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The map label for this gene is hemL

Identifier: 157369026

GI number: 157369026

Start: 870367

End: 871656

Strand: Reverse

Name: hemL

Synonym: Spro_0781

Alternate gene names: 157369026

Gene position: 871656-870367 (Counterclockwise)

Preceding gene: 157369029

Following gene: 157369024

Centisome position: 16.0

GC content: 59.92

Gene sequence:

>1290_bases
ATGAGCTTACACAGCAAGTCCGAAAGTCTGTATGCCCAGGCAAAACAGGTAATCCCAGGCGGGGTTAACTCTCCGGTGCG
TGCATTCAACGGCGTTGGCGGTGTGCCGCTGTTTATCGAACGGGCGGACGGGGCTTACCTGTTCGACGTCGACGGCAAAG
CCTATATCGACTACGTCGGTTCCTGGGGCCCAATGGTATTGGGGCACAACCACCCGGCGATCCGCAACGCGGTCATCGAA
GCGGCGCAGCGCGGCCTGAGCTTTGGCGCGCCCACCGAGATGGAAGTTAAAATGGCGGAGCTGGTCACCGAACTGGTGCC
GACCATGGATATGGTACGCATGGTGAACTCGGGGACCGAAGCCACCATGAGCGCCATTCGTCTGGCGCGCGGCTACACCA
ACCGCGATAAAATCATCAAGTTTGAAGGCTGCTACCACGGCCACGCGGACTGCCTGCTGGTGAAAGCCGGTTCTGGCGCG
CTGACCCTGGGCCAGCCAAACTCCCCGGGCGTGCCGGCTGACTTTGCCAAGCACACTCTGACCTGCACCTTTAACGATCT
GGACTCGGTACGCACCGCATTTGAGCAGTACCCTTCCGAGATCGCCTGCATCATCGTCGAACCGGTCGCCGGTAACATGA
ACTGCGTACCGCCACTGCCAGAATTCCTGCCGGGCCTGCGTGCGTTGTGCGACAAGTACGGCGCACTGCTGATTATCGAC
GAAGTCATGACCGGCTTCCGCGTGGCGCTGGCCGGGGCACAATCCTATTACGATGTGGTGCCGGACCTCACCTGTCTGGG
TAAAATCATCGGCGGCGGCATGCCTGTTGGCGCATTCGGCGGCCGTCGTGAAGTGATGGCAGCGCTGGCACCAACCGGCC
CGGTCTATCAGGCAGGCACGCTGTCCGGTAACCCGATTGCGATGGCGGCAGGCTTTGCCTGCCTGACCGAAGTTTCGCAG
GTCGGTGTCCACCAGACGCTGACCGAACTGACCGATATGCTGGCAGCCGGCCTGTTGCACGCGGCTAAAGAAGAAAACGT
CGCGCTGGTGGTGAACCACGTTGGCGGCATGTTCGGCCTGTTCTTTACCGATGCGCTGTCAGTCACCAGTTATCAGGACG
TGATGCAGTGCGATGTAGAGCGCTTCAAGCGTTTCTTCCATCTGATGCTGGAGGAAGGGGTTTATCTGGCGCCTTCTGCC
TTCGAGGCCGGCTTTATGTCCATTGCGCACAGCAAAGAAGACATTCAGCGCACTATAGATGCCGCGCGCCGCTGCTTCGC
CAAACTGTAA

Upstream 100 bases:

>100_bases
CCCCTCTAAATAGGTATCTGACTGTGATCGTTTCATATCGGCGAACACTTCACTAGAATAGCGCGGTCAAATCATTCAAA
CAGAATCCAGGAGCCGATTT

Downstream 100 bases:

>100_bases
GTTTCGGCTATCCAATAAAAACGGGCGCTCAGCGCCCGTTTTTATTTCCAAATTTCGCCGTCAGAGCAAAGTACCGTAAA
TCGTCAGCAGGGCTACCACC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSLHSKSESLYAQAKQVIPGGVNSPVRAFNGVGGVPLFIERADGAYLFDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIE
AAQRGLSFGAPTEMEVKMAELVTELVPTMDMVRMVNSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHADCLLVKAGSGA
LTLGQPNSPGVPADFAKHTLTCTFNDLDSVRTAFEQYPSEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDKYGALLIID
EVMTGFRVALAGAQSYYDVVPDLTCLGKIIGGGMPVGAFGGRREVMAALAPTGPVYQAGTLSGNPIAMAAGFACLTEVSQ
VGVHQTLTELTDMLAAGLLHAAKEENVALVVNHVGGMFGLFFTDALSVTSYQDVMQCDVERFKRFFHLMLEEGVYLAPSA
FEAGFMSIAHSKEDIQRTIDAARRCFAKL

Sequences:

>Translated_429_residues
MSLHSKSESLYAQAKQVIPGGVNSPVRAFNGVGGVPLFIERADGAYLFDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIE
AAQRGLSFGAPTEMEVKMAELVTELVPTMDMVRMVNSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHADCLLVKAGSGA
LTLGQPNSPGVPADFAKHTLTCTFNDLDSVRTAFEQYPSEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDKYGALLIID
EVMTGFRVALAGAQSYYDVVPDLTCLGKIIGGGMPVGAFGGRREVMAALAPTGPVYQAGTLSGNPIAMAAGFACLTEVSQ
VGVHQTLTELTDMLAAGLLHAAKEENVALVVNHVGGMFGLFFTDALSVTSYQDVMQCDVERFKRFFHLMLEEGVYLAPSA
FEAGFMSIAHSKEDIQRTIDAARRCFAKL
>Mature_428_residues
SLHSKSESLYAQAKQVIPGGVNSPVRAFNGVGGVPLFIERADGAYLFDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEA
AQRGLSFGAPTEMEVKMAELVTELVPTMDMVRMVNSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHADCLLVKAGSGAL
TLGQPNSPGVPADFAKHTLTCTFNDLDSVRTAFEQYPSEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDKYGALLIIDE
VMTGFRVALAGAQSYYDVVPDLTCLGKIIGGGMPVGAFGGRREVMAALAPTGPVYQAGTLSGNPIAMAAGFACLTEVSQV
GVHQTLTELTDMLAAGLLHAAKEENVALVVNHVGGMFGLFFTDALSVTSYQDVMQCDVERFKRFFHLMLEEGVYLAPSAF
EAGFMSIAHSKEDIQRTIDAARRCFAKL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=315, Percent_Identity=29.2063492063492, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI13994255, Length=378, Percent_Identity=27.5132275132275, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI284507298, Length=263, Percent_Identity=28.5171102661597, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=88.2352941176471, Blast_Score=779, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=381, Percent_Identity=31.758530183727, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788044, Length=283, Percent_Identity=34.2756183745583, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI145693181, Length=309, Percent_Identity=31.3915857605178, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787560, Length=343, Percent_Identity=30.9037900874636, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789759, Length=282, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1786991, Length=383, Percent_Identity=27.4151436031332, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI25144271, Length=343, Percent_Identity=29.1545189504373, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71992977, Length=358, Percent_Identity=28.2122905027933, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI32564660, Length=339, Percent_Identity=26.5486725663717, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144274, Length=183, Percent_Identity=31.6939890710383, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323470, Length=413, Percent_Identity=27.6029055690073, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324432, Length=348, Percent_Identity=26.4367816091954, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21357415, Length=308, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356575, Length=329, Percent_Identity=28.8753799392097, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_SERP5 (A8G9U7)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477015.1
- ProteinModelPortal:   A8G9U7
- SMR:   A8G9U7
- STRING:   A8G9U7
- GeneID:   5604207
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0781
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   DKVIKFE
- ProtClustDB:   PRK00062
- BioCyc:   SPRO399741:SPRO_0781-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45872; Mature: 45741

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLHSKSESLYAQAKQVIPGGVNSPVRAFNGVGGVPLFIERADGAYLFDVDGKAYIDYVG
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCEEEEECCCCEEEEEEC
SWGPMVLGHNHPAIRNAVIEAAQRGLSFGAPTEMEVKMAELVTELVPTMDMVRMVNSGTE
CCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
ATMSAIRLARGYTNRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTL
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCHHHHCCEE
TCTFNDLDSVRTAFEQYPSEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDKYGALLIID
EEEECCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHH
EVMTGFRVALAGAQSYYDVVPDLTCLGKIIGGGMPVGAFGGRREVMAALAPTGPVYQAGT
HHHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPIAMAAGFACLTEVSQVGVHQTLTELTDMLAAGLLHAAKEENVALVVNHVGGMFGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
FFTDALSVTSYQDVMQCDVERFKRFFHLMLEEGVYLAPSAFEAGFMSIAHSKEDIQRTID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHH
AARRCFAKL
HHHHHHHCC
>Mature Secondary Structure 
SLHSKSESLYAQAKQVIPGGVNSPVRAFNGVGGVPLFIERADGAYLFDVDGKAYIDYVG
CCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCEEEEECCCCEEEEEEC
SWGPMVLGHNHPAIRNAVIEAAQRGLSFGAPTEMEVKMAELVTELVPTMDMVRMVNSGTE
CCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
ATMSAIRLARGYTNRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTL
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCHHHHCCEE
TCTFNDLDSVRTAFEQYPSEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDKYGALLIID
EEEECCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHH
EVMTGFRVALAGAQSYYDVVPDLTCLGKIIGGGMPVGAFGGRREVMAALAPTGPVYQAGT
HHHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPIAMAAGFACLTEVSQVGVHQTLTELTDMLAAGLLHAAKEENVALVVNHVGGMFGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
FFTDALSVTSYQDVMQCDVERFKRFFHLMLEEGVYLAPSAFEAGFMSIAHSKEDIQRTID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHH
AARRCFAKL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA