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The map label for this gene is braC [H]

Identifier: 157370793

GI number: 157370793

Start: 2808643

End: 2809803

Strand: Direct

Name: braC [H]

Synonym: Spro_2553

Alternate gene names: 157370793

Gene position: 2808643-2809803 (Clockwise)

Preceding gene: 157370792

Following gene: 157370794

Centisome position: 51.55

GC content: 61.24

Gene sequence:

>1161_bases
ATGAGCAAGATAACTCGCTTCAAGCTGTTGGCGCTGGCCGCCGCCGTTCTGGTCAGCCTGGGCGCGACATCGCCGGTTCA
GGCCGATGAAACTATTTTGATCGGGCTGGCAGGCCCGCTGACCGGCCCTTCCGCCCGTATCGGCAAAGATTTGGAAAATG
GCGCGCAGTTGGCCATCGCCGATGCCAACGCCCAGAAACCGACGCTGAAAGGCAAGCCGGTCACCTTTAAACTGCTGTCC
GAGGACGATCAGTCAGACCCACGCACCGCCGTTGCGGTAGCCCAACGCCTGGTGGACGAAGGCGTTGCCGGCGTGGTCGG
CCATTGGAACACCGGCACCAGCATCCCGGCGGCGCGTATTTACCACGACGCCGGGATTGCTCAGGTCGCACCGGTCGCCA
CTGGCCACGGCTATACTCAGCAAGGGTTCGATACCAGCTTCCGCGTAATGGGCCACGACGATGACGGCGGTCATTATGCC
GGCGACTATGCGGTGAAGGTGCTGAAGGCCAAGCGTATTGCGGTAATCGACGATCGCACCGCGTTCGGCCAGGGGCTGGC
AGACGAGTTTATCAAGTCGTTGGCGGCTCAGGGCCTGCAACCGGTCGCGCGGGAGTATGTCGACGACAAGACCGTTGATT
TCAGTGCGGTATTGACCACCGTGCGCAGCAAGAACGCCGATTTGATCTTCTTCGGCGGCGTCGACTCGCAGGCCGCCCCG
CTGGCCCGACGCATCAAACAGTTGGGGATGAACACCCAACTGATGGGCGCAGGTGGCTTTGTCAGCCAGACTTTCCTGAC
GCTGGCACAGAAAGAAGGTGAAGGCGTGGTGGCACTGGAGCCGGGCCTGCCGCTGGAACAGATGCCAGGCGGCAAAGCCT
TTGAGCAGGCCTACCGTGACCGCTACAAAACCCATATCGAACTGCATGCGCCCTTCGCCTACGACGCTACGCGGGTATTG
ATTGCCGCCATCGAGCAGGCGGGGTCCGCCGATCCGGCCGATTATCTGCCGAAGCTGCGCGCCATCCATTATCAGGGCGT
CACAGGCACTATTGCGTTCGATGCACAGGGCAATCTGCAGCAGCCGAGTTTCACTCTTTACCGGGTGGTTGACGGCAAAT
GGCAGCCGCAAAGCGTACTGGGCGGCGCAAAAGCACAATAA

Upstream 100 bases:

>100_bases
CACTGTGGTCGCTCTGCCGACCTGCTGGACGATGAAAAAATCAAACAAGCCTATCTGGGCGAATAACCGCTTTTACTTTT
AACCACACCGGGAGTTTTAC

Downstream 100 bases:

>100_bases
CAATCCGCAAGGCAGTGAGGAGCAAGGCAATGAGCGAGGCAAAAAACGAATTGGGCATTCTGGCCCGACGCCGGATAGAG
GCGGAAATCATCAAGCCAAT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSKITRFKLLALAAAVLVSLGATSPVQADETILIGLAGPLTGPSARIGKDLENGAQLAIADANAQKPTLKGKPVTFKLLS
EDDQSDPRTAVAVAQRLVDEGVAGVVGHWNTGTSIPAARIYHDAGIAQVAPVATGHGYTQQGFDTSFRVMGHDDDGGHYA
GDYAVKVLKAKRIAVIDDRTAFGQGLADEFIKSLAAQGLQPVAREYVDDKTVDFSAVLTTVRSKNADLIFFGGVDSQAAP
LARRIKQLGMNTQLMGAGGFVSQTFLTLAQKEGEGVVALEPGLPLEQMPGGKAFEQAYRDRYKTHIELHAPFAYDATRVL
IAAIEQAGSADPADYLPKLRAIHYQGVTGTIAFDAQGNLQQPSFTLYRVVDGKWQPQSVLGGAKAQ

Sequences:

>Translated_386_residues
MSKITRFKLLALAAAVLVSLGATSPVQADETILIGLAGPLTGPSARIGKDLENGAQLAIADANAQKPTLKGKPVTFKLLS
EDDQSDPRTAVAVAQRLVDEGVAGVVGHWNTGTSIPAARIYHDAGIAQVAPVATGHGYTQQGFDTSFRVMGHDDDGGHYA
GDYAVKVLKAKRIAVIDDRTAFGQGLADEFIKSLAAQGLQPVAREYVDDKTVDFSAVLTTVRSKNADLIFFGGVDSQAAP
LARRIKQLGMNTQLMGAGGFVSQTFLTLAQKEGEGVVALEPGLPLEQMPGGKAFEQAYRDRYKTHIELHAPFAYDATRVL
IAAIEQAGSADPADYLPKLRAIHYQGVTGTIAFDAQGNLQQPSFTLYRVVDGKWQPQSVLGGAKAQ
>Mature_385_residues
SKITRFKLLALAAAVLVSLGATSPVQADETILIGLAGPLTGPSARIGKDLENGAQLAIADANAQKPTLKGKPVTFKLLSE
DDQSDPRTAVAVAQRLVDEGVAGVVGHWNTGTSIPAARIYHDAGIAQVAPVATGHGYTQQGFDTSFRVMGHDDDGGHYAG
DYAVKVLKAKRIAVIDDRTAFGQGLADEFIKSLAAQGLQPVAREYVDDKTVDFSAVLTTVRSKNADLIFFGGVDSQAAPL
ARRIKQLGMNTQLMGAGGFVSQTFLTLAQKEGEGVVALEPGLPLEQMPGGKAFEQAYRDRYKTHIELHAPFAYDATRVLI
AAIEQAGSADPADYLPKLRAIHYQGVTGTIAFDAQGNLQQPSFTLYRVVDGKWQPQSVLGGAKAQ

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=353, Percent_Identity=30.3116147308782, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI48994941, Length=338, Percent_Identity=29.585798816568, Blast_Score=158, Evalue=7e-40,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40898; Mature: 40767

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKITRFKLLALAAAVLVSLGATSPVQADETILIGLAGPLTGPSARIGKDLENGAQLAIA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHCCCCCCCCEEEEE
DANAQKPTLKGKPVTFKLLSEDDQSDPRTAVAVAQRLVDEGVAGVVGHWNTGTSIPAARI
ECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
YHDAGIAQVAPVATGHGYTQQGFDTSFRVMGHDDDGGHYAGDYAVKVLKAKRIAVIDDRT
HHHCCCHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCEECHHHHHHHHHHCEEEEEECCH
AFGQGLADEFIKSLAAQGLQPVAREYVDDKTVDFSAVLTTVRSKNADLIFFGGVDSQAAP
HHCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHH
LARRIKQLGMNTQLMGAGGFVSQTFLTLAQKEGEGVVALEPGLPLEQMPGGKAFEQAYRD
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHCCCCHHHHHHHHH
RYKTHIELHAPFAYDATRVLIAAIEQAGSADPADYLPKLRAIHYQGVTGTIAFDAQGNLQ
HHHEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEECCCEEEEEECCCCCCC
QPSFTLYRVVDGKWQPQSVLGGAKAQ
CCCEEEEEEECCCCCCHHHCCCCCCC
>Mature Secondary Structure 
SKITRFKLLALAAAVLVSLGATSPVQADETILIGLAGPLTGPSARIGKDLENGAQLAIA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHCCCCCCCCEEEEE
DANAQKPTLKGKPVTFKLLSEDDQSDPRTAVAVAQRLVDEGVAGVVGHWNTGTSIPAARI
ECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
YHDAGIAQVAPVATGHGYTQQGFDTSFRVMGHDDDGGHYAGDYAVKVLKAKRIAVIDDRT
HHHCCCHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCEECHHHHHHHHHHCEEEEEECCH
AFGQGLADEFIKSLAAQGLQPVAREYVDDKTVDFSAVLTTVRSKNADLIFFGGVDSQAAP
HHCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHH
LARRIKQLGMNTQLMGAGGFVSQTFLTLAQKEGEGVVALEPGLPLEQMPGGKAFEQAYRD
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHCCCCHHHHHHHHH
RYKTHIELHAPFAYDATRVLIAAIEQAGSADPADYLPKLRAIHYQGVTGTIAFDAQGNLQ
HHHEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEECCCEEEEEECCCCCCC
QPSFTLYRVVDGKWQPQSVLGGAKAQ
CCCEEEEEEECCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]