQGKDIGAILGATQRKQGYLAGAGVIVTWAIGHLLEQAQPETYGEQFGSPWRESVLPIVPTQWKMTVKKET
ADQFAVIRGLLKQVDSVVIATDADREGEVIARELLEHCHFTGPVQRLWLSALDEASIRHALANKLPGEKTAPLYEAGLAR
SRADWMTGMNLTRLYTLKAREQGYGEVLSIGRVQTPTLALVVNRDRDIANFISKPYWQVRATLQKGGIAFPASWVPAANY
CDEEKRCIQQNVAQAVVQLCQQTGHAVVLEVGTERKKESAPLAFDLGTLQQACSRKWGMSANQALSIAQSLYETHKATTY
PRTDCGYLPTSMREEVADVLSAVTRTDPTQSGSVARLDRTFVSRIWNDKKITAHHGIIPTKQPFDLSKLSADELKVYQLI
RQHYLAQFLPLHEVDVTEATFNIGGQLFRTRGKVEVVAGWKVLFQHDVSEAKEESTDTDDVRLPALVKGETVAVAGAELK
TLQTKPPGHFTDGTLIAAMKNAAAFVSDPQLKKVLKENAGLGTEATRAGILETLFKRGYLEKKGKYLLSTPIAGELIDAL
PTALTNPGMTALWEQALDEIAQGQMTLDTFMSRQAQWTGHLVEKGKAQAVKFTAPPSPPCPTCSGTMRRRAGKKGSGAFW
GCSNYPTCQGIINIDAKGGRKAKRYKAKSPAKNKTTKSE
>Mature_679_residues
MQLYLCEKPSQGKDIGAILGATQRKQGYLAGAGVIVTWAIGHLLEQAQPETYGEQFGSPWRESVLPIVPTQWKMTVKKET
ADQFAVIRGLLKQVDSVVIATDADREGEVIARELLEHCHFTGPVQRLWLSALDEASIRHALANKLPGEKTAPLYEAGLAR
SRADWMTGMNLTRLYTLKAREQGYGEVLSIGRVQTPTLALVVNRDRDIANFISKPYWQVRATLQKGGIAFPASWVPAANY
CDEEKRCIQQNVAQAVVQLCQQTGHAVVLEVGTERKKESAPLAFDLGTLQQACSRKWGMSANQALSIAQSLYETHKATTY
PRTDCGYLPTSMREEVADVLSAVTRTDPTQSGSVARLDRTFVSRIWNDKKITAHHGIIPTKQPFDLSKLSADELKVYQLI
RQHYLAQFLPLHEVDVTEATFNIGGQLFRTRGKVEVVAGWKVLFQHDVSEAKEESTDTDDVRLPALVKGETVAVAGAELK
TLQTKPPGHFTDGTLIAAMKNAAAFVSDPQLKKVLKENAGLGTEATRAGILETLFKRGYLEKKGKYLLSTPIAGELIDAL
PTALTNPGMTALWEQALDEIAQGQMTLDTFMSRQAQWTGHLVEKGKAQAVKFTAPPSPPCPTCSGTMRRRAGKKGSGAFW
GCSNYPTCQGIINIDAKGGRKAKRYKAKSPAKNKTTKSE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=707, Percent_Identity=25.6011315417256, Blast_Score=146, Evalue=8e-35,
Organism=Homo sapiens, GI4507635, Length=600, Percent_Identity=24.5, Blast_Score=114, Evalue=4e-25,
Organism=Escherichia coli, GI1788061, Length=622, Percent_Identity=38.7459807073955, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1787529, Length=595, Percent_Identity=27.3949579831933, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=692, Percent_Identity=24.1329479768786, Blast_Score=125, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=640, Percent_Identity=24.53125, Blast_Score=124, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=672, Percent_Identity=23.9583333333333, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585251, Length=715, Percent_Identity=24.7552447552448, Blast_Score=144, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24640096, Length=621, Percent_Identity=24.7987117552335, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 74531; Mature: 74531

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLYLCEKPSQGKDIGAILGATQRKQGYLAGAGVIVTWAIGHLLEQAQPETYGEQFGSPW
CEEEEECCCCCCCCHHHHHCCCHHCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHCCCCH
RESVLPIVPTQWKMTVKKETADQFAVIRGLLKQVDSVVIATDADREGEVIARELLEHCHF
HHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCC
TGPVQRLWLSALDEASIRHALANKLPGEKTAPLYEAGLARSRADWMTGMNLTRLYTLKAR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHH
EQGYGEVLSIGRVQTPTLALVVNRDRDIANFISKPYWQVRATLQKGGIAFPASWVPAANY
HCCCHHHHHCCCCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHCCEECCCCCCCCCCC
CDEEKRCIQQNVAQAVVQLCQQTGHAVVLEVGTERKKESAPLAFDLGTLQQACSRKWGMS
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHCCCC
ANQALSIAQSLYETHKATTYPRTDCGYLPTSMREEVADVLSAVTRTDPTQSGSVARLDRT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
FVSRIWNDKKITAHHGIIPTKQPFDLSKLSADELKVYQLIRQHYLAQFLPLHEVDVTEAT
HHHHHCCCCEEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
FNIGGQLFRTRGKVEVVAGWKVLFQHDVSEAKEESTDTDDVRLPALVKGETVAVAGAELK
HHCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEECCEEECCCEEEEECCHHH
TLQTKPPGHFTDGTLIAAMKNAAAFVSDPQLKKVLKENAGLGTEATRAGILETLFKRGYL
EECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
EKKGKYLLSTPIAGELIDALPTALTNPGMTALWEQALDEIAQGQMTLDTFMSRQAQWTGH
HHCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LVEKGKAQAVKFTAPPSPPCPTCSGTMRRRAGKKGSGAFWGCSNYPTCQGIINIDAKGGR
HHHCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCCEECCCCCCCCCEEEEECCCCCC
KAKRYKAKSPAKNKTTKSE
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLYLCEKPSQGKDIGAILGATQRKQGYLAGAGVIVTWAIGHLLEQAQPETYGEQFGSPW
CEEEEECCCCCCCCHHHHHCCCHHCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHCCCCH
RESVLPIVPTQWKMTVKKETADQFAVIRGLLKQVDSVVIATDADREGEVIARELLEHCHF
HHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCC
TGPVQRLWLSALDEASIRHALANKLPGEKTAPLYEAGLARSRADWMTGMNLTRLYTLKAR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHH
EQGYGEVLSIGRVQTPTLALVVNRDRDIANFISKPYWQVRATLQKGGIAFPASWVPAANY
HCCCHHHHHCCCCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHCCEECCCCCCCCCCC
CDEEKRCIQQNVAQAVVQLCQQTGHAVVLEVGTERKKESAPLAFDLGTLQQACSRKWGMS
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHCCCC
ANQALSIAQSLYETHKATTYPRTDCGYLPTSMREEVADVLSAVTRTDPTQSGSVARLDRT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
FVSRIWNDKKITAHHGIIPTKQPFDLSKLSADELKVYQLIRQHYLAQFLPLHEVDVTEAT
HHHHHCCCCEEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
FNIGGQLFRTRGKVEVVAGWKVLFQHDVSEAKEESTDTDDVRLPALVKGETVAVAGAELK
HHCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEECCEEECCCEEEEECCHHH
TLQTKPPGHFTDGTLIAAMKNAAAFVSDPQLKKVLKENAGLGTEATRAGILETLFKRGYL
EECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
EKKGKYLLSTPIAGELIDALPTALTNPGMTALWEQALDEIAQGQMTLDTFMSRQAQWTGH
HHCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LVEKGKAQAVKFTAPPSPPCPTCSGTMRRRAGKKGSGAFWGCSNYPTCQGIINIDAKGGR
HHHCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCCEECCCCCCCCCEEEEECCCCCC
KAKRYKAKSPAKNKTTKSE
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]