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The map label for this gene is argT [H]

Identifier: 157371563

GI number: 157371563

Start: 3681352

End: 3682134

Strand: Reverse

Name: argT [H]

Synonym: Spro_3326

Alternate gene names: 157371563

Gene position: 3682134-3681352 (Counterclockwise)

Preceding gene: 157371564

Following gene: 157371562

Centisome position: 67.58

GC content: 53.77

Gene sequence:

>783_bases
ATGAAAAAGCTGGTTAAGGTTCTTCCTTTAGTCTTGGCTTTAAGCGCGGCAAGCAGCGCATTTGCCGCAGTACCATCAAC
GCTGAAGATCGGTACCGATCCGACCTATGCACCCTTTGAATCCAAAAATGCCAAGGGCGAGTTGGTCGGTTTTGATATCG
ATCTGGCCAAAGAGCTGTGCAAACGCATAAATACTAAATGTACCTTTGTGGAAAGCGATTTCGACGCGCTGATCCCGTCC
CTGAAGGCGAAGAAAATTGACGCCATCATCTCTTCTCTGTCGATCACCGAAAAACGCCAACAGGAAATTGCCTTCACCGA
CAAGCTGTATGCCGCCAACGCCCGCCTGATCGCGCCGAAAGGTTCCAAGCTGCTGCCTACCGTAGACGCGCTGAAAGGCA
AGAGCGTCGGCGTGCTGCAGGGGACTACCCAGGAAGCCTATGCCAATGCCATGTGGCAGCCTAAGGGCATCACCGTCGTG
CCTTATCAGAACCAGGATTTGGTGTATGCCGACCTGGCATCCGGCCGTATCGATGCGGCCTTCCAGGACGAAGTGGCGGG
CAGCGATGGCTTCCTGAAACAGCCTCCAGGCAAGGATTATGCATTTGCCGGTGAATCGGTGAAGGATGACAAATTCTTCG
GCGTGGGGACCGGCATGGGCCTGCGCAAGACCGATACCGAGCTGAAGGCGGCGCTGGATAAAGCCTTTGCCGAAATGCGC
AAAGACGGCACTTACGACAAGTATGCGAAGAAATATTTCGACTTTGACGTCTACGGCGGTTAA

Upstream 100 bases:

>100_bases
CCTCCGCCTACAGCAACAAATGTGGCACGATTACTGCAAATTGATTTGGTGGAAAAGTAATGCCCGCAGTGGCATCAAAA
AATCAATTTGAGGGTGATTT

Downstream 100 bases:

>100_bases
TGCCGCTTACCGTTCCATGTTGTGACTACGGGGCTGGCCCGCCGGCCCCGATGGTTCGGGATCCAATGACGACAGGGTGA
AGTAAATGCTGCAAGGCTAT

Product: cationic amino acid ABC transporter periplasmic binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKKLVKVLPLVLALSAASSAFAAVPSTLKIGTDPTYAPFESKNAKGELVGFDIDLAKELCKRINTKCTFVESDFDALIPS
LKAKKIDAIISSLSITEKRQQEIAFTDKLYAANARLIAPKGSKLLPTVDALKGKSVGVLQGTTQEAYANAMWQPKGITVV
PYQNQDLVYADLASGRIDAAFQDEVAGSDGFLKQPPGKDYAFAGESVKDDKFFGVGTGMGLRKTDTELKAALDKAFAEMR
KDGTYDKYAKKYFDFDVYGG

Sequences:

>Translated_260_residues
MKKLVKVLPLVLALSAASSAFAAVPSTLKIGTDPTYAPFESKNAKGELVGFDIDLAKELCKRINTKCTFVESDFDALIPS
LKAKKIDAIISSLSITEKRQQEIAFTDKLYAANARLIAPKGSKLLPTVDALKGKSVGVLQGTTQEAYANAMWQPKGITVV
PYQNQDLVYADLASGRIDAAFQDEVAGSDGFLKQPPGKDYAFAGESVKDDKFFGVGTGMGLRKTDTELKAALDKAFAEMR
KDGTYDKYAKKYFDFDVYGG
>Mature_260_residues
MKKLVKVLPLVLALSAASSAFAAVPSTLKIGTDPTYAPFESKNAKGELVGFDIDLAKELCKRINTKCTFVESDFDALIPS
LKAKKIDAIISSLSITEKRQQEIAFTDKLYAANARLIAPKGSKLLPTVDALKGKSVGVLQGTTQEAYANAMWQPKGITVV
PYQNQDLVYADLASGRIDAAFQDEVAGSDGFLKQPPGKDYAFAGESVKDDKFFGVGTGMGLRKTDTELKAALDKAFAEMR
KDGTYDKYAKKYFDFDVYGG

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=259, Percent_Identity=71.8146718146718, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=76.7932489451477, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787088, Length=253, Percent_Identity=38.7351778656126, Blast_Score=174, Evalue=4e-45,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=40.4081632653061, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI1788228, Length=232, Percent_Identity=32.7586206896552, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=29.6442687747036, Blast_Score=104, Evalue=5e-24,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28123; Mature: 28123

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVKVLPLVLALSAASSAFAAVPSTLKIGTDPTYAPFESKNAKGELVGFDIDLAKELC
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHH
KRINTKCTFVESDFDALIPSLKAKKIDAIISSLSITEKRQQEIAFTDKLYAANARLIAPK
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEECCC
GSKLLPTVDALKGKSVGVLQGTTQEAYANAMWQPKGITVVPYQNQDLVYADLASGRIDAA
CCCCCCHHHHHCCCCEEEEECCCHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCCCH
FQDEVAGSDGFLKQPPGKDYAFAGESVKDDKFFGVGTGMGLRKTDTELKAALDKAFAEMR
HHHHCCCCCCCCCCCCCCCCEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
KDGTYDKYAKKYFDFDVYGG
CCCCHHHHHHHHHCEEECCC
>Mature Secondary Structure
MKKLVKVLPLVLALSAASSAFAAVPSTLKIGTDPTYAPFESKNAKGELVGFDIDLAKELC
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHH
KRINTKCTFVESDFDALIPSLKAKKIDAIISSLSITEKRQQEIAFTDKLYAANARLIAPK
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEECCC
GSKLLPTVDALKGKSVGVLQGTTQEAYANAMWQPKGITVVPYQNQDLVYADLASGRIDAA
CCCCCCHHHHHCCCCEEEEECCCHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCCCH
FQDEVAGSDGFLKQPPGKDYAFAGESVKDDKFFGVGTGMGLRKTDTELKAALDKAFAEMR
HHHHCCCCCCCCCCCCCCCCEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
KDGTYDKYAKKYFDFDVYGG
CCCCHHHHHHHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]