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The map label for this gene is tilS

Identifier: 157372000

GI number: 157372000

Start: 4158802

End: 4160112

Strand: Reverse

Name: tilS

Synonym: Spro_3765

Alternate gene names: 157372000

Gene position: 4160112-4158802 (Counterclockwise)

Preceding gene: 157372001

Following gene: 157371999

Centisome position: 76.35

GC content: 57.97

Gene sequence:

>1311_bases
ATGAATAACAATCAATTATCCGCACAGCTAGCCCGCCAATTGGGTGCACACCGGCACTTGCTGGTGGCATTCAGTGGCGG
GCTGGACTCCAGCGTGCTATTGCATCTGCTGGTAGGGTTACGGCAGCAATTGCCGGACTTGCAGCTTCGCGCGGTGCACG
TGCATCACGGCCTGAGCGCTTTTGCCGACCAGTGGGTAACACACTGTCGGCAACAATGCGTTGCCTGGCAGCTCCCCCTG
GTGGTTCAGCACGTGCAGGTTGATAGCCAGCAGGGCGGTATCGAAGCCGCAGCCCGGGCGGCACGTTACTCGGCTTTTGC
CAGTACGCTGGCGGCTGATGAAACCTTGCTTACCGCGCAACACCTTGACGACCAATGTGAAACCTTCCTGCTGGCGCTAA
AACGTGGCAGTGGCCCGGCGGGCCTGTCGGCCATGGCTGCACAGACGTCGCTGGGGAATAATCATTTGCTGCGCCCACTG
TTGGGTCACTCTCGCCAGCAGTTGGAGGCCTATGCGCAGCAACATCAGCTCAGTTGGATTGAAGACGACAGCAATCAGGA
CCCACGCTTCGACCGCAACTTTTTGCGCCTGCAGGTGTTGCCGCTACTGAATCAGCGCTGGCCGCACTTTGCTGCAGCGA
CGGCACGTAGTGCCAGCCTGTGCGCCGAGCAAGAGCAATTGCTGGATGAATTGCTGGCAGAGCAACTGCACAATCTGCTC
GATGAAGATCGTGCGTTGGCGATTGATGGCCTGCTGACATGCTCAGCGGCCCGACGTTTCGCCCTGTTACGCCGTTGGAT
CGCGCTATTTGGCGTTACCATGCCTTCTCGCGAACAGTTGCAGCGGTTATGGGAAGAAGTGGCGCTTAGCCGCGAAGACG
CCGAACCCCAGCTGCAATTGGGACAATATCAGTTTCGCCGCTTCCGCCGCCGCTTGTATCTGCTGCCGCTGATGGCCGAT
CTGCGTGAAATCAGTCTAAGCTGGTCATTGGATGATTCGCTGATGCTGCCGGATGGGTTGGGTGAACTGGTCAGTGGGGA
AGGAGACATCTGTTTGCGTGCGCCGCAATCACAGCAAAAAGTGAGCATTCGTTTTAGCGCTCAGGGCAAACACCGCATCC
TGGGGCGCGCGCATTCTCGTCCGATCAAAAAATTGTGGCAGGAGTTGGGTATTCCTCCGTGGCAGCGTGAGCGTATCCCG
TTGATCTATTATGATGACCAGCTGATTGCGGCGCTGGGGATTTTTGTCAGCGAGGCCGGACAAACGCCTGAGGGGCAGCA
GCCCTGGCGGCTGCACTGGCGTAAAAAATAA

Upstream 100 bases:

>100_bases
GGTGGTTGCTGTCCGGCCGTCATATCCGTGCGGTTAATGCTTGATTGATGGTATGACGGGGGTTAACGTGCAGCTATCTG
GACGTCATTAAGCCTAATCA

Downstream 100 bases:

>100_bases
TAATTAGACTGAGGAACCATGATGAAATTTGTGAGCGCAATGGCGGTGATGTTGGGTCTGTTCAGCCTGCCTGTAATGGC
GGCGGGCGATGCGGCTGCGG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNNNQLSAQLARQLGAHRHLLVAFSGGLDSSVLLHLLVGLRQQLPDLQLRAVHVHHGLSAFADQWVTHCRQQCVAWQLPL
VVQHVQVDSQQGGIEAAARAARYSAFASTLAADETLLTAQHLDDQCETFLLALKRGSGPAGLSAMAAQTSLGNNHLLRPL
LGHSRQQLEAYAQQHQLSWIEDDSNQDPRFDRNFLRLQVLPLLNQRWPHFAAATARSASLCAEQEQLLDELLAEQLHNLL
DEDRALAIDGLLTCSAARRFALLRRWIALFGVTMPSREQLQRLWEEVALSREDAEPQLQLGQYQFRRFRRRLYLLPLMAD
LREISLSWSLDDSLMLPDGLGELVSGEGDICLRAPQSQQKVSIRFSAQGKHRILGRAHSRPIKKLWQELGIPPWQRERIP
LIYYDDQLIAALGIFVSEAGQTPEGQQPWRLHWRKK

Sequences:

>Translated_436_residues
MNNNQLSAQLARQLGAHRHLLVAFSGGLDSSVLLHLLVGLRQQLPDLQLRAVHVHHGLSAFADQWVTHCRQQCVAWQLPL
VVQHVQVDSQQGGIEAAARAARYSAFASTLAADETLLTAQHLDDQCETFLLALKRGSGPAGLSAMAAQTSLGNNHLLRPL
LGHSRQQLEAYAQQHQLSWIEDDSNQDPRFDRNFLRLQVLPLLNQRWPHFAAATARSASLCAEQEQLLDELLAEQLHNLL
DEDRALAIDGLLTCSAARRFALLRRWIALFGVTMPSREQLQRLWEEVALSREDAEPQLQLGQYQFRRFRRRLYLLPLMAD
LREISLSWSLDDSLMLPDGLGELVSGEGDICLRAPQSQQKVSIRFSAQGKHRILGRAHSRPIKKLWQELGIPPWQRERIP
LIYYDDQLIAALGIFVSEAGQTPEGQQPWRLHWRKK
>Mature_436_residues
MNNNQLSAQLARQLGAHRHLLVAFSGGLDSSVLLHLLVGLRQQLPDLQLRAVHVHHGLSAFADQWVTHCRQQCVAWQLPL
VVQHVQVDSQQGGIEAAARAARYSAFASTLAADETLLTAQHLDDQCETFLLALKRGSGPAGLSAMAAQTSLGNNHLLRPL
LGHSRQQLEAYAQQHQLSWIEDDSNQDPRFDRNFLRLQVLPLLNQRWPHFAAATARSASLCAEQEQLLDELLAEQLHNLL
DEDRALAIDGLLTCSAARRFALLRRWIALFGVTMPSREQLQRLWEEVALSREDAEPQLQLGQYQFRRFRRRLYLLPLMAD
LREISLSWSLDDSLMLPDGLGELVSGEGDICLRAPQSQQKVSIRFSAQGKHRILGRAHSRPIKKLWQELGIPPWQRERIP
LIYYDDQLIAALGIFVSEAGQTPEGQQPWRLHWRKK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=433, Percent_Identity=54.0415704387991, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_SERP5 (A8GIC1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479989.1
- ProteinModelPortal:   A8GIC1
- SMR:   A8GIC1
- STRING:   A8GIC1
- GeneID:   5603196
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3765
- eggNOG:   COG0037
- HOGENOM:   HBG668702
- OMA:   WWIKSVA
- ProtClustDB:   PRK10660
- BioCyc:   SPRO399741:SPRO_3765-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 49358; Mature: 49358

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNQLSAQLARQLGAHRHLLVAFSGGLDSSVLLHLLVGLRQQLPDLQLRAVHVHHGLSA
CCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHCHHH
FADQWVTHCRQQCVAWQLPLVVQHVQVDSQQGGIEAAARAARYSAFASTLAADETLLTAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLDDQCETFLLALKRGSGPAGLSAMAAQTSLGNNHLLRPLLGHSRQQLEAYAQQHQLSWI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCC
EDDSNQDPRFDRNFLRLQVLPLLNQRWPHFAAATARSASLCAEQEQLLDELLAEQLHNLL
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEDRALAIDGLLTCSAARRFALLRRWIALFGVTMPSREQLQRLWEEVALSREDAEPQLQL
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
GQYQFRRFRRRLYLLPLMADLREISLSWSLDDSLMLPDGLGELVSGEGDICLRAPQSQQK
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEECCCCHHHHHCCCCCEEEECCCCCCE
VSIRFSAQGKHRILGRAHSRPIKKLWQELGIPPWQRERIPLIYYDDQLIAALGIFVSEAG
EEEEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCC
QTPEGQQPWRLHWRKK
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNNQLSAQLARQLGAHRHLLVAFSGGLDSSVLLHLLVGLRQQLPDLQLRAVHVHHGLSA
CCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHCHHH
FADQWVTHCRQQCVAWQLPLVVQHVQVDSQQGGIEAAARAARYSAFASTLAADETLLTAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLDDQCETFLLALKRGSGPAGLSAMAAQTSLGNNHLLRPLLGHSRQQLEAYAQQHQLSWI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCC
EDDSNQDPRFDRNFLRLQVLPLLNQRWPHFAAATARSASLCAEQEQLLDELLAEQLHNLL
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEDRALAIDGLLTCSAARRFALLRRWIALFGVTMPSREQLQRLWEEVALSREDAEPQLQL
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
GQYQFRRFRRRLYLLPLMADLREISLSWSLDDSLMLPDGLGELVSGEGDICLRAPQSQQK
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEECCCCHHHHHCCCCCEEEECCCCCCE
VSIRFSAQGKHRILGRAHSRPIKKLWQELGIPPWQRERIPLIYYDDQLIAALGIFVSEAG
EEEEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCC
QTPEGQQPWRLHWRKK
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA