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The map label for this gene is truC [H]

Identifier: 157372031

GI number: 157372031

Start: 4196562

End: 4197332

Strand: Reverse

Name: truC [H]

Synonym: Spro_3796

Alternate gene names: 157372031

Gene position: 4197332-4196562 (Counterclockwise)

Preceding gene: 157372032

Following gene: 157372030

Centisome position: 77.03

GC content: 57.98

Gene sequence:

>771_bases
ATGCTTGAGATTCTATATCAGGATGAGCATTTGGTGGCGGTGAACAAGCCTTCCGGGTGGTTGGTACACCGCAGCTGGCT
GGATCGCCATGAAACCCGGTTTGTGATGCAAACCGTGCGCGATCAGATTGGCCAGCATGTGTTTACCGTGCATCGCCTCG
ATCGGCCCACCTCAGGGGTGCTGCTGATGGCGCTGTCCAGTGAGGTGGCACGGTTGCTGTCGCAGCAGTTTGAACAGCAT
CAGATGCAGAAAACCTATCACGCGGTGGTGCGTGGTTACGTGCTGGAGGGGGCGACCATCGACTATCCGCTGACGGAAGA
GCTGGACAAAATCGCCGACAAGTTTGCCTCTAAAGACAAAGGCCCGCAGCCGGCGGTCACTCATTACCGGCCGCTGGCGC
GGGTAGAAATGCCGGTGGCGATTGGCCGTTACGAAAGCGCGCGCTACAGCCTGGTTGAACTGAAGCCGGAAAATGGCCGC
AAGCACCAGTTGCGCCGCCATATGGTGCATATCCGCCATCCGATTATTGGTGACAGCAAACACGGCGACCTGAACCAGAA
CCGGGGTATGGCCCAGCATTTTGGCTGTCCACGCCTGATGCTGCATGCCAGTCATCTGCAACTGAATCATCCGGTGACCG
GTGAGCCGCTGCATCTTTCCGCGCGCTGGGATGAACCCTGGCAGGGAGTCATGTCACAGTTTGGCTGGACAGAGAATCTC
CCTGAGCTTGCCAGGGTTGAGTTTCCCCCGGCTAGCGGTCAGGATAACTGA

Upstream 100 bases:

>100_bases
CTTATTTCGAAGAGGCGTTGAAAGATAACGACCCCAACTGCATGCCGCTGTTGGTGCTGTTGCAGCGCCTGGATAACATG
CTGAATAAAGAGCTGCAATA

Downstream 100 bases:

>100_bases
TCATTTTCAAAGATATTTAAGGGAGTAGGGGCAATGGCTCAGGTTGGAATCTTCGTGGGAACGGTCTACGGCAACGCATT
ATTGGTGGCGGAAGAGGCTG

Product: tRNA pseudouridine synthase C

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLEILYQDEHLVAVNKPSGWLVHRSWLDRHETRFVMQTVRDQIGQHVFTVHRLDRPTSGVLLMALSSEVARLLSQQFEQH
QMQKTYHAVVRGYVLEGATIDYPLTEELDKIADKFASKDKGPQPAVTHYRPLARVEMPVAIGRYESARYSLVELKPENGR
KHQLRRHMVHIRHPIIGDSKHGDLNQNRGMAQHFGCPRLMLHASHLQLNHPVTGEPLHLSARWDEPWQGVMSQFGWTENL
PELARVEFPPASGQDN

Sequences:

>Translated_256_residues
MLEILYQDEHLVAVNKPSGWLVHRSWLDRHETRFVMQTVRDQIGQHVFTVHRLDRPTSGVLLMALSSEVARLLSQQFEQH
QMQKTYHAVVRGYVLEGATIDYPLTEELDKIADKFASKDKGPQPAVTHYRPLARVEMPVAIGRYESARYSLVELKPENGR
KHQLRRHMVHIRHPIIGDSKHGDLNQNRGMAQHFGCPRLMLHASHLQLNHPVTGEPLHLSARWDEPWQGVMSQFGWTENL
PELARVEFPPASGQDN
>Mature_256_residues
MLEILYQDEHLVAVNKPSGWLVHRSWLDRHETRFVMQTVRDQIGQHVFTVHRLDRPTSGVLLMALSSEVARLLSQQFEQH
QMQKTYHAVVRGYVLEGATIDYPLTEELDKIADKFASKDKGPQPAVTHYRPLARVEMPVAIGRYESARYSLVELKPENGR
KHQLRRHMVHIRHPIIGDSKHGDLNQNRGMAQHFGCPRLMLHASHLQLNHPVTGEPLHLSARWDEPWQGVMSQFGWTENL
PELARVEFPPASGQDN

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI221316667, Length=189, Percent_Identity=30.6878306878307, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI23308689, Length=178, Percent_Identity=29.2134831460674, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI17158025, Length=223, Percent_Identity=27.3542600896861, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221316663, Length=207, Percent_Identity=28.0193236714976, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1789155, Length=256, Percent_Identity=74.609375, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1786244, Length=224, Percent_Identity=35.2678571428571, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787327, Length=233, Percent_Identity=33.0472103004292, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788946, Length=262, Percent_Identity=28.2442748091603, Blast_Score=92, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324506, Length=180, Percent_Identity=27.2222222222222, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320168, Length=180, Percent_Identity=27.2222222222222, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321608, Length=195, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921090, Length=179, Percent_Identity=31.2849162011173, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583449, Length=179, Percent_Identity=31.8435754189944, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583447, Length=179, Percent_Identity=31.8435754189944, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583445, Length=179, Percent_Identity=31.8435754189944, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 29482; Mature: 29482

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEILYQDEHLVAVNKPSGWLVHRSWLDRHETRFVMQTVRDQIGQHVFTVHRLDRPTSGV
CCEEEEECCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
LLMALSSEVARLLSQQFEQHQMQKTYHAVVRGYVLEGATIDYPLTEELDKIADKFASKDK
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCC
GPQPAVTHYRPLARVEMPVAIGRYESARYSLVELKPENGRKHQLRRHMVHIRHPIIGDSK
CCCCCHHHCCCHHHEECCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
HGDLNQNRGMAQHFGCPRLMLHASHLQLNHPVTGEPLHLSARWDEPWQGVMSQFGWTENL
CCCCCCCCCCHHHCCCCHHEEEHHHEEECCCCCCCCEEEECCCCCHHHHHHHHCCCCCCC
PELARVEFPPASGQDN
CHHHEEECCCCCCCCC
>Mature Secondary Structure
MLEILYQDEHLVAVNKPSGWLVHRSWLDRHETRFVMQTVRDQIGQHVFTVHRLDRPTSGV
CCEEEEECCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
LLMALSSEVARLLSQQFEQHQMQKTYHAVVRGYVLEGATIDYPLTEELDKIADKFASKDK
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCC
GPQPAVTHYRPLARVEMPVAIGRYESARYSLVELKPENGRKHQLRRHMVHIRHPIIGDSK
CCCCCHHHCCCHHHEECCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
HGDLNQNRGMAQHFGCPRLMLHASHLQLNHPVTGEPLHLSARWDEPWQGVMSQFGWTENL
CCCCCCCCCCHHHCCCCHHEEEHHHEEECCCCCCCCEEEECCCCCHHHHHHHHCCCCCCC
PELARVEFPPASGQDN
CHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]