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The map label for this gene is livJ [C]

Identifier: 157371440

GI number: 157371440

Start: 3527012

End: 3528130

Strand: Reverse

Name: livJ [C]

Synonym: Spro_3203

Alternate gene names: 157371440

Gene position: 3528130-3527012 (Counterclockwise)

Preceding gene: 157371442

Following gene: 157371439

Centisome position: 64.75

GC content: 57.73

Gene sequence:

>1119_bases
ATGTCATCGAAATTGATTAAAAGTCCGCTCGCTGCTCTGCTTATCGGTTGTTTAGGTTCTGCTTTTTATGCTCAGGCGGA
TATTGTTATTGGCGTCGCCGGGCCGTTCTCCGGGCCGAATGCGACCTATGGCGATCAGTACTGGCGTGGTGCCTCGCAGG
CGGCGGCGGATATCAACGCCGCCGGCGGGATTAAAGGGGAAAAAATCAAACTGGTGCAGGGGGATGACGCCTGCGAACCC
AAACAGGCGGTTTCGGTCGCCAACCGGTTAGTGGATCAGGACAAGGTCGCGGCGGTGGTCGGGCACTTCTGCTCCTCCTC
CACCATGCCGGCCTCCGAGGTGTATGACGAAGCCGGTATCATCGCCATCACCCCCGGATCCACCAACCCGCAGATTACCG
AACGCGGCATGACCAACATGTTCCGCATGTGCGGCCGTGATGACCAGCAAGGGGTGATCGCCACCAACTACATGCTCAAT
ACCCTGAAAGCCAAACGCATTGCGGTGATCCACGACAAAGACACCTACGGTCAGGGGCTGGCAGATGCCGCCAGGGCCGA
GATGAACAAGCGTGGCGTGAAGGAAGTGCTGTATGAAGGGCTGTCACGCGGTGAAAAAGACTTCAACGCGCTGGTGACCA
AGATCGCCTCGGTCAAACCCGACGTGGTCTATTTCGGTGGCTGCCATCCAGAGGCTGGGCCATTGGTGCGCCAGATGCGT
GAGCAGGGTGTGACCGCCAAGTTCTTCTCCGGTGACTGCATCGTGACCGAAGAGCTGGTGACCGCTGCCGGTGGGCCGCA
GTACACCAACGGCGTGCTGATGACCTTCGGTAACGATCCACGTCAAATTCCTGAGGGCAAGGCGGTGATCGCCAAGTTCC
GCGCCAGCGGTTTTGAGCCGGAAGGCTACACCCTCTACTCCTACGCTTCGATTCAGGCAATTGCCGCAGCCTTCAGCGCT
ACCGGCGGCAAAGACAGCGCCAAGGCCAGCGAATGGCTGAAATCGCATGATGTGGATACCGTGATGGGCAAAAAGGCCTG
GGACAAGAAAGGCGACCTGAAGGTATCGGATTACGTGGTCTATCAATGGGACGATAAAGGCAAATATCACCAACTCTAA

Upstream 100 bases:

>100_bases
GCGACTGCGCTCTCGCGCAAATTATTTTCCCCGTCTACACATAAAGTGACTGCTCCACGCCCCTGGGCGTTTCGCTAAAA
CAGTGCTGAGGAATACAACA

Downstream 100 bases:

>100_bases
TCAGCCCGAAGGCTCAGGTTTATCCCGCCCACAGGGTGCTGGATTGACATGACTGACAAGCGGATCCGCGTGTTCGGCGG
ATCCGCGCCGATACTTCCCT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSSKLIKSPLAALLIGCLGSAFYAQADIVIGVAGPFSGPNATYGDQYWRGASQAAADINAAGGIKGEKIKLVQGDDACEP
KQAVSVANRLVDQDKVAAVVGHFCSSSTMPASEVYDEAGIIAITPGSTNPQITERGMTNMFRMCGRDDQQGVIATNYMLN
TLKAKRIAVIHDKDTYGQGLADAARAEMNKRGVKEVLYEGLSRGEKDFNALVTKIASVKPDVVYFGGCHPEAGPLVRQMR
EQGVTAKFFSGDCIVTEELVTAAGGPQYTNGVLMTFGNDPRQIPEGKAVIAKFRASGFEPEGYTLYSYASIQAIAAAFSA
TGGKDSAKASEWLKSHDVDTVMGKKAWDKKGDLKVSDYVVYQWDDKGKYHQL

Sequences:

>Translated_372_residues
MSSKLIKSPLAALLIGCLGSAFYAQADIVIGVAGPFSGPNATYGDQYWRGASQAAADINAAGGIKGEKIKLVQGDDACEP
KQAVSVANRLVDQDKVAAVVGHFCSSSTMPASEVYDEAGIIAITPGSTNPQITERGMTNMFRMCGRDDQQGVIATNYMLN
TLKAKRIAVIHDKDTYGQGLADAARAEMNKRGVKEVLYEGLSRGEKDFNALVTKIASVKPDVVYFGGCHPEAGPLVRQMR
EQGVTAKFFSGDCIVTEELVTAAGGPQYTNGVLMTFGNDPRQIPEGKAVIAKFRASGFEPEGYTLYSYASIQAIAAAFSA
TGGKDSAKASEWLKSHDVDTVMGKKAWDKKGDLKVSDYVVYQWDDKGKYHQL
>Mature_371_residues
SSKLIKSPLAALLIGCLGSAFYAQADIVIGVAGPFSGPNATYGDQYWRGASQAAADINAAGGIKGEKIKLVQGDDACEPK
QAVSVANRLVDQDKVAAVVGHFCSSSTMPASEVYDEAGIIAITPGSTNPQITERGMTNMFRMCGRDDQQGVIATNYMLNT
LKAKRIAVIHDKDTYGQGLADAARAEMNKRGVKEVLYEGLSRGEKDFNALVTKIASVKPDVVYFGGCHPEAGPLVRQMRE
QGVTAKFFSGDCIVTEELVTAAGGPQYTNGVLMTFGNDPRQIPEGKAVIAKFRASGFEPEGYTLYSYASIQAIAAAFSAT
GGKDSAKASEWLKSHDVDTVMGKKAWDKKGDLKVSDYVVYQWDDKGKYHQL

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=360, Percent_Identity=42.5, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1789867, Length=342, Percent_Identity=41.5204678362573, Blast_Score=278, Evalue=5e-76,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39796; Mature: 39665

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLIKSPLAALLIGCLGSAFYAQADIVIGVAGPFSGPNATYGDQYWRGASQAAADINA
CCCHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCCCCCCHHHHCCHHHHHHCCCC
AGGIKGEKIKLVQGDDACEPKQAVSVANRLVDQDKVAAVVGHFCSSSTMPASEVYDEAGI
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCE
IAITPGSTNPQITERGMTNMFRMCGRDDQQGVIATNYMLNTLKAKRIAVIHDKDTYGQGL
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCEEEEEECCCCCCCCH
ADAARAEMNKRGVKEVLYEGLSRGEKDFNALVTKIASVKPDVVYFGGCHPEAGPLVRQMR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
EQGVTAKFFSGDCIVTEELVTAAGGPQYTNGVLMTFGNDPRQIPEGKAVIAKFRASGFEP
HCCCEEEEECCCEEEHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCC
EGYTLYSYASIQAIAAAFSATGGKDSAKASEWLKSHDVDTVMGKKAWDKKGDLKVSDYVV
CCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEEEEEE
YQWDDKGKYHQL
EEECCCCCCCCC
>Mature Secondary Structure 
SSKLIKSPLAALLIGCLGSAFYAQADIVIGVAGPFSGPNATYGDQYWRGASQAAADINA
CCHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCCCCCCHHHHCCHHHHHHCCCC
AGGIKGEKIKLVQGDDACEPKQAVSVANRLVDQDKVAAVVGHFCSSSTMPASEVYDEAGI
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCE
IAITPGSTNPQITERGMTNMFRMCGRDDQQGVIATNYMLNTLKAKRIAVIHDKDTYGQGL
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCEEEEEECCCCCCCCH
ADAARAEMNKRGVKEVLYEGLSRGEKDFNALVTKIASVKPDVVYFGGCHPEAGPLVRQMR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
EQGVTAKFFSGDCIVTEELVTAAGGPQYTNGVLMTFGNDPRQIPEGKAVIAKFRASGFEP
HCCCEEEEECCCEEEHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCC
EGYTLYSYASIQAIAAAFSATGGKDSAKASEWLKSHDVDTVMGKKAWDKKGDLKVSDYVV
CCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEEEEEE
YQWDDKGKYHQL
EEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA