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The map label for this gene is gdhA [H]

Identifier: 157370713

GI number: 157370713

Start: 2710219

End: 2711493

Strand: Direct

Name: gdhA [H]

Synonym: Spro_2473

Alternate gene names: 157370713

Gene position: 2710219-2711493 (Clockwise)

Preceding gene: 157370710

Following gene: 157370715

Centisome position: 49.74

GC content: 56.78

Gene sequence:

>1275_bases
ATGGAAAAGCTATCCTACGCTTCAGAAAGCAGCACAACGGCCTGGGCCACCTATCTGCAACAAATCGACCGCGTTGCACC
TTACCTGGGTGATCTGTCGCGTTGGGTTGATACCCTGCGTCACCCGAAGCGTGCGCTGATCGTTGATATCCCACTGCAGA
TGGACGACGGTTCAATCCGCCATTTCGAAGGCTTCCGCGTTCAGCACAACCTGTCGCGCGGCCCGGGTAAAGGCGGCATC
CGTTATCACCCGGACGTTGATCTCGATGAAGTCATGGCGCTGTCAGCCTGGATGACCATCAAATGTGCGGCAGTCAACCT
GCCCTACGGTGGCGCCAAGGGGGGGATCCGCGTCGATCCTTTCAAACTGTCTGAAGGCGAGCTGGAAAGATTGACCCGTC
GCTACACCAGCGAAATCGGTTTTATCATCGGGCCACAGAAAGATATCCCGGCACCGGACGTCGGCACCAACTCCAAAGTC
ATGGCCTGGATGATGGATACCTACTCCATGAACCAGGGCACTACCATCACCGGCGTGGTTACCGGCAAACCTATTCACCT
GGGCGGCTCTCTGGGTCGTGAGAAAGCCACCGGTCGCGGTGTATTCGTTACCGGCTGTGAAGTGGCGAAACGTCTGGGCG
TGCAAATCGAAGGTGCCAAGGTTGCGGTTCAGGGCTTTGGTAACGTGGGCAGCGAAGCCGCCCGCCTGTTTGTTGGTATC
GGCGCGCGCGTAGTGGTGATTCAGGACCACTCCGCTACCCTGTTCAACGCCAACGGCATCGATTTGACTGCACTGACCGA
ATGGCAGGCCAAGAACAAGCAAATCGCCGGCTTCCCTGGCGCCAGCGAAATCGAAAGCGAAGCTTTCTGGTCCGTAGACA
TGGATATCCTGATCCCAGCCGCACTGGAAGGTCAGATTACCCGTCAACGTGCAGAAATCCTCAGCGCCAAACTGGTTCTG
GAAGGCGCTAACGGCCCAACCTACCCAGATGCGGACGACGTGCTGCGTTCACGCAACATCACCGTGGTACCTGACGTTAT
CTGTAACGCCGGCGGCGTAACGGTCAGCTACTTCGAATGGGTGCAGGACATGGCCAGCTACTTCTGGAGCGAAAGCGAAA
TCAACGAGCGTATGGACAAGATCATGACCGACGCCATGATTCACGTTTGGAACAAGTCCGAAGAGAAAGAGTGCAGCCTG
CGCACAGCGGCTTATATCGTCGCCTGTGAGCGAATCCTGACCGCACGTAAAGAGCGTGGCATTTACCCAGGTTAA

Upstream 100 bases:

>100_bases
AGCGACACTTTTAATGGTAAATTTGGCGTCGCAAAAGTGTTATGCACTTGGTAAACCACGGTTAAACAAAATAAACACCT
CTATATAAAAAAGGTTGGTT

Downstream 100 bases:

>100_bases
TGCGTTATCTCTCCCGGCTTGCTCTGCAGGCCGGGATTATTTCAAAGCGATCCCCTTCAACGCAGCAATCGTTTCATCAC
CTTGCCGGTACTGGTCATCG

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MEKLSYASESSTTAWATYLQQIDRVAPYLGDLSRWVDTLRHPKRALIVDIPLQMDDGSIRHFEGFRVQHNLSRGPGKGGI
RYHPDVDLDEVMALSAWMTIKCAAVNLPYGGAKGGIRVDPFKLSEGELERLTRRYTSEIGFIIGPQKDIPAPDVGTNSKV
MAWMMDTYSMNQGTTITGVVTGKPIHLGGSLGREKATGRGVFVTGCEVAKRLGVQIEGAKVAVQGFGNVGSEAARLFVGI
GARVVVIQDHSATLFNANGIDLTALTEWQAKNKQIAGFPGASEIESEAFWSVDMDILIPAALEGQITRQRAEILSAKLVL
EGANGPTYPDADDVLRSRNITVVPDVICNAGGVTVSYFEWVQDMASYFWSESEINERMDKIMTDAMIHVWNKSEEKECSL
RTAAYIVACERILTARKERGIYPG

Sequences:

>Translated_424_residues
MEKLSYASESSTTAWATYLQQIDRVAPYLGDLSRWVDTLRHPKRALIVDIPLQMDDGSIRHFEGFRVQHNLSRGPGKGGI
RYHPDVDLDEVMALSAWMTIKCAAVNLPYGGAKGGIRVDPFKLSEGELERLTRRYTSEIGFIIGPQKDIPAPDVGTNSKV
MAWMMDTYSMNQGTTITGVVTGKPIHLGGSLGREKATGRGVFVTGCEVAKRLGVQIEGAKVAVQGFGNVGSEAARLFVGI
GARVVVIQDHSATLFNANGIDLTALTEWQAKNKQIAGFPGASEIESEAFWSVDMDILIPAALEGQITRQRAEILSAKLVL
EGANGPTYPDADDVLRSRNITVVPDVICNAGGVTVSYFEWVQDMASYFWSESEINERMDKIMTDAMIHVWNKSEEKECSL
RTAAYIVACERILTARKERGIYPG
>Mature_424_residues
MEKLSYASESSTTAWATYLQQIDRVAPYLGDLSRWVDTLRHPKRALIVDIPLQMDDGSIRHFEGFRVQHNLSRGPGKGGI
RYHPDVDLDEVMALSAWMTIKCAAVNLPYGGAKGGIRVDPFKLSEGELERLTRRYTSEIGFIIGPQKDIPAPDVGTNSKV
MAWMMDTYSMNQGTTITGVVTGKPIHLGGSLGREKATGRGVFVTGCEVAKRLGVQIEGAKVAVQGFGNVGSEAARLFVGI
GARVVVIQDHSATLFNANGIDLTALTEWQAKNKQIAGFPGASEIESEAFWSVDMDILIPAALEGQITRQRAEILSAKLVL
EGANGPTYPDADDVLRSRNITVVPDVICNAGGVTVSYFEWVQDMASYFWSESEINERMDKIMTDAMIHVWNKSEEKECSL
RTAAYIVACERILTARKERGIYPG

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=333, Percent_Identity=44.7447447447448, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI4885281, Length=333, Percent_Identity=44.1441441441441, Blast_Score=277, Evalue=2e-74,
Organism=Escherichia coli, GI1788059, Length=429, Percent_Identity=32.1678321678322, Blast_Score=175, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17544676, Length=376, Percent_Identity=39.0957446808511, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6319256, Length=383, Percent_Identity=28.4595300261097, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324951, Length=380, Percent_Identity=28.6842105263158, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45549226, Length=351, Percent_Identity=43.3048433048433, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI45553475, Length=351, Percent_Identity=43.3048433048433, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24649283, Length=330, Percent_Identity=44.2424242424242, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24649460, Length=324, Percent_Identity=40.7407407407407, Blast_Score=228, Evalue=6e-60,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46465; Mature: 46465

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSYASESSTTAWATYLQQIDRVAPYLGDLSRWVDTLRHPKRALIVDIPLQMDDGSIR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCEE
HFEGFRVQHNLSRGPGKGGIRYHPDVDLDEVMALSAWMTIKCAAVNLPYGGAKGGIRVDP
EECCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHCEEEEEEEEECCCCCCCCCEEECC
FKLSEGELERLTRRYTSEIGFIIGPQKDIPAPDVGTNSKVMAWMMDTYSMNQGTTITGVV
EECCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEEEEEEEHEECCCCCEEEEEE
TGKPIHLGGSLGREKATGRGVFVTGCEVAKRLGVQIEGAKVAVQGFGNVGSEAARLFVGI
ECCEEEECCCCCCCCCCCCEEEEECHHHHHHCCCEECCCEEEEEECCCCCHHHHHEEEEC
GARVVVIQDHSATLFNANGIDLTALTEWQAKNKQIAGFPGASEIESEAFWSVDMDILIPA
CEEEEEEECCCEEEEECCCCEEEEEECCCCCCCEEECCCCCHHHCCCCEEEECHHEEECC
ALEGQITRQRAEILSAKLVLEGANGPTYPDADDVLRSRNITVVPDVICNAGGVTVSYFEW
HHCCHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHCCCCEEECHHHCCCCCCCHHHHHH
VQDMASYFWSESEINERMDKIMTDAMIHVWNKSEEKECSLRTAAYIVACERILTARKERG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
IYPG
CCCC
>Mature Secondary Structure
MEKLSYASESSTTAWATYLQQIDRVAPYLGDLSRWVDTLRHPKRALIVDIPLQMDDGSIR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCEE
HFEGFRVQHNLSRGPGKGGIRYHPDVDLDEVMALSAWMTIKCAAVNLPYGGAKGGIRVDP
EECCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHCEEEEEEEEECCCCCCCCCEEECC
FKLSEGELERLTRRYTSEIGFIIGPQKDIPAPDVGTNSKVMAWMMDTYSMNQGTTITGVV
EECCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEEEEEEEHEECCCCCEEEEEE
TGKPIHLGGSLGREKATGRGVFVTGCEVAKRLGVQIEGAKVAVQGFGNVGSEAARLFVGI
ECCEEEECCCCCCCCCCCCEEEEECHHHHHHCCCEECCCEEEEEECCCCCHHHHHEEEEC
GARVVVIQDHSATLFNANGIDLTALTEWQAKNKQIAGFPGASEIESEAFWSVDMDILIPA
CEEEEEEECCCEEEEECCCCEEEEEECCCCCCCEEECCCCCHHHCCCCEEEECHHEEECC
ALEGQITRQRAEILSAKLVLEGANGPTYPDADDVLRSRNITVVPDVICNAGGVTVSYFEW
HHCCHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHCCCCEEECHHHCCCCCCCHHHHHH
VQDMASYFWSESEINERMDKIMTDAMIHVWNKSEEKECSLRTAAYIVACERILTARKERG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
IYPG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]