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The map label for this gene is pcaK [H]

Identifier: 157369052

GI number: 157369052

Start: 902176

End: 903336

Strand: Reverse

Name: pcaK [H]

Synonym: Spro_0807

Alternate gene names: 157369052

Gene position: 903336-902176 (Counterclockwise)

Preceding gene: 157369054

Following gene: 157369051

Centisome position: 16.58

GC content: 57.62

Gene sequence:

>1161_bases
ATGGCTCATTCCACCGAGTTGAATATTCAGCAGGCCATCGACGACAGCAAATTCTCGCTGTTTCACTGGACCTTGATCAT
TTTGGGATTCCTGATCCTGGCCATTGATGGCTTTGACACTGCGGCCATGGGGTATATCGCCCCTTCGGTCGCCAAAGACT
GGGGCATTGTGAAACAGGATCTCGGCCCGGTACTGAGCGCCGCCCTGTTGGGCCTGTCACTCGGTGCACTGGTTGCCGGA
CCGATCTCCGACCGTATCGGTCGCAAACGGGTGTTGGTGTTTTCATGCCTGTTCTTCGGTCTTTCAAGCCTGGCCACCGC
CTATGCCGGCTCACTCAATAGCCTGACGCTGTGGCGCTTTCTCACCGGTCTGGGGCTGGGAGCGGCAATGCCGAACGCCA
TCACGCTGATTTCCGAATATGCACCGCAGCGCTGCCGTTCATTGGCGATTAACACCATGTACTGTGGTTTCCCTCTGGGT
GCCGCAGGCGGCGGAGCGATTTCGTCATGGCTGATCCCGGCCTATGGCTGGCACAGCGTGCTGTTGCTGGGAGCGATCGC
TCCGCTGGTGCTGACGGTATTGTTGATCCTGTTCCTGCCGGAATCGGTGAAGTACATGGTTAACCGCGGCAAAGACGCGA
TCAAAATCAAACGCATTGCCCAGCGCTTTGTCAGCCAAAATCTCGATAACGTCACGCGTTTCTATCTGTATGAAGAGAAA
CTGGCACAGTCGAAAACCAGCGTCGGCCTGTTGTTCACCCGGCCTTATCTGACCGGCACGCTGATGCTGTGGCTGACCTA
CTTTATGGGGCTGGTAATTTATTACGTGCTGCTGAGCTGGATGCCTATTCTGATGCAGGGGCTGGGCTATCAGTTGGAGC
AGTCGGCGATCCTGACCTCGCTGTTCACCTTCGGCGGCACCCTTGGCATTCTGGTGGCCGGCTGGCTGATGGATCGCTGG
AACGCCCATAAGGTGGTGTCGAGCGGCTTTGTCATTACCGCGCTGCTGATCGTGGCGATGGCCACCGAGGACAGCCATAT
CGTGCTGCTGGGCACCTTTATCTTCCTGATGGGGATTACCATGAACGGCGCGCAGTCCGGCCTGCAAACGCTGGCGGCGA
CCTTCTACCCCACCCACAGTCGCGCAACCGGCATTGCCTAG

Upstream 100 bases:

>100_bases
ATAAATCGCTCGCACGCAGATGTATATACAGGCAAGCACAAGCCGGACGCCAGTTTCGGCGGTTCATCCACCACAATCAC
ATCTGCGTTTAGAGGGTGCC

Downstream 100 bases:

>100_bases
ATGCAGGGTATCGGCCGCTTTGGTGGCGTGGCTGGTACCATGATGGGAGCGCAGCTGCTGGCCATGCAGTGGGAAGTGCA
GAGTATCTTGATGTTCCTGT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MAHSTELNIQQAIDDSKFSLFHWTLIILGFLILAIDGFDTAAMGYIAPSVAKDWGIVKQDLGPVLSAALLGLSLGALVAG
PISDRIGRKRVLVFSCLFFGLSSLATAYAGSLNSLTLWRFLTGLGLGAAMPNAITLISEYAPQRCRSLAINTMYCGFPLG
AAGGGAISSWLIPAYGWHSVLLLGAIAPLVLTVLLILFLPESVKYMVNRGKDAIKIKRIAQRFVSQNLDNVTRFYLYEEK
LAQSKTSVGLLFTRPYLTGTLMLWLTYFMGLVIYYVLLSWMPILMQGLGYQLEQSAILTSLFTFGGTLGILVAGWLMDRW
NAHKVVSSGFVITALLIVAMATEDSHIVLLGTFIFLMGITMNGAQSGLQTLAATFYPTHSRATGIA

Sequences:

>Translated_386_residues
MAHSTELNIQQAIDDSKFSLFHWTLIILGFLILAIDGFDTAAMGYIAPSVAKDWGIVKQDLGPVLSAALLGLSLGALVAG
PISDRIGRKRVLVFSCLFFGLSSLATAYAGSLNSLTLWRFLTGLGLGAAMPNAITLISEYAPQRCRSLAINTMYCGFPLG
AAGGGAISSWLIPAYGWHSVLLLGAIAPLVLTVLLILFLPESVKYMVNRGKDAIKIKRIAQRFVSQNLDNVTRFYLYEEK
LAQSKTSVGLLFTRPYLTGTLMLWLTYFMGLVIYYVLLSWMPILMQGLGYQLEQSAILTSLFTFGGTLGILVAGWLMDRW
NAHKVVSSGFVITALLIVAMATEDSHIVLLGTFIFLMGITMNGAQSGLQTLAATFYPTHSRATGIA
>Mature_385_residues
AHSTELNIQQAIDDSKFSLFHWTLIILGFLILAIDGFDTAAMGYIAPSVAKDWGIVKQDLGPVLSAALLGLSLGALVAGP
ISDRIGRKRVLVFSCLFFGLSSLATAYAGSLNSLTLWRFLTGLGLGAAMPNAITLISEYAPQRCRSLAINTMYCGFPLGA
AGGGAISSWLIPAYGWHSVLLLGAIAPLVLTVLLILFLPESVKYMVNRGKDAIKIKRIAQRFVSQNLDNVTRFYLYEEKL
AQSKTSVGLLFTRPYLTGTLMLWLTYFMGLVIYYVLLSWMPILMQGLGYQLEQSAILTSLFTFGGTLGILVAGWLMDRWN
AHKVVSSGFVITALLIVAMATEDSHIVLLGTFIFLMGITMNGAQSGLQTLAATFYPTHSRATGIA

Specific function: Could Be A Transporter For 3-Phenylpropionate (Hydrocinnamic Acid). [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=404, Percent_Identity=24.5049504950495, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI7662270, Length=338, Percent_Identity=23.0769230769231, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI166064021, Length=236, Percent_Identity=27.9661016949153, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI73695465, Length=343, Percent_Identity=22.7405247813411, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI216548223, Length=203, Percent_Identity=26.1083743842365, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI87081723, Length=363, Percent_Identity=35.2617079889807, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87082159, Length=380, Percent_Identity=26.5789473684211, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082231, Length=385, Percent_Identity=24.1558441558442, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788074, Length=256, Percent_Identity=26.171875, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788068, Length=392, Percent_Identity=24.234693877551, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786229, Length=381, Percent_Identity=23.0971128608924, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789312, Length=305, Percent_Identity=23.6065573770492, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789207, Length=281, Percent_Identity=24.5551601423488, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87082404, Length=181, Percent_Identity=26.5193370165746, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789152, Length=312, Percent_Identity=22.7564102564103, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=282, Percent_Identity=23.0496453900709, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640198, Length=309, Percent_Identity=25.5663430420712, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640196, Length=309, Percent_Identity=25.5663430420712, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640200, Length=309, Percent_Identity=25.5663430420712, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922874, Length=388, Percent_Identity=23.4536082474227, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24646916, Length=343, Percent_Identity=25.6559766763848, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41842; Mature: 41711

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHSTELNIQQAIDDSKFSLFHWTLIILGFLILAIDGFDTAAMGYIAPSVAKDWGIVKQD
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LGPVLSAALLGLSLGALVAGPISDRIGRKRVLVFSCLFFGLSSLATAYAGSLNSLTLWRF
HHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LTGLGLGAAMPNAITLISEYAPQRCRSLAINTMYCGFPLGAAGGGAISSWLIPAYGWHSV
HHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHCCCCHHHH
LLLGAIAPLVLTVLLILFLPESVKYMVNRGKDAIKIKRIAQRFVSQNLDNVTRFYLYEEK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LAQSKTSVGLLFTRPYLTGTLMLWLTYFMGLVIYYVLLSWMPILMQGLGYQLEQSAILTS
HHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LFTFGGTLGILVAGWLMDRWNAHKVVSSGFVITALLIVAMATEDSHIVLLGTFIFLMGIT
HHHHCCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCC
MNGAQSGLQTLAATFYPTHSRATGIA
CCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AHSTELNIQQAIDDSKFSLFHWTLIILGFLILAIDGFDTAAMGYIAPSVAKDWGIVKQD
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH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CCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]