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The map label for this gene is yadI [H]

Identifier: 157372079

GI number: 157372079

Start: 4255292

End: 4255726

Strand: Reverse

Name: yadI [H]

Synonym: Spro_3844

Alternate gene names: 157372079

Gene position: 4255726-4255292 (Counterclockwise)

Preceding gene: 157372080

Following gene: 157372077

Centisome position: 78.1

GC content: 60.69

Gene sequence:

>435_bases
ATGCCAGGCATTGTGATTACCGGTCACGGCGGTTTCGCCAGTGGGCTGTTGCAGGCGGTTGAACAGGTGGTGGGGCCGCA
GCCGTACTGCGTTGCGGTCAATTTTCCCGAGCAGATGAGTACTGCCGAGTTAAAAGCGGCGTTGAGCTCGGCGTTGGCCG
AAGTGGCACAGCCGGACGGCGTGGTGTTTCTCACCGATATGCTCGGCGGTTCGCCGTTTCGCTGTGCCAGCGAACTGGCG
GATACGCACGGGGATTGTGAGGTGCTGACCGGGGTGAATATGCAGTTGGCGGCGGAAATGATGCTGGAAAGGGACGGGCT
GAGCCTGGACGAATTCCGCGAAGTGGCACTGGCGTGCGGTAAGCGCGGGTTGACCAGCCTGTGGCACGAAAGACGCCGGG
TAAAATGTGAGGACGTACAGACCGACGGCATTTGA

Upstream 100 bases:

>100_bases
TTCCTGATGAAGCGCGGCTTCTCACCGGTGAAACTGATCGGTGTGACGGTGGTGATTGGTGTGGTGGGCAAGTTTATCGG
TTTATTGTGAGGAGCGGTTT

Downstream 100 bases:

>100_bases
GGCAAGCCGGGTGACGCTGCGATGAACACCACCCGGTTGATTAACCGACGACCGGCAGCGGGCTAAGCGCCATGCGGGCC
GGTTGATTCTCCTCCGGGCG

Product: PTS system fructose subfamily transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MPGIVITGHGGFASGLLQAVEQVVGPQPYCVAVNFPEQMSTAELKAALSSALAEVAQPDGVVFLTDMLGGSPFRCASELA
DTHGDCEVLTGVNMQLAAEMMLERDGLSLDEFREVALACGKRGLTSLWHERRRVKCEDVQTDGI

Sequences:

>Translated_144_residues
MPGIVITGHGGFASGLLQAVEQVVGPQPYCVAVNFPEQMSTAELKAALSSALAEVAQPDGVVFLTDMLGGSPFRCASELA
DTHGDCEVLTGVNMQLAAEMMLERDGLSLDEFREVALACGKRGLTSLWHERRRVKCEDVQTDGI
>Mature_143_residues
PGIVITGHGGFASGLLQAVEQVVGPQPYCVAVNFPEQMSTAELKAALSSALAEVAQPDGVVFLTDMLGGSPFRCASELAD
THGDCEVLTGVNMQLAAEMMLERDGLSLDEFREVALACGKRGLTSLWHERRRVKCEDVQTDGI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1786321, Length=133, Percent_Identity=39.0977443609023, Blast_Score=96, Evalue=7e-22,
Organism=Escherichia coli, GI1788120, Length=126, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701 [H]

Pfam domain/function: PF03610 EIIA-man [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15338; Mature: 15207

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGIVITGHGGFASGLLQAVEQVVGPQPYCVAVNFPEQMSTAELKAALSSALAEVAQPDG
CCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCC
VVFLTDMLGGSPFRCASELADTHGDCEVLTGVNMQLAAEMMLERDGLSLDEFREVALACG
EEEEHHHHCCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHC
KRGLTSLWHERRRVKCEDVQTDGI
CCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PGIVITGHGGFASGLLQAVEQVVGPQPYCVAVNFPEQMSTAELKAALSSALAEVAQPDG
CCEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCC
VVFLTDMLGGSPFRCASELADTHGDCEVLTGVNMQLAAEMMLERDGLSLDEFREVALACG
EEEEHHHHCCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHC
KRGLTSLWHERRRVKCEDVQTDGI
CCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503 [H]