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The map label for this gene is citF [H]

Identifier: 157371407

GI number: 157371407

Start: 3486310

End: 3487815

Strand: Reverse

Name: citF [H]

Synonym: Spro_3168

Alternate gene names: 157371407

Gene position: 3487815-3486310 (Counterclockwise)

Preceding gene: 157371408

Following gene: 157371406

Centisome position: 64.01

GC content: 62.22

Gene sequence:

>1506_bases
ATGAAACGCCAACAACGACTGATGACCCTGAATAATTTGGCGGAGTTGCGCTGCTATCAGGCGTCCTCCAAGGCTAACCA
GCAGGCGAGCAAACCCCGTGATATCAAACGCTGCGACACGCTGGAACAGGCGGTGCGCCGCAGCGGGTTGCAGGATGGCA
TGACCATTTCTTTCCACCATGCGTTTCGCGGTGGCGATCTGGCGCTAAATCAGGTGATGGAAACGCTGGCGGCGATGGGG
TTCCGTAACCTGACGCTGGCTTCCAGCTCGCTGAGCGAATGCCATGCGCCGCTGGTCGGCCATATCCGCAACGGGGTGGT
GAGCCGTATTTATACCTCCGGCGTGCGCGGCCCGCTGGCGGACGCCATCTCACGCGGCCTGTTGGCCGAACCGGTACAAA
TCCACTCCCACGGCGGTCGGGTCAACCTGATCGAATCCGGCGAATTGAGCATCGACGTTGCCTTCCTCGGGGTACCGGCC
TGCGATGAGTTCGGTAATGCCAATGGCTACAGCGGCGAAGCCTGCTGCGGCTCGCTGGGTTACGCCAAAGTGGATGCCGA
CGCGGCGGGCACCGTGGTGCTGCTGACCGAGGCGATTGTGCCTTATCCGCATCATCCGGCCAGCCTGGCGCAGGATCGGG
TGGATCTGATCGTTCAACTGGCAAGGGTGGGCGACGCCGACAAGATTGGTGCCGACGCCACGCGCATGACCTCCAACCCG
CGTGAACTGCTGATTGCCCGTCGGGCCGCCGAAGTGATCGCCGGTTCCGGCTACTTTACCGAAGGTTTCTCGCTGCAGAC
CGGCACCGGCGGCGCCTCACTGGCGGTGACGCGTTTTCTGGAAGACAAAATGCGCGCGCGCGGTATCCGCGCCGGTTTCG
CACTCGGTGGCATTACTTCGACCATGGTGGATTTGCATGAAAAAGGGCTGATCACCCGGCTGCTGGACGTGCAGAGCTTC
GACCGTGCGGCGGCGATGTCGCTGGCGCGTAACCCCAATCACATAGAAATCAGTGCCAATCAATACGCCAACTTCAGCTC
GAAGGGGGCTTCAGTCGACAGGCTTGACGTGGTGGTGCTGAGCGCGTTGGAAATCGACACCGGTTTTAACGTTAACGTGC
TGACCGGTTCCGATGGTCTGCTGCGCGGAGCTTCCGGTGGCCACTGTGACACCGCAGCCGCGGCGCGGCTGGCGATCATC
GTGGCACCGCTGGTGCGCGGGCGTATCCCAACGCTGGTCGAGCAGGTCACCACCTGTGTCACGCCCGGCTCCAGCATCGA
CATTCTGGTTACCGATCATGGTATTGCCGTCAATCCCGCTCGGCCGGAATTGGCGCAGCGGCTGCAAGAAGCCGGTTTGC
CGGTGGTCAGCATCGACTGGCTGCGCCAGCGCGCGCTGCTGCTGACCGGTGAACCTGAGCCGATCGTCTTTACCGACAAG
GTGGTGGCGGTAGTGCGCTATCGCGACGGTTCGGTGATCGACGTGGTGCATCAGGTGGCGGAGTAA

Upstream 100 bases:

>100_bases
TGGTCGACAGCCCGGTGATTGAACGCGCACGGCTGGTGCTGCAGCGTGCAGCGCTTTCCGGCATCCGCGAAGCCTCGGTT
CAGCATGGAGAGGAAGCATA

Downstream 100 bases:

>100_bases
TCCTATGGCTAACGTCCCTCCCGAACTGGCCGCCAACCGCGCAGTCAGCCTGCCGGAATTGCTCACCAGCCGTGAATGCC
GTCAGGCCCGCCAGCAGGCA

Product: citrate lyase subunit alpha

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MKRQQRLMTLNNLAELRCYQASSKANQQASKPRDIKRCDTLEQAVRRSGLQDGMTISFHHAFRGGDLALNQVMETLAAMG
FRNLTLASSSLSECHAPLVGHIRNGVVSRIYTSGVRGPLADAISRGLLAEPVQIHSHGGRVNLIESGELSIDVAFLGVPA
CDEFGNANGYSGEACCGSLGYAKVDADAAGTVVLLTEAIVPYPHHPASLAQDRVDLIVQLARVGDADKIGADATRMTSNP
RELLIARRAAEVIAGSGYFTEGFSLQTGTGGASLAVTRFLEDKMRARGIRAGFALGGITSTMVDLHEKGLITRLLDVQSF
DRAAAMSLARNPNHIEISANQYANFSSKGASVDRLDVVVLSALEIDTGFNVNVLTGSDGLLRGASGGHCDTAAAARLAII
VAPLVRGRIPTLVEQVTTCVTPGSSIDILVTDHGIAVNPARPELAQRLQEAGLPVVSIDWLRQRALLLTGEPEPIVFTDK
VVAVVRYRDGSVIDVVHQVAE

Sequences:

>Translated_501_residues
MKRQQRLMTLNNLAELRCYQASSKANQQASKPRDIKRCDTLEQAVRRSGLQDGMTISFHHAFRGGDLALNQVMETLAAMG
FRNLTLASSSLSECHAPLVGHIRNGVVSRIYTSGVRGPLADAISRGLLAEPVQIHSHGGRVNLIESGELSIDVAFLGVPA
CDEFGNANGYSGEACCGSLGYAKVDADAAGTVVLLTEAIVPYPHHPASLAQDRVDLIVQLARVGDADKIGADATRMTSNP
RELLIARRAAEVIAGSGYFTEGFSLQTGTGGASLAVTRFLEDKMRARGIRAGFALGGITSTMVDLHEKGLITRLLDVQSF
DRAAAMSLARNPNHIEISANQYANFSSKGASVDRLDVVVLSALEIDTGFNVNVLTGSDGLLRGASGGHCDTAAAARLAII
VAPLVRGRIPTLVEQVTTCVTPGSSIDILVTDHGIAVNPARPELAQRLQEAGLPVVSIDWLRQRALLLTGEPEPIVFTDK
VVAVVRYRDGSVIDVVHQVAE
>Mature_501_residues
MKRQQRLMTLNNLAELRCYQASSKANQQASKPRDIKRCDTLEQAVRRSGLQDGMTISFHHAFRGGDLALNQVMETLAAMG
FRNLTLASSSLSECHAPLVGHIRNGVVSRIYTSGVRGPLADAISRGLLAEPVQIHSHGGRVNLIESGELSIDVAFLGVPA
CDEFGNANGYSGEACCGSLGYAKVDADAAGTVVLLTEAIVPYPHHPASLAQDRVDLIVQLARVGDADKIGADATRMTSNP
RELLIARRAAEVIAGSGYFTEGFSLQTGTGGASLAVTRFLEDKMRARGIRAGFALGGITSTMVDLHEKGLITRLLDVQSF
DRAAAMSLARNPNHIEISANQYANFSSKGASVDRLDVVVLSALEIDTGFNVNVLTGSDGLLRGASGGHCDTAAAARLAII
VAPLVRGRIPTLVEQVTTCVTPGSSIDILVTDHGIAVNPARPELAQRLQEAGLPVVSIDWLRQRALLLTGEPEPIVFTDK
VVAVVRYRDGSVIDVVHQVAE

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=502, Percent_Identity=79.0836653386454, Blast_Score=816, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 53239; Mature: 53239

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQQRLMTLNNLAELRCYQASSKANQQASKPRDIKRCDTLEQAVRRSGLQDGMTISFHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEE
AFRGGDLALNQVMETLAAMGFRNLTLASSSLSECHAPLVGHIRNGVVSRIYTSGVRGPLA
CCCCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
DAISRGLLAEPVQIHSHGGRVNLIESGELSIDVAFLGVPACDEFGNANGYSGEACCGSLG
HHHHCCCCCCCCEEECCCCEEEEEECCCEEEEEEEEECCCHHHCCCCCCCCCCHHHCCCC
YAKVDADAAGTVVLLTEAIVPYPHHPASLAQDRVDLIVQLARVGDADKIGADATRMTSNP
CEEECCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCHHHCCCCH
RELLIARRAAEVIAGSGYFTEGFSLQTGTGGASLAVTRFLEDKMRARGIRAGFALGGITS
HHHHHHHHHHHHHCCCCCEECCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHH
TMVDLHEKGLITRLLDVQSFDRAAAMSLARNPNHIEISANQYANFSSKGASVDRLDVVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHEEEE
SALEIDTGFNVNVLTGSDGLLRGASGGHCDTAAAARLAIIVAPLVRGRIPTLVEQVTTCV
EHEEECCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
TPGSSIDILVTDHGIAVNPARPELAQRLQEAGLPVVSIDWLRQRALLLTGEPEPIVFTDK
CCCCCEEEEEECCCEEECCCCHHHHHHHHHCCCCEEEHHHHHCCEEEEECCCCCEEEECC
VVAVVRYRDGSVIDVVHQVAE
EEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MKRQQRLMTLNNLAELRCYQASSKANQQASKPRDIKRCDTLEQAVRRSGLQDGMTISFHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEE
AFRGGDLALNQVMETLAAMGFRNLTLASSSLSECHAPLVGHIRNGVVSRIYTSGVRGPLA
CCCCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
DAISRGLLAEPVQIHSHGGRVNLIESGELSIDVAFLGVPACDEFGNANGYSGEACCGSLG
HHHHCCCCCCCCEEECCCCEEEEEECCCEEEEEEEEECCCHHHCCCCCCCCCCHHHCCCC
YAKVDADAAGTVVLLTEAIVPYPHHPASLAQDRVDLIVQLARVGDADKIGADATRMTSNP
CEEECCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCHHHCCCCH
RELLIARRAAEVIAGSGYFTEGFSLQTGTGGASLAVTRFLEDKMRARGIRAGFALGGITS
HHHHHHHHHHHHHCCCCCEECCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHH
TMVDLHEKGLITRLLDVQSFDRAAAMSLARNPNHIEISANQYANFSSKGASVDRLDVVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHEEEE
SALEIDTGFNVNVLTGSDGLLRGASGGHCDTAAAARLAIIVAPLVRGRIPTLVEQVTTCV
EHEEECCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
TPGSSIDILVTDHGIAVNPARPELAQRLQEAGLPVVSIDWLRQRALLLTGEPEPIVFTDK
CCCCCEEEEEECCCEEECCCCHHHHHHHHHCCCCEEEHHHHHCCEEEEECCCCCEEEECC
VVAVVRYRDGSVIDVVHQVAE
EEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]