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The map label for this gene is polB [H]

Identifier: 157368976

GI number: 157368976

Start: 811689

End: 814052

Strand: Reverse

Name: polB [H]

Synonym: Spro_0731

Alternate gene names: 157368976

Gene position: 814052-811689 (Counterclockwise)

Preceding gene: 157368978

Following gene: 157368975

Centisome position: 14.94

GC content: 58.8

Gene sequence:

>2364_bases
ATGTCACTCCAACAGGGTTTCTTGCTCACCCGCCACTGGCGTGATACGCCCGCCGGCACCGAGGTAGAGTTTTGGCTGGC
GACCGACAGCGGCCCGCAGCAACTTCGCCTGGCACCGCAGACCTCGGTCGCCTTTATCCCGGCCGAACAGCGCCAACGCA
CCGAACTGTTGCTGCGGGAAGAGCGGCAATGGGAGCTAAAGCCGTTGCCGCTGACCGATTTCCATCATCGCCCGGTGTTG
GGGCTGTATTGCCGTCAGCATCGTCAGTTGATCAAACTGGAAAGGCTGCTGAAAGACGGTGGCGTTAACCTCTATGAGGC
CGATATTCGCCCGCCGGAACGCTACCTGATGGAGCGTTTTATCACCGCACCGGTCTGGTTCAGCGGCCAGGCGAACGGCA
ATGGCCCGTTGATCAACGGCCAAATGAAACCGGCACCGGAATACCGCCCGACCTTGAAACTGGTCTCGCTGGACATTGAA
ACCACCGCCCACGGCGAGCTGTACTCGATCGCGCTGGAAGGTTGCGGTCAGCGCCAGGTGTACATGCTCGGGCCAGCCAA
CGGGGGCAATGAAGCGCTGGATTTTGACCTGGAGTACTGCGTCAGCCGCCCGCAGATGCTGGAACGGCTGAATCAGTGGC
TGGAGCGGCACGATCCCGATGCCATCATTGGCTGGAACCTGGTGCAGTTCGATTTACGGGTGCTGCAAAAACACGCTGAG
CGTTACCAAATTCCGCTAAAACTGGGCCGCGGTGGCAACCTGCTCGAATGGCGTGAACACGGCTTCAAACAGAACCACTT
TTTCGCCTCCGCTGCCGGTCGGTTGATTATCGACGGTATCGAGGCGCTGAAATCCGCCACCTGGAACTTCCCTTCTTTCA
GCCTCGAACATGTGTCGCAGACGCTGTTGGGCGAAGGCAAGTCGATCGACAATCCCTACCAGCGAATGGCAGAAATCGAC
CGGCGCTTCGCCGAAGATAAACCGGCGCTGGCACGCTACAACCTCAAAGACTGCGAACTGGTAACGCAAATCTTCGCCAA
GACCGATTTGCTGACCTTCCTGCTGGAGCGCGCCACGGTGACCGGATTGGCGGCCGACCGCAGCGGCGGTTCCGTCGCCG
CATTCAGCAATCTGTATATGCCACGCATGCACCGGCTGGGTTTTGTTGCCCCCAACCTTGGCGAGCAGCCGGAAGAGCAC
AGCCCCGGCGGTTTTGTGATGGATTCGCAGCCCGGCCTGTATGACTCGGTGCTGGTGCTCGATTACAAGAGCCTGTACCC
GTCGATCATCCGTACCTTCCTCATCGACCCGGTCGGACTGGTGGAAGGCATGCAACACCCCGGCGATGCCGATTCGGTGC
CCGGTTTCCGCCATGCCCGTTTCTCGCGCAGCAAACACTGCCTGCCGGCGATCGTCGAGCAGATCTGGCAGGGTCGCGAA
GCAGCCAAGCGTCAGCAGAACAAAGCCTTGTCCCAGGCATTGAAGATCATCATGAACGCGCTGTACGGCGTATTGGGATC
GAGCGGTTGCCGTTTCTTCGATCCCCGTCTGGCCTCGTCGATTACCCTGCGCGGCCATGAGATCATGCGCCAGACGCGCG
AACTGATCGAGGCTCAGGGTTATCAGGTGATCTACGGCGATACCGACTCCACCTTCGTCTGGCTCAAACAGCCGCATGAC
GAGCAGCAGGCAGCGCAAATAGGCCGGACATTGGTCGAACAGGTGAACCAATGGTGGCAACAGCATTTGCAGCAACAGTA
TGGGCTGGAGAATGCGCTGGAACTGGAGTTCGAAACCCATTACTCACGTTTCCTGATGCCCACCATTCGCGGTGCCGAAC
AGGGCAGTAAAAAACGTTACGCCGGGCTGATCGCCAAACCGGACGGCAGTGAAGAGATGGTGTACAAAGGGCTGGAAACG
GTGCGCACCGACTGGACCCCGCTGGCGCAGCAGTTCCAGCAGCAGCTTTACCAACGTATTTTCAAACGCCAGCCGTATCA
GGATTTTGTGCGTGAATACGTTAGCCAAACGCTTAACGGCGATTTCGACGATCGGCTGGTCTACCGCAAGCGCCTGCGCA
GAAAGCTGGACGAATATCAACGCAACGTCCCACCACATGTCCGCGCAGCCCGCATCGCCGACGACTATAATCGTCAGCAG
GGACGACCACTGCAATACCAGAACGGCGGCTGGATCAGCTATGTGATGACCGTGGCCGGGCCGGAACCGCTGGAAACCCA
GCAGTCGGCGATTGATTACCCCCATTATCTGGAGCGCCAGTTGGAACCGGTGGCAGACGCTATACTGCCATTTTTGCATG
ACGATTTTACTACGCTGGTGACCGGGCAGATGGGCCTGTTTTAG

Upstream 100 bases:

>100_bases
GTTTTTGGCAGAACTGTGCGGCAATGGACAATCACTGTATAAAAACCCATACTATATTTATATTTTCTCCCTTATAGCTT
AGCTTGTCAGGAACAGCGCG

Downstream 100 bases:

>100_bases
CATTCGGCCCACTCTGCGGGCTTTGGTGAAGGGTTAAAAAAATTCATCGGGTTTTGCAGGTGACGAACGCGCCGCCTTCC
ATTACCATAGCGCCCTTCCC

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 787; Mature: 786

Protein sequence:

>787_residues
MSLQQGFLLTRHWRDTPAGTEVEFWLATDSGPQQLRLAPQTSVAFIPAEQRQRTELLLREERQWELKPLPLTDFHHRPVL
GLYCRQHRQLIKLERLLKDGGVNLYEADIRPPERYLMERFITAPVWFSGQANGNGPLINGQMKPAPEYRPTLKLVSLDIE
TTAHGELYSIALEGCGQRQVYMLGPANGGNEALDFDLEYCVSRPQMLERLNQWLERHDPDAIIGWNLVQFDLRVLQKHAE
RYQIPLKLGRGGNLLEWREHGFKQNHFFASAAGRLIIDGIEALKSATWNFPSFSLEHVSQTLLGEGKSIDNPYQRMAEID
RRFAEDKPALARYNLKDCELVTQIFAKTDLLTFLLERATVTGLAADRSGGSVAAFSNLYMPRMHRLGFVAPNLGEQPEEH
SPGGFVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMQHPGDADSVPGFRHARFSRSKHCLPAIVEQIWQGRE
AAKRQQNKALSQALKIIMNALYGVLGSSGCRFFDPRLASSITLRGHEIMRQTRELIEAQGYQVIYGDTDSTFVWLKQPHD
EQQAAQIGRTLVEQVNQWWQQHLQQQYGLENALELEFETHYSRFLMPTIRGAEQGSKKRYAGLIAKPDGSEEMVYKGLET
VRTDWTPLAQQFQQQLYQRIFKRQPYQDFVREYVSQTLNGDFDDRLVYRKRLRRKLDEYQRNVPPHVRAARIADDYNRQQ
GRPLQYQNGGWISYVMTVAGPEPLETQQSAIDYPHYLERQLEPVADAILPFLHDDFTTLVTGQMGLF

Sequences:

>Translated_787_residues
MSLQQGFLLTRHWRDTPAGTEVEFWLATDSGPQQLRLAPQTSVAFIPAEQRQRTELLLREERQWELKPLPLTDFHHRPVL
GLYCRQHRQLIKLERLLKDGGVNLYEADIRPPERYLMERFITAPVWFSGQANGNGPLINGQMKPAPEYRPTLKLVSLDIE
TTAHGELYSIALEGCGQRQVYMLGPANGGNEALDFDLEYCVSRPQMLERLNQWLERHDPDAIIGWNLVQFDLRVLQKHAE
RYQIPLKLGRGGNLLEWREHGFKQNHFFASAAGRLIIDGIEALKSATWNFPSFSLEHVSQTLLGEGKSIDNPYQRMAEID
RRFAEDKPALARYNLKDCELVTQIFAKTDLLTFLLERATVTGLAADRSGGSVAAFSNLYMPRMHRLGFVAPNLGEQPEEH
SPGGFVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMQHPGDADSVPGFRHARFSRSKHCLPAIVEQIWQGRE
AAKRQQNKALSQALKIIMNALYGVLGSSGCRFFDPRLASSITLRGHEIMRQTRELIEAQGYQVIYGDTDSTFVWLKQPHD
EQQAAQIGRTLVEQVNQWWQQHLQQQYGLENALELEFETHYSRFLMPTIRGAEQGSKKRYAGLIAKPDGSEEMVYKGLET
VRTDWTPLAQQFQQQLYQRIFKRQPYQDFVREYVSQTLNGDFDDRLVYRKRLRRKLDEYQRNVPPHVRAARIADDYNRQQ
GRPLQYQNGGWISYVMTVAGPEPLETQQSAIDYPHYLERQLEPVADAILPFLHDDFTTLVTGQMGLF
>Mature_786_residues
SLQQGFLLTRHWRDTPAGTEVEFWLATDSGPQQLRLAPQTSVAFIPAEQRQRTELLLREERQWELKPLPLTDFHHRPVLG
LYCRQHRQLIKLERLLKDGGVNLYEADIRPPERYLMERFITAPVWFSGQANGNGPLINGQMKPAPEYRPTLKLVSLDIET
TAHGELYSIALEGCGQRQVYMLGPANGGNEALDFDLEYCVSRPQMLERLNQWLERHDPDAIIGWNLVQFDLRVLQKHAER
YQIPLKLGRGGNLLEWREHGFKQNHFFASAAGRLIIDGIEALKSATWNFPSFSLEHVSQTLLGEGKSIDNPYQRMAEIDR
RFAEDKPALARYNLKDCELVTQIFAKTDLLTFLLERATVTGLAADRSGGSVAAFSNLYMPRMHRLGFVAPNLGEQPEEHS
PGGFVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMQHPGDADSVPGFRHARFSRSKHCLPAIVEQIWQGREA
AKRQQNKALSQALKIIMNALYGVLGSSGCRFFDPRLASSITLRGHEIMRQTRELIEAQGYQVIYGDTDSTFVWLKQPHDE
QQAAQIGRTLVEQVNQWWQQHLQQQYGLENALELEFETHYSRFLMPTIRGAEQGSKKRYAGLIAKPDGSEEMVYKGLETV
RTDWTPLAQQFQQQLYQRIFKRQPYQDFVREYVSQTLNGDFDDRLVYRKRLRRKLDEYQRNVPPHVRAARIADDYNRQQG
RPLQYQNGGWISYVMTVAGPEPLETQQSAIDYPHYLERQLEPVADAILPFLHDDFTTLVTGQMGLF

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=659, Percent_Identity=27.3141122913505, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI106507301, Length=657, Percent_Identity=24.8097412480974, Blast_Score=136, Evalue=1e-31,
Organism=Escherichia coli, GI1786246, Length=784, Percent_Identity=71.0459183673469, Blast_Score=1155, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559488, Length=771, Percent_Identity=25.6809338521401, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI32565317, Length=634, Percent_Identity=23.5015772870662, Blast_Score=125, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI86563326, Length=412, Percent_Identity=24.5145631067961, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320101, Length=693, Percent_Identity=24.6753246753247, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324227, Length=592, Percent_Identity=25.6756756756757, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24664937, Length=641, Percent_Identity=26.9890795631825, Blast_Score=131, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24648780, Length=641, Percent_Identity=22.1528861154446, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 90356; Mature: 90225

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQQGFLLTRHWRDTPAGTEVEFWLATDSGPQQLRLAPQTSVAFIPAEQRQRTELLLRE
CCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCEEECCCCCEEEECCHHHHHHHHHHHH
ERQWELKPLPLTDFHHRPVLGLYCRQHRQLIKLERLLKDGGVNLYEADIRPPERYLMERF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHH
ITAPVWFSGQANGNGPLINGQMKPAPEYRPTLKLVSLDIETTAHGELYSIALEGCGQRQV
HHCCEEECCCCCCCCCEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCEE
YMLGPANGGNEALDFDLEYCVSRPQMLERLNQWLERHDPDAIIGWNLVQFDLRVLQKHAE
EEEECCCCCCCCEECCHHHHCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHH
RYQIPLKLGRGGNLLEWREHGFKQNHFFASAAGRLIIDGIEALKSATWNFPSFSLEHVSQ
HEECCEEECCCCCCHHHHHCCCCCCCEEHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
TLLGEGKSIDNPYQRMAEIDRRFAEDKPALARYNLKDCELVTQIFAKTDLLTFLLERATV
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TGLAADRSGGSVAAFSNLYMPRMHRLGFVAPNLGEQPEEHSPGGFVMDSQPGLYDSVLVL
HHEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHH
DYKSLYPSIIRTFLIDPVGLVEGMQHPGDADSVPGFRHARFSRSKHCLPAIVEQIWQGRE
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCHH
AAKRQQNKALSQALKIIMNALYGVLGSSGCRFFDPRLASSITLRGHEIMRQTRELIEAQG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHEEECHHHHHHHHHHHHHHCC
YQVIYGDTDSTFVWLKQPHDEQQAAQIGRTLVEQVNQWWQQHLQQQYGLENALELEFETH
CEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEHHH
YSRFLMPTIRGAEQGSKKRYAGLIAKPDGSEEMVYKGLETVRTDWTPLAQQFQQQLYQRI
HHHHHHHHHCCCCCCCCHHHCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FKRQPYQDFVREYVSQTLNGDFDDRLVYRKRLRRKLDEYQRNVPPHVRAARIADDYNRQQ
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
GRPLQYQNGGWISYVMTVAGPEPLETQQSAIDYPHYLERQLEPVADAILPFLHDDFTTLV
CCCCEECCCCEEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
TGQMGLF
CCCCCCC
>Mature Secondary Structure 
SLQQGFLLTRHWRDTPAGTEVEFWLATDSGPQQLRLAPQTSVAFIPAEQRQRTELLLRE
CCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCEEECCCCCEEEECCHHHHHHHHHHHH
ERQWELKPLPLTDFHHRPVLGLYCRQHRQLIKLERLLKDGGVNLYEADIRPPERYLMERF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHH
ITAPVWFSGQANGNGPLINGQMKPAPEYRPTLKLVSLDIETTAHGELYSIALEGCGQRQV
HHCCEEECCCCCCCCCEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCEE
YMLGPANGGNEALDFDLEYCVSRPQMLERLNQWLERHDPDAIIGWNLVQFDLRVLQKHAE
EEEECCCCCCCCEECCHHHHCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHH
RYQIPLKLGRGGNLLEWREHGFKQNHFFASAAGRLIIDGIEALKSATWNFPSFSLEHVSQ
HEECCEEECCCCCCHHHHHCCCCCCCEEHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
TLLGEGKSIDNPYQRMAEIDRRFAEDKPALARYNLKDCELVTQIFAKTDLLTFLLERATV
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TGLAADRSGGSVAAFSNLYMPRMHRLGFVAPNLGEQPEEHSPGGFVMDSQPGLYDSVLVL
HHEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHH
DYKSLYPSIIRTFLIDPVGLVEGMQHPGDADSVPGFRHARFSRSKHCLPAIVEQIWQGRE
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCHH
AAKRQQNKALSQALKIIMNALYGVLGSSGCRFFDPRLASSITLRGHEIMRQTRELIEAQG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHEEECHHHHHHHHHHHHHHCC
YQVIYGDTDSTFVWLKQPHDEQQAAQIGRTLVEQVNQWWQQHLQQQYGLENALELEFETH
CEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEHHH
YSRFLMPTIRGAEQGSKKRYAGLIAKPDGSEEMVYKGLETVRTDWTPLAQQFQQQLYQRI
HHHHHHHHHCCCCCCCCHHHCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FKRQPYQDFVREYVSQTLNGDFDDRLVYRKRLRRKLDEYQRNVPPHVRAARIADDYNRQQ
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
GRPLQYQNGGWISYVMTVAGPEPLETQQSAIDYPHYLERQLEPVADAILPFLHDDFTTLV
CCCCEECCCCEEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
TGQMGLF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]