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The map label for this gene is yafC [C]

Identifier: 157371330

GI number: 157371330

Start: 3400918

End: 3401823

Strand: Reverse

Name: yafC [C]

Synonym: Spro_3091

Alternate gene names: 157371330

Gene position: 3401823-3400918 (Counterclockwise)

Preceding gene: 157371333

Following gene: 157371329

Centisome position: 62.43

GC content: 63.25

Gene sequence:

>906_bases
ATGGATCGGTTGGACGAACTGGCGATTTTTGTAGCGGTGATCCAGCACGGCAGCCTGGCCGCCGCCGGTCGCAAACTGCG
ACGTTCCGCCCCGGCAGTGACCCGGGCCATCGCCTCGCTGGAGCAGCGTTTTGGCGCCCGGCTGGTTGAACGCACCACCC
GGCGGCTGGCCCCGACCGAAGCCGGGCTGCGGCTGGCGGAGCGCGCGCATTTACTGCTGCATGAATACCAGGCGGCGGTA
CTGGATACGGCGGATACTCAGCTCAGCGGCCTGCTGCGCATTACCTCGCCGGTGCAGTTCGGGCGCAAGCACGTGGCACC
GGTAGTCATGGCGTTTCTCGATCTTTATCCGCAGATGCAAATTGAAATGGTGCTCAACGATCGCAACCTGGATTTGATCG
ACGAGGGGCTGGACGTGGCGGTGCGCATCGGCCATTTGCAGGACTCTTCCCGCGTGGCGCGTCGGTTGGGGCAGGTATCC
CGGGTGACGGTCGCCAGTCCGGCCTATCTGGCGCTACGCGGCGAGCCATTGGAACCGGCGCAGTTGGTCGATCATGATAC
CATCGTCGGCACCCAGCGGGCGTCGCTGCGCGAATGGCGGTTTGGCCCACAGGAAAACGGTGACAGGGTAAGGCTGACGC
CGCGCTTGCTGCTCAACGAAGTCGAAACCCAGTTGCTGGCGGCGCGAGCCGGCAAGGGCATCGCCCGGTTGCTGTCGTAT
CAGGTGGCAGACGATCTGGCAGCGGGGACGCTGGTGCGTTTGCTGCCGGCGTTTGAACCCTTGCCGATGCCGGTGCAGCT
GGTGGCGCAAAATAGCCAACGCATGCCGCTAAAGGTGCGGACCTTCTGGGATTATGCCTGGCAGGAATTAAGTCAATTGG
CGCAAATTCAGCCGCCGTTAAAATAG

Upstream 100 bases:

>100_bases
ATAATCACACTCCTGAAGGATTTTAATCAGCATACTCTTGCAGTCGGTGAGCGAAATCCGGCAATCTGGAAATTAATAAT
TTCAATTTATGGAATAGTCT

Downstream 100 bases:

>100_bases
CGATTCAGGCTCAATAAGTTTCACTGGCTAAACTTATTGAGCCGCGGAAAAGATTTATATTACCCTGCACGCATTGCTGC
TGTTCCCTTCTTGACAATAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDRLDELAIFVAVIQHGSLAAAGRKLRRSAPAVTRAIASLEQRFGARLVERTTRRLAPTEAGLRLAERAHLLLHEYQAAV
LDTADTQLSGLLRITSPVQFGRKHVAPVVMAFLDLYPQMQIEMVLNDRNLDLIDEGLDVAVRIGHLQDSSRVARRLGQVS
RVTVASPAYLALRGEPLEPAQLVDHDTIVGTQRASLREWRFGPQENGDRVRLTPRLLLNEVETQLLAARAGKGIARLLSY
QVADDLAAGTLVRLLPAFEPLPMPVQLVAQNSQRMPLKVRTFWDYAWQELSQLAQIQPPLK

Sequences:

>Translated_301_residues
MDRLDELAIFVAVIQHGSLAAAGRKLRRSAPAVTRAIASLEQRFGARLVERTTRRLAPTEAGLRLAERAHLLLHEYQAAV
LDTADTQLSGLLRITSPVQFGRKHVAPVVMAFLDLYPQMQIEMVLNDRNLDLIDEGLDVAVRIGHLQDSSRVARRLGQVS
RVTVASPAYLALRGEPLEPAQLVDHDTIVGTQRASLREWRFGPQENGDRVRLTPRLLLNEVETQLLAARAGKGIARLLSY
QVADDLAAGTLVRLLPAFEPLPMPVQLVAQNSQRMPLKVRTFWDYAWQELSQLAQIQPPLK
>Mature_301_residues
MDRLDELAIFVAVIQHGSLAAAGRKLRRSAPAVTRAIASLEQRFGARLVERTTRRLAPTEAGLRLAERAHLLLHEYQAAV
LDTADTQLSGLLRITSPVQFGRKHVAPVVMAFLDLYPQMQIEMVLNDRNLDLIDEGLDVAVRIGHLQDSSRVARRLGQVS
RVTVASPAYLALRGEPLEPAQLVDHDTIVGTQRASLREWRFGPQENGDRVRLTPRLLLNEVETQLLAARAGKGIARLLSY
QVADDLAAGTLVRLLPAFEPLPMPVQLVAQNSQRMPLKVRTFWDYAWQELSQLAQIQPPLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=33.4507042253521, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1789639, Length=303, Percent_Identity=31.6831683168317, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1789440, Length=256, Percent_Identity=31.25, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787128, Length=299, Percent_Identity=28.7625418060201, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI145693193, Length=289, Percent_Identity=29.0657439446367, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI87081978, Length=258, Percent_Identity=32.1705426356589, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787589, Length=260, Percent_Identity=31.1538461538462, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=27.3037542662116, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788706, Length=254, Percent_Identity=23.2283464566929, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33510; Mature: 33510

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLDELAIFVAVIQHGSLAAAGRKLRRSAPAVTRAIASLEQRFGARLVERTTRRLAPTE
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGLRLAERAHLLLHEYQAAVLDTADTQLSGLLRITSPVQFGRKHVAPVVMAFLDLYPQMQ
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEE
IEMVLNDRNLDLIDEGLDVAVRIGHLQDSSRVARRLGQVSRVTVASPAYLALRGEPLEPA
EEEEECCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHEEEECCCEEEEEECCCCCHH
QLVDHDTIVGTQRASLREWRFGPQENGDRVRLTPRLLLNEVETQLLAARAGKGIARLLSY
HHHCCCHHCCCCHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCHHHHHHHHH
QVADDLAAGTLVRLLPAFEPLPMPVQLVAQNSQRMPLKVRTFWDYAWQELSQLAQIQPPL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCC
K
C
>Mature Secondary Structure
MDRLDELAIFVAVIQHGSLAAAGRKLRRSAPAVTRAIASLEQRFGARLVERTTRRLAPTE
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGLRLAERAHLLLHEYQAAVLDTADTQLSGLLRITSPVQFGRKHVAPVVMAFLDLYPQMQ
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEE
IEMVLNDRNLDLIDEGLDVAVRIGHLQDSSRVARRLGQVSRVTVASPAYLALRGEPLEPA
EEEEECCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHEEEECCCEEEEEECCCCCHH
QLVDHDTIVGTQRASLREWRFGPQENGDRVRLTPRLLLNEVETQLLAARAGKGIARLLSY
HHHCCCHHCCCCHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCHHHHHHHHH
QVADDLAAGTLVRLLPAFEPLPMPVQLVAQNSQRMPLKVRTFWDYAWQELSQLAQIQPPL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]