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The map label for this gene is purF [H]

Identifier: 157371565

GI number: 157371565

Start: 3683139

End: 3684656

Strand: Reverse

Name: purF [H]

Synonym: Spro_3328

Alternate gene names: 157371565

Gene position: 3684656-3683139 (Counterclockwise)

Preceding gene: 157371566

Following gene: 157371564

Centisome position: 67.62

GC content: 55.86

Gene sequence:

>1518_bases
ATGTGCGGTATTGTCGGTATCGCCGGTTTTATGCCGGTAAACCAGTCGATTTATGATGCGTTGATGGTGCTTCAGCACCG
TGGCCAGGATGCCGCAGGCATCGTCACCATAGATGCCCACAATGGGTTTCGTCTGCGTAAGGCAAACGGTCTGGTGAAGG
ATGTGTTTGAGGCACGCCATATGCAGCGCTTGCAGGGCAACATGGGTATTGGCCATGTGCGCTACCCAACGGCTGGCAGC
TCCAGTGCCTCAGAGGCTCAACCCTTTTATGTAAACTCGCCGTTCGGCTTGACGCTGGCACACAACGGCAACCTGACCAA
CGCCCATGAGCTGCGCCAAAAGCTGTTTGAAAGCGGCCGCCGCCATATCAACACCACCTCCGATTCCGAGATCCTGCTGA
ACGTACTGGCCAGTGAGCTGGATCGTTTCCAACATTATCCGCTGGAATCCGACAACATTTTCACCGCCGTTGCCGCCATG
CATCAGCAACTGCGTGGCGCTTACGCCTGCGTAGCGATGATCATCGGTCACGGTCTGCTGGCATTCCGTGACCCTAACGG
TATCCGCCCGTTGGTGATCGGCAAGCGTCAGCTTGAAGATGGCCGCAATGAATATATGGTGGCTTCCGAGAGCGTCGCAC
TGGACACGCTGGGCTTCGAATTCCTGCGCGACGTAGCGCCGGGCGAAGCGGTGTATATCACCGAGAAGGGCCAACTGTTC
ACTCGTCAGTGTGCAGAGAACCCGAAAACCAACCCGTGCCTGTTCGAATACGTCTATTTCGCTCGCCCCGACTCCTTTAT
GGACAAGATTTCGGTCTACAGCGCACGCGTGCGCATGGGGCAGAAGTTGGGTGAGAAGATTGCCCGCGAGTGGGAAGATT
TGGACATCGACGTGGTGATCCCAATCCCGGAAACGTCCTGTGATATCGCGTTGGAAATCGCGCGCATCCTGGATAAGCCA
TACCGTCAGGGCTTTGTGAAGAACCGCTATGTTGGCCGTACCTTTATTATGCCGGGCCAACAGGCGCGCCGTAAGTCGGT
ACGTCGTAAGTTGAACGCCAACCGTGCCGAGTTCCGCGACAAGAATGTGCTGTTGGTGGATGATTCCATCGTGCGCGGCA
CCACTTCAGAGCAGATCGTTGAGATGGCGCGTGACGCCGGGGCGAAACGAGTTTACCTGGCTTCCGCTGCGCCGGAAATT
CGCTTCCCTAACGTCTACGGCATCGACATGCCGAGTGCCAACGAACTGATCGCCCACGGCCGTGAAGTGGACGAAATTCG
CCAGATCATCGGTGCCGATGGTCTGATCTTCCAGGACCTGGACGATCTGATTGAAGCGGTACGCGAAGAGAACCCGGACA
TCACCCAGTTCGAATGCTCGGTATTCAACGGTATCTACGTCACCAAAGACGTCGACCAGAGCTACCTCGAATATCTGGAA
TCGCTGCGCAATGACGACGCCAAAGAAATACGTGGTCAGACCGAAGCCGAAAATCTGGAAATGCACAACGAAGGTTAA

Upstream 100 bases:

>100_bases
CAGTTACATCATCAGGTGGTTCTTTGACTACCTGCAGAGCACGTCGAGTTTCTTGCCGACCCATTTACCGGGGCGGTAGC
GCTGATGAGGAAAAGACAAC

Downstream 100 bases:

>100_bases
GTTTCATTCTTGACCAAAACAGTGCACCGCGGCGTTCGCTGCGGTGCATTATTCCTCCCTGACTTGCTTATGTAGCCCCA
TTGCGGCAAAGTCTGCCTGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MCGIVGIAGFMPVNQSIYDALMVLQHRGQDAAGIVTIDAHNGFRLRKANGLVKDVFEARHMQRLQGNMGIGHVRYPTAGS
SSASEAQPFYVNSPFGLTLAHNGNLTNAHELRQKLFESGRRHINTTSDSEILLNVLASELDRFQHYPLESDNIFTAVAAM
HQQLRGAYACVAMIIGHGLLAFRDPNGIRPLVIGKRQLEDGRNEYMVASESVALDTLGFEFLRDVAPGEAVYITEKGQLF
TRQCAENPKTNPCLFEYVYFARPDSFMDKISVYSARVRMGQKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILDKP
YRQGFVKNRYVGRTFIMPGQQARRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIVEMARDAGAKRVYLASAAPEI
RFPNVYGIDMPSANELIAHGREVDEIRQIIGADGLIFQDLDDLIEAVREENPDITQFECSVFNGIYVTKDVDQSYLEYLE
SLRNDDAKEIRGQTEAENLEMHNEG

Sequences:

>Translated_505_residues
MCGIVGIAGFMPVNQSIYDALMVLQHRGQDAAGIVTIDAHNGFRLRKANGLVKDVFEARHMQRLQGNMGIGHVRYPTAGS
SSASEAQPFYVNSPFGLTLAHNGNLTNAHELRQKLFESGRRHINTTSDSEILLNVLASELDRFQHYPLESDNIFTAVAAM
HQQLRGAYACVAMIIGHGLLAFRDPNGIRPLVIGKRQLEDGRNEYMVASESVALDTLGFEFLRDVAPGEAVYITEKGQLF
TRQCAENPKTNPCLFEYVYFARPDSFMDKISVYSARVRMGQKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILDKP
YRQGFVKNRYVGRTFIMPGQQARRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIVEMARDAGAKRVYLASAAPEI
RFPNVYGIDMPSANELIAHGREVDEIRQIIGADGLIFQDLDDLIEAVREENPDITQFECSVFNGIYVTKDVDQSYLEYLE
SLRNDDAKEIRGQTEAENLEMHNEG
>Mature_505_residues
MCGIVGIAGFMPVNQSIYDALMVLQHRGQDAAGIVTIDAHNGFRLRKANGLVKDVFEARHMQRLQGNMGIGHVRYPTAGS
SSASEAQPFYVNSPFGLTLAHNGNLTNAHELRQKLFESGRRHINTTSDSEILLNVLASELDRFQHYPLESDNIFTAVAAM
HQQLRGAYACVAMIIGHGLLAFRDPNGIRPLVIGKRQLEDGRNEYMVASESVALDTLGFEFLRDVAPGEAVYITEKGQLF
TRQCAENPKTNPCLFEYVYFARPDSFMDKISVYSARVRMGQKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILDKP
YRQGFVKNRYVGRTFIMPGQQARRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIVEMARDAGAKRVYLASAAPEI
RFPNVYGIDMPSANELIAHGREVDEIRQIIGADGLIFQDLDDLIEAVREENPDITQFECSVFNGIYVTKDVDQSYLEYLE
SLRNDDAKEIRGQTEAENLEMHNEG

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=473, Percent_Identity=36.5750528541226, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1788651, Length=505, Percent_Identity=87.5247524752475, Blast_Score=935, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=252, Percent_Identity=32.5396825396825, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=464, Percent_Identity=34.698275862069, Blast_Score=239, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6323958, Length=489, Percent_Identity=51.5337423312883, Blast_Score=498, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24659598, Length=466, Percent_Identity=35.8369098712446, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24659604, Length=459, Percent_Identity=35.9477124183007, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI28573187, Length=481, Percent_Identity=34.7193347193347, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56697; Mature: 56697

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIAGFMPVNQSIYDALMVLQHRGQDAAGIVTIDAHNGFRLRKANGLVKDVFEARH
CCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEHHCCHHHHHHHHHH
MQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPFGLTLAHNGNLTNAHELRQKLFESGR
HHHHHCCCCCCEEECCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHCCC
RHINTTSDSEILLNVLASELDRFQHYPLESDNIFTAVAAMHQQLRGAYACVAMIIGHGLL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
AFRDPNGIRPLVIGKRQLEDGRNEYMVASESVALDTLGFEFLRDVAPGEAVYITEKGQLF
EEECCCCCCCEEECHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
TRQCAENPKTNPCLFEYVYFARPDSFMDKISVYSARVRMGQKLGEKIAREWEDLDIDVVI
HHHHHCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
PIPETSCDIALEIARILDKPYRQGFVKNRYVGRTFIMPGQQARRKSVRRKLNANRAEFRD
ECCCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCC
KNVLLVDDSIVRGTTSEQIVEMARDAGAKRVYLASAAPEIRFPNVYGIDMPSANELIAHG
CCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
REVDEIRQIIGADGLIFQDLDDLIEAVREENPDITQFECSVFNGIYVTKDVDQSYLEYLE
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCHHHHHHHH
SLRNDDAKEIRGQTEAENLEMHNEG
HHCCCCHHHHCCCCCHHCCCCCCCC
>Mature Secondary Structure
MCGIVGIAGFMPVNQSIYDALMVLQHRGQDAAGIVTIDAHNGFRLRKANGLVKDVFEARH
CCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEHHCCHHHHHHHHHH
MQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPFGLTLAHNGNLTNAHELRQKLFESGR
HHHHHCCCCCCEEECCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHCCC
RHINTTSDSEILLNVLASELDRFQHYPLESDNIFTAVAAMHQQLRGAYACVAMIIGHGLL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
AFRDPNGIRPLVIGKRQLEDGRNEYMVASESVALDTLGFEFLRDVAPGEAVYITEKGQLF
EEECCCCCCCEEECHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
TRQCAENPKTNPCLFEYVYFARPDSFMDKISVYSARVRMGQKLGEKIAREWEDLDIDVVI
HHHHHCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
PIPETSCDIALEIARILDKPYRQGFVKNRYVGRTFIMPGQQARRKSVRRKLNANRAEFRD
ECCCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCC
KNVLLVDDSIVRGTTSEQIVEMARDAGAKRVYLASAAPEIRFPNVYGIDMPSANELIAHG
CCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
REVDEIRQIIGADGLIFQDLDDLIEAVREENPDITQFECSVFNGIYVTKDVDQSYLEYLE
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCHHHHHHHH
SLRNDDAKEIRGQTEAENLEMHNEG
HHCCCCHHHHCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]