Click here to switch to the map view.

The map label for this gene is phoA [H]

Identifier: 157369771

GI number: 157369771

Start: 1666545

End: 1667972

Strand: Reverse

Name: phoA [H]

Synonym: Spro_1528

Alternate gene names: 157369771

Gene position: 1667972-1666545 (Counterclockwise)

Preceding gene: 157369772

Following gene: 157369770

Centisome position: 30.61

GC content: 57.84

Gene sequence:

>1428_bases
ATGCTGCAGCCAGTTTCTTTGATCGCCGGCGCCGTGTTATCCGCCTTACTCTGTTCCGCCGCCTTCGCCGATGAAACCCC
GGTCAATACCGCCAGCCTTTACGAACGTGCGGCACGCGGCAACATCGTGGAGCCCGGTGGCGCACGCCGGTTGAGTGGCG
ATCAAACGGCCGCGTTGAAGGCTTCTTTGTCTGACAAAACGGTCAAAAACGTGATTTTATTGATCGGCGATGGCATGGGT
GATTCGGAAATCACCTCGGCGCGTAACTACGCCGAAGGGGCGGGCGGCTATTTTAAAGGCATTGATGCCCTGCCGCTGAC
CGGTCAATACACCCATTACTCACTGGACAAGAAAACCCACAAACCGGATTACGTCACCGACTCCGCCGCCTCGGCGACCG
CATGGTCAACCGGTGTAAAAACCTATAACGGCGCGCTGGGCGTTGACGTCAATGGCAAAGACCAGCCGACACTGCTGGAA
ATCGCCAAGGCGGCGGGTAAAGCCACCGGGAATGTGTCCACCGCCGAACTGCAGGACGCCACGCCGGCGGCGCAAATTTC
CCATGTCACCTCGCGCAAATGTTACGGCCCAGAAGAAACCAGCGAAAAATGTGCCACCAACGCGCTGGAAAACGGCGGCC
GTGGCTCCATCAGTGAGCAGTTGCTGAAGGCTCGCGCCGACGTCACGCTGGGCGGTGGGGCAAAATCCTTTAATCAGCTG
GCAAAAAGCGGCGAATGGCAGGGAAAATCGCTAAAAGAACAGGCAGCCGCTCTGGGTTATCAGTGGGTCGAAAACCTCGA
TGCCCTGAACGCCGTCAGCGTGGCCAATCAGCAAAAACCGCTGCTCGGGCTGTTTGCCGCCGGTAATATGCCAGTGCGCT
GGCAAGGGCCGAAAGCCACTTATCACGGCAATATCGACCAGCCTGCGGTGACCTGTGAAAATAATCCGGCACGCACTGCG
GATATCCCGACGCTGGCGGCGATGACTGAAAAAGCCATCGACCTGCTGAAAACCAACCAAAACGGCTTCTTCCTGCAGAT
TGAAGGCGCGTCGATCGACAAACAGGATCATGCTGCCAACCCGTGCGGCCAGTTTGGTGAAACTGTCGATCTGGATGAAG
CGGTTCAGAAAGCCCTGGCATTCGCCCGCGCAGATGGCAACACCCTGGTGATAGTCACCGCCGACCATGCCCATTCCAGC
CAGATTATCGCCGCCGAAGCCAAGGCCCCAGGCCTGACGCAAACGCTGACCACCAAAGATGGCGCACCGATGACCATCAG
CTACGGCAATTCAGAAGAAGATTCGCAGGGGCACACCGGGACTCAATTGCGGGTGGCAGCTTACGGTCCACATGCCGCCA
ACGTGGTTGGCCTGACCGATCAGACGGATCTGTTCTATACCATGCGTGATGCGATGGCGATTAAGTAA

Upstream 100 bases:

>100_bases
CCCCAATTACCGTCATAAAAGTGTCACACCTCTGCACTACTCTCCCTGCGTAACAAACTGATGCTATTTATATTTTTTTA
TTACGACAAGGAGCTTTCCC

Downstream 100 bases:

>100_bases
GTTAATAACCTGGTAACTGATTAAGCCGACCCTATTCGTCCCTGCTCTTGCAAGTGATGAATAGGGTTTTCACCGCTTAG
CCCAAAACTGCTATATTGAA

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MLQPVSLIAGAVLSALLCSAAFADETPVNTASLYERAARGNIVEPGGARRLSGDQTAALKASLSDKTVKNVILLIGDGMG
DSEITSARNYAEGAGGYFKGIDALPLTGQYTHYSLDKKTHKPDYVTDSAASATAWSTGVKTYNGALGVDVNGKDQPTLLE
IAKAAGKATGNVSTAELQDATPAAQISHVTSRKCYGPEETSEKCATNALENGGRGSISEQLLKARADVTLGGGAKSFNQL
AKSGEWQGKSLKEQAAALGYQWVENLDALNAVSVANQQKPLLGLFAAGNMPVRWQGPKATYHGNIDQPAVTCENNPARTA
DIPTLAAMTEKAIDLLKTNQNGFFLQIEGASIDKQDHAANPCGQFGETVDLDEAVQKALAFARADGNTLVIVTADHAHSS
QIIAAEAKAPGLTQTLTTKDGAPMTISYGNSEEDSQGHTGTQLRVAAYGPHAANVVGLTDQTDLFYTMRDAMAIK

Sequences:

>Translated_475_residues
MLQPVSLIAGAVLSALLCSAAFADETPVNTASLYERAARGNIVEPGGARRLSGDQTAALKASLSDKTVKNVILLIGDGMG
DSEITSARNYAEGAGGYFKGIDALPLTGQYTHYSLDKKTHKPDYVTDSAASATAWSTGVKTYNGALGVDVNGKDQPTLLE
IAKAAGKATGNVSTAELQDATPAAQISHVTSRKCYGPEETSEKCATNALENGGRGSISEQLLKARADVTLGGGAKSFNQL
AKSGEWQGKSLKEQAAALGYQWVENLDALNAVSVANQQKPLLGLFAAGNMPVRWQGPKATYHGNIDQPAVTCENNPARTA
DIPTLAAMTEKAIDLLKTNQNGFFLQIEGASIDKQDHAANPCGQFGETVDLDEAVQKALAFARADGNTLVIVTADHAHSS
QIIAAEAKAPGLTQTLTTKDGAPMTISYGNSEEDSQGHTGTQLRVAAYGPHAANVVGLTDQTDLFYTMRDAMAIK
>Mature_475_residues
MLQPVSLIAGAVLSALLCSAAFADETPVNTASLYERAARGNIVEPGGARRLSGDQTAALKASLSDKTVKNVILLIGDGMG
DSEITSARNYAEGAGGYFKGIDALPLTGQYTHYSLDKKTHKPDYVTDSAASATAWSTGVKTYNGALGVDVNGKDQPTLLE
IAKAAGKATGNVSTAELQDATPAAQISHVTSRKCYGPEETSEKCATNALENGGRGSISEQLLKARADVTLGGGAKSFNQL
AKSGEWQGKSLKEQAAALGYQWVENLDALNAVSVANQQKPLLGLFAAGNMPVRWQGPKATYHGNIDQPAVTCENNPARTA
DIPTLAAMTEKAIDLLKTNQNGFFLQIEGASIDKQDHAANPCGQFGETVDLDEAVQKALAFARADGNTLVIVTADHAHSS
QIIAAEAKAPGLTQTLTTKDGAPMTISYGNSEEDSQGHTGTQLRVAAYGPHAANVVGLTDQTDLFYTMRDAMAIK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=488, Percent_Identity=28.8934426229508, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI94721246, Length=488, Percent_Identity=28.4836065573771, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI116734717, Length=406, Percent_Identity=29.5566502463054, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI157266292, Length=480, Percent_Identity=26.6666666666667, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI294660770, Length=401, Percent_Identity=28.6783042394015, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI48994877, Length=472, Percent_Identity=75.635593220339, Blast_Score=731, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320689, Length=344, Percent_Identity=32.5581395348837, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI21355151, Length=426, Percent_Identity=30.9859154929577, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI19921912, Length=394, Percent_Identity=31.9796954314721, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI21355981, Length=473, Percent_Identity=28.1183932346723, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355149, Length=426, Percent_Identity=27.4647887323944, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24651554, Length=500, Percent_Identity=27.2, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18859923, Length=365, Percent_Identity=30.1369863013699, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24585213, Length=426, Percent_Identity=28.8732394366197, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI85725200, Length=405, Percent_Identity=27.1604938271605, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI85815833, Length=405, Percent_Identity=27.1604938271605, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24657827, Length=404, Percent_Identity=29.7029702970297, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21358067, Length=394, Percent_Identity=28.4263959390863, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24657835, Length=401, Percent_Identity=26.9326683291771, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24657842, Length=394, Percent_Identity=27.1573604060914, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 49671; Mature: 49671

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQPVSLIAGAVLSALLCSAAFADETPVNTASLYERAARGNIVEPGGARRLSGDQTAALK
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
ASLSDKTVKNVILLIGDGMGDSEITSARNYAEGAGGYFKGIDALPLTGQYTHYSLDKKTH
CCCCHHCEEEEEEEEECCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEECCCCCC
KPDYVTDSAASATAWSTGVKTYNGALGVDVNGKDQPTLLEIAKAAGKATGNVSTAELQDA
CCCCCCCCCCCCCHHHCCCCEECCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHCCC
TPAAQISHVTSRKCYGPEETSEKCATNALENGGRGSISEQLLKARADVTLGGGAKSFNQL
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCHHHHHHH
AKSGEWQGKSLKEQAAALGYQWVENLDALNAVSVANQQKPLLGLFAAGNMPVRWQGPKAT
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCCE
YHGNIDQPAVTCENNPARTADIPTLAAMTEKAIDLLKTNQNGFFLQIEGASIDKQDHAAN
ECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHEECCCCEEEEEECCCCCCHHCCCC
PCGQFGETVDLDEAVQKALAFARADGNTLVIVTADHAHSSQIIAAEAKAPGLTQTLTTKD
CHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCCCCCCEEEEECCC
GAPMTISYGNSEEDSQGHTGTQLRVAAYGPHAANVVGLTDQTDLFYTMRDAMAIK
CCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCEEEEHHHHCCC
>Mature Secondary Structure
MLQPVSLIAGAVLSALLCSAAFADETPVNTASLYERAARGNIVEPGGARRLSGDQTAALK
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
ASLSDKTVKNVILLIGDGMGDSEITSARNYAEGAGGYFKGIDALPLTGQYTHYSLDKKTH
CCCCHHCEEEEEEEEECCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEECCCCCC
KPDYVTDSAASATAWSTGVKTYNGALGVDVNGKDQPTLLEIAKAAGKATGNVSTAELQDA
CCCCCCCCCCCCCHHHCCCCEECCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHCCC
TPAAQISHVTSRKCYGPEETSEKCATNALENGGRGSISEQLLKARADVTLGGGAKSFNQL
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCHHHHHHH
AKSGEWQGKSLKEQAAALGYQWVENLDALNAVSVANQQKPLLGLFAAGNMPVRWQGPKAT
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCCE
YHGNIDQPAVTCENNPARTADIPTLAAMTEKAIDLLKTNQNGFFLQIEGASIDKQDHAAN
ECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHEECCCCEEEEEECCCCCCHHCCCC
PCGQFGETVDLDEAVQKALAFARADGNTLVIVTADHAHSSQIIAAEAKAPGLTQTLTTKD
CHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCCCCCCEEEEECCC
GAPMTISYGNSEEDSQGHTGTQLRVAAYGPHAANVVGLTDQTDLFYTMRDAMAIK
CCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]