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The map label for this gene is rnhB

Identifier: 157372011

GI number: 157372011

Start: 4175520

End: 4176113

Strand: Reverse

Name: rnhB

Synonym: Spro_3776

Alternate gene names: 157372011

Gene position: 4176113-4175520 (Counterclockwise)

Preceding gene: 157372012

Following gene: 157372010

Centisome position: 76.64

GC content: 59.09

Gene sequence:

>594_bases
ATGATTGAACCCTTCATTTATCCTGTCGCCACGCTGATTGCCGGCGTGGACGAAGTCGGCCGTGGCCCTTTGGTCGGGGC
GGTAGTGACCGCCGCAGTGATCCTCGATCCTGCGCGACCAATCGTTGGGCTGGCGGACTCGAAAAAACTCAGTGAAAAGC
GCCGCCTGGCGCTGTATGACGAAATCGTCGAAAAGGCACTTTCCTGGAGTCTGGGGCGAGCCGAGCCGGACGAGATCGAT
CAACTGAACATTCTGCATGCCACCATGCTGGCGATGCAGCGAGCGGTTGCCGGATTGCATATCGCGCCGGACATGGTATT
GATCGACGGTAACCGTTGTCCTAAATTACCGATGCGTTCGCAGGCGGTGGTGAAGGGCGATAGCCGCGTGGCAGAAATCA
GCGCCGCGTCCATTCTGGCGAAGGTAACGCGTGATCGCGAAATGACCGAGTTGGATCGGGAATTCCCGGACTACGGTTTT
GCGCAGCATAAAGGCTACCCAACCGCTTTCCATCTGGAGCGGCTGGCCGCACTGGGTGCGACCGAGCATCATCGCCGCAG
CTTTGCCCCGGTTAGAAGGGTGCTGGCGCTGTAA

Upstream 100 bases:

>100_bases
AAACCGACGAACTGAAACAGACTTTCCTTACCCTGCACCAAAGCATCCGTTGTGGTGCCGATGAACAGGCCGCTCAGGCT
GTGTTGGAGCTGGCAAAAGC

Downstream 100 bases:

>100_bases
TCTGCCGGCCCTAGCGATAGAGTTAACCCGGGTTCAGTTCGCTGAACCCCTAGATTCAGGTATCTGGATATGGCCGAACC
TCGTTTTATTCACCTGCGCG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MIEPFIYPVATLIAGVDEVGRGPLVGAVVTAAVILDPARPIVGLADSKKLSEKRRLALYDEIVEKALSWSLGRAEPDEID
QLNILHATMLAMQRAVAGLHIAPDMVLIDGNRCPKLPMRSQAVVKGDSRVAEISAASILAKVTRDREMTELDREFPDYGF
AQHKGYPTAFHLERLAALGATEHHRRSFAPVRRVLAL

Sequences:

>Translated_197_residues
MIEPFIYPVATLIAGVDEVGRGPLVGAVVTAAVILDPARPIVGLADSKKLSEKRRLALYDEIVEKALSWSLGRAEPDEID
QLNILHATMLAMQRAVAGLHIAPDMVLIDGNRCPKLPMRSQAVVKGDSRVAEISAASILAKVTRDREMTELDREFPDYGF
AQHKGYPTAFHLERLAALGATEHHRRSFAPVRRVLAL
>Mature_197_residues
MIEPFIYPVATLIAGVDEVGRGPLVGAVVTAAVILDPARPIVGLADSKKLSEKRRLALYDEIVEKALSWSLGRAEPDEID
QLNILHATMLAMQRAVAGLHIAPDMVLIDGNRCPKLPMRSQAVVKGDSRVAEISAASILAKVTRDREMTELDREFPDYGF
AQHKGYPTAFHLERLAALGATEHHRRSFAPVRRVLAL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=197, Percent_Identity=84.2639593908629, Blast_Score=330, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_SERP5 (A8GID2)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480000.1
- ProteinModelPortal:   A8GID2
- SMR:   A8GID2
- STRING:   A8GID2
- GeneID:   5607352
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3776
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   SPRO399741:SPRO_3776-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21580; Mature: 21580

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEPFIYPVATLIAGVDEVGRGPLVGAVVTAAVILDPARPIVGLADSKKLSEKRRLALYD
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHH
EIVEKALSWSLGRAEPDEIDQLNILHATMLAMQRAVAGLHIAPDMVLIDGNRCPKLPMRS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCEEEECCCCCCCCCCCC
QAVVKGDSRVAEISAASILAKVTRDREMTELDREFPDYGFAQHKGYPTAFHLERLAALGA
CHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHCC
TEHHRRSFAPVRRVLAL
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIEPFIYPVATLIAGVDEVGRGPLVGAVVTAAVILDPARPIVGLADSKKLSEKRRLALYD
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHH
EIVEKALSWSLGRAEPDEIDQLNILHATMLAMQRAVAGLHIAPDMVLIDGNRCPKLPMRS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCEEEECCCCCCCCCCCC
QAVVKGDSRVAEISAASILAKVTRDREMTELDREFPDYGFAQHKGYPTAFHLERLAALGA
CHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHCC
TEHHRRSFAPVRRVLAL
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA