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The map label for this gene is gcvA [H]

Identifier: 157371386

GI number: 157371386

Start: 3464967

End: 3465848

Strand: Reverse

Name: gcvA [H]

Synonym: Spro_3147

Alternate gene names: 157371386

Gene position: 3465848-3464967 (Counterclockwise)

Preceding gene: 157371389

Following gene: 157371385

Centisome position: 63.61

GC content: 60.43

Gene sequence:

>882_bases
ATGTTTGCCACGCTTCCGGTTACTGCATTGCGCACCTTTGAATCCGCCGCCCGCCTGCGCAGTTTTAAACAGGCTGCACT
GGAGCTGGCTGTAACCCCGACGGCGGTATCGCATCAGATCAAGGCGCTTGAGCAGCAACTGGGTTTTGCCTTGTTCGATC
GGGTGCCGCGCGGTGTGCGGTTAACGCAGAAGGGCGAGCGTTTGTTCGCTGGGGTGCATACCGCTTTGCTCGATGTGGCA
ACCACGCTGGATGCGCTACGACCGGTTCCCAGTACCGGCTCACTGTGTGTTTCCGTCACCCATTCTTTCGCTGCATTGTG
GCTGGTCCCGCGCCTGGGGCGCTTTTATCAAGCTTACCCGCACTACCTGGTACGCCTGGAGGCCTGCGGTGAGGTGATTG
ATCTGCAGCAGGACGCCAGCGTGGACATTGCCATTCGCTACAGCAGTACTGACTATCCGCAGTTGCATCGGGCAGCGCGG
TTGGAAGAGAGCTTTGGCGTTTACGGCGCCCCGGATTTGTGTGCAACTGAGCAACAAGCGCCCACGCTGATCACCGTTCA
CTGGCATGACTCCACTCTTTATGAAACCGGCTGGCGCGAGTGGTGCCAGGCTGCGGGTGTCGACTGGTGGCAACGGCATA
CCGCGTTGCGGGCTTATGACGAAGAGCATTATGCTTTGCAGGCAGCGGTGGCCGGGCAGGGGCTGGTACTGGCCAGTTCG
GTGATGGTGTCGGACCTGGTGCGTAACGGGTTGTTGGCATCCTATCGGCCGGAAATTTGCGTACCGGGTGCGGCTTATAG
CGCGCTGTGCGTGCCTGGGCGCGAACGGCATCCGCCGGTACGGGCGTTTCTGGCCTGGCTGCAACTGGAAATCTTGCGCT
GA

Upstream 100 bases:

>100_bases
CATGGATATACGATTGCCAATTTACGGTGCACAAAGAGTCAGTGACAAATGACGGCTATTCTTTCAGGATGAGTTAAATT
CATCTGTATGGGATTTGATC

Downstream 100 bases:

>100_bases
ACCACAGCACAAAATAGAAATTCTTCTGGCGTGAGGCATGGGAATATCAGATAACTTTGTTATTCCCTTCCCATGAGAGA
GATGCCCATGAGCCAGCCAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MFATLPVTALRTFESAARLRSFKQAALELAVTPTAVSHQIKALEQQLGFALFDRVPRGVRLTQKGERLFAGVHTALLDVA
TTLDALRPVPSTGSLCVSVTHSFAALWLVPRLGRFYQAYPHYLVRLEACGEVIDLQQDASVDIAIRYSSTDYPQLHRAAR
LEESFGVYGAPDLCATEQQAPTLITVHWHDSTLYETGWREWCQAAGVDWWQRHTALRAYDEEHYALQAAVAGQGLVLASS
VMVSDLVRNGLLASYRPEICVPGAAYSALCVPGRERHPPVRAFLAWLQLEILR

Sequences:

>Translated_293_residues
MFATLPVTALRTFESAARLRSFKQAALELAVTPTAVSHQIKALEQQLGFALFDRVPRGVRLTQKGERLFAGVHTALLDVA
TTLDALRPVPSTGSLCVSVTHSFAALWLVPRLGRFYQAYPHYLVRLEACGEVIDLQQDASVDIAIRYSSTDYPQLHRAAR
LEESFGVYGAPDLCATEQQAPTLITVHWHDSTLYETGWREWCQAAGVDWWQRHTALRAYDEEHYALQAAVAGQGLVLASS
VMVSDLVRNGLLASYRPEICVPGAAYSALCVPGRERHPPVRAFLAWLQLEILR
>Mature_293_residues
MFATLPVTALRTFESAARLRSFKQAALELAVTPTAVSHQIKALEQQLGFALFDRVPRGVRLTQKGERLFAGVHTALLDVA
TTLDALRPVPSTGSLCVSVTHSFAALWLVPRLGRFYQAYPHYLVRLEACGEVIDLQQDASVDIAIRYSSTDYPQLHRAAR
LEESFGVYGAPDLCATEQQAPTLITVHWHDSTLYETGWREWCQAAGVDWWQRHTALRAYDEEHYALQAAVAGQGLVLASS
VMVSDLVRNGLLASYRPEICVPGAAYSALCVPGRERHPPVRAFLAWLQLEILR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=33.1081081081081, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=28.3783783783784, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786448, Length=254, Percent_Identity=31.1023622047244, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787128, Length=146, Percent_Identity=31.5068493150685, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI157672245, Length=211, Percent_Identity=31.2796208530806, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=25.085910652921, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=289, Percent_Identity=26.2975778546713, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87081978, Length=279, Percent_Identity=28.3154121863799, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789440, Length=247, Percent_Identity=25.1012145748988, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32513; Mature: 32513

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFATLPVTALRTFESAARLRSFKQAALELAVTPTAVSHQIKALEQQLGFALFDRVPRGVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LTQKGERLFAGVHTALLDVATTLDALRPVPSTGSLCVSVTHSFAALWLVPRLGRFYQAYP
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HYLVRLEACGEVIDLQQDASVDIAIRYSSTDYPQLHRAARLEESFGVYGAPDLCATEQQA
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PTLITVHWHDSTLYETGWREWCQAAGVDWWQRHTALRAYDEEHYALQAAVAGQGLVLASS
CEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHH
VMVSDLVRNGLLASYRPEICVPGAAYSALCVPGRERHPPVRAFLAWLQLEILR
HHHHHHHHCCCHHCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFATLPVTALRTFESAARLRSFKQAALELAVTPTAVSHQIKALEQQLGFALFDRVPRGVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LTQKGERLFAGVHTALLDVATTLDALRPVPSTGSLCVSVTHSFAALWLVPRLGRFYQAYP
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HYLVRLEACGEVIDLQQDASVDIAIRYSSTDYPQLHRAARLEESFGVYGAPDLCATEQQA
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PTLITVHWHDSTLYETGWREWCQAAGVDWWQRHTALRAYDEEHYALQAAVAGQGLVLASS
CEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHH
VMVSDLVRNGLLASYRPEICVPGAAYSALCVPGRERHPPVRAFLAWLQLEILR
HHHHHHHHCCCHHCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]