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The map label for this gene is yneF [H]

Identifier: 157369424

GI number: 157369424

Start: 1298717

End: 1300165

Strand: Direct

Name: yneF [H]

Synonym: Spro_1181

Alternate gene names: 157369424

Gene position: 1298717-1300165 (Clockwise)

Preceding gene: 157369423

Following gene: 157369426

Centisome position: 23.83

GC content: 55.07

Gene sequence:

>1449_bases
ATGAACGCCAGAATAAGCGACAAACATGGCTTGCCTGTGGCTATCCAGCTGATTACGCTTTTTCTGCTGGCATTTTTGCT
TTCCCTGCTGGGTATTTTTACCCGCCCAATAGGATCGCTTTCTCTGTTTTGGCCGGTGAATGCGATTTTACTCGGTCTGC
TATTACGCAAACCCGCCTATGCGAGTCCGGCCGGTTGGCTGGCTATCTATATTGGGATGATCGCCGCAGATTTGGCCAAC
GGTGAGAGTCTGTCGCTGGCGATGTGGTTGAACGCCTGCAATATGTCGCTGATTGCTACCGGTTACGGCGTGATGTCGCT
GTTGCCAAAGTCGCAACGCCGGATGGGTAAACCCCAGACTACCCTTTATATGTTTACCGCCAGCCTGAGTAGCGCGGCGG
TGGCGTCGACCTTATCGATACTGCGTAATGACAGCCTGTACAACAACACGGCCGTTATCGCCTGGTTAGCCTGGTTCAGC
GAGCAGTTCTCGACCAATCTGCTGTTGTTGCCGGTGCTGCTGGCGGCACCGCGTCTCAAACAGCTGTTGCGTATGCAGAT
CAACTGGCAACTGCGTGCCAGTATGCCGCTGCTGGCGTTGCTGTTTTCACTGGTGTTCAGTGTGTATATCGGCGGTCCAG
GGGCGATTGCCTTCCCCATTCCGGCGCTGCTGTGGTGCGCGGTGCGCTACCAACTGTTCACGGTAACCTTGTTGACGCTG
CTGACTGGGATGACCGAGATCAGCAGTATCTCCGCCAATCTGATGCTGTATGAAACGCCCAACAACCACATTGCCTTCCT
CGACACGCTGATGTCAGCCCGTTTGGGGATCGCCATGCTGGTGATGGGTCCACTGATCCTTGCCAGTTCAATTGCCGTTA
ACCGTAAGCTGCTGCGGCGGCTGGAGCACAGCGCCAATCACGATTTTCTGACCGGCGTACTGGCGCGTAATGCACTAACG
CGCAAGGCTGGCGAACTGCTGGAGCACAAGCATAAATCGAAGGAGGCGGTGTCGCTGCTGCTGATCGATATCGATCATTT
TAAGCAGATCAATGATACCCATGGGCATCTGGTGGGCGATCAGGTACTGGCTTCATTCGCCCATATTGTGCGGCGCGAGT
TGCGTCACGATCAGCTGTTTGGCCGTTTGGGTGGGGAAGAGTTTGCCATTATGCTGCCGCGGGCGCTGGCGGCGCAGGGG
GTGGCACTGGCAGAGCATTTGCGGCAATTGGTACAAAAAGCCGATTTGCTCCCCACTGGCAAGGTGCCGTTGAACATCAC
CATCAGCGTTGGCGTCGCCAGTCTGGGGATGAACGAAGTTAAATCGCTGGAACAGTTAATGAATATGGCGGATATCGCGC
TTTATCGCGCCAAGTCGCAGGGGCGTAACCGGGTGGAGTCGTTTAACGCGGTAAGTGGCAACAGCGTTGGCCACATCATG
TTTAAATAA

Upstream 100 bases:

>100_bases
TTTTTTAAGTCTGCCTCTGGTTTTTATGTCATTTTCATTATGCCGGGCGAGGGAATATGCATAGAATTGCTCTGGTTATT
ATAAATATATGGGTAAGGCG

Downstream 100 bases:

>100_bases
GGGATTCAGTTCTGCTTGGGTTCAAACTCGGTACCGCAGTTTGGGCAGATAAGCATGACATTTTGCCGCAGCATCGAGCA
TTTTTGAGTGGTGACGTAGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNARISDKHGLPVAIQLITLFLLAFLLSLLGIFTRPIGSLSLFWPVNAILLGLLLRKPAYASPAGWLAIYIGMIAADLAN
GESLSLAMWLNACNMSLIATGYGVMSLLPKSQRRMGKPQTTLYMFTASLSSAAVASTLSILRNDSLYNNTAVIAWLAWFS
EQFSTNLLLLPVLLAAPRLKQLLRMQINWQLRASMPLLALLFSLVFSVYIGGPGAIAFPIPALLWCAVRYQLFTVTLLTL
LTGMTEISSISANLMLYETPNNHIAFLDTLMSARLGIAMLVMGPLILASSIAVNRKLLRRLEHSANHDFLTGVLARNALT
RKAGELLEHKHKSKEAVSLLLIDIDHFKQINDTHGHLVGDQVLASFAHIVRRELRHDQLFGRLGGEEFAIMLPRALAAQG
VALAEHLRQLVQKADLLPTGKVPLNITISVGVASLGMNEVKSLEQLMNMADIALYRAKSQGRNRVESFNAVSGNSVGHIM
FK

Sequences:

>Translated_482_residues
MNARISDKHGLPVAIQLITLFLLAFLLSLLGIFTRPIGSLSLFWPVNAILLGLLLRKPAYASPAGWLAIYIGMIAADLAN
GESLSLAMWLNACNMSLIATGYGVMSLLPKSQRRMGKPQTTLYMFTASLSSAAVASTLSILRNDSLYNNTAVIAWLAWFS
EQFSTNLLLLPVLLAAPRLKQLLRMQINWQLRASMPLLALLFSLVFSVYIGGPGAIAFPIPALLWCAVRYQLFTVTLLTL
LTGMTEISSISANLMLYETPNNHIAFLDTLMSARLGIAMLVMGPLILASSIAVNRKLLRRLEHSANHDFLTGVLARNALT
RKAGELLEHKHKSKEAVSLLLIDIDHFKQINDTHGHLVGDQVLASFAHIVRRELRHDQLFGRLGGEEFAIMLPRALAAQG
VALAEHLRQLVQKADLLPTGKVPLNITISVGVASLGMNEVKSLEQLMNMADIALYRAKSQGRNRVESFNAVSGNSVGHIM
FK
>Mature_482_residues
MNARISDKHGLPVAIQLITLFLLAFLLSLLGIFTRPIGSLSLFWPVNAILLGLLLRKPAYASPAGWLAIYIGMIAADLAN
GESLSLAMWLNACNMSLIATGYGVMSLLPKSQRRMGKPQTTLYMFTASLSSAAVASTLSILRNDSLYNNTAVIAWLAWFS
EQFSTNLLLLPVLLAAPRLKQLLRMQINWQLRASMPLLALLFSLVFSVYIGGPGAIAFPIPALLWCAVRYQLFTVTLLTL
LTGMTEISSISANLMLYETPNNHIAFLDTLMSARLGIAMLVMGPLILASSIAVNRKLLRRLEHSANHDFLTGVLARNALT
RKAGELLEHKHKSKEAVSLLLIDIDHFKQINDTHGHLVGDQVLASFAHIVRRELRHDQLFGRLGGEEFAIMLPRALAAQG
VALAEHLRQLVQKADLLPTGKVPLNITISVGVASLGMNEVKSLEQLMNMADIALYRAKSQGRNRVESFNAVSGNSVGHIM
FK

Specific function: Unknown

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=315, Percent_Identity=37.1428571428571, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI87081881, Length=186, Percent_Identity=39.7849462365591, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=35.5029585798817, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=38.1818181818182, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1786584, Length=210, Percent_Identity=31.4285714285714, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788381, Length=188, Percent_Identity=30.8510638297872, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693134, Length=168, Percent_Identity=35.1190476190476, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=34.9397590361446, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787541, Length=189, Percent_Identity=31.7460317460317, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=34.3558282208589, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787056, Length=175, Percent_Identity=31.4285714285714, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=184, Percent_Identity=27.7173913043478, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=172, Percent_Identity=36.6279069767442, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 52790; Mature: 52790

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARISDKHGLPVAIQLITLFLLAFLLSLLGIFTRPIGSLSLFWPVNAILLGLLLRKPAY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCC
ASPAGWLAIYIGMIAADLANGESLSLAMWLNACNMSLIATGYGVMSLLPKSQRRMGKPQT
CCCHHHHHHHHHHHHHHHCCCCCEEHEEEHHHCCCHHHHHCHHHHHHCCHHHHHCCCCCC
TLYMFTASLSSAAVASTLSILRNDSLYNNTAVIAWLAWFSEQFSTNLLLLPVLLAAPRLK
EEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
QLLRMQINWQLRASMPLLALLFSLVFSVYIGGPGAIAFPIPALLWCAVRYQLFTVTLLTL
HHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTGMTEISSISANLMLYETPNNHIAFLDTLMSARLGIAMLVMGPLILASSIAVNRKLLRR
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEHSANHDFLTGVLARNALTRKAGELLEHKHKSKEAVSLLLIDIDHFKQINDTHGHLVGD
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEHHHHHHCCCCCCCCHHH
QVLASFAHIVRRELRHDQLFGRLGGEEFAIMLPRALAAQGVALAEHLRQLVQKADLLPTG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
KVPLNITISVGVASLGMNEVKSLEQLMNMADIALYRAKSQGRNRVESFNAVSGNSVGHIM
CCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCEEEE
FK
EC
>Mature Secondary Structure
MNARISDKHGLPVAIQLITLFLLAFLLSLLGIFTRPIGSLSLFWPVNAILLGLLLRKPAY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCC
ASPAGWLAIYIGMIAADLANGESLSLAMWLNACNMSLIATGYGVMSLLPKSQRRMGKPQT
CCCHHHHHHHHHHHHHHHCCCCCEEHEEEHHHCCCHHHHHCHHHHHHCCHHHHHCCCCCC
TLYMFTASLSSAAVASTLSILRNDSLYNNTAVIAWLAWFSEQFSTNLLLLPVLLAAPRLK
EEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
QLLRMQINWQLRASMPLLALLFSLVFSVYIGGPGAIAFPIPALLWCAVRYQLFTVTLLTL
HHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTGMTEISSISANLMLYETPNNHIAFLDTLMSARLGIAMLVMGPLILASSIAVNRKLLRR
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEHSANHDFLTGVLARNALTRKAGELLEHKHKSKEAVSLLLIDIDHFKQINDTHGHLVGD
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEHHHHHHCCCCCCCCHHH
QVLASFAHIVRRELRHDQLFGRLGGEEFAIMLPRALAAQGVALAEHLRQLVQKADLLPTG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
KVPLNITISVGVASLGMNEVKSLEQLMNMADIALYRAKSQGRNRVESFNAVSGNSVGHIM
CCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCEEEE
FK
EC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]