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The map label for this gene is setA [H]

Identifier: 157369476

GI number: 157369476

Start: 1357062

End: 1358207

Strand: Direct

Name: setA [H]

Synonym: Spro_1233

Alternate gene names: 157369476

Gene position: 1357062-1358207 (Clockwise)

Preceding gene: 157369475

Following gene: 157369477

Centisome position: 24.91

GC content: 52.62

Gene sequence:

>1146_bases
ATGAATTCGATACGGCTGACCTTTATGGTCGTTGCTTTTTTTTCCGGCATTGCCGGGGCGCTGCAAATACCGACCCTGAG
CCTGTTCCTCACCACCGAGGTGCGGGTGACGTCATTATGGGTCGGTTTTTTTTATGCAGTAAACGCCGGGGTCGGCATTA
TTGTCAGTTTTTTGCTGGCGAAAAAATCAGATCGCCAGCGCGACCGGCGCCATTTGATTTTAATCTGTTATTTATTGGCG
GCGGGAAACTGTCTGTTATTTGCTTTTAATCGTAATTACCTGACGTTAATTACCGCGGGCGTGCTGCTAACCGCCATTGC
CAATACGGCGATACCGCAATTATTTGCCCTGGCGCGTGAATACGGGGAGCGGTCATCGCAAAACGTCGCTATGTTCAGCG
CTATCATGCGCGCCCAACTTTCGCTGGCCTGGGTATTTGGCCCGCCATTGGCTTTTATGCTGGTGGTGAATTATGGCTTC
ACGGCCATGTTTATGTTTGCTGCCGGCATATTCCTGCTGGTCACGCTGCTGGCCTGGTGGATATTACCCGAGGTCACCCG
CCCGCCGGTGGGTAATGAAATCGCCATACCGCAAAAAACGGTCTTCGATAAAGACGTTAGCCGGTTGTTTTTCGCCTCGG
TATTGATGTGGACCTGCAGCGCCATGTATCTGATTGATGTGCCGTTGTATATCACTGCCGAGTTGGGTTTGCCGCAAAGT
CTGACCGGCTGGATGATGGGCGTCGCCGCCGCGATCGAAATCCCGATCATGATCCTGGTAGGGCGCTACGTAAACCGGGT
GGGCAAGCGCCTGATGATGGCGGGGGCGATGGTGGCCGGCATACTGTTTTATGCCGGGATGCTGTGGTTTACCTCGGCCG
CCGGGTTGCTGGTCTGGCAGCTGTTCAACGCGATATTTATCGGCATTATTGCCACCGTCGGCATGCTGTATTTCCAGGAT
TTAATGCCGGAACGGGCGGGCATGGCCACCACTTTATTCACCAACAGTATTTCTACCGGTGTGATCCTGGCCGGAATATT
ACAAGGGATCCTGACCGAATACGGGCCGCATTCCTCCGTTTATCTGTTAGCCATATTACTGCTTTGTATTGCTTTACCTG
TCGGTTTTAAAACCCGGGAAGTTTAG

Upstream 100 bases:

>100_bases
TATAGCCGCGCCGAGAAAGTGTAAATCATAAACCACGGCCCGTCTGTTCGCAGGTGGGCCGTTATTGATCGTTAACAGCA
GCGACCGGCGGTAATCAGCG

Downstream 100 bases:

>100_bases
GTGGTTTCTGAGTGAATCGTTTATTTTTAAGCATAAACAAGGAAATATGATGGCTATATTGGTGACGGGCGGCGCCGGTT
ATATCGGCTCGCATACGGTG

Product: major facilitator transporter

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MNSIRLTFMVVAFFSGIAGALQIPTLSLFLTTEVRVTSLWVGFFYAVNAGVGIIVSFLLAKKSDRQRDRRHLILICYLLA
AGNCLLFAFNRNYLTLITAGVLLTAIANTAIPQLFALAREYGERSSQNVAMFSAIMRAQLSLAWVFGPPLAFMLVVNYGF
TAMFMFAAGIFLLVTLLAWWILPEVTRPPVGNEIAIPQKTVFDKDVSRLFFASVLMWTCSAMYLIDVPLYITAELGLPQS
LTGWMMGVAAAIEIPIMILVGRYVNRVGKRLMMAGAMVAGILFYAGMLWFTSAAGLLVWQLFNAIFIGIIATVGMLYFQD
LMPERAGMATTLFTNSISTGVILAGILQGILTEYGPHSSVYLLAILLLCIALPVGFKTREV

Sequences:

>Translated_381_residues
MNSIRLTFMVVAFFSGIAGALQIPTLSLFLTTEVRVTSLWVGFFYAVNAGVGIIVSFLLAKKSDRQRDRRHLILICYLLA
AGNCLLFAFNRNYLTLITAGVLLTAIANTAIPQLFALAREYGERSSQNVAMFSAIMRAQLSLAWVFGPPLAFMLVVNYGF
TAMFMFAAGIFLLVTLLAWWILPEVTRPPVGNEIAIPQKTVFDKDVSRLFFASVLMWTCSAMYLIDVPLYITAELGLPQS
LTGWMMGVAAAIEIPIMILVGRYVNRVGKRLMMAGAMVAGILFYAGMLWFTSAAGLLVWQLFNAIFIGIIATVGMLYFQD
LMPERAGMATTLFTNSISTGVILAGILQGILTEYGPHSSVYLLAILLLCIALPVGFKTREV
>Mature_381_residues
MNSIRLTFMVVAFFSGIAGALQIPTLSLFLTTEVRVTSLWVGFFYAVNAGVGIIVSFLLAKKSDRQRDRRHLILICYLLA
AGNCLLFAFNRNYLTLITAGVLLTAIANTAIPQLFALAREYGERSSQNVAMFSAIMRAQLSLAWVFGPPLAFMLVVNYGF
TAMFMFAAGIFLLVTLLAWWILPEVTRPPVGNEIAIPQKTVFDKDVSRLFFASVLMWTCSAMYLIDVPLYITAELGLPQS
LTGWMMGVAAAIEIPIMILVGRYVNRVGKRLMMAGAMVAGILFYAGMLWFTSAAGLLVWQLFNAIFIGIIATVGMLYFQD
LMPERAGMATTLFTNSISTGVILAGILQGILTEYGPHSSVYLLAILLLCIALPVGFKTREV

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport IPTG, lactose and glucose. Has broad substrate specificity, with preferences for glucosides or galactosides with alkyl or

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI48994875, Length=382, Percent_Identity=58.9005235602094, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1790091, Length=367, Percent_Identity=47.4114441416894, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1788495, Length=376, Percent_Identity=46.0106382978723, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41817; Mature: 41817

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIRLTFMVVAFFSGIAGALQIPTLSLFLTTEVRVTSLWVGFFYAVNAGVGIIVSFLLA
CCCHHHHHHHHHHHHHHCCHHCCCEEEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KKSDRQRDRRHLILICYLLAAGNCLLFAFNRNYLTLITAGVLLTAIANTAIPQLFALARE
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGERSSQNVAMFSAIMRAQLSLAWVFGPPLAFMLVVNYGFTAMFMFAAGIFLLVTLLAWW
HCCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILPEVTRPPVGNEIAIPQKTVFDKDVSRLFFASVLMWTCSAMYLIDVPLYITAELGLPQS
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCH
LTGWMMGVAAAIEIPIMILVGRYVNRVGKRLMMAGAMVAGILFYAGMLWFTSAAGLLVWQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFNAIFIGIIATVGMLYFQDLMPERAGMATTLFTNSISTGVILAGILQGILTEYGPHSSV
HHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
YLLAILLLCIALPVGFKTREV
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNSIRLTFMVVAFFSGIAGALQIPTLSLFLTTEVRVTSLWVGFFYAVNAGVGIIVSFLLA
CCCHHHHHHHHHHHHHHCCHHCCCEEEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KKSDRQRDRRHLILICYLLAAGNCLLFAFNRNYLTLITAGVLLTAIANTAIPQLFALARE
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGERSSQNVAMFSAIMRAQLSLAWVFGPPLAFMLVVNYGFTAMFMFAAGIFLLVTLLAWW
HCCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILPEVTRPPVGNEIAIPQKTVFDKDVSRLFFASVLMWTCSAMYLIDVPLYITAELGLPQS
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCH
LTGWMMGVAAAIEIPIMILVGRYVNRVGKRLMMAGAMVAGILFYAGMLWFTSAAGLLVWQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFNAIFIGIIATVGMLYFQDLMPERAGMATTLFTNSISTGVILAGILQGILTEYGPHSSV
HHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
YLLAILLLCIALPVGFKTREV
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 10209755; 10438463 [H]