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The map label for this gene is purR [H]

Identifier: 157370919

GI number: 157370919

Start: 2948906

End: 2949931

Strand: Direct

Name: purR [H]

Synonym: Spro_2679

Alternate gene names: 157370919

Gene position: 2948906-2949931 (Clockwise)

Preceding gene: 157370918

Following gene: 157370924

Centisome position: 54.12

GC content: 45.91

Gene sequence:

>1026_bases
ATGGCTACCCTTAAAGACGTTGCACTCCATGCCGGTGTCTCTGTAACGACAGTATCGAATTTTATTAATAATAAAAAAAA
GCTCAAAGATGATACCCGGCATAACATTCTGGAGGCTATTCAAGCTACCGGCTACCGACACAATGCCCTGGCCGCCTCAT
TGAAGAAAAGCTCTGCGAGCCTGAGAAGCATAGGCATCATTTCCATCGTTGACCAAAATCCCTTTTTCTCCGAGTTATTC
TTCAAGCTGGAGAATGAGTGCTTCAAGCAAGACTTTACCGTTATATCCTGCTTCCGCCATAAAGATAAAGAGGATTTACA
CACCTACATTAATCTGATGTCGGGTCGTGTTGACGGAATCATCCTCATATCGTTAAAACGCGATAAAATCGATAACGTAA
TCAAACATATCCATGCGGTTCCCGTCGTTGCCATCGCTTTCGATATCGGTGATGTAATGAGCCTGTGCGGCGGTACGGAA
TTCAATTTACACAATGAGACCGGCGGCTACATAGCCGGCAGATTTTTGCTGTCCAAAGGGCACAAAAAAATTGCCTGCGT
TACCGGCCCAAAAGCGTTAAAAACCACGGTGAATCGCATAGCGGGTTTGAAAAAGGCACTTGCAGATTTTAATCTGCCGG
AAGACTCAGTCGATTTTATCGAAGGGAATTACACCTATCAGAGTGGCGTTGATGCCATGTATCAACTATTTTCCTCCGTG
GCGTTGCCAACGGCCATTATCTGTCATAACGACTTAATGGCCATTGGCGTACAAAATGCAGCGAATGAGTTAGGTTTGAA
AATACCCAGAGAACTCTCGGTCATTGGCTATGATGATATAGAAATGTCCAAATTGTCTTTCCCGTCACTCACCACGGTCA
GAATTCCTTTGGACGACATTGCTTGCCAGGCCCTGGAAGGGTTGAGCATGAAAATGGCCACCCCAGGCAGTAAAGCAATA
ATATCCGCCACGCCCAGCCTGGTTGTCAGGGGCTCAGTCAGTGAACCCACCGATTTTGGCAAATGA

Upstream 100 bases:

>100_bases
ACCGGGTACCCTCACGACCTGGGGCGCCTGTGGGTATTCTGTTAAGGGATGTTCACTATCAATCCATCTTGTGACATAAA
ACTGAGGTTATAAGTAGAGA

Downstream 100 bases:

>100_bases
CCATCAAAAACGCGTAGTCTAAGCGGTAACCCTCTCCGCCATGGTCGCCATATAGAGATCAAGCACGCCCCGATGGTCAA
CCTGAACACAGACCCGTTGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MATLKDVALHAGVSVTTVSNFINNKKKLKDDTRHNILEAIQATGYRHNALAASLKKSSASLRSIGIISIVDQNPFFSELF
FKLENECFKQDFTVISCFRHKDKEDLHTYINLMSGRVDGIILISLKRDKIDNVIKHIHAVPVVAIAFDIGDVMSLCGGTE
FNLHNETGGYIAGRFLLSKGHKKIACVTGPKALKTTVNRIAGLKKALADFNLPEDSVDFIEGNYTYQSGVDAMYQLFSSV
ALPTAIICHNDLMAIGVQNAANELGLKIPRELSVIGYDDIEMSKLSFPSLTTVRIPLDDIACQALEGLSMKMATPGSKAI
ISATPSLVVRGSVSEPTDFGK

Sequences:

>Translated_341_residues
MATLKDVALHAGVSVTTVSNFINNKKKLKDDTRHNILEAIQATGYRHNALAASLKKSSASLRSIGIISIVDQNPFFSELF
FKLENECFKQDFTVISCFRHKDKEDLHTYINLMSGRVDGIILISLKRDKIDNVIKHIHAVPVVAIAFDIGDVMSLCGGTE
FNLHNETGGYIAGRFLLSKGHKKIACVTGPKALKTTVNRIAGLKKALADFNLPEDSVDFIEGNYTYQSGVDAMYQLFSSV
ALPTAIICHNDLMAIGVQNAANELGLKIPRELSVIGYDDIEMSKLSFPSLTTVRIPLDDIACQALEGLSMKMATPGSKAI
ISATPSLVVRGSVSEPTDFGK
>Mature_340_residues
ATLKDVALHAGVSVTTVSNFINNKKKLKDDTRHNILEAIQATGYRHNALAASLKKSSASLRSIGIISIVDQNPFFSELFF
KLENECFKQDFTVISCFRHKDKEDLHTYINLMSGRVDGIILISLKRDKIDNVIKHIHAVPVVAIAFDIGDVMSLCGGTEF
NLHNETGGYIAGRFLLSKGHKKIACVTGPKALKTTVNRIAGLKKALADFNLPEDSVDFIEGNYTYQSGVDAMYQLFSSVA
LPTAIICHNDLMAIGVQNAANELGLKIPRELSVIGYDDIEMSKLSFPSLTTVRIPLDDIACQALEGLSMKMATPGSKAII
SATPSLVVRGSVSEPTDFGK

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=344, Percent_Identity=32.8488372093023, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1790194, Length=338, Percent_Identity=33.1360946745562, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=30.1775147928994, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1789068, Length=310, Percent_Identity=29.3548387096774, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=29.073482428115, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789202, Length=341, Percent_Identity=28.1524926686217, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1788474, Length=338, Percent_Identity=29.8816568047337, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786540, Length=297, Percent_Identity=27.9461279461279, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI48994940, Length=308, Percent_Identity=25.3246753246753, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787906, Length=303, Percent_Identity=26.4026402640264, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790715, Length=313, Percent_Identity=24.6006389776358, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37105; Mature: 36974

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLKDVALHAGVSVTTVSNFINNKKKLKDDTRHNILEAIQATGYRHNALAASLKKSSAS
CCCHHHHHHHCCCHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LRSIGIISIVDQNPFFSELFFKLENECFKQDFTVISCFRHKDKEDLHTYINLMSGRVDGI
HHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEE
ILISLKRDKIDNVIKHIHAVPVVAIAFDIGDVMSLCGGTEFNLHNETGGYIAGRFLLSKG
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHHCC
HKKIACVTGPKALKTTVNRIAGLKKALADFNLPEDSVDFIEGNYTYQSGVDAMYQLFSSV
CCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCHHHHHHHHHHHHHHH
ALPTAIICHNDLMAIGVQNAANELGLKIPRELSVIGYDDIEMSKLSFPSLTTVRIPLDDI
HHCEEEEECCCCEEEECHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCEEEEEECHHHH
ACQALEGLSMKMATPGSKAIISATPSLVVRGSVSEPTDFGK
HHHHHHCCEEEECCCCCCEEEECCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
ATLKDVALHAGVSVTTVSNFINNKKKLKDDTRHNILEAIQATGYRHNALAASLKKSSAS
CCHHHHHHHCCCHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LRSIGIISIVDQNPFFSELFFKLENECFKQDFTVISCFRHKDKEDLHTYINLMSGRVDGI
HHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEE
ILISLKRDKIDNVIKHIHAVPVVAIAFDIGDVMSLCGGTEFNLHNETGGYIAGRFLLSKG
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHHCC
HKKIACVTGPKALKTTVNRIAGLKKALADFNLPEDSVDFIEGNYTYQSGVDAMYQLFSSV
CCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCHHHHHHHHHHHHHHH
ALPTAIICHNDLMAIGVQNAANELGLKIPRELSVIGYDDIEMSKLSFPSLTTVRIPLDDI
HHCEEEEECCCCEEEECHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCEEEEEECHHHH
ACQALEGLSMKMATPGSKAIISATPSLVVRGSVSEPTDFGK
HHHHHHCCEEEECCCCCCEEEECCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]