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The map label for this gene is gapA [H]

Identifier: 157370968

GI number: 157370968

Start: 3004661

End: 3005656

Strand: Direct

Name: gapA [H]

Synonym: Spro_2728

Alternate gene names: 157370968

Gene position: 3004661-3005656 (Clockwise)

Preceding gene: 157370965

Following gene: 157370969

Centisome position: 55.14

GC content: 49.9

Gene sequence:

>996_bases
ATGACTATCAAAGTAGGTATCAACGGTTTTGGCCGTATCGGTCGCATTGTTTTTCGTGCTGCTCAAGAACGTTCTGACAT
CGAAATCGTTGCAATCAACGACCTGTTAGACGCAGAGTACATGGCTTACATGCTGAAATATGACTCTACTCACGGCCGCT
TCAACGGCACTGTAGAAGTTAAAGACGGCCACCTGGTTGTTAACGGTAAAACCATCCGTTGTACCTCAGAAAGAGATCCG
GCTAACCTGAAGTGGAACGAAGTCAACGTTGACGTCGTTGCTGAAGCAACTGGCCTGTTCCTGACTGACGAAACTGCACG
TAAACACATCGCTGCAGGCGCTAAGAAAGTTGTTCTGACTGGCCCATCCAAAGACGACACCCCAATGTTCGTTATGGGTG
TTAACCACAAATCCTACGCTGGCCAGGAAATCGTTTCTAACGCTTCTTGCACCACCAACTGCCTGGCACCACTGGCTAAA
GTTATCAACGACAAGTTCGGTATCGTTGAAGCTCTGATGACCACTGTGCATGCAACTACCGCAACTCAGAAAACTGTTGA
TGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCATCCCAGAACATCATCCCTTCTTCTACCGGTGCTGCTAAAG
CAGTAGGTAAAGTTATCCCAGAACTGAACGGCAAACTGACCGGTATGGCGTTCCGCGTTCCAACTCCGAACGTGTCTGTG
GTTGACCTGACTGCTCGTCTGGCAAAACCAGCTTCTTACAAAGAAATCTGTGCTGCTATCAAAGCCGCTTCTGAAGGCGA
GCTGAAAGGCGTACTGGGTTACACCGAAGACGAAGTGGTTTCTACCGATTTCAACGGCGAAACTCTGACTTCCGTATTCG
ATGCCAAAGCCGGTATCGCTCTGAGCGACACCTTCGTGAAACTGGTTTCCTGGTACGACAACGAAACCGGCTATTCCAAC
AAGGTTCTGGATCTGATCGCTCACATCTCCAAATAA

Upstream 100 bases:

>100_bases
ATTTGCAGCAAGAATTGACACGATTCCGCTTGACGCTTGGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTA
ACAAATAGCTGGTGGAATAT

Downstream 100 bases:

>100_bases
TGGATTGATGTTAAGCTGATGATGAGGGTGACATTTTTGTCACCCTTTTTTTGTTTCTACGGCGAACAAAAGGCAACCCG
ATGAACGAAAAGATTTTCAC

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH-A [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTIKVGINGFGRIGRIVFRAAQERSDIEIVAINDLLDAEYMAYMLKYDSTHGRFNGTVEVKDGHLVVNGKTIRCTSERDP
ANLKWNEVNVDVVAEATGLFLTDETARKHIAAGAKKVVLTGPSKDDTPMFVMGVNHKSYAGQEIVSNASCTTNCLAPLAK
VINDKFGIVEALMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSV
VDLTARLAKPASYKEICAAIKAASEGELKGVLGYTEDEVVSTDFNGETLTSVFDAKAGIALSDTFVKLVSWYDNETGYSN
KVLDLIAHISK

Sequences:

>Translated_331_residues
MTIKVGINGFGRIGRIVFRAAQERSDIEIVAINDLLDAEYMAYMLKYDSTHGRFNGTVEVKDGHLVVNGKTIRCTSERDP
ANLKWNEVNVDVVAEATGLFLTDETARKHIAAGAKKVVLTGPSKDDTPMFVMGVNHKSYAGQEIVSNASCTTNCLAPLAK
VINDKFGIVEALMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSV
VDLTARLAKPASYKEICAAIKAASEGELKGVLGYTEDEVVSTDFNGETLTSVFDAKAGIALSDTFVKLVSWYDNETGYSN
KVLDLIAHISK
>Mature_330_residues
TIKVGINGFGRIGRIVFRAAQERSDIEIVAINDLLDAEYMAYMLKYDSTHGRFNGTVEVKDGHLVVNGKTIRCTSERDPA
NLKWNEVNVDVVAEATGLFLTDETARKHIAAGAKKVVLTGPSKDDTPMFVMGVNHKSYAGQEIVSNASCTTNCLAPLAKV
INDKFGIVEALMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVV
DLTARLAKPASYKEICAAIKAASEGELKGVLGYTEDEVVSTDFNGETLTSVFDAKAGIALSDTFVKLVSWYDNETGYSNK
VLDLIAHISK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=330, Percent_Identity=66.6666666666667, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI7657116, Length=330, Percent_Identity=63.6363636363636, Blast_Score=435, Evalue=1e-122,
Organism=Escherichia coli, GI1788079, Length=331, Percent_Identity=88.821752265861, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1789295, Length=329, Percent_Identity=41.3373860182371, Blast_Score=260, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI32566163, Length=334, Percent_Identity=64.6706586826347, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17568413, Length=334, Percent_Identity=64.6706586826347, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17534677, Length=334, Percent_Identity=63.1736526946108, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17534679, Length=334, Percent_Identity=62.5748502994012, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322409, Length=331, Percent_Identity=66.7673716012085, Blast_Score=453, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6321631, Length=331, Percent_Identity=66.7673716012085, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322468, Length=331, Percent_Identity=66.1631419939577, Blast_Score=449, Evalue=1e-127,
Organism=Drosophila melanogaster, GI85725000, Length=328, Percent_Identity=66.4634146341463, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI22023983, Length=328, Percent_Identity=66.4634146341463, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI17933600, Length=328, Percent_Identity=66.4634146341463, Blast_Score=440, Evalue=1e-124,
Organism=Drosophila melanogaster, GI18110149, Length=328, Percent_Identity=66.4634146341463, Blast_Score=440, Evalue=1e-124,
Organism=Drosophila melanogaster, GI19922412, Length=326, Percent_Identity=63.8036809815951, Blast_Score=421, Evalue=1e-118,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35455; Mature: 35324

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKVGINGFGRIGRIVFRAAQERSDIEIVAINDLLDAEYMAYMLKYDSTHGRFNGTVEV
CEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEECCEEEE
KDGHLVVNGKTIRCTSERDPANLKWNEVNVDVVAEATGLFLTDETARKHIAAGAKKVVLT
ECCEEEECCEEEEECCCCCCCCEEEEEEEEEEEEECCCEEEECHHHHHHHHCCCCEEEEE
GPSKDDTPMFVMGVNHKSYAGQEIVSNASCTTNCLAPLAKVINDKFGIVEALMTTVHATT
CCCCCCCCEEEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSV
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCEE
VDLTARLAKPASYKEICAAIKAASEGELKGVLGYTEDEVVSTDFNGETLTSVFDAKAGIA
EEEHHHHCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCHHHHHHHCCCCCE
LSDTFVKLVSWYDNETGYSNKVLDLIAHISK
EHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TIKVGINGFGRIGRIVFRAAQERSDIEIVAINDLLDAEYMAYMLKYDSTHGRFNGTVEV
EEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEECCEEEE
KDGHLVVNGKTIRCTSERDPANLKWNEVNVDVVAEATGLFLTDETARKHIAAGAKKVVLT
ECCEEEECCEEEEECCCCCCCCEEEEEEEEEEEEECCCEEEECHHHHHHHHCCCCEEEEE
GPSKDDTPMFVMGVNHKSYAGQEIVSNASCTTNCLAPLAKVINDKFGIVEALMTTVHATT
CCCCCCCCEEEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSV
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCEE
VDLTARLAKPASYKEICAAIKAASEGELKGVLGYTEDEVVSTDFNGETLTSVFDAKAGIA
EEEHHHHCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCHHHHHHHCCCCCE
LSDTFVKLVSWYDNETGYSNKVLDLIAHISK
EHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]