Click here to switch to the map view.

The map label for this gene is metG

Identifier: 157369816

GI number: 157369816

Start: 1714154

End: 1716181

Strand: Reverse

Name: metG

Synonym: Spro_1573

Alternate gene names: 157369816

Gene position: 1716181-1714154 (Counterclockwise)

Preceding gene: 157369818

Following gene: 157369814

Centisome position: 31.5

GC content: 54.83

Gene sequence:

>2028_bases
ATGCCTCAAGTCGCGAAAAAATTATTGGTGACGTGCGCGCTGCCGTACGCAAATGGTTCCATCCATCTCGGCCACATGCT
CGAGCACATCCAGGCAGACATCTGGGTTCGTTACCAACGAATGCGCGGCAACGAGGTTCATTTCATCTGCGCGGACGACG
CGCACGGCACGCCGATCATGCTGAAAGCTCAGCAGTTGGGTATCAAGCCGGAAGAAATGATCGCGGAAATGAGCCAGGAG
CACCAACAGGATTTCGCCGGTTTTGGCATCAGCTATGATAACTATCATTCAACCCATAGCGATGAGAACCGTGAGCTGTC
GAACCTGATTTACGGCCGCCTGAAAGAAAACGGTTTCATCAAGAACCGCACCATTTCTCAGCTGTACGACCCGGAAAAAG
GTATGTTCCTGCCTGACCGCTTCGTCAAAGGCACCTGCCCGAAATGCAAATCACCGGATCAATACGGCGATAACTGTGAA
GTCTGTGGCGCAACCTATAGTCCGACCGAGCTGATCGATCCGAAATCCGTGGTTTCCGGTGCGACGCCGGTGCTGCGTGA
TTCCGAGCACTTCTTCTTTGATCTGCCGTCGTTCAGCGAAATGCTGCAGGCCTGGACCCGTTCCGGCGCGCTGCAAGAGC
AGGTCGCAAACAAGATGCAGGAATGGTTTGAATCCGGTCTGCAACAGTGGGATATCTCTCGCGATGCGCCGTATTTCGGC
TTTGAAATCCCGGATGCGCCAGGTAAATACTTCTATGTCTGGCTGGATGCGCCAATCGGCTACATGGGGTCATTCAAAAA
CCTATGCGACAAGCGTGGCGATCTGGACTTTGACGAGTTCTGGCGCAAGGACTCCACCACCGAGCTGTATCACTTTATCG
GCAAGGACATCGTTTATTTCCACAGCCTGTTCTGGCCGGCAATGCTGGAAGGCAGTAACTTCCGCAAACCGACCAACCTG
TTCGTACATGGCTACGTAACGGTAAACGGCGCCAAGATGTCCAAATCTCGCGGCACCTTTATCAAGGCCGGTACCTATCT
GCAACACCTGGATGCCGATTGCCTGCGTTACTACTACGCCGCCAAGTTGTCTTCACGCATTGACGATATCGACCTGAATC
TGGAAGATTTCGTGCAGCGGGTAAACGCCGACATCGTGAACAAGGTGGTGAACCTGGCTTCGCGCAACGCCGGCTTTATC
AGCAAGCGTTTTGGTGGTCAACTGGCCGACAAGCTGGCCGATCCGGCGCTGTACCAAACCTTCGTTGATGCAGCGCAAAG
CATCGCCGAGGCCTATGCCAGCCGTGAATCCGGCCGGGCAATTCGCGAAATCATGGCGCTGGCCGATCTGGCAAACCGCT
ATGTGGATGAACAGGCACCATGGGTAGTGGCCAAACAGGAAGGCCGCGATGCGGATTTACAAGCCATTTGCTCAATGGGC
ATCAACCTGTTCCGCGTCCTGATGACCTACCTGAAACCGGTAATGCCGTCGCTGACTGAACGCACCGAAGCCTTCCTTAA
CTGTGAGCTGAGCTGGGACAGCATCCAGCAGCCGTTGCTGGGCCATCAGGTTAATCCATTCAAAGCCCTGTTCAACCGTA
TCGATCTGGACAAGGTCAATGAGATGGTCAGCGCCTCGAAAGAAGACATGGTGGCGGCGAAAGTGGTCACCGGCCCACTG
GCTGAGGATCCGATTCAGGACACCATTACCTTTGACGATTTCGCCAAGGTGGATATGCGCATTGCGCTGATCACCAGCGC
CGACTTTGTTGATGGTTCGGACAAACTGCTGAAATTGCAGTTGGATCTGGGCGGTGAAACGCGTCAGATCTTCTCCGGTA
TTCGTTCCGCTTACCCGGACCCGAAAGCGCTGGAAGGCCGCCTGACCATCATGGTTGCCAACCTTGCGCCACGCAAAATG
CGCTTCGGTATCTCCGAAGGCATGGTGATGGCTGCCGGCCCGGGCGGAAAAGAAATTTTCCTGCTTAGCCCGGACAGCGG
CGCACAGCCAGGGATGCAGGTGAAGTAA

Upstream 100 bases:

>100_bases
TCGGCGCATGTCTGTATAAAGAAACCACCCTAGCCGTGGCGAGCGCGATCGGGTAACATCAAAGACCCTTTTTATTCATT
ACAGAAACCAAGTTCTCACT

Downstream 100 bases:

>100_bases
GCTTCCTAACGAGTCCCCTCACCCTTACCCTCTCCCCAGGGAGAGGGGACCGTTATGCGATGCCATTCAGTATGATCTGG
CCTAATATTCTGCTCCCCCG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MPQVAKKLLVTCALPYANGSIHLGHMLEHIQADIWVRYQRMRGNEVHFICADDAHGTPIMLKAQQLGIKPEEMIAEMSQE
HQQDFAGFGISYDNYHSTHSDENRELSNLIYGRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
VCGATYSPTELIDPKSVVSGATPVLRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANKMQEWFESGLQQWDISRDAPYFG
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCDKRGDLDFDEFWRKDSTTELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNL
FVHGYVTVNGAKMSKSRGTFIKAGTYLQHLDADCLRYYYAAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFI
SKRFGGQLADKLADPALYQTFVDAAQSIAEAYASRESGRAIREIMALADLANRYVDEQAPWVVAKQEGRDADLQAICSMG
INLFRVLMTYLKPVMPSLTERTEAFLNCELSWDSIQQPLLGHQVNPFKALFNRIDLDKVNEMVSASKEDMVAAKVVTGPL
AEDPIQDTITFDDFAKVDMRIALITSADFVDGSDKLLKLQLDLGGETRQIFSGIRSAYPDPKALEGRLTIMVANLAPRKM
RFGISEGMVMAAGPGGKEIFLLSPDSGAQPGMQVK

Sequences:

>Translated_675_residues
MPQVAKKLLVTCALPYANGSIHLGHMLEHIQADIWVRYQRMRGNEVHFICADDAHGTPIMLKAQQLGIKPEEMIAEMSQE
HQQDFAGFGISYDNYHSTHSDENRELSNLIYGRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
VCGATYSPTELIDPKSVVSGATPVLRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANKMQEWFESGLQQWDISRDAPYFG
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCDKRGDLDFDEFWRKDSTTELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNL
FVHGYVTVNGAKMSKSRGTFIKAGTYLQHLDADCLRYYYAAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFI
SKRFGGQLADKLADPALYQTFVDAAQSIAEAYASRESGRAIREIMALADLANRYVDEQAPWVVAKQEGRDADLQAICSMG
INLFRVLMTYLKPVMPSLTERTEAFLNCELSWDSIQQPLLGHQVNPFKALFNRIDLDKVNEMVSASKEDMVAAKVVTGPL
AEDPIQDTITFDDFAKVDMRIALITSADFVDGSDKLLKLQLDLGGETRQIFSGIRSAYPDPKALEGRLTIMVANLAPRKM
RFGISEGMVMAAGPGGKEIFLLSPDSGAQPGMQVK
>Mature_674_residues
PQVAKKLLVTCALPYANGSIHLGHMLEHIQADIWVRYQRMRGNEVHFICADDAHGTPIMLKAQQLGIKPEEMIAEMSQEH
QQDFAGFGISYDNYHSTHSDENRELSNLIYGRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCEV
CGATYSPTELIDPKSVVSGATPVLRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANKMQEWFESGLQQWDISRDAPYFGF
EIPDAPGKYFYVWLDAPIGYMGSFKNLCDKRGDLDFDEFWRKDSTTELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLF
VHGYVTVNGAKMSKSRGTFIKAGTYLQHLDADCLRYYYAAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFIS
KRFGGQLADKLADPALYQTFVDAAQSIAEAYASRESGRAIREIMALADLANRYVDEQAPWVVAKQEGRDADLQAICSMGI
NLFRVLMTYLKPVMPSLTERTEAFLNCELSWDSIQQPLLGHQVNPFKALFNRIDLDKVNEMVSASKEDMVAAKVVTGPLA
EDPIQDTITFDDFAKVDMRIALITSADFVDGSDKLLKLQLDLGGETRQIFSGIRSAYPDPKALEGRLTIMVANLAPRKMR
FGISEGMVMAAGPGGKEIFLLSPDSGAQPGMQVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=564, Percent_Identity=28.5460992907801, Blast_Score=247, Evalue=3e-65,
Organism=Homo sapiens, GI24308436, Length=564, Percent_Identity=25.354609929078, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1788432, Length=677, Percent_Identity=87.1491875923191, Blast_Score=1251, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=572, Percent_Identity=28.8461538461538, Blast_Score=241, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI212645096, Length=517, Percent_Identity=23.0174081237911, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71992781, Length=411, Percent_Identity=22.8710462287105, Blast_Score=108, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=556, Percent_Identity=28.2374100719424, Blast_Score=243, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6321610, Length=542, Percent_Identity=23.2472324723247, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24655311, Length=574, Percent_Identity=29.9651567944251, Blast_Score=238, Evalue=7e-63,
Organism=Drosophila melanogaster, GI21358483, Length=513, Percent_Identity=24.9512670565302, Blast_Score=135, Evalue=9e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_SERP5 (A8GC37)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477805.1
- ProteinModelPortal:   A8GC37
- STRING:   A8GC37
- GeneID:   5602898
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1573
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- BioCyc:   SPRO399741:SPRO_1573-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75837; Mature: 75706

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQVAKKLLVTCALPYANGSIHLGHMLEHIQADIWVRYQRMRGNEVHFICADDAHGTPIM
CCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
LKAQQLGIKPEEMIAEMSQEHQQDFAGFGISYDNYHSTHSDENRELSNLIYGRLKENGFI
EEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
KNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCEVCGATYSPTELIDPKSVVSG
CCCCHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHCC
ATPVLRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANKMQEWFESGLQQWDISRDAPYFG
CCCCEECCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCDKRGDLDFDEFWRKDSTTELYHFIGKDIVYF
EECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
HSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKMSKSRGTFIKAGTYLQHLDADCLRYYYA
HHHHHHHHHCCCCCCCCCEEEEEEEEEEECCEECCCCCCEEECCHHHHHHCHHHHHHHHH
AKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFISKRFGGQLADKLADPALYQT
HHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHCCHHHHHH
FVDAAQSIAEAYASRESGRAIREIMALADLANRYVDEQAPWVVAKQEGRDADLQAICSMG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
INLFRVLMTYLKPVMPSLTERTEAFLNCELSWDSIQQPLLGHQVNPFKALFNRIDLDKVN
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHCCHHCCCCCHHHHHHHHCCHHHHH
EMVSASKEDMVAAKVVTGPLAEDPIQDTITFDDFAKVDMRIALITSADFVDGSDKLLKLQ
HHHHCCHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHCCEEEEEEEECCCCCCCCCEEEEE
LDLGGETRQIFSGIRSAYPDPKALEGRLTIMVANLAPRKMRFGISEGMVMAAGPGGKEIF
ECCCCCHHHHHHHHHHCCCCCHHCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCCCEEE
LLSPDSGAQPGMQVK
EECCCCCCCCCCCCC
>Mature Secondary Structure 
PQVAKKLLVTCALPYANGSIHLGHMLEHIQADIWVRYQRMRGNEVHFICADDAHGTPIM
CHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
LKAQQLGIKPEEMIAEMSQEHQQDFAGFGISYDNYHSTHSDENRELSNLIYGRLKENGFI
EEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
KNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCEVCGATYSPTELIDPKSVVSG
CCCCHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHCC
ATPVLRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANKMQEWFESGLQQWDISRDAPYFG
CCCCEECCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCDKRGDLDFDEFWRKDSTTELYHFIGKDIVYF
EECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
HSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKMSKSRGTFIKAGTYLQHLDADCLRYYYA
HHHHHHHHHCCCCCCCCCEEEEEEEEEEECCEECCCCCCEEECCHHHHHHCHHHHHHHHH
AKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFISKRFGGQLADKLADPALYQT
HHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHCCHHHHHH
FVDAAQSIAEAYASRESGRAIREIMALADLANRYVDEQAPWVVAKQEGRDADLQAICSMG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
INLFRVLMTYLKPVMPSLTERTEAFLNCELSWDSIQQPLLGHQVNPFKALFNRIDLDKVN
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHCCHHCCCCCHHHHHHHHCCHHHHH
EMVSASKEDMVAAKVVTGPLAEDPIQDTITFDDFAKVDMRIALITSADFVDGSDKLLKLQ
HHHHCCHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHCCEEEEEEEECCCCCCCCCEEEEE
LDLGGETRQIFSGIRSAYPDPKALEGRLTIMVANLAPRKMRFGISEGMVMAAGPGGKEIF
ECCCCCHHHHHHHHHHCCCCCHHCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCCCEEE
LLSPDSGAQPGMQVK
EECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA