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The map label for this gene is mhpE

Identifier: 157371265

GI number: 157371265

Start: 3328606

End: 3329607

Strand: Reverse

Name: mhpE

Synonym: Spro_3025

Alternate gene names: 157371265

Gene position: 3329607-3328606 (Counterclockwise)

Preceding gene: 157371266

Following gene: 157371264

Centisome position: 61.11

GC content: 59.58

Gene sequence:

>1002_bases
ATGACTATTCTCATTAACGATTCAACCCTGCGCGATGGTCAGCATGCGGTAAAACACCAACTCACTGCGGCGCAGTTGCG
AAGCTACGCCACGGCGGCAGACAAAACCGGCGTGGCGATAGTTGAAGTCGGCCACGGTAACGGGCTGGGGGCTTCCTCCT
ATCAGGTGGGGCGTGCGGCATTGAGTGACGAAGAGATGCTGACCACCGTGCGCGAGTCTTTGCAAAACAGCAAGATGGGG
GTGTTTATGTTGCCCGGTTGGGGCACCATCGCCGACTTGACCAAAGCTCTGGCTTACGGCACGGATGTGGTGCGCATTGG
TACCCATTGTACCGAGGCGACGCTGGCCGAACGCCATCTGGGTTGGCTGCGGGAACAGGGCGCTGAGGCTCATGCGGTGC
TGATGATGAGCCATATGGCAAGCCCGGCCGAGTTGGCTGACCAGGCTGAGTTATTGGTGGGTTACGGCGCCCAGGCGGTC
GGCATTATGGATTCGGCCGGCAGTCTTTTGCCGCAGGATGTCACCGCACGTATTACGGCCATGCGCATGCAGGTAAAGGT
GCCGCTGATTTTCCACGCGCATAACAACCTGGGCATGGCGGTGGCGAATTCGCTGGCCGCCGTTCAGGCGGGCGCCGGCA
TTATTGACGGCTGCGCTCGTGGGTTTGGTGCCGGGGCGGGCAATACCCAACTGGAAGTGCTGATCCCGGTATTGGAGCGC
CTGGGTTTCAACACCGGCATCGATCTCTATCATTTTCTGGATGCCGCCGATCTCGCCGCCAGAGAGTTAATGGTGGTTCC
GCCAATGATTGACTCTCTGGGCATCGTCAGCGGTCTGGCCGGTGTCTTCTCCGGCTTCAAAAGCCCGGTGCTAAACCATG
CAGGCAGCGCCGGGGTGGATGCGCGCGATGTCTTCTTCGAACTGGGACGACGACAAATTATTGCCGGGCAGGAAGACCTG
ATCGTTGAGGTTGTCGCCGAACTGAAGAGGGCGGCCTCATGA

Upstream 100 bases:

>100_bases
GTGATGCCTGAATACGCGGGCAACCTGGACCTGATTAATTCCGCTGCCATTCTGGTTGCCGAACAGTATGCGCACTATCG
AAATAAAGCGGAGGTGGCCA

Downstream 100 bases:

>100_bases
CCATGTTAATCATCGACGCCGCTGAAATGGCGGGGGGTGATCGTCTGCGACAGGTGATGATCGCCCTGAGTGACGTGTTC
CAGGATTTACGCAGCGGTAA

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTILINDSTLRDGQHAVKHQLTAAQLRSYATAADKTGVAIVEVGHGNGLGASSYQVGRAALSDEEMLTTVRESLQNSKMG
VFMLPGWGTIADLTKALAYGTDVVRIGTHCTEATLAERHLGWLREQGAEAHAVLMMSHMASPAELADQAELLVGYGAQAV
GIMDSAGSLLPQDVTARITAMRMQVKVPLIFHAHNNLGMAVANSLAAVQAGAGIIDGCARGFGAGAGNTQLEVLIPVLER
LGFNTGIDLYHFLDAADLAARELMVVPPMIDSLGIVSGLAGVFSGFKSPVLNHAGSAGVDARDVFFELGRRQIIAGQEDL
IVEVVAELKRAAS

Sequences:

>Translated_333_residues
MTILINDSTLRDGQHAVKHQLTAAQLRSYATAADKTGVAIVEVGHGNGLGASSYQVGRAALSDEEMLTTVRESLQNSKMG
VFMLPGWGTIADLTKALAYGTDVVRIGTHCTEATLAERHLGWLREQGAEAHAVLMMSHMASPAELADQAELLVGYGAQAV
GIMDSAGSLLPQDVTARITAMRMQVKVPLIFHAHNNLGMAVANSLAAVQAGAGIIDGCARGFGAGAGNTQLEVLIPVLER
LGFNTGIDLYHFLDAADLAARELMVVPPMIDSLGIVSGLAGVFSGFKSPVLNHAGSAGVDARDVFFELGRRQIIAGQEDL
IVEVVAELKRAAS
>Mature_332_residues
TILINDSTLRDGQHAVKHQLTAAQLRSYATAADKTGVAIVEVGHGNGLGASSYQVGRAALSDEEMLTTVRESLQNSKMGV
FMLPGWGTIADLTKALAYGTDVVRIGTHCTEATLAERHLGWLREQGAEAHAVLMMSHMASPAELADQAELLVGYGAQAVG
IMDSAGSLLPQDVTARITAMRMQVKVPLIFHAHNNLGMAVANSLAAVQAGAGIIDGCARGFGAGAGNTQLEVLIPVLERL
GFNTGIDLYHFLDAADLAARELMVVPPMIDSLGIVSGLAGVFSGFKSPVLNHAGSAGVDARDVFFELGRRQIIAGQEDLI
VEVVAELKRAAS

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=329, Percent_Identity=41.9452887537994, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786261, Length=252, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA_SERP5 (A8GG86)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479254.1
- ProteinModelPortal:   A8GG86
- SMR:   A8GG86
- STRING:   A8GG86
- GeneID:   5604073
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3025
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   MMAHMAS
- ProtClustDB:   PRK08195
- BioCyc:   SPRO399741:SPRO_3025-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 34906; Mature: 34774

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 165-165 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILINDSTLRDGQHAVKHQLTAAQLRSYATAADKTGVAIVEVGHGNGLGASSYQVGRAA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHH
LSDEEMLTTVRESLQNSKMGVFMLPGWGTIADLTKALAYGTDVVRIGTHCTEATLAERHL
CCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
GWLREQGAEAHAVLMMSHMASPAELADQAELLVGYGAQAVGIMDSAGSLLPQDVTARITA
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHH
MRMQVKVPLIFHAHNNLGMAVANSLAAVQAGAGIIDGCARGFGAGAGNTQLEVLIPVLER
HHEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
LGFNTGIDLYHFLDAADLAARELMVVPPMIDSLGIVSGLAGVFSGFKSPVLNHAGSAGVD
CCCCCCCHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ARDVFFELGRRQIIAGQEDLIVEVVAELKRAAS
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TILINDSTLRDGQHAVKHQLTAAQLRSYATAADKTGVAIVEVGHGNGLGASSYQVGRAA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHH
LSDEEMLTTVRESLQNSKMGVFMLPGWGTIADLTKALAYGTDVVRIGTHCTEATLAERHL
CCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
GWLREQGAEAHAVLMMSHMASPAELADQAELLVGYGAQAVGIMDSAGSLLPQDVTARITA
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHH
MRMQVKVPLIFHAHNNLGMAVANSLAAVQAGAGIIDGCARGFGAGAGNTQLEVLIPVLER
HHEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
LGFNTGIDLYHFLDAADLAARELMVVPPMIDSLGIVSGLAGVFSGFKSPVLNHAGSAGVD
CCCCCCCHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ARDVFFELGRRQIIAGQEDLIVEVVAELKRAAS
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA