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The map label for this gene is idnD [H]

Identifier: 157371524

GI number: 157371524

Start: 3638587

End: 3639621

Strand: Reverse

Name: idnD [H]

Synonym: Spro_3287

Alternate gene names: 157371524

Gene position: 3639621-3638587 (Counterclockwise)

Preceding gene: 157371525

Following gene: 157371523

Centisome position: 66.8

GC content: 56.23

Gene sequence:

>1035_bases
ATGATGAGAATTCAAACCCAATCCTGCGTGGTAAACGGCAAATATGACGTGGCGGTGGTGGAGCAGGAAGTGGATTATCA
GGGGATCGGTACGCTGGTGAAAATCACCCGTGGCGGCATCTGCGGTTCCGACCTGCATTATTACCAGGAAGGCAAAGTCG
GCAGTTTCCAGGTGCGTCAGCCGATGGTGCTCGGCCATGAGGTGATCGGTGAAGTGGTGGCCAGTGATACTGCCCGCCTG
CCGGTGGGTCAGAAGGTGGCGTTAAACCCCAGCAAACCCTGTGGGCAGTGCAAGTATTGTCTGGCAAGTCAGCAAAACCA
GTGTACTCAGATGCGTTTCTTCGGCAGCGCGATGTATTTCCCGCATGTTGACGGTGCCTTCACCCAGTACAAGGTGGTCG
ATAGCGCGCAGTGTATTGCCTTTGACGCCGATCGTGATGAAAAGGTGATGGTATTTGCCGAACCGCTGGCGGTGGCGATA
CACGCGGCGAAGCAGCCCGGCGAAGTGACAGGGAAAAAGGTGTTTGTTTCCGGCGTTGGCCCAATTGGCTGCCTGCTGGT
GGCGGCATTGAAGGCGCTGGGCGCCGGCGAAATTGTCTGTGCCGATCTCAGCCCACGTTGTCTGGATATTGCCCAAAAAA
TGGGGGCAACCCGTTGTCTGCATGCCGGCAATGACGACTTTAGCCTCTACCAGCAGGAAAAAGGGTACTTTGATATCGCT
TTTGAAGTCTCTGGCCATCCGTCGTCGTTGCAGCGCTGCCTGGAAGTCACCCGGGCCAAAGGCACGGTAGTACAGGTGGG
CATGGGGGGCAGCTTCCCGGATTTCCCGCTGATGTTGCTGATCGCCAAAGAGTTGAACCTGCTGGGCTCGTTCCGTTTTG
TTGAAGAATTTGATTTGGCGGTAGCCTGGTTGGCCGAGGGGACAGTCAATCCATTGCCGCTGCTGTCTGCCGAGTTTGAT
AACCAGCAACTGGCGCAGGCGTTGGCCTTTGCCGGTGACAAGAGTCAGGCGGCCAAAGTACAGTTGGTGTTCTGA

Upstream 100 bases:

>100_bases
TGTTCCTGTCGTCCCAGGCTTCCGATTTTGTTAATGGTCAGCTGCTGTTTGTCGATGGCGGCATGTCCGCGGCGGTATAG
CCGCTTGCCCAGGAGAAATC

Downstream 100 bases:

>100_bases
TTTTAGCCAATAGTCTGATGGCGAAGAGCGGCGATCCTTGTCTATAGTTAAGGACCGTATCATTTTTAATCACGGAGAAC
TTAACATGGCGAGAAATACG

Product: L-idonate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MMRIQTQSCVVNGKYDVAVVEQEVDYQGIGTLVKITRGGICGSDLHYYQEGKVGSFQVRQPMVLGHEVIGEVVASDTARL
PVGQKVALNPSKPCGQCKYCLASQQNQCTQMRFFGSAMYFPHVDGAFTQYKVVDSAQCIAFDADRDEKVMVFAEPLAVAI
HAAKQPGEVTGKKVFVSGVGPIGCLLVAALKALGAGEIVCADLSPRCLDIAQKMGATRCLHAGNDDFSLYQQEKGYFDIA
FEVSGHPSSLQRCLEVTRAKGTVVQVGMGGSFPDFPLMLLIAKELNLLGSFRFVEEFDLAVAWLAEGTVNPLPLLSAEFD
NQQLAQALAFAGDKSQAAKVQLVF

Sequences:

>Translated_344_residues
MMRIQTQSCVVNGKYDVAVVEQEVDYQGIGTLVKITRGGICGSDLHYYQEGKVGSFQVRQPMVLGHEVIGEVVASDTARL
PVGQKVALNPSKPCGQCKYCLASQQNQCTQMRFFGSAMYFPHVDGAFTQYKVVDSAQCIAFDADRDEKVMVFAEPLAVAI
HAAKQPGEVTGKKVFVSGVGPIGCLLVAALKALGAGEIVCADLSPRCLDIAQKMGATRCLHAGNDDFSLYQQEKGYFDIA
FEVSGHPSSLQRCLEVTRAKGTVVQVGMGGSFPDFPLMLLIAKELNLLGSFRFVEEFDLAVAWLAEGTVNPLPLLSAEFD
NQQLAQALAFAGDKSQAAKVQLVF
>Mature_344_residues
MMRIQTQSCVVNGKYDVAVVEQEVDYQGIGTLVKITRGGICGSDLHYYQEGKVGSFQVRQPMVLGHEVIGEVVASDTARL
PVGQKVALNPSKPCGQCKYCLASQQNQCTQMRFFGSAMYFPHVDGAFTQYKVVDSAQCIAFDADRDEKVMVFAEPLAVAI
HAAKQPGEVTGKKVFVSGVGPIGCLLVAALKALGAGEIVCADLSPRCLDIAQKMGATRCLHAGNDDFSLYQQEKGYFDIA
FEVSGHPSSLQRCLEVTRAKGTVVQVGMGGSFPDFPLMLLIAKELNLLGSFRFVEEFDLAVAWLAEGTVNPLPLLSAEFD
NQQLAQALAFAGDKSQAAKVQLVF

Specific function: Catalyzes the NADH/NADPH-dependent oxidation of L- idonate to 5-ketogluconate (5KG) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=329, Percent_Identity=30.6990881458967, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI4501933, Length=302, Percent_Identity=27.8145695364238, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4501929, Length=295, Percent_Identity=27.4576271186441, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI34577061, Length=302, Percent_Identity=27.1523178807947, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4501939, Length=264, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI71565152, Length=320, Percent_Identity=24.0625, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI156523966, Length=264, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790718, Length=343, Percent_Identity=66.4723032069971, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1787863, Length=346, Percent_Identity=27.4566473988439, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788073, Length=305, Percent_Identity=29.5081967213115, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1790045, Length=319, Percent_Identity=26.6457680250784, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788407, Length=336, Percent_Identity=27.9761904761905, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI226510992, Length=301, Percent_Identity=30.5647840531561, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786825, Length=202, Percent_Identity=30.6930693069307, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788075, Length=334, Percent_Identity=25.748502994012, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082125, Length=292, Percent_Identity=26.3698630136986, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786552, Length=263, Percent_Identity=26.615969581749, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=321, Percent_Identity=31.7757009345794, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17562878, Length=321, Percent_Identity=33.0218068535826, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6322619, Length=310, Percent_Identity=31.9354838709677, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319955, Length=310, Percent_Identity=31.9354838709677, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323099, Length=309, Percent_Identity=30.7443365695793, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6319258, Length=312, Percent_Identity=25.9615384615385, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319257, Length=312, Percent_Identity=25.9615384615385, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI17737897, Length=298, Percent_Identity=33.5570469798658, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI17137530, Length=298, Percent_Identity=34.5637583892617, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17737895, Length=297, Percent_Identity=25.9259259259259, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.264 [H]

Molecular weight: Translated: 37090; Mature: 37090

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRIQTQSCVVNGKYDVAVVEQEVDYQGIGTLVKITRGGICGSDLHYYQEGKVGSFQVRQ
CCEEECCCEEECCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCEEECC
PMVLGHEVIGEVVASDTARLPVGQKVALNPSKPCGQCKYCLASQQNQCTQMRFFGSAMYF
CHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHCEEEC
PHVDGAFTQYKVVDSAQCIAFDADRDEKVMVFAEPLAVAIHAAKQPGEVTGKKVFVSGVG
CCCCCCCEEEEEECCCEEEEEECCCCCEEEEEECCCEEEEECCCCCCCCCCCEEEEECCC
PIGCLLVAALKALGAGEIVCADLSPRCLDIAQKMGATRCLHAGNDDFSLYQQEKGYFDIA
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCEECCCCEEEEE
FEVSGHPSSLQRCLEVTRAKGTVVQVGMGGSFPDFPLMLLIAKELNLLGSFRFVEEFDLA
EEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAWLAEGTVNPLPLLSAEFDNQQLAQALAFAGDKSQAAKVQLVF
EEEECCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCEEEEEC
>Mature Secondary Structure
MMRIQTQSCVVNGKYDVAVVEQEVDYQGIGTLVKITRGGICGSDLHYYQEGKVGSFQVRQ
CCEEECCCEEECCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCEEECC
PMVLGHEVIGEVVASDTARLPVGQKVALNPSKPCGQCKYCLASQQNQCTQMRFFGSAMYF
CHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHCEEEC
PHVDGAFTQYKVVDSAQCIAFDADRDEKVMVFAEPLAVAIHAAKQPGEVTGKKVFVSGVG
CCCCCCCEEEEEECCCEEEEEECCCCCEEEEEECCCEEEEECCCCCCCCCCCEEEEECCC
PIGCLLVAALKALGAGEIVCADLSPRCLDIAQKMGATRCLHAGNDDFSLYQQEKGYFDIA
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCEECCCCEEEEE
FEVSGHPSSLQRCLEVTRAKGTVVQVGMGGSFPDFPLMLLIAKELNLLGSFRFVEEFDLA
EEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAWLAEGTVNPLPLLSAEFDNQQLAQALAFAGDKSQAAKVQLVF
EEEECCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 9658018 [H]