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The map label for this gene is menE [H]

Identifier: 157371516

GI number: 157371516

Start: 3622008

End: 3623393

Strand: Reverse

Name: menE [H]

Synonym: Spro_3279

Alternate gene names: 157371516

Gene position: 3623393-3622008 (Counterclockwise)

Preceding gene: 157371517

Following gene: 157371514

Centisome position: 66.5

GC content: 61.69

Gene sequence:

>1386_bases
ATGGCGCAGTTGAATGACTGGCCGTGGCGACATTGGGCGCAGCGGCGTCCGCAGGCGGCGGCGCTGGTTGTCGGCCATCA
GCCGGTGAGTTGGCTGGTGTTACGCCGTCAGGTGGATAAGCTGGCGGCGGATTTTCAACACCAGGGGGTGGAGCCAGGCT
GTGGTGTGGCGTTGTTCGGCAAGAACAGTTATCCCTTGTTGCTGGCCTATCTGGCGCTGTTGCAGTGCGGTGCGCGGCTG
CTGCCGCTGAATCCGGCGTTACCCGTTTCTCTGTTGGCGACGCTGTTGCCCGAGCTGGATATCGACTTTGCCTTCAGCCC
GGATCAACTGCCAGCCTTGCCGACCAGGATCATCCGTTTAAATCCCCCGTCTACCGAATCCCGCTGGCTTAATCCGCCGC
GCTGGGATCCTCAGCGGTTGGCGACCCTGACCCTGACTTCCGGCTCCAGCGGCAGACCGAAAGCGGCCGCACACAGTTAC
GCCGGCCATTTGGCCAGTGCCGAAGGCGTACTGCAGTTGATGGATTTCCAGGCGCAGGATCGCTGGTTGCTGTCGTTACC
CTTATTTCACGTTTCCGGACAAGGCATTGTCTGGCGCTGGTTGCTGGCAGGGGCCACGCTGATGGTCCGTGATATGCATC
CGTTGGCCGATGCTCTGGCGGGCTGTACTCATGCATCCTTGGTGCCGACTCAGCTCTGGCGCCTGCTGGCACAGCCGCTG
GCAGCCCTGACGCTGAAAAAAGTGCTGCTGGGGGGCGCGATGATCCCGGTAGAACTGACCAAACAGGCAGAGGCTACGGG
CATCCGCTGCTGGTGTGGCTATGGCCTGACTGAACTGGCTTCCACGGTGTGTGCCAAGCGTGCCGATGCACGGCCGGGCG
TTGGGCTGCCGCTGGCCGGTCGGGAAATTAAGCTGGTGAATGAAGAGGTGTGGATCCGCGGGCGGAGTCTGGCGCTGGGA
TACTGGCTACAGGGTTGCTTGCTGCCGATTGTCGATGATCAGGGCTGGTTCCATACCCGCGATCGTGGCGTCATGGAGCA
GGATGAGCTGCGGATTATCGGCCGGTTGGACAACCTGTTTTTCAGCGGCGGAGAAGGCGTTCAGCCAGAAGATATTGAGC
GCGTATTGGCGGCCCATCCGCAAATTTCACAGGTGGTGGTGGTGCCGGTGGATGACGCCGAGTTTGGTCAACGGCCGGTC
GCGGTGATCGACAGTGAGGAGTCTCTCTCGCTGGACGAGCTGCTGGCCTGGTCGCAGGACCGGCTGGTGAACTTCCAGCG
GCCAATGGCGCTGTTGGCGATGCCGAATGAGCTGAAGGCCGGGGGTATCAAGATCTCGCGCCGTCAGTTGCAGCAATGGG
CGGTGGAGCAGATCTCGGCTCAATAA

Upstream 100 bases:

>100_bases
TCCCTGGGCTAGACACGCTGGATTTGATGCAGGCGCAATTACTGCGACGCTGGCCGGATAGCCGGCTGCCGTTATTGGAG
ACGCAAGAGTTGGAGTGTTT

Downstream 100 bases:

>100_bases
AAATGGCCGCCTGAAGGCGGCCTTGAGGGTAACGCGGGTTATTTCAGACCTAACGCCTTGGCTACGCCGGCACCGTAATT
CGGGTGCACTTTGGTAAACA

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAQLNDWPWRHWAQRRPQAAALVVGHQPVSWLVLRRQVDKLAADFQHQGVEPGCGVALFGKNSYPLLLAYLALLQCGARL
LPLNPALPVSLLATLLPELDIDFAFSPDQLPALPTRIIRLNPPSTESRWLNPPRWDPQRLATLTLTSGSSGRPKAAAHSY
AGHLASAEGVLQLMDFQAQDRWLLSLPLFHVSGQGIVWRWLLAGATLMVRDMHPLADALAGCTHASLVPTQLWRLLAQPL
AALTLKKVLLGGAMIPVELTKQAEATGIRCWCGYGLTELASTVCAKRADARPGVGLPLAGREIKLVNEEVWIRGRSLALG
YWLQGCLLPIVDDQGWFHTRDRGVMEQDELRIIGRLDNLFFSGGEGVQPEDIERVLAAHPQISQVVVVPVDDAEFGQRPV
AVIDSEESLSLDELLAWSQDRLVNFQRPMALLAMPNELKAGGIKISRRQLQQWAVEQISAQ

Sequences:

>Translated_461_residues
MAQLNDWPWRHWAQRRPQAAALVVGHQPVSWLVLRRQVDKLAADFQHQGVEPGCGVALFGKNSYPLLLAYLALLQCGARL
LPLNPALPVSLLATLLPELDIDFAFSPDQLPALPTRIIRLNPPSTESRWLNPPRWDPQRLATLTLTSGSSGRPKAAAHSY
AGHLASAEGVLQLMDFQAQDRWLLSLPLFHVSGQGIVWRWLLAGATLMVRDMHPLADALAGCTHASLVPTQLWRLLAQPL
AALTLKKVLLGGAMIPVELTKQAEATGIRCWCGYGLTELASTVCAKRADARPGVGLPLAGREIKLVNEEVWIRGRSLALG
YWLQGCLLPIVDDQGWFHTRDRGVMEQDELRIIGRLDNLFFSGGEGVQPEDIERVLAAHPQISQVVVVPVDDAEFGQRPV
AVIDSEESLSLDELLAWSQDRLVNFQRPMALLAMPNELKAGGIKISRRQLQQWAVEQISAQ
>Mature_460_residues
AQLNDWPWRHWAQRRPQAAALVVGHQPVSWLVLRRQVDKLAADFQHQGVEPGCGVALFGKNSYPLLLAYLALLQCGARLL
PLNPALPVSLLATLLPELDIDFAFSPDQLPALPTRIIRLNPPSTESRWLNPPRWDPQRLATLTLTSGSSGRPKAAAHSYA
GHLASAEGVLQLMDFQAQDRWLLSLPLFHVSGQGIVWRWLLAGATLMVRDMHPLADALAGCTHASLVPTQLWRLLAQPLA
ALTLKKVLLGGAMIPVELTKQAEATGIRCWCGYGLTELASTVCAKRADARPGVGLPLAGREIKLVNEEVWIRGRSLALGY
WLQGCLLPIVDDQGWFHTRDRGVMEQDELRIIGRLDNLFFSGGEGVQPEDIERVLAAHPQISQVVVVPVDDAEFGQRPVA
VIDSEESLSLDELLAWSQDRLVNFQRPMALLAMPNELKAGGIKISRRQLQQWAVEQISAQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=532, Percent_Identity=24.2481203007519, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1788595, Length=458, Percent_Identity=57.4235807860262, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI145693145, Length=527, Percent_Identity=24.4781783681214, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788107, Length=354, Percent_Identity=27.683615819209, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI221142682, Length=484, Percent_Identity=23.3471074380165, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1789201, Length=320, Percent_Identity=28.125, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1786801, Length=437, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32563687, Length=398, Percent_Identity=24.1206030150754, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=405, Percent_Identity=26.1728395061728, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=391, Percent_Identity=23.7851662404092, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=356, Percent_Identity=25.2808988764045, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=362, Percent_Identity=24.0331491712707, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71994703, Length=339, Percent_Identity=24.4837758112094, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994694, Length=337, Percent_Identity=24.3323442136499, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994690, Length=337, Percent_Identity=24.3323442136499, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17557194, Length=364, Percent_Identity=24.4505494505494, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538037, Length=359, Percent_Identity=24.2339832869081, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=343, Percent_Identity=23.0320699708455, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24581924, Length=518, Percent_Identity=23.3590733590734, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50849; Mature: 50717

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLNDWPWRHWAQRRPQAAALVVGHQPVSWLVLRRQVDKLAADFQHQGVEPGCGVALFG
CCCCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
KNSYPLLLAYLALLQCGARLLPLNPALPVSLLATLLPELDIDFAFSPDQLPALPTRIIRL
CCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
NPPSTESRWLNPPRWDPQRLATLTLTSGSSGRPKAAAHSYAGHLASAEGVLQLMDFQAQD
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
RWLLSLPLFHVSGQGIVWRWLLAGATLMVRDMHPLADALAGCTHASLVPTQLWRLLAQPL
CEEEEECEEEECCCCHHHHHHHHCCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AALTLKKVLLGGAMIPVELTKQAEATGIRCWCGYGLTELASTVCAKRADARPGVGLPLAG
HHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCC
REIKLVNEEVWIRGRSLALGYWLQGCLLPIVDDQGWFHTRDRGVMEQDELRIIGRLDNLF
CEEEEECHHHEEECCCEEHHHHHHCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHE
FSGGEGVQPEDIERVLAAHPQISQVVVVPVDDAEFGQRPVAVIDSEESLSLDELLAWSQD
ECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCEEEECCCCCCCHHHHHHCCHH
RLVNFQRPMALLAMPNELKAGGIKISRRQLQQWAVEQISAQ
HHHCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AQLNDWPWRHWAQRRPQAAALVVGHQPVSWLVLRRQVDKLAADFQHQGVEPGCGVALFG
CCCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
KNSYPLLLAYLALLQCGARLLPLNPALPVSLLATLLPELDIDFAFSPDQLPALPTRIIRL
CCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
NPPSTESRWLNPPRWDPQRLATLTLTSGSSGRPKAAAHSYAGHLASAEGVLQLMDFQAQD
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
RWLLSLPLFHVSGQGIVWRWLLAGATLMVRDMHPLADALAGCTHASLVPTQLWRLLAQPL
CEEEEECEEEECCCCHHHHHHHHCCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AALTLKKVLLGGAMIPVELTKQAEATGIRCWCGYGLTELASTVCAKRADARPGVGLPLAG
HHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCC
REIKLVNEEVWIRGRSLALGYWLQGCLLPIVDDQGWFHTRDRGVMEQDELRIIGRLDNLF
CEEEEECHHHEEECCCEEHHHHHHCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHE
FSGGEGVQPEDIERVLAAHPQISQVVVVPVDDAEFGQRPVAVIDSEESLSLDELLAWSQD
ECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCEEEECCCCCCCHHHHHHCCHH
RLVNFQRPMALLAMPNELKAGGIKISRRQLQQWAVEQISAQ
HHHCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]