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The map label for this gene is ligA

Identifier: 157371681

GI number: 157371681

Start: 3813527

End: 3815548

Strand: Reverse

Name: ligA

Synonym: Spro_3444

Alternate gene names: 157371681

Gene position: 3815548-3813527 (Counterclockwise)

Preceding gene: 157371682

Following gene: 157371680

Centisome position: 70.02

GC content: 58.95

Gene sequence:

>2022_bases
ATGGAATCGATAATCCAACAAATCAATCAACTACGAACCTCACTGCGCCATCACGAATATCAATACCATGTGCTGGACGC
GCCGGAAGTACCGGATGCGGAATACGACCGCCTGATGGGTGAACTGCGTGCCCTGGAAAGCGCACATCCGGAGCTGATCA
CCGCCGATTCTCCGACCCAGCGCGTGGGTGCCGCCCCGCTGGCGGCGTTCGATCAGGTGCGTCATGAAGTGCCTATGTTG
TCACTGGACAACGTTTTCGACGAAGAAAGCTTCCTGGCATTCTACAAACGAGTGCAGGATCGCCTGAAGAGCAGCGATCC
GCTGACCTTCTGCTGTGAGCTGAAGCTGGACGGTCTGGCGGTCAGCCTGCTGTACGAAGATGGCGAACTGGTTCGCGCGG
CTACCCGTGGCGACGGCACCACCGGTGAAAACATTACCTCTAACGTGCGCACCATCCGCGCCATTCCACTGCGATTGACA
GGTGACAATATCCCGCGCCGGCTGGAAGTGCGTGGCGAAGTGTTTATGCCGCAGGCCGGTTTCGAGCAGATGAACGAAGA
GGCGCGTCGCAAGGACGGCAAGATCTTCGCCAATCCGCGTAACGCTGCCGCCGGTTCGATTCGTCAGCTCGACCCGCGTA
TTACCGCCAAACGCCCACTGACCTTTTTCTGTTATGGCGTTGGCCTGCTGGAAGGCGGCGAATTGCCTCGCAGCCACTGG
CAGCGCCTGATGCAGTTCAAAGACTGGGGGTTACCGGTCAGCGATCGCGCCAAACGTTGTACCGGCAGCGACGAAGTGCT
GGCGTTCTACCGCCAGGTGGAGCAGGACCGCACGCAACTTGGGTTTGATATCGACGGCGTGGTAGTGAAAATCGACGATA
TTGACCTGCAGGAAACGCTGGGCTTTGTGGCGCGTGCGCCGCGCTGGGCGACGGCATTCAAATTCCCGGCGCAGGAGCAG
ATTACCCAGGTGCGCGAGGTGGAATTCCAGGTTGGCCGTACCGGTGCGATCACGCCGGTGGCAAGACTGGAGCCCGTGCT
GGTTGCCGGGGTGATCGTCAGCAACGCCACGCTGCATAACGCCGATGAAATTGAACGTTTGGGCCTGCGCATCGGCGATA
CGGTGATTGTGCGCCGTGCCGGCGACGTGATCCCGCAGGTGGTGGGAGTGATAGCCGAACGTCGTCCAGCGGACGCGCGT
GAAATTTTGTTCCCGCAGCATTGTCCGGTATGCGGTTCCGACGTTGAGAGAGTGGAAGGCGAAGCGGTGGCACGTTGCAC
CGGCGGGCTGATCTGCGCTGCACAACGCAAAGAAGCGCTGAAGCACTTTGTTTCCCGTCGGGCGTTGGACGTCGACGGCA
TGGGCGACAAAATCATCGAGCAGTTGGTGGAAAAAGAGTATGTGAAGAATCCGGCCGATCTGTTCCGTCTGTCCGCCGGC
ATTCTGACCGGGCTGGATCGCATGGGGCCGAAATCGGCGCAGAATCTGGTCAACGCGCTGGAAAAATCCAAGCAGACCAC
CTTTGCCCGTTTCCTGTATGCGCTTGGCATCCGTGAAGTCGGCGAAGCCACAGCGGCCAATCTGGCTGCACACTTCGGTA
CGCTGGATAAACTGTTAGCCGCTGATATCGAGGCGTTGAAAGAAGTGCCCGAGGTCGGTGAAATCGTGGCGAAGCACACG
CGTCATTTCCTTGACGAAGAGCTCAACCAACAGGTTATCCAGGAACTGGTTAGTGACGAAATCGGCATTAACTGGCCGGC
CCCGGTGGTGATAGTAGCGGAAGAGATCGACAGCCCGTTTGCCGGCAAAACCGTGGTGCTGACCGGCTCGTTGAGCCAGC
TATCACGTGACGAGGCCAAAGATCGTCTGACGGCGCTGGGCGCCAAGGTCAGTGGCAGCGTATCGAAGAAAACCGATCTG
GTGATTGCAGGTGAAGCCGCCGGTTCCAAACTGGTGAAGGCGCAGGAACTGGGCATTGAGGTCATTGACGAAGCGGAAAT
GATCCGCCTGCTGGGGGCCTGA

Upstream 100 bases:

>100_bases
AGTGTTGGAAAACAACGCCTGACCGACGGCCACGCGCACTGAACATCCGTAACAACAAAGCCCCCGCCTGCCGGGGGTTT
TTTATCTTTGATGGTGAGCC

Downstream 100 bases:

>100_bases
TGGAAAAAGAGAATCTGTTGGAAATTGCCAATACGGTAATGCCATTCGGCAAGTATCAGGGGCGGGTGCTGATTGACCTG
CCCGAAGAATACCTGTTGTG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MESIIQQINQLRTSLRHHEYQYHVLDAPEVPDAEYDRLMGELRALESAHPELITADSPTQRVGAAPLAAFDQVRHEVPML
SLDNVFDEESFLAFYKRVQDRLKSSDPLTFCCELKLDGLAVSLLYEDGELVRAATRGDGTTGENITSNVRTIRAIPLRLT
GDNIPRRLEVRGEVFMPQAGFEQMNEEARRKDGKIFANPRNAAAGSIRQLDPRITAKRPLTFFCYGVGLLEGGELPRSHW
QRLMQFKDWGLPVSDRAKRCTGSDEVLAFYRQVEQDRTQLGFDIDGVVVKIDDIDLQETLGFVARAPRWATAFKFPAQEQ
ITQVREVEFQVGRTGAITPVARLEPVLVAGVIVSNATLHNADEIERLGLRIGDTVIVRRAGDVIPQVVGVIAERRPADAR
EILFPQHCPVCGSDVERVEGEAVARCTGGLICAAQRKEALKHFVSRRALDVDGMGDKIIEQLVEKEYVKNPADLFRLSAG
ILTGLDRMGPKSAQNLVNALEKSKQTTFARFLYALGIREVGEATAANLAAHFGTLDKLLAADIEALKEVPEVGEIVAKHT
RHFLDEELNQQVIQELVSDEIGINWPAPVVIVAEEIDSPFAGKTVVLTGSLSQLSRDEAKDRLTALGAKVSGSVSKKTDL
VIAGEAAGSKLVKAQELGIEVIDEAEMIRLLGA

Sequences:

>Translated_673_residues
MESIIQQINQLRTSLRHHEYQYHVLDAPEVPDAEYDRLMGELRALESAHPELITADSPTQRVGAAPLAAFDQVRHEVPML
SLDNVFDEESFLAFYKRVQDRLKSSDPLTFCCELKLDGLAVSLLYEDGELVRAATRGDGTTGENITSNVRTIRAIPLRLT
GDNIPRRLEVRGEVFMPQAGFEQMNEEARRKDGKIFANPRNAAAGSIRQLDPRITAKRPLTFFCYGVGLLEGGELPRSHW
QRLMQFKDWGLPVSDRAKRCTGSDEVLAFYRQVEQDRTQLGFDIDGVVVKIDDIDLQETLGFVARAPRWATAFKFPAQEQ
ITQVREVEFQVGRTGAITPVARLEPVLVAGVIVSNATLHNADEIERLGLRIGDTVIVRRAGDVIPQVVGVIAERRPADAR
EILFPQHCPVCGSDVERVEGEAVARCTGGLICAAQRKEALKHFVSRRALDVDGMGDKIIEQLVEKEYVKNPADLFRLSAG
ILTGLDRMGPKSAQNLVNALEKSKQTTFARFLYALGIREVGEATAANLAAHFGTLDKLLAADIEALKEVPEVGEIVAKHT
RHFLDEELNQQVIQELVSDEIGINWPAPVVIVAEEIDSPFAGKTVVLTGSLSQLSRDEAKDRLTALGAKVSGSVSKKTDL
VIAGEAAGSKLVKAQELGIEVIDEAEMIRLLGA
>Mature_673_residues
MESIIQQINQLRTSLRHHEYQYHVLDAPEVPDAEYDRLMGELRALESAHPELITADSPTQRVGAAPLAAFDQVRHEVPML
SLDNVFDEESFLAFYKRVQDRLKSSDPLTFCCELKLDGLAVSLLYEDGELVRAATRGDGTTGENITSNVRTIRAIPLRLT
GDNIPRRLEVRGEVFMPQAGFEQMNEEARRKDGKIFANPRNAAAGSIRQLDPRITAKRPLTFFCYGVGLLEGGELPRSHW
QRLMQFKDWGLPVSDRAKRCTGSDEVLAFYRQVEQDRTQLGFDIDGVVVKIDDIDLQETLGFVARAPRWATAFKFPAQEQ
ITQVREVEFQVGRTGAITPVARLEPVLVAGVIVSNATLHNADEIERLGLRIGDTVIVRRAGDVIPQVVGVIAERRPADAR
EILFPQHCPVCGSDVERVEGEAVARCTGGLICAAQRKEALKHFVSRRALDVDGMGDKIIEQLVEKEYVKNPADLFRLSAG
ILTGLDRMGPKSAQNLVNALEKSKQTTFARFLYALGIREVGEATAANLAAHFGTLDKLLAADIEALKEVPEVGEIVAKHT
RHFLDEELNQQVIQELVSDEIGINWPAPVVIVAEEIDSPFAGKTVVLTGSLSQLSRDEAKDRLTALGAKVSGSVSKKTDL
VIAGEAAGSKLVKAQELGIEVIDEAEMIRLLGA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=673, Percent_Identity=79.4947994056464, Blast_Score=1109, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=571, Percent_Identity=23.292469352014, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_SERP5 (A8GHF2)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479670.1
- ProteinModelPortal:   A8GHF2
- STRING:   A8GHF2
- GeneID:   5604610
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3444
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   SPRO399741:SPRO_3444-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74141; Mature: 74141

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 173-173 BINDING 290-290 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIIQQINQLRTSLRHHEYQYHVLDAPEVPDAEYDRLMGELRALESAHPELITADSPTQ
CHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHH
RVGAAPLAAFDQVRHEVPMLSLDNVFDEESFLAFYKRVQDRLKSSDPLTFCCELKLDGLA
HHCCCHHHHHHHHHHHCCCEEHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEE
VSLLYEDGELVRAATRGDGTTGENITSNVRTIRAIPLRLTGDNIPRRLEVRGEVFMPQAG
EEEEECCCCEEEEECCCCCCCCCCHHHCCEEEEEEEEEECCCCCCCEEEECCCEECCCCC
FEQMNEEARRKDGKIFANPRNAAAGSIRQLDPRITAKRPLTFFCYGVGLLEGGELPRSHW
HHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCCCCCCCCEEEEEEHHHCCCCCCCHHHH
QRLMQFKDWGLPVSDRAKRCTGSDEVLAFYRQVEQDRTQLGFDIDGVVVKIDDIDLQETL
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHH
GFVARAPRWATAFKFPAQEQITQVREVEFQVGRTGAITPVARLEPVLVAGVIVSNATLHN
HHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHCCCCCC
ADEIERLGLRIGDTVIVRRAGDVIPQVVGVIAERRPADAREILFPQHCPVCGSDVERVEG
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHHCC
EAVARCTGGLICAAQRKEALKHFVSRRALDVDGMGDKIIEQLVEKEYVKNPADLFRLSAG
HHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
ILTGLDRMGPKSAQNLVNALEKSKQTTFARFLYALGIREVGEATAANLAAHFGTLDKLLA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADIEALKEVPEVGEIVAKHTRHFLDEELNQQVIQELVSDEIGINWPAPVVIVAEEIDSPF
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCC
AGKTVVLTGSLSQLSRDEAKDRLTALGAKVSGSVSKKTDLVIAGEAAGSKLVKAQELGIE
CCCEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEECCCCCHHHHHHHCCCH
VIDEAEMIRLLGA
HHCHHHHHHHHCC
>Mature Secondary Structure
MESIIQQINQLRTSLRHHEYQYHVLDAPEVPDAEYDRLMGELRALESAHPELITADSPTQ
CHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHH
RVGAAPLAAFDQVRHEVPMLSLDNVFDEESFLAFYKRVQDRLKSSDPLTFCCELKLDGLA
HHCCCHHHHHHHHHHHCCCEEHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEE
VSLLYEDGELVRAATRGDGTTGENITSNVRTIRAIPLRLTGDNIPRRLEVRGEVFMPQAG
EEEEECCCCEEEEECCCCCCCCCCHHHCCEEEEEEEEEECCCCCCCEEEECCCEECCCCC
FEQMNEEARRKDGKIFANPRNAAAGSIRQLDPRITAKRPLTFFCYGVGLLEGGELPRSHW
HHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCCCCCCCCEEEEEEHHHCCCCCCCHHHH
QRLMQFKDWGLPVSDRAKRCTGSDEVLAFYRQVEQDRTQLGFDIDGVVVKIDDIDLQETL
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHH
GFVARAPRWATAFKFPAQEQITQVREVEFQVGRTGAITPVARLEPVLVAGVIVSNATLHN
HHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHCCCCCC
ADEIERLGLRIGDTVIVRRAGDVIPQVVGVIAERRPADAREILFPQHCPVCGSDVERVEG
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHHCC
EAVARCTGGLICAAQRKEALKHFVSRRALDVDGMGDKIIEQLVEKEYVKNPADLFRLSAG
HHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
ILTGLDRMGPKSAQNLVNALEKSKQTTFARFLYALGIREVGEATAANLAAHFGTLDKLLA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADIEALKEVPEVGEIVAKHTRHFLDEELNQQVIQELVSDEIGINWPAPVVIVAEEIDSPF
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCC
AGKTVVLTGSLSQLSRDEAKDRLTALGAKVSGSVSKKTDLVIAGEAAGSKLVKAQELGIE
CCCEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEECCCCCHHHHHHHCCCH
VIDEAEMIRLLGA
HHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA