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The map label for this gene is mak [H]

Identifier: 157369279

GI number: 157369279

Start: 1138264

End: 1139172

Strand: Direct

Name: mak [H]

Synonym: Spro_1036

Alternate gene names: 157369279

Gene position: 1138264-1139172 (Clockwise)

Preceding gene: 157369277

Following gene: 157369280

Centisome position: 20.89

GC content: 60.51

Gene sequence:

>909_bases
GTGCGCATTGGTATCGACCTGGGTGGCACCAAAATTGAAGTGATCGCGTTGGCCAACGATGGGCAGGAACTGTTCCGCCA
TCGCATCGCCACGCCGCGCCATGATTATCAGCAAACGCTGGATGCGATTACCGGTCTGGTAAAATTGGCAGAAGAAAATA
CCGGCCAGCAAGGCTCGGTCGGCGTCGGCATTCCCGGCACGCTGTCGCCCTATACCGGGCTGGTCAAAAACGCCAACTCG
GTCTGGCTCAACGGCCAACCGCTGGATAAAGACCTGTCGGCGATGCTGGCCCGTGAAGTGCGTATTGCCAACGATGCCAA
CTGCCTGGCGGTGTCGGAAGCCACCGATGGTGCAGCCGCCGGCAAGCAAACCGTGTTCGCGGTGATTATTGGCACCGGTT
GTGGCGCCGGGGTGGCGATTAACGGCCAGGCGCACTCGGGGGGGAACGGCATCTCCGGCGAGTGGGGACATAACCCGCTG
CCGTGGCTGGATGATGACGAGCTGCGTTTTCGCGCAGAGGTTCCCTGTTACTGCGGTAAGCAGGGGTGTATTGAAACCTT
TATCTCCGGTACCGGCTTTGCCACCGACTATGCTCGCCTGAGCGGTAATCCGCTAAAAGGTCATGAAATCATGACGTTGG
TGGCACAAGGGGATGCAGTGGCGGAGGAGGCCATCAGCCGTTACGAGCTGCGGCTGGCGAAATCGCTGGCGCACGTGATC
AATATTTTTGATCCGGACGTCGTAGTGCTGGGCGGGGGGATGAGCAACGTGGATCGGTTGTACCAGCGGGTGCCGCAGTT
GGTGAAATCCTGGGTGTTCGGTGGCGAATGCGAAACCCCGATCCGCAAGGCGGCACACGGCGATTCCAGCGGCGTGCGCG
GCGCGGCGTGGCTGTGGCCGCAACTTTAA

Upstream 100 bases:

>100_bases
GGAAATTGCCATCACCGTCACACTCGGCGCTGATTGAGTGATCCTGGCAACTGAATATATTAGGCTGTGGCAACGTTGTT
GTGCATTGAGGAGAAAAATC

Downstream 100 bases:

>100_bases
TTACCGTTGCAAAAAAGCGCGGGCCTGCTCGCGCTTTAATCAATCTGCTGTACAAAAACTGGACTCCCCTCTATGCTTTA
GCTAACTTAGCTAAGGAGAT

Product: ROK family protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRIGIDLGGTKIEVIALANDGQELFRHRIATPRHDYQQTLDAITGLVKLAEENTGQQGSVGVGIPGTLSPYTGLVKNANS
VWLNGQPLDKDLSAMLAREVRIANDANCLAVSEATDGAAAGKQTVFAVIIGTGCGAGVAINGQAHSGGNGISGEWGHNPL
PWLDDDELRFRAEVPCYCGKQGCIETFISGTGFATDYARLSGNPLKGHEIMTLVAQGDAVAEEAISRYELRLAKSLAHVI
NIFDPDVVVLGGGMSNVDRLYQRVPQLVKSWVFGGECETPIRKAAHGDSSGVRGAAWLWPQL

Sequences:

>Translated_302_residues
MRIGIDLGGTKIEVIALANDGQELFRHRIATPRHDYQQTLDAITGLVKLAEENTGQQGSVGVGIPGTLSPYTGLVKNANS
VWLNGQPLDKDLSAMLAREVRIANDANCLAVSEATDGAAAGKQTVFAVIIGTGCGAGVAINGQAHSGGNGISGEWGHNPL
PWLDDDELRFRAEVPCYCGKQGCIETFISGTGFATDYARLSGNPLKGHEIMTLVAQGDAVAEEAISRYELRLAKSLAHVI
NIFDPDVVVLGGGMSNVDRLYQRVPQLVKSWVFGGECETPIRKAAHGDSSGVRGAAWLWPQL
>Mature_302_residues
MRIGIDLGGTKIEVIALANDGQELFRHRIATPRHDYQQTLDAITGLVKLAEENTGQQGSVGVGIPGTLSPYTGLVKNANS
VWLNGQPLDKDLSAMLAREVRIANDANCLAVSEATDGAAAGKQTVFAVIIGTGCGAGVAINGQAHSGGNGISGEWGHNPL
PWLDDDELRFRAEVPCYCGKQGCIETFISGTGFATDYARLSGNPLKGHEIMTLVAQGDAVAEEAISRYELRLAKSLAHVI
NIFDPDVVVLGGGMSNVDRLYQRVPQLVKSWVFGGECETPIRKAAHGDSSGVRGAAWLWPQL

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=301, Percent_Identity=78.0730897009967, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1787363, Length=301, Percent_Identity=40.1993355481728, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1786891, Length=230, Percent_Identity=25.6521739130435, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790522, Length=261, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787878, Length=208, Percent_Identity=25.9615384615385, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32018; Mature: 32018

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIDLGGTKIEVIALANDGQELFRHRIATPRHDYQQTLDAITGLVKLAEENTGQQGSV
CEEEEECCCCEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
GVGIPGTLSPYTGLVKNANSVWLNGQPLDKDLSAMLAREVRIANDANCLAVSEATDGAAA
ECCCCCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
GKQTVFAVIIGTGCGAGVAINGQAHSGGNGISGEWGHNPLPWLDDDELRFRAEVPCYCGK
CCCEEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
QGCIETFISGTGFATDYARLSGNPLKGHEIMTLVAQGDAVAEEAISRYELRLAKSLAHVI
HHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
NIFDPDVVVLGGGMSNVDRLYQRVPQLVKSWVFGGECETPIRKAAHGDSSGVRGAAWLWP
HHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEEECCC
QL
CC
>Mature Secondary Structure
MRIGIDLGGTKIEVIALANDGQELFRHRIATPRHDYQQTLDAITGLVKLAEENTGQQGSV
CEEEEECCCCEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
GVGIPGTLSPYTGLVKNANSVWLNGQPLDKDLSAMLAREVRIANDANCLAVSEATDGAAA
ECCCCCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
GKQTVFAVIIGTGCGAGVAINGQAHSGGNGISGEWGHNPLPWLDDDELRFRAEVPCYCGK
CCCEEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
QGCIETFISGTGFATDYARLSGNPLKGHEIMTLVAQGDAVAEEAISRYELRLAKSLAHVI
HHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
NIFDPDVVVLGGGMSNVDRLYQRVPQLVKSWVFGGECETPIRKAAHGDSSGVRGAAWLWP
HHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEEECCC
QL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]