Click here to switch to the map view.

The map label for this gene is 157371496

Identifier: 157371496

GI number: 157371496

Start: 3595455

End: 3596351

Strand: Reverse

Name: 157371496

Synonym: Spro_3259

Alternate gene names: NA

Gene position: 3596351-3595455 (Counterclockwise)

Preceding gene: 157371498

Following gene: 157371495

Centisome position: 66.0

GC content: 54.74

Gene sequence:

>897_bases
ATGAAAAAAAACCTTGGTTATCTGACGGCTATGACGGTTGCTCTGTGTTTCGGCGCTCAGGCGCAGCTTAATCCGGCACA
GCCGTTGTCCAGTGCGCCGCCCTATTCGCTTTTTGAACAGTGGGCGCAGCCGGTCGCGCCATTTCAGATGTTCCCGCATG
TTTATTACGTGGGCACCCGCAACCTATCGTCCGTGCTGCTGAGCACGCCGGAAGGACTGATTCTGATCGACGCCGCGCTG
GACGCCAGCGCTCCGGCAATAAGAGCGCACATAGAGGCTTTGGGTTTTAACATCAAGGATCTGCGCTATATCCTCAATAG
TCACGCGCGTCTCGATCAGGCGGGTGGCATTGCGCGTCTGCAAGTCTGGAGCGGGGCAAAAGTTGTCGCCAGCGCTGCCA
ATGCCCAACAGTTGGCGCTTGGCGGCAAACAGGATTTTGCTCTGGGAGATGCGCTGAGCTTCCCGCCGGTGAAGGTCGAT
ATTATCGTCGGGGAGGGGGACAGCATTACCTTGGGCGACCTGAAATTAACGGCATTGATGACGCCAGGCCATTTGCCGGG
GGCCACCTCGTGGCTGACCACGCTACATCAGGGAGGGCAAAGTTACCGACTGGTCTATGCTGATAGCCTGGCAACGCCGG
ACTACTATCTGATCGACAACAAAAACTACCCCAGTCTGGTGCAGGATATCCGTGGCAGTTTTGCTCGGTTGGCTCAGCAG
CAGGCGGATATTTTTATCGTCAATAAGGGAACGCGCTTCGATCTGGATAATAAAATGCAACGCCTGCAGGCTGGCGATCT
GGATGCGTTTGTCGATCGTCAGGGGTTGCAGCAATATGTGCAACAGTCACAACAGACATTTGAGGCGCAGTTAAAACAGC
AACAAAGTAAAATGTGA

Upstream 100 bases:

>100_bases
GTCCATTTGCAGGATGTTCATTAATGGCCTTCTTAATTGAATACGTTTCAATATTGGGCTGAGTATACTTCAGCCCCGTT
TACCTCCATAGAATTGGAAC

Downstream 100 bases:

>100_bases
ATTGCGCTGGTTGGCTACCGTGGCCTGCTGAGGATATTGGCGCGAACCGCACCTATCGACAAAGTGAAATTTGTAGAAAA
GCCTTCTCTCTTCTAGACTT

Product: beta-lactamase

Products: NA

Alternate protein names: Beta-lactamase type II; Penicillinase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKKNLGYLTAMTVALCFGAQAQLNPAQPLSSAPPYSLFEQWAQPVAPFQMFPHVYYVGTRNLSSVLLSTPEGLILIDAAL
DASAPAIRAHIEALGFNIKDLRYILNSHARLDQAGGIARLQVWSGAKVVASAANAQQLALGGKQDFALGDALSFPPVKVD
IIVGEGDSITLGDLKLTALMTPGHLPGATSWLTTLHQGGQSYRLVYADSLATPDYYLIDNKNYPSLVQDIRGSFARLAQQ
QADIFIVNKGTRFDLDNKMQRLQAGDLDAFVDRQGLQQYVQQSQQTFEAQLKQQQSKM

Sequences:

>Translated_298_residues
MKKNLGYLTAMTVALCFGAQAQLNPAQPLSSAPPYSLFEQWAQPVAPFQMFPHVYYVGTRNLSSVLLSTPEGLILIDAAL
DASAPAIRAHIEALGFNIKDLRYILNSHARLDQAGGIARLQVWSGAKVVASAANAQQLALGGKQDFALGDALSFPPVKVD
IIVGEGDSITLGDLKLTALMTPGHLPGATSWLTTLHQGGQSYRLVYADSLATPDYYLIDNKNYPSLVQDIRGSFARLAQQ
QADIFIVNKGTRFDLDNKMQRLQAGDLDAFVDRQGLQQYVQQSQQTFEAQLKQQQSKM
>Mature_298_residues
MKKNLGYLTAMTVALCFGAQAQLNPAQPLSSAPPYSLFEQWAQPVAPFQMFPHVYYVGTRNLSSVLLSTPEGLILIDAAL
DASAPAIRAHIEALGFNIKDLRYILNSHARLDQAGGIARLQVWSGAKVVASAANAQQLALGGKQDFALGDALSFPPVKVD
IIVGEGDSITLGDLKLTALMTPGHLPGATSWLTTLHQGGQSYRLVYADSLATPDYYLIDNKNYPSLVQDIRGSFARLAQQ
QADIFIVNKGTRFDLDNKMQRLQAGDLDAFVDRQGLQQYVQQSQQTFEAQLKQQQSKM

Specific function: Has a high activity against imipenem [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-B beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001018
- InterPro:   IPR001279 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 32497; Mature: 32497

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNLGYLTAMTVALCFGAQAQLNPAQPLSSAPPYSLFEQWAQPVAPFQMFPHVYYVGTR
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCEEEEEECC
NLSSVLLSTPEGLILIDAALDASAPAIRAHIEALGFNIKDLRYILNSHARLDQAGGIARL
CHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCEEEE
QVWSGAKVVASAANAQQLALGGKQDFALGDALSFPPVKVDIIVGEGDSITLGDLKLTALM
EEECCCHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEECCEEEEEEE
TPGHLPGATSWLTTLHQGGQSYRLVYADSLATPDYYLIDNKNYPSLVQDIRGSFARLAQQ
CCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
QADIFIVNKGTRFDLDNKMQRLQAGDLDAFVDRQGLQQYVQQSQQTFEAQLKQQQSKM
CCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKNLGYLTAMTVALCFGAQAQLNPAQPLSSAPPYSLFEQWAQPVAPFQMFPHVYYVGTR
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCEEEEEECC
NLSSVLLSTPEGLILIDAALDASAPAIRAHIEALGFNIKDLRYILNSHARLDQAGGIARL
CHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCEEEE
QVWSGAKVVASAANAQQLALGGKQDFALGDALSFPPVKVDIIVGEGDSITLGDLKLTALM
EEECCCHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEECCEEEEEEE
TPGHLPGATSWLTTLHQGGQSYRLVYADSLATPDYYLIDNKNYPSLVQDIRGSFARLAQQ
CCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
QADIFIVNKGTRFDLDNKMQRLQAGDLDAFVDRQGLQQYVQQSQQTFEAQLKQQQSKM
CCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8018721; 3931629; 9811546 [H]