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The map label for this gene is putP [H]

Identifier: 157371170

GI number: 157371170

Start: 3228300

End: 3229784

Strand: Reverse

Name: putP [H]

Synonym: Spro_2930

Alternate gene names: 157371170

Gene position: 3229784-3228300 (Counterclockwise)

Preceding gene: 157371174

Following gene: 157371169

Centisome position: 59.27

GC content: 56.43

Gene sequence:

>1485_bases
ATGACAATGAGCACACCGATGCTGGTGACCTTCCTGGTGTATATCTTCGGGATGGTGTTAATCGGCTTACTTGCCTATCG
GGCAACCAATAACTTTGACGACTACATTCTGGGCGGTCGTAGCCTGGGTAGCGTAGTGACCGCGCTTTCTGCCGGCGCAT
CAGACATGAGCGGCTGGCTGTTGATGGGCCTGCCGGGCGCCATCTTCCTGTCCGGCATCTCCGAAAGCTGGATTGCCATT
GGCCTGACGATCGGTGCTTATTTCAACTGGAAGCTGGTGGCAGGGCGGCTGCGCGTGCATACCGAAGCCAACAATAATGC
CCTGACGCTGCCGGATTACTTCACCAGCCGTTTTGAAGACAACAGTAAGTTGCTGCGGGTGATTTCCGCCATTGTTATCC
TGGTGTTCTTCACTATCTACTGTGCTTCCGGCATTGTGGCCGGTGCGCGCCTGTTCGAGAGCACCTTCGGGATGAGCTAC
GAAACCGCGCTGTGGGCCGGTGCTGCTGCCACTATCCTTTATACCTTTATCGGCGGATTCCTGGCGGTGAGCTGGACCGA
TACCGTGCAGGCCAGCCTGATGATTTTCGCGCTGATCCTGACGCCGGTGATTGTTATCTTCGCGGTTGGCGGCATTGATA
CCTCGATGATGGTGATTGAGGCCAAGAATCCGGCCAATCTCGATATGCTGAAAGGTTTGAACTTTGTGGCGATCCTCTCG
CTGCTGGGTTGGGGCCTGGGTTACTTCGGTCAGCCACACATTCTGGCGCGCTTTATGGCGGCAGACTCTCACCGCACCAT
TCGCAGCGCACGCCGCATCAGCATGACCTGGATGATCCTGTGTCTGGCCGGTACCATTGCGGTCGGTTTCTTCGGGATTG
CCTACTTCGCCAATAACCCGGATCAGGCGGGCAATGTATCGCAGAACGGTGAGCGCGTGTTTATCGAATTGGCGATGCTT
CTGTTCAACCCGTGGGTAGCGGGCATTCTGCTGTCGGCGATTTTGGCGGCGGTAATGAGTACTCTGAGCTGCCAACTGTT
GGTGTGTTCAAGTGCAATTACCGAAGACCTGTACAAGGCCTTCCTGCGTAAGGGTGCCAGCCAGCGTGAGCTGGTGTGGG
TCGGGCGCTTCATGGTGTTGCTGGTGGCGTTGATTGCCATCGCGCTGGCGGCAAACCCGGAAAACCGCGTACTGGGTCTG
GTGAGCTACGCCTGGGCCGGCTTCGGTGCAGCCTTTGGTCCAGTGGTGTTGATCTCGGTAATGTGGTCACGCATGACGCG
TAATGGCGCTTTGGCCGGTATGTTGGTGGGCGCGGCAACGGTGATCGTCTGGAAACAGTTTGCCTGGTTGGATTTGTATG
AAATTATCCCGGGCTTCCTGTTCGCTTGTATCGCCATCGTGGTGGTGAGCCTGATGGGGCGTCAGCCTTCGGCAACCATC
ACCGAACGTTTTGATCAGGCAGAAGCCGAGTTCAAAACCGTTTAA

Upstream 100 bases:

>100_bases
TTCACCTGCGGGGGGCCCGCAATTTCGCCACGTTCCGGCGGATTGGCAAGCAGTAACTATCGCAATACAGCGCGAAATAA
TAATGAGAGTGGAGAATTTC

Downstream 100 bases:

>100_bases
TCCTCTCGAGTTAATCATCTCAAAGGGCGCTTAATGCGCCCTTAGGTTGCTGACAAAGCAGGAAAAAACGTGGTTTTTCC
TGCTTTGTGGTGATCAGCCG

Product: sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTMSTPMLVTFLVYIFGMVLIGLLAYRATNNFDDYILGGRSLGSVVTALSAGASDMSGWLLMGLPGAIFLSGISESWIAI
GLTIGAYFNWKLVAGRLRVHTEANNNALTLPDYFTSRFEDNSKLLRVISAIVILVFFTIYCASGIVAGARLFESTFGMSY
ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIFAVGGIDTSMMVIEAKNPANLDMLKGLNFVAILS
LLGWGLGYFGQPHILARFMAADSHRTIRSARRISMTWMILCLAGTIAVGFFGIAYFANNPDQAGNVSQNGERVFIELAML
LFNPWVAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKGASQRELVWVGRFMVLLVALIAIALAANPENRVLGL
VSYAWAGFGAAFGPVVLISVMWSRMTRNGALAGMLVGAATVIVWKQFAWLDLYEIIPGFLFACIAIVVVSLMGRQPSATI
TERFDQAEAEFKTV

Sequences:

>Translated_494_residues
MTMSTPMLVTFLVYIFGMVLIGLLAYRATNNFDDYILGGRSLGSVVTALSAGASDMSGWLLMGLPGAIFLSGISESWIAI
GLTIGAYFNWKLVAGRLRVHTEANNNALTLPDYFTSRFEDNSKLLRVISAIVILVFFTIYCASGIVAGARLFESTFGMSY
ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIFAVGGIDTSMMVIEAKNPANLDMLKGLNFVAILS
LLGWGLGYFGQPHILARFMAADSHRTIRSARRISMTWMILCLAGTIAVGFFGIAYFANNPDQAGNVSQNGERVFIELAML
LFNPWVAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKGASQRELVWVGRFMVLLVALIAIALAANPENRVLGL
VSYAWAGFGAAFGPVVLISVMWSRMTRNGALAGMLVGAATVIVWKQFAWLDLYEIIPGFLFACIAIVVVSLMGRQPSATI
TERFDQAEAEFKTV
>Mature_493_residues
TMSTPMLVTFLVYIFGMVLIGLLAYRATNNFDDYILGGRSLGSVVTALSAGASDMSGWLLMGLPGAIFLSGISESWIAIG
LTIGAYFNWKLVAGRLRVHTEANNNALTLPDYFTSRFEDNSKLLRVISAIVILVFFTIYCASGIVAGARLFESTFGMSYE
TALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIFAVGGIDTSMMVIEAKNPANLDMLKGLNFVAILSL
LGWGLGYFGQPHILARFMAADSHRTIRSARRISMTWMILCLAGTIAVGFFGIAYFANNPDQAGNVSQNGERVFIELAMLL
FNPWVAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKGASQRELVWVGRFMVLLVALIAIALAANPENRVLGLV
SYAWAGFGAAFGPVVLISVMWSRMTRNGALAGMLVGAATVIVWKQFAWLDLYEIIPGFLFACIAIVVVSLMGRQPSATIT
ERFDQAEAEFKTV

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=492, Percent_Identity=45.9349593495935, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI167466278, Length=428, Percent_Identity=24.2990654205607, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI110835708, Length=503, Percent_Identity=23.4592445328032, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1787251, Length=493, Percent_Identity=82.7586206896552, Blast_Score=805, Evalue=0.0,
Organism=Escherichia coli, GI1790503, Length=446, Percent_Identity=26.9058295964126, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082237, Length=485, Percent_Identity=26.8041237113402, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115533094, Length=430, Percent_Identity=24.8837209302326, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221459584, Length=382, Percent_Identity=21.9895287958115, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24645928, Length=356, Percent_Identity=24.1573033707865, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24651741, Length=465, Percent_Identity=22.3655913978495, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221459588, Length=374, Percent_Identity=23.7967914438503, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53407; Mature: 53276

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSTPMLVTFLVYIFGMVLIGLLAYRATNNFDDYILGGRSLGSVVTALSAGASDMSGWL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCEE
LMGLPGAIFLSGISESWIAIGLTIGAYFNWKLVAGRLRVHTEANNNALTLPDYFTSRFED
EECCCHHHHHHCCCCCHHEEEEEHHCEEEEEEEECEEEEEEECCCCEEECCHHHHHHCCC
NSKLLRVISAIVILVFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATILYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQASLMIFALILTPVIVIFAVGGIDTSMMVIEAKNPANLDMLKGLNFVAILS
HEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCHHHHHHH
LLGWGLGYFGQPHILARFMAADSHRTIRSARRISMTWMILCLAGTIAVGFFGIAYFANNP
HHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DQAGNVSQNGERVFIELAMLLFNPWVAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKGASQRELVWVGRFMVLLVALIAIALAANPENRVLGLVSYAWAGFGAAFGPVVLISV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHCHHHHHHHHHHH
MWSRMTRNGALAGMLVGAATVIVWKQFAWLDLYEIIPGFLFACIAIVVVSLMGRQPSATI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
TERFDQAEAEFKTV
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TMSTPMLVTFLVYIFGMVLIGLLAYRATNNFDDYILGGRSLGSVVTALSAGASDMSGWL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCEE
LMGLPGAIFLSGISESWIAIGLTIGAYFNWKLVAGRLRVHTEANNNALTLPDYFTSRFED
EECCCHHHHHHCCCCCHHEEEEEHHCEEEEEEEECEEEEEEECCCCEEECCHHHHHHCCC
NSKLLRVISAIVILVFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATILYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQASLMIFALILTPVIVIFAVGGIDTSMMVIEAKNPANLDMLKGLNFVAILS
HEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCHHHHHHH
LLGWGLGYFGQPHILARFMAADSHRTIRSARRISMTWMILCLAGTIAVGFFGIAYFANNP
HHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DQAGNVSQNGERVFIELAMLLFNPWVAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKGASQRELVWVGRFMVLLVALIAIALAANPENRVLGLVSYAWAGFGAAFGPVVLISV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHCHHHHHHHHHHH
MWSRMTRNGALAGMLVGAATVIVWKQFAWLDLYEIIPGFLFACIAIVVVSLMGRQPSATI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
TERFDQAEAEFKTV
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]