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The map label for this gene is gatA [H]

Identifier: 157372138

GI number: 157372138

Start: 4323594

End: 4324943

Strand: Reverse

Name: gatA [H]

Synonym: Spro_3904

Alternate gene names: 157372138

Gene position: 4324943-4323594 (Counterclockwise)

Preceding gene: 157372139

Following gene: 157372137

Centisome position: 79.37

GC content: 61.78

Gene sequence:

>1350_bases
ATGAAAAAACTGACTTTAGCGCAGGCTGCCGCCGGATTGGCCAGTGGCGAATTCACTGCTGTGCAACTGACCGCCACCGC
TCTGGCGCAAATCGCCGACCCACAGGGGGAGGGCGCACGCACTTTTACTCATGTTTATAGCGACTGGGCACAGCAGCAGG
CATTGGCTGCCGATCGACGGCGTGCGGCGGGTAAGCCCCTGTCGGCGTTGGACGGTGTGCCGGTATCGGTCAAAGACCTG
TTTGATGTCGCCGGGGAGGCCACCGCTGCCGGTTCGCGGGTGTTGGCCGATGCGCCGGTAGCCAACAACCACGCTGCAGT
GGTGGCGCAGTTGTTGCAGGCTGGCGCGGTAGTGATCGGCAAAACCAACATGACCGAGTTTGCCTATTCGGGGCTTGGCA
TTAATCCCCACTACGGCACGCCCGCCAATCCCTGGGATCGCCCTGCTGGCCGTATTCCCGGTGGTTCGTCTTCCGGTGCG
GCGGTTGCGGTGGCTGACGGCATGTGCTTTGGCGCGATAGGCAGCGATACCGGCGGTTCGGTACGTATTCCGGCGGCCTT
CTGTGGCCTGACCGGTTATAAACCTACCGCCCGGCGCATCAGCAGTCGTGGTTTGCTGCCCTTGTCACCCTCACTGGATT
CAATTGGCGTGATTGCCCATGACGTTGCCGGTTGCATCGCGCTGGATACGGTGATTGCCCAGCAGCCGCTGCACCCGCAG
CAGAAAACGCTGAGTCAGGCGCGCTTTGCCGTGCCACAAACGTTGGCGCTGGATGGCCTGGATGAGGAAGTCACCGCCGC
CTTTCATCTCAGCGTGGAGCGGCTGGTGCAGGCGGGGGCACAGATTGAATTTATTCCCTGTCATGAGTTCGCCGAGCTGG
CAGCGATCAACGCCGCCGGTGGTTTCACCGCGCTGGAGTCCTGGCGCTGGCACCAGCCGCTGATTGCCGAACATGCCGAA
GACTATGACCCACGGGTGCTGTCACGTATTCGTCGCGGCCAGTTGCTCGATGAGCAGGATCTGCAGCAATTGCGGATGCA
GCGTGCCGATTGGCAACGGCGAGTGACCGCGGCAGTGGAAAAATTTGACGCGTTGTTGATGCCAACCCTGCCCTTCGTTG
CGCCGACCATTGCCGAACTGCAGGAGGATGAAGACGCTTATTTCCGTATCAACGGTGCCGCGTTGCGTAACCCTTCGGTG
ATCAACTTCCTCGATGGTTGTGCGCTGTCGCTTCCCTGTCAACGAGGTGATACGGCTCCTGTGGGGCTAATGGTGGCTGG
GCTGCCGCTGCATGACGAGGCGCTGCTGGGCTGGGCGCTGGCGATCGAACGCTGCCTCGCCGAGGCGTGA

Upstream 100 bases:

>100_bases
TCCGTCCGGCGGTCGAATTCCGTATGGAACTGGTGGACGAAGTGCTGGGACGTACCATCAGCCACGGTTATCGCAGCATA
GAACTGCCGGTGGTGGCGTG

Downstream 100 bases:

>100_bases
TTTTCCGGTGGGGACGACCCCGCCTTACTTTGAACTAACAGGAGAGTACCCATGACCGGTTCCCCAAACGGAATGCTGTT
TGTCGCAACAGATGTCGCCG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKKLTLAQAAAGLASGEFTAVQLTATALAQIADPQGEGARTFTHVYSDWAQQQALAADRRRAAGKPLSALDGVPVSVKDL
FDVAGEATAAGSRVLADAPVANNHAAVVAQLLQAGAVVIGKTNMTEFAYSGLGINPHYGTPANPWDRPAGRIPGGSSSGA
AVAVADGMCFGAIGSDTGGSVRIPAAFCGLTGYKPTARRISSRGLLPLSPSLDSIGVIAHDVAGCIALDTVIAQQPLHPQ
QKTLSQARFAVPQTLALDGLDEEVTAAFHLSVERLVQAGAQIEFIPCHEFAELAAINAAGGFTALESWRWHQPLIAEHAE
DYDPRVLSRIRRGQLLDEQDLQQLRMQRADWQRRVTAAVEKFDALLMPTLPFVAPTIAELQEDEDAYFRINGAALRNPSV
INFLDGCALSLPCQRGDTAPVGLMVAGLPLHDEALLGWALAIERCLAEA

Sequences:

>Translated_449_residues
MKKLTLAQAAAGLASGEFTAVQLTATALAQIADPQGEGARTFTHVYSDWAQQQALAADRRRAAGKPLSALDGVPVSVKDL
FDVAGEATAAGSRVLADAPVANNHAAVVAQLLQAGAVVIGKTNMTEFAYSGLGINPHYGTPANPWDRPAGRIPGGSSSGA
AVAVADGMCFGAIGSDTGGSVRIPAAFCGLTGYKPTARRISSRGLLPLSPSLDSIGVIAHDVAGCIALDTVIAQQPLHPQ
QKTLSQARFAVPQTLALDGLDEEVTAAFHLSVERLVQAGAQIEFIPCHEFAELAAINAAGGFTALESWRWHQPLIAEHAE
DYDPRVLSRIRRGQLLDEQDLQQLRMQRADWQRRVTAAVEKFDALLMPTLPFVAPTIAELQEDEDAYFRINGAALRNPSV
INFLDGCALSLPCQRGDTAPVGLMVAGLPLHDEALLGWALAIERCLAEA
>Mature_449_residues
MKKLTLAQAAAGLASGEFTAVQLTATALAQIADPQGEGARTFTHVYSDWAQQQALAADRRRAAGKPLSALDGVPVSVKDL
FDVAGEATAAGSRVLADAPVANNHAAVVAQLLQAGAVVIGKTNMTEFAYSGLGINPHYGTPANPWDRPAGRIPGGSSSGA
AVAVADGMCFGAIGSDTGGSVRIPAAFCGLTGYKPTARRISSRGLLPLSPSLDSIGVIAHDVAGCIALDTVIAQQPLHPQ
QKTLSQARFAVPQTLALDGLDEEVTAAFHLSVERLVQAGAQIEFIPCHEFAELAAINAAGGFTALESWRWHQPLIAEHAE
DYDPRVLSRIRRGQLLDEQDLQQLRMQRADWQRRVTAAVEKFDALLMPTLPFVAPTIAELQEDEDAYFRINGAALRNPSV
INFLDGCALSLPCQRGDTAPVGLMVAGLPLHDEALLGWALAIERCLAEA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=27.5590551181102, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI195972892, Length=138, Percent_Identity=38.4057971014493, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI166795287, Length=136, Percent_Identity=34.5588235294118, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=416, Percent_Identity=27.6442307692308, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=161, Percent_Identity=41.6149068322981, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=172, Percent_Identity=36.6279069767442, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=157, Percent_Identity=36.3057324840764, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=198, Percent_Identity=35.3535353535354, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=198, Percent_Identity=35.3535353535354, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=216, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=215, Percent_Identity=27.4418604651163, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=387, Percent_Identity=31.266149870801, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323950, Length=224, Percent_Identity=32.5892857142857, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648113, Length=482, Percent_Identity=31.1203319502075, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45550774, Length=143, Percent_Identity=37.7622377622378, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648435, Length=143, Percent_Identity=37.7622377622378, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648437, Length=143, Percent_Identity=37.7622377622378, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648441, Length=143, Percent_Identity=37.7622377622378, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648439, Length=143, Percent_Identity=37.7622377622378, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356731, Length=128, Percent_Identity=38.28125, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644968, Length=217, Percent_Identity=29.0322580645161, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652985, Length=138, Percent_Identity=36.231884057971, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922090, Length=138, Percent_Identity=36.231884057971, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652981, Length=138, Percent_Identity=36.231884057971, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652983, Length=138, Percent_Identity=36.231884057971, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 47340; Mature: 47340

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTLAQAAAGLASGEFTAVQLTATALAQIADPQGEGARTFTHVYSDWAQQQALAADRR
CCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RAAGKPLSALDGVPVSVKDLFDVAGEATAAGSRVLADAPVANNHAAVVAQLLQAGAVVIG
HHCCCCCHHHCCCCCCHHHHHHHHCCHHHCCCEEEECCCCCCCHHHHHHHHHHCCEEEEE
KTNMTEFAYSGLGINPHYGTPANPWDRPAGRIPGGSSSGAAVAVADGMCFGAIGSDTGGS
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCCCCCCC
VRIPAAFCGLTGYKPTARRISSRGLLPLSPSLDSIGVIAHDVAGCIALDTVIAQQPLHPQ
EECCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
QKTLSQARFAVPQTLALDGLDEEVTAAFHLSVERLVQAGAQIEFIPCHEFAELAAINAAG
HHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCC
GFTALESWRWHQPLIAEHAEDYDPRVLSRIRRGQLLDEQDLQQLRMQRADWQRRVTAAVE
CHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KFDALLMPTLPFVAPTIAELQEDEDAYFRINGAALRNPSVINFLDGCALSLPCQRGDTAP
HHHHHHHCHHHHHHHHHHHHCCCCCCEEEECCCEECCCCHHHHHCCCEEECCCCCCCCCC
VGLMVAGLPLHDEALLGWALAIERCLAEA
CEEEEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLTLAQAAAGLASGEFTAVQLTATALAQIADPQGEGARTFTHVYSDWAQQQALAADRR
CCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RAAGKPLSALDGVPVSVKDLFDVAGEATAAGSRVLADAPVANNHAAVVAQLLQAGAVVIG
HHCCCCCHHHCCCCCCHHHHHHHHCCHHHCCCEEEECCCCCCCHHHHHHHHHHCCEEEEE
KTNMTEFAYSGLGINPHYGTPANPWDRPAGRIPGGSSSGAAVAVADGMCFGAIGSDTGGS
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCCCCCCC
VRIPAAFCGLTGYKPTARRISSRGLLPLSPSLDSIGVIAHDVAGCIALDTVIAQQPLHPQ
EECCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
QKTLSQARFAVPQTLALDGLDEEVTAAFHLSVERLVQAGAQIEFIPCHEFAELAAINAAG
HHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCC
GFTALESWRWHQPLIAEHAEDYDPRVLSRIRRGQLLDEQDLQQLRMQRADWQRRVTAAVE
CHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KFDALLMPTLPFVAPTIAELQEDEDAYFRINGAALRNPSVINFLDGCALSLPCQRGDTAP
HHHHHHHCHHHHHHHHHHHHCCCCCCEEEECCCEECCCCHHHHHCCCEEECCCCCCCCCC
VGLMVAGLPLHDEALLGWALAIERCLAEA
CEEEEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA