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The map label for this gene is amiD [H]

Identifier: 157371447

GI number: 157371447

Start: 3535513

End: 3536280

Strand: Reverse

Name: amiD [H]

Synonym: Spro_3210

Alternate gene names: 157371447

Gene position: 3536280-3535513 (Counterclockwise)

Preceding gene: 157371449

Following gene: 157371446

Centisome position: 64.9

GC content: 56.12

Gene sequence:

>768_bases
ATGTTCAGTATGGATTACAACAGCTACCGTTCGGTAGCCAGCTTTGGCCACCGGGTGCGTTTTCTGGTGCTGCATTACAC
GGCGCAAAACTTTGCCGATTCGGTGCAGTCACTGACCGGTAAGTCGGTCAGCGCCCATTATCTGGTGCCGGATCCTGAAG
ATCAAAGCTATCAGGCCGCCGGTTTCAACGGCGTGCGCATTTTCAACCTGGTCGATGAAGTGGAACGCGCCTGGCATGCC
GGGGCCAGTCAATGGGGCAATCGCAATAATCTTAACGATACGTCCATTGGCATTGAGATCGTCAATCTGGCCAGCGGCGA
TGGCGAAGATATTACCTTCCCGCCGTTTAACCCGCAGCAGATTGCCGCGGTCAGCCAACTGGCGCAGAACATTCTGCAAC
GCTATCCGGACATCACGCCGGTCAACGTGGTGGCGCATTCCGATATTGCTCCGGGCCGCAAAAGTGATCCAGGCCCGAAG
TTTCCGTGGCGCCAGCTATATCTGGCAGGTGTAGGCGCCTGGTATGACGAAGCGGTGAAGCATCGTCACCAGCATGAATA
TCAGTGCCAGGGCCTGCCGGCGCAGCCTGATTTGCTGGCGCTGTTCGCTAAATACGGTTACGACACCTCGGCAGCGACCA
ATGCCGAAGGTTATCGCCAACTGGTGCGGGCCTTCCAGCTGCATTTCCGTCAACGAAAGTATGACGGAGTGATGGATGTG
GAAACCGCCGCCATTTTGCGTGCGCTGGTGGATAAATATGCCGCTTAA

Upstream 100 bases:

>100_bases
TCCGCAAAGACATTGTAGGGTCGCTGTACGTTGCGGCCGTTTGAGCGGAACGGATCCTGCAATGCTGTGGCACGCGAGTT
TAACCAACAAGGAATCTATT

Downstream 100 bases:

>100_bases
CGTCTGACTGCGGATAGGGGACGCCTTATCCGCCTTTTTCAGCCGGCCTTCCTCCGCTAAACTGATATCCTTTCTCGCCA
CCGATAACGGGATGCATTGT

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MFSMDYNSYRSVASFGHRVRFLVLHYTAQNFADSVQSLTGKSVSAHYLVPDPEDQSYQAAGFNGVRIFNLVDEVERAWHA
GASQWGNRNNLNDTSIGIEIVNLASGDGEDITFPPFNPQQIAAVSQLAQNILQRYPDITPVNVVAHSDIAPGRKSDPGPK
FPWRQLYLAGVGAWYDEAVKHRHQHEYQCQGLPAQPDLLALFAKYGYDTSAATNAEGYRQLVRAFQLHFRQRKYDGVMDV
ETAAILRALVDKYAA

Sequences:

>Translated_255_residues
MFSMDYNSYRSVASFGHRVRFLVLHYTAQNFADSVQSLTGKSVSAHYLVPDPEDQSYQAAGFNGVRIFNLVDEVERAWHA
GASQWGNRNNLNDTSIGIEIVNLASGDGEDITFPPFNPQQIAAVSQLAQNILQRYPDITPVNVVAHSDIAPGRKSDPGPK
FPWRQLYLAGVGAWYDEAVKHRHQHEYQCQGLPAQPDLLALFAKYGYDTSAATNAEGYRQLVRAFQLHFRQRKYDGVMDV
ETAAILRALVDKYAA
>Mature_255_residues
MFSMDYNSYRSVASFGHRVRFLVLHYTAQNFADSVQSLTGKSVSAHYLVPDPEDQSYQAAGFNGVRIFNLVDEVERAWHA
GASQWGNRNNLNDTSIGIEIVNLASGDGEDITFPPFNPQQIAAVSQLAQNILQRYPDITPVNVVAHSDIAPGRKSDPGPK
FPWRQLYLAGVGAWYDEAVKHRHQHEYQCQGLPAQPDLLALFAKYGYDTSAATNAEGYRQLVRAFQLHFRQRKYDGVMDV
ETAAILRALVDKYAA

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=242, Percent_Identity=46.2809917355372, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1786300, Length=89, Percent_Identity=44.9438202247191, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 28524; Mature: 28524

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSMDYNSYRSVASFGHRVRFLVLHYTAQNFADSVQSLTGKSVSAHYLVPDPEDQSYQAA
CCCCCHHHHHHHHHHHHHHEEHEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECC
GFNGVRIFNLVDEVERAWHAGASQWGNRNNLNDTSIGIEIVNLASGDGEDITFPPFNPQQ
CCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHH
IAAVSQLAQNILQRYPDITPVNVVAHSDIAPGRKSDPGPKFPWRQLYLAGVGAWYDEAVK
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HRHQHEYQCQGLPAQPDLLALFAKYGYDTSAATNAEGYRQLVRAFQLHFRQRKYDGVMDV
HHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
ETAAILRALVDKYAA
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MFSMDYNSYRSVASFGHRVRFLVLHYTAQNFADSVQSLTGKSVSAHYLVPDPEDQSYQAA
CCCCCHHHHHHHHHHHHHHEEHEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECC
GFNGVRIFNLVDEVERAWHAGASQWGNRNNLNDTSIGIEIVNLASGDGEDITFPPFNPQQ
CCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHH
IAAVSQLAQNILQRYPDITPVNVVAHSDIAPGRKSDPGPKFPWRQLYLAGVGAWYDEAVK
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HRHQHEYQCQGLPAQPDLLALFAKYGYDTSAATNAEGYRQLVRAFQLHFRQRKYDGVMDV
HHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
ETAAILRALVDKYAA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]