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The map label for this gene is thiI

Identifier: 157369324

GI number: 157369324

Start: 1189053

End: 1190501

Strand: Direct

Name: thiI

Synonym: Spro_1081

Alternate gene names: 157369324

Gene position: 1189053-1190501 (Clockwise)

Preceding gene: 157369319

Following gene: 157369327

Centisome position: 21.82

GC content: 55.21

Gene sequence:

>1449_bases
ATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAGAGCCAATCTGTGCGCTTGCGCTTTATCAAGATCCTCTC
GACCAGTATTCGCAACGTCCTGAAGCAGTATGATGAAACACTGGCGGTTGTCCGTCACTGGGATCATATCGAAGTTCGCG
CCAAAGATGAAAACCAGCGGCCGGTGATTGCTGACGCGCTGACGCGCATTCCCGGTATCCACCACATTCTGGAAGTGGAA
GACCGCGACTATACCGATATCCACCATATTTTCGAGCAGACGCTGGAAGCCTACCGCGAGCAACTGGAAGGCAAGACCTT
CTGCGTGCGCGTTAAGCGCCGCGGCAAGCAGGACTTCAATTCGCAGGACGTGGAGCGTTACGTCGGTGGCGGTCTGAACC
AGCATATTGAAAGCGCGCGCGTTAAGCTGTCGCATCCGCAGGTGACGGTTAACCTGGAAATCGAAAACGACAAGCTGATG
CTGGTGAAAGCCCGCCGTGAAGGCATCGGCGGCTACCCGGTAGGCACCCAGGAAGACGTGCTGTCGCTGATTTCCGGTGG
TTTCGACTCGGGTGTTTCCAGTTATATGCTGATGCGCCGCGGTTGCCGTGTGCATTATTGCTTCTTCAATCTGGGTGGCG
CGGCGCATGAAATTGGCGTGCGCCAGGTAGCTCACTATCTGTGGAACCGCTTTGCCAGTTCGCACAAGGTGCGCTTTATC
GCCATCGATTTTGAACCTGTGGTTGGCGAGATCCTGGAAAAGGTCGAAGACGGCCAGATGGGCGTGGTGCTCAAGCGCAT
GATGGTGCGTGCCGCTTCGCAGATTGCCGAACGTTATGGCGTGCAGGCATTGGTGACCGGTGAAGCGCTGGGGCAGGTAT
CCAGCCAGACGCTGACTAACCTGCGCCTGATCGACAACGCGTCCGATACGCTGATCCTGCGTCCGCTGATCTCCCACGAC
AAAGAGCACATCATCAAAGTGGCGCGTGAGATCGGCACCGAGGACTTCGCCAAGACCATGCCGGAGTACTGCGGTGTGAT
CTCGAAAAGCCCGACGGTGAAGGCGATTAAAGCCAAGATCGAAGAAGAAGAAGGGCACTTTGATTTCAGCATTCTTGATC
GCGTGGTTAGCGAAGCCAAGAACGTGGATATTCGCACCATCGCCGAGCAGACTCAGGAGCAGGTTACCGAAGTCGAAACC
GTGGCGGAGTTCGATGCTGACCAGGTGATTCTGGATATTCGTTCTAACGACGAGCAGGAAGATAAACCGCTGAAGCTGGA
TCAGGTTGAGGTGAAACCGCTGCCGTTCTACAAGCTCAGCACCCAGTTTGGCGATTTGGATCAGAGCAAAACTTACCTGC
TGTACTGTGAGCGCGGCGTGATGAGCCGCCTGCAGGCGCTGTACCTGCTGGAGCAGGGGTTCACCAACGTGAAGGTTTAC
CGCCCATAA

Upstream 100 bases:

>100_bases
CAAATGCGATTGATAAAACATTCGGCAGCCCCGTTGGTTTTATCAATCGCATATACATTGTAAATCCTTTTCCCTCGTAA
TTCTGACAATAAAGACCGCC

Downstream 100 bases:

>100_bases
TTAATTCAGGGGGCGTATCTGCCGCCCCCATGATTTAATCGCGGAAGTCGTACATTCCAGGTATCAATACCAGCTGAGCG
GCGATCTCCGCCGCTTTGGC

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKFIIKLFPEITIKSQSVRLRFIKILSTSIRNVLKQYDETLAVVRHWDHIEVRAKDENQRPVIADALTRIPGIHHILEVE
DRDYTDIHHIFEQTLEAYREQLEGKTFCVRVKRRGKQDFNSQDVERYVGGGLNQHIESARVKLSHPQVTVNLEIENDKLM
LVKARREGIGGYPVGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFASSHKVRFI
AIDFEPVVGEILEKVEDGQMGVVLKRMMVRAASQIAERYGVQALVTGEALGQVSSQTLTNLRLIDNASDTLILRPLISHD
KEHIIKVAREIGTEDFAKTMPEYCGVISKSPTVKAIKAKIEEEEGHFDFSILDRVVSEAKNVDIRTIAEQTQEQVTEVET
VAEFDADQVILDIRSNDEQEDKPLKLDQVEVKPLPFYKLSTQFGDLDQSKTYLLYCERGVMSRLQALYLLEQGFTNVKVY
RP

Sequences:

>Translated_482_residues
MKFIIKLFPEITIKSQSVRLRFIKILSTSIRNVLKQYDETLAVVRHWDHIEVRAKDENQRPVIADALTRIPGIHHILEVE
DRDYTDIHHIFEQTLEAYREQLEGKTFCVRVKRRGKQDFNSQDVERYVGGGLNQHIESARVKLSHPQVTVNLEIENDKLM
LVKARREGIGGYPVGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFASSHKVRFI
AIDFEPVVGEILEKVEDGQMGVVLKRMMVRAASQIAERYGVQALVTGEALGQVSSQTLTNLRLIDNASDTLILRPLISHD
KEHIIKVAREIGTEDFAKTMPEYCGVISKSPTVKAIKAKIEEEEGHFDFSILDRVVSEAKNVDIRTIAEQTQEQVTEVET
VAEFDADQVILDIRSNDEQEDKPLKLDQVEVKPLPFYKLSTQFGDLDQSKTYLLYCERGVMSRLQALYLLEQGFTNVKVY
RP
>Mature_482_residues
MKFIIKLFPEITIKSQSVRLRFIKILSTSIRNVLKQYDETLAVVRHWDHIEVRAKDENQRPVIADALTRIPGIHHILEVE
DRDYTDIHHIFEQTLEAYREQLEGKTFCVRVKRRGKQDFNSQDVERYVGGGLNQHIESARVKLSHPQVTVNLEIENDKLM
LVKARREGIGGYPVGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFASSHKVRFI
AIDFEPVVGEILEKVEDGQMGVVLKRMMVRAASQIAERYGVQALVTGEALGQVSSQTLTNLRLIDNASDTLILRPLISHD
KEHIIKVAREIGTEDFAKTMPEYCGVISKSPTVKAIKAKIEEEEGHFDFSILDRVVSEAKNVDIRTIAEQTQEQVTEVET
VAEFDADQVILDIRSNDEQEDKPLKLDQVEVKPLPFYKLSTQFGDLDQSKTYLLYCERGVMSRLQALYLLEQGFTNVKVY
RP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=482, Percent_Identity=82.7800829875519, Blast_Score=814, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_SERP5 (A8GAP5)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477313.1
- ProteinModelPortal:   A8GAP5
- SMR:   A8GAP5
- STRING:   A8GAP5
- GeneID:   5606831
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1081
- eggNOG:   COG0301
- HOGENOM:   HBG646072
- OMA:   GYPIGTQ
- ProtClustDB:   PRK01269
- BioCyc:   SPRO399741:SPRO_1081-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.250.10
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP; SSF52821 Rhodanese-like

EC number: =2.8.1.4

Molecular weight: Translated: 55004; Mature: 55004

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS50206 RHODANESE_3; PS51165 THUMP

Important sites: ACT_SITE 456-456 BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIIKLFPEITIKSQSVRLRFIKILSTSIRNVLKQYDETLAVVRHWDHIEVRAKDENQR
CCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
PVIADALTRIPGIHHILEVEDRDYTDIHHIFEQTLEAYREQLEGKTFCVRVKRRGKQDFN
CHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCC
SQDVERYVGGGLNQHIESARVKLSHPQVTVNLEIENDKLMLVKARREGIGGYPVGTQEDV
HHHHHHHHCCCHHHHHHHHHEEECCCEEEEEEEECCCCEEEEEEHHCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFASSHKVRFI
HHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
AIDFEPVVGEILEKVEDGQMGVVLKRMMVRAASQIAERYGVQALVTGEALGQVSSQTLTN
EEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
LRLIDNASDTLILRPLISHDKEHIIKVAREIGTEDFAKTMPEYCGVISKSPTVKAIKAKI
EEEECCCCCCEEECHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EEEEGHFDFSILDRVVSEAKNVDIRTIAEQTQEQVTEVETVAEFDADQVILDIRSNDEQE
HHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
DKPLKLDQVEVKPLPFYKLSTQFGDLDQSKTYLLYCERGVMSRLQALYLLEQGFTNVKVY
CCCCCCCCEEECCCCCEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEE
RP
CC
>Mature Secondary Structure
MKFIIKLFPEITIKSQSVRLRFIKILSTSIRNVLKQYDETLAVVRHWDHIEVRAKDENQR
CCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
PVIADALTRIPGIHHILEVEDRDYTDIHHIFEQTLEAYREQLEGKTFCVRVKRRGKQDFN
CHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCC
SQDVERYVGGGLNQHIESARVKLSHPQVTVNLEIENDKLMLVKARREGIGGYPVGTQEDV
HHHHHHHHCCCHHHHHHHHHEEECCCEEEEEEEECCCCEEEEEEHHCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFASSHKVRFI
HHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
AIDFEPVVGEILEKVEDGQMGVVLKRMMVRAASQIAERYGVQALVTGEALGQVSSQTLTN
EEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
LRLIDNASDTLILRPLISHDKEHIIKVAREIGTEDFAKTMPEYCGVISKSPTVKAIKAKI
EEEECCCCCCEEECHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EEEEGHFDFSILDRVVSEAKNVDIRTIAEQTQEQVTEVETVAEFDADQVILDIRSNDEQE
HHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
DKPLKLDQVEVKPLPFYKLSTQFGDLDQSKTYLLYCERGVMSRLQALYLLEQGFTNVKVY
CCCCCCCCEEECCCCCEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEE
RP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA