Click here to switch to the map view.

The map label for this gene is nemA [H]

Identifier: 157369415

GI number: 157369415

Start: 1289330

End: 1290409

Strand: Direct

Name: nemA [H]

Synonym: Spro_1172

Alternate gene names: 157369415

Gene position: 1289330-1290409 (Clockwise)

Preceding gene: 157369414

Following gene: 157369416

Centisome position: 23.66

GC content: 44.07

Gene sequence:

>1080_bases
ATGACAAATAAAAAACTATTTAGTCCATATAAGCTTGGTCAGATCACCCTTTCCAACAGGATTGTCCTTGCACCTCTTAC
CCGCAGTCGGGCTGGTAAAGGTTTTGTCCCTGGCGAATTTGCGGCAACTTATTATAGTCAGCGTGCATCTGCCGGCCTTT
TAATAGCGGAAGCCACTCAGATTTCTCAACAGGGGCAAGGGTATCAAGATACGCCAGGAATATATACACAGGATCAAATT
GAGGGTTGGCGAAAAGTCACTGATGCGGTACATGCCAAGGGAGGTGTTATTTTTCTGCAGATTTGGCATGTTGGCCGAAT
TTCACATGTAGAGCTTCAAAAAAATGGTGCGGCGCCGGTAGCACCTTCTGAAATTCAGGCAAATGCGAAGGTGTTTATAA
ATAACAGTTTAGTGCAAACTTCAATTCCGCGAGCTCTGAGTATTGATGAAATACCCGGTATCGTGGATGATTTCCGGCAA
GCCGCTGCAAATGCGATCGCTGCTGGATTTGACGGTGTTGAGGTTCACGGTGCTAATGGTTATTTACTTGATCAATTTGT
TCGCGATAATGCCAATAACCGTGTTGATGCTTATGGTGGTTCAATTGAAAACCGTGCCCGTTTGTTGCTAGAGGTGACGA
AGGCCGTGATTGAAGAGGTTGGTGCTGATCGTACTGGGGTTCGTATTTCTCCCGTCTCGCCTATTAATGATGTTGTTAGC
CGTGAGCCTCAGGCTCAGTTTAATTATATCGCTACTGAACTCAGTAAGTTAGGCGCCGTTTATCTTCATGTTGTAGAAGG
TGCAACTGGCGGGGCACGTGATGATGTACCGTTTGATTTTGACACTCTGCGTCAAAACTTCAAAAACACCTACATTGCAA
ATAATGGGTATGATCTGAATTTGGCAAATATATCTCTTGCAGAGAATAAGTCTGATTTGATTTCCTTTGGTCGGTTTTTC
ATCAGTAACCCAGATTTAGTAGAAAGATTTAAAAGCGGTTCCTCTTTATCTGAAATTGACGCTGAACACCTCTATGGTGG
GGGGGCAAAAGGCTATACCGACTATCCAACCTTGAGTTAA

Upstream 100 bases:

>100_bases
TATTTTTTTTTGCATTACTCTAAGTTCACATTACATCTGTTAAATTTAGGCGTTATTTTTATTTTATATAGTCGGAAATA
TAAATTTATAGAGGTCAGTC

Downstream 100 bases:

>100_bases
GACCAGCAGCGGTATTGACGTAGCCCGACTGTTTTTTATTCAAACGGTTGGGCTTTTTTGCTTAATCGTACATCTTACAT
ATGGCATGCTTCTGATATTT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTNKKLFSPYKLGQITLSNRIVLAPLTRSRAGKGFVPGEFAATYYSQRASAGLLIAEATQISQQGQGYQDTPGIYTQDQI
EGWRKVTDAVHAKGGVIFLQIWHVGRISHVELQKNGAAPVAPSEIQANAKVFINNSLVQTSIPRALSIDEIPGIVDDFRQ
AAANAIAAGFDGVEVHGANGYLLDQFVRDNANNRVDAYGGSIENRARLLLEVTKAVIEEVGADRTGVRISPVSPINDVVS
REPQAQFNYIATELSKLGAVYLHVVEGATGGARDDVPFDFDTLRQNFKNTYIANNGYDLNLANISLAENKSDLISFGRFF
ISNPDLVERFKSGSSLSEIDAEHLYGGGAKGYTDYPTLS

Sequences:

>Translated_359_residues
MTNKKLFSPYKLGQITLSNRIVLAPLTRSRAGKGFVPGEFAATYYSQRASAGLLIAEATQISQQGQGYQDTPGIYTQDQI
EGWRKVTDAVHAKGGVIFLQIWHVGRISHVELQKNGAAPVAPSEIQANAKVFINNSLVQTSIPRALSIDEIPGIVDDFRQ
AAANAIAAGFDGVEVHGANGYLLDQFVRDNANNRVDAYGGSIENRARLLLEVTKAVIEEVGADRTGVRISPVSPINDVVS
REPQAQFNYIATELSKLGAVYLHVVEGATGGARDDVPFDFDTLRQNFKNTYIANNGYDLNLANISLAENKSDLISFGRFF
ISNPDLVERFKSGSSLSEIDAEHLYGGGAKGYTDYPTLS
>Mature_358_residues
TNKKLFSPYKLGQITLSNRIVLAPLTRSRAGKGFVPGEFAATYYSQRASAGLLIAEATQISQQGQGYQDTPGIYTQDQIE
GWRKVTDAVHAKGGVIFLQIWHVGRISHVELQKNGAAPVAPSEIQANAKVFINNSLVQTSIPRALSIDEIPGIVDDFRQA
AANAIAAGFDGVEVHGANGYLLDQFVRDNANNRVDAYGGSIENRARLLLEVTKAVIEEVGADRTGVRISPVSPINDVVSR
EPQAQFNYIATELSKLGAVYLHVVEGATGGARDDVPFDFDTLRQNFKNTYIANNGYDLNLANISLAENKSDLISFGRFFI
SNPDLVERFKSGSSLSEIDAEHLYGGGAKGYTDYPTLS

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=47.945205479452, Blast_Score=337, Evalue=6e-94,
Organism=Escherichia coli, GI1789463, Length=349, Percent_Identity=30.0859598853868, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17559804, Length=141, Percent_Identity=36.8794326241135, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17565138, Length=268, Percent_Identity=26.4925373134328, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=275, Percent_Identity=28.7272727272727, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=276, Percent_Identity=27.8985507246377, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=295, Percent_Identity=27.7966101694915, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=269, Percent_Identity=27.8810408921933, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=253, Percent_Identity=29.2490118577075, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=388, Percent_Identity=36.340206185567, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6321973, Length=389, Percent_Identity=35.4755784061697, Blast_Score=210, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38930; Mature: 38799

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKKLFSPYKLGQITLSNRIVLAPLTRSRAGKGFVPGEFAATYYSQRASAGLLIAEATQ
CCCCCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHH
ISQQGQGYQDTPGIYTQDQIEGWRKVTDAVHAKGGVIFLQIWHVGRISHVELQKNGAAPV
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCC
APSEIQANAKVFINNSLVQTSIPRALSIDEIPGIVDDFRQAAANAIAAGFDGVEVHGANG
CCHHHCCCCEEEEECCCHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCC
YLLDQFVRDNANNRVDAYGGSIENRARLLLEVTKAVIEEVGADRTGVRISPVSPINDVVS
HHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHC
REPQAQFNYIATELSKLGAVYLHVVEGATGGARDDVPFDFDTLRQNFKNTYIANNGYDLN
CCCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEE
LANISLAENKSDLISFGRFFISNPDLVERFKSGSSLSEIDAEHLYGGGAKGYTDYPTLS
EEEEEECCCHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TNKKLFSPYKLGQITLSNRIVLAPLTRSRAGKGFVPGEFAATYYSQRASAGLLIAEATQ
CCCCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHH
ISQQGQGYQDTPGIYTQDQIEGWRKVTDAVHAKGGVIFLQIWHVGRISHVELQKNGAAPV
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCC
APSEIQANAKVFINNSLVQTSIPRALSIDEIPGIVDDFRQAAANAIAAGFDGVEVHGANG
CCHHHCCCCEEEEECCCHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCC
YLLDQFVRDNANNRVDAYGGSIENRARLLLEVTKAVIEEVGADRTGVRISPVSPINDVVS
HHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHC
REPQAQFNYIATELSKLGAVYLHVVEGATGGARDDVPFDFDTLRQNFKNTYIANNGYDLN
CCCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEE
LANISLAENKSDLISFGRFFISNPDLVERFKSGSSLSEIDAEHLYGGGAKGYTDYPTLS
EEEEEECCCHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]