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The map label for this gene is amtB [H]

Identifier: 157369353

GI number: 157369353

Start: 1222338

End: 1223624

Strand: Direct

Name: amtB [H]

Synonym: Spro_1110

Alternate gene names: 157369353

Gene position: 1222338-1223624 (Clockwise)

Preceding gene: 157369352

Following gene: 157369355

Centisome position: 22.43

GC content: 59.13

Gene sequence:

>1287_bases
ATGAAAAAACTTTTATCCATGTTGGGCCTGAGTACGGTAACCATGGTTCCTTCTTTGGCACTGGCCGCTCCGGCGGTCGC
AGACAAGGCGGATAACGCCTTCATGATGATTTGCACCGCTTTGGTGCTGTTCATGACCCTGCCGGGTGTCGCTCTGTTTT
ACGGCGGTTTGCTGCGTTCCAAAAACGTGCTCTCCATGTTGACCCAGGTCACCGTTACCTTTGCCTTGGTGTGCGTGCTG
TGGGTGCTTTATGGCTACAGCCTGGCTTTCAGCGAAGGTAACGCCTTCTTCGGCGGTTTCCAGACGGCGATGCTCAAAGG
CATTGGCATCGACAGCGTCACCGGGACCTTTAGCCAGATGATCCACGTGGCGTTCCAGTGTTCGTTCGCCTGTATTACCG
TTGCGCTGGTGGTGGGGGGCTTTGCCGAGCGTATCCGCTTCTCTGCGGTGGTGATCTTCGCAGCCATCTGGTTCACCATT
TCTTACCTGCCGATTGCCCACATGGTGTGGGGCGGCGGCTTCCTGGCCGCTGATGGCGCGTTGGACTTTGCCGGCGGTAC
CGTTGTGCACATCAACGCAGCGAGTGCCGGTCTGGTTGGCGCTTACCTGCTGGGCAAGCGTGCCGGTTTCGGTAAGGAAG
CTTTCAAACCGCACAACCTGCCCATGGTGTTTACCGGGGCTTCCATCCTGTATATCGGCTGGTTCGGCTTTAACGCCGGT
TCTGCCAGCGCGGCTAACGGCATTGCGGCCCTGGCGTTCCTGAATACCGTGGTGGCGACTGCCGGCGCGATCCTCTCCTG
GACCTTTGCCGAGTGGATTGTTCGTGGCAAGCCTTCCCTGTTGGGCGCCTGTTCCGGCATGATCGCCGGGCTGGTTGCCA
TCACGCCGGCTGCAGGCACCGTGGGTATTGGCGGGGCGCTGATTATCGGCCTGATTGGCGGTATCGCCGGTCTGTGGGGC
GTGGTCACGCTGAAGAAATGGCTGAAGGTAGATGATACCTGCGACGTGTTCGGTGTGCATGGCGTGTGCGGTATCGTCGG
TTGCCTGCTGACTGGGGTATTCACCTCGGCCTCGTTGGGCGGCACTGGGTACGCCGAGGGCGTCACCATGGGCCATCAGG
TGTGGATCCAGTTGTTGAGCGTAGTGATTACGCTGGTGTGGTCTGGTGTTGCTGCCTTTATCGCCTTTAAAGTGGCAGGG
GCCATCGTCGGGCTGCGCGTACCGGAAGAGCAGGAACGCGAAGGTCTGGACGTCAACAGCCATGGCGAGAGTGCCTATAA
CCAATAA

Upstream 100 bases:

>100_bases
CGTTGCCGAATTGCAACGTGTCATTCGCATTCGCACCGGCGAAACAGACGAAGCAGCACTGTAATCACTGGCTCAGTAAA
GTAATGGGGATGGATTGATA

Downstream 100 bases:

>100_bases
GCCTTGAAGAAGGGCAGTGCGCTAAAATAACTCGAAAAATAATGATGATGCAAAAAGGGCGATGTCACCATCGCCCTTTT
TTTTGGCCTGCGTTTATCAG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKKLLSMLGLSTVTMVPSLALAAPAVADKADNAFMMICTALVLFMTLPGVALFYGGLLRSKNVLSMLTQVTVTFALVCVL
WVLYGYSLAFSEGNAFFGGFQTAMLKGIGIDSVTGTFSQMIHVAFQCSFACITVALVVGGFAERIRFSAVVIFAAIWFTI
SYLPIAHMVWGGGFLAADGALDFAGGTVVHINAASAGLVGAYLLGKRAGFGKEAFKPHNLPMVFTGASILYIGWFGFNAG
SASAANGIAALAFLNTVVATAGAILSWTFAEWIVRGKPSLLGACSGMIAGLVAITPAAGTVGIGGALIIGLIGGIAGLWG
VVTLKKWLKVDDTCDVFGVHGVCGIVGCLLTGVFTSASLGGTGYAEGVTMGHQVWIQLLSVVITLVWSGVAAFIAFKVAG
AIVGLRVPEEQEREGLDVNSHGESAYNQ

Sequences:

>Translated_428_residues
MKKLLSMLGLSTVTMVPSLALAAPAVADKADNAFMMICTALVLFMTLPGVALFYGGLLRSKNVLSMLTQVTVTFALVCVL
WVLYGYSLAFSEGNAFFGGFQTAMLKGIGIDSVTGTFSQMIHVAFQCSFACITVALVVGGFAERIRFSAVVIFAAIWFTI
SYLPIAHMVWGGGFLAADGALDFAGGTVVHINAASAGLVGAYLLGKRAGFGKEAFKPHNLPMVFTGASILYIGWFGFNAG
SASAANGIAALAFLNTVVATAGAILSWTFAEWIVRGKPSLLGACSGMIAGLVAITPAAGTVGIGGALIIGLIGGIAGLWG
VVTLKKWLKVDDTCDVFGVHGVCGIVGCLLTGVFTSASLGGTGYAEGVTMGHQVWIQLLSVVITLVWSGVAAFIAFKVAG
AIVGLRVPEEQEREGLDVNSHGESAYNQ
>Mature_428_residues
MKKLLSMLGLSTVTMVPSLALAAPAVADKADNAFMMICTALVLFMTLPGVALFYGGLLRSKNVLSMLTQVTVTFALVCVL
WVLYGYSLAFSEGNAFFGGFQTAMLKGIGIDSVTGTFSQMIHVAFQCSFACITVALVVGGFAERIRFSAVVIFAAIWFTI
SYLPIAHMVWGGGFLAADGALDFAGGTVVHINAASAGLVGAYLLGKRAGFGKEAFKPHNLPMVFTGASILYIGWFGFNAG
SASAANGIAALAFLNTVVATAGAILSWTFAEWIVRGKPSLLGACSGMIAGLVAITPAAGTVGIGGALIIGLIGGIAGLWG
VVTLKKWLKVDDTCDVFGVHGVCGIVGCLLTGVFTSASLGGTGYAEGVTMGHQVWIQLLSVVITLVWSGVAAFIAFKVAG
AIVGLRVPEEQEREGLDVNSHGESAYNQ

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=427, Percent_Identity=74.9414519906323, Blast_Score=653, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550182, Length=436, Percent_Identity=28.8990825688073, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17550180, Length=434, Percent_Identity=27.8801843317972, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32564042, Length=418, Percent_Identity=24.6411483253589, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI133931100, Length=446, Percent_Identity=25.1121076233184, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6325396, Length=413, Percent_Identity=35.1089588377724, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=409, Percent_Identity=33.9853300733496, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6324187, Length=421, Percent_Identity=35.8669833729216, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI161078314, Length=419, Percent_Identity=27.2076372315036, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44209; Mature: 44209

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLSMLGLSTVTMVPSLALAAPAVADKADNAFMMICTALVLFMTLPGVALFYGGLLRS
CHHHHHHHCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHC
KNVLSMLTQVTVTFALVCVLWVLYGYSLAFSEGNAFFGGFQTAMLKGIGIDSVTGTFSQM
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
IHVAFQCSFACITVALVVGGFAERIRFSAVVIFAAIWFTISYLPIAHMVWGGGFLAADGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
LDFAGGTVVHINAASAGLVGAYLLGKRAGFGKEAFKPHNLPMVFTGASILYIGWFGFNAG
EECCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCEEEEEEEECCCCC
SASAANGIAALAFLNTVVATAGAILSWTFAEWIVRGKPSLLGACSGMIAGLVAITPAAGT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
VGIGGALIIGLIGGIAGLWGVVTLKKWLKVDDTCDVFGVHGVCGIVGCLLTGVFTSASLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
GTGYAEGVTMGHQVWIQLLSVVITLVWSGVAAFIAFKVAGAIVGLRVPEEQEREGLDVNS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
HGESAYNQ
CCCCCCCC
>Mature Secondary Structure
MKKLLSMLGLSTVTMVPSLALAAPAVADKADNAFMMICTALVLFMTLPGVALFYGGLLRS
CHHHHHHHCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHC
KNVLSMLTQVTVTFALVCVLWVLYGYSLAFSEGNAFFGGFQTAMLKGIGIDSVTGTFSQM
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
IHVAFQCSFACITVALVVGGFAERIRFSAVVIFAAIWFTISYLPIAHMVWGGGFLAADGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
LDFAGGTVVHINAASAGLVGAYLLGKRAGFGKEAFKPHNLPMVFTGASILYIGWFGFNAG
EECCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCEEEEEEEECCCCC
SASAANGIAALAFLNTVVATAGAILSWTFAEWIVRGKPSLLGACSGMIAGLVAITPAAGT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
VGIGGALIIGLIGGIAGLWGVVTLKKWLKVDDTCDVFGVHGVCGIVGCLLTGVFTSASLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
GTGYAEGVTMGHQVWIQLLSVVITLVWSGVAAFIAFKVAGAIVGLRVPEEQEREGLDVNS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
HGESAYNQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]