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The map label for this gene is ksgA

Identifier: 157368969

GI number: 157368969

Start: 801074

End: 801892

Strand: Reverse

Name: ksgA

Synonym: Spro_0724

Alternate gene names: 157368969

Gene position: 801892-801074 (Counterclockwise)

Preceding gene: 157368970

Following gene: 157368968

Centisome position: 14.72

GC content: 56.29

Gene sequence:

>819_bases
ATGAATAATAAAGTCCACCAAGGGCACTTTGCCCGCAAACGCTTTGGACAGAACTTTTTAACCGATCAGTTTGTCATCGA
CAGCATTGTCTCCGCCATTCACCCGATGCCGGGTGAAGCGGTAGTTGAAATCGGTCCCGGCCTGGGCGCACTGACCGAGC
CGGTCGGCGCACGCATGGATCGCATGACGGTGATTGAGCTGGACCGCGATTTGGCGGCCCGCCTGGCAAATCACCCACAG
CTGAAAGACAAACTGACCATTCACCAGCAAGATGCCATGACGGTGAACTTTGCCGAAATGGCCGAGCAGGCTGGCCAACC
GCTGCGCGTGTTTGGTAACCTGCCGTATAACATTTCGACGCCGCTGATGTTCCACCTTTTCAGCTATACTCAGGCCATTC
GCGACATGCACTTTATGTTGCAGAAAGAAGTGGTTAACCGCCTGGTTGCCGGCCCGAACAGCAAGGCCTACGGGCGTTTG
ACCGTAATGGCGCAGTACTACTGCAACGTTATTCCGGTGCTGGAAGTGCCACCGACCTCCTTCACCCCGGCACCGAAAGT
GGATTCCGCCGTGGTACGTCTGGTGCCACACCTGGTGAACCCGAACCCGGTAGGCGACGTGCGGATGCTGAGCCGTATTA
CCACTCAGGCGTTCAACCAACGCCGGAAAACCGTCCGTAACAGCCTGGGCGACCTGTTCACCCCAGAGCAACTGACGGAG
TTGGGTATCGATCCGATACTGAGAGCAGAGAATATTTCTGTGGCGCAATACTGTAAGCTGGCCAACTGGCTTTCAGCCAA
TCCCGCGCCGCAGCAATAA

Upstream 100 bases:

>100_bases
GACCACGGTACCGCTTTGGAACTGGCCGGCACCGGCACCGCCGATGCAGGCAGTTTTAAAACGGCCTTGAATCTCGCCAT
TAAAATGATAATTAATTGTA

Downstream 100 bases:

>100_bases
GGAGTAGTGGTCATGATTGATTCGCCCCGCGTATGTATTCAGGTACAAAGTGTCTATGTGGAATCACAGTCGATACCTGA
GGAAGAACGTTACGTCTTCG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNNKVHQGHFARKRFGQNFLTDQFVIDSIVSAIHPMPGEAVVEIGPGLGALTEPVGARMDRMTVIELDRDLAARLANHPQ
LKDKLTIHQQDAMTVNFAEMAEQAGQPLRVFGNLPYNISTPLMFHLFSYTQAIRDMHFMLQKEVVNRLVAGPNSKAYGRL
TVMAQYYCNVIPVLEVPPTSFTPAPKVDSAVVRLVPHLVNPNPVGDVRMLSRITTQAFNQRRKTVRNSLGDLFTPEQLTE
LGIDPILRAENISVAQYCKLANWLSANPAPQQ

Sequences:

>Translated_272_residues
MNNKVHQGHFARKRFGQNFLTDQFVIDSIVSAIHPMPGEAVVEIGPGLGALTEPVGARMDRMTVIELDRDLAARLANHPQ
LKDKLTIHQQDAMTVNFAEMAEQAGQPLRVFGNLPYNISTPLMFHLFSYTQAIRDMHFMLQKEVVNRLVAGPNSKAYGRL
TVMAQYYCNVIPVLEVPPTSFTPAPKVDSAVVRLVPHLVNPNPVGDVRMLSRITTQAFNQRRKTVRNSLGDLFTPEQLTE
LGIDPILRAENISVAQYCKLANWLSANPAPQQ
>Mature_272_residues
MNNKVHQGHFARKRFGQNFLTDQFVIDSIVSAIHPMPGEAVVEIGPGLGALTEPVGARMDRMTVIELDRDLAARLANHPQ
LKDKLTIHQQDAMTVNFAEMAEQAGQPLRVFGNLPYNISTPLMFHLFSYTQAIRDMHFMLQKEVVNRLVAGPNSKAYGRL
TVMAQYYCNVIPVLEVPPTSFTPAPKVDSAVVRLVPHLVNPNPVGDVRMLSRITTQAFNQRRKTVRNSLGDLFTPEQLTE
LGIDPILRAENISVAQYCKLANWLSANPAPQQ

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=282, Percent_Identity=31.9148936170213, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI7657198, Length=213, Percent_Identity=30.5164319248826, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786236, Length=272, Percent_Identity=80.5147058823529, Blast_Score=454, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI25141369, Length=286, Percent_Identity=27.6223776223776, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25146882, Length=192, Percent_Identity=33.8541666666667, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324989, Length=214, Percent_Identity=29.9065420560748, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357273, Length=279, Percent_Identity=29.7491039426523, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21358017, Length=229, Percent_Identity=32.7510917030568, Blast_Score=104, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_SERP5 (A8G9P0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476958.1
- ProteinModelPortal:   A8G9P0
- SMR:   A8G9P0
- STRING:   A8G9P0
- GeneID:   5605068
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0724
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   SPRO399741:SPRO_0724-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30335; Mature: 30335

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 18-18 BINDING 20-20 BINDING 45-45 BINDING 66-66 BINDING 91-91 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKVHQGHFARKRFGQNFLTDQFVIDSIVSAIHPMPGEAVVEIGPGLGALTEPVGARMD
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHH
RMTVIELDRDLAARLANHPQLKDKLTIHQQDAMTVNFAEMAEQAGQPLRVFGNLPYNIST
CCHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCEEEHHHHHHHCCCCEEEECCCCCCCCH
PLMFHLFSYTQAIRDMHFMLQKEVVNRLVAGPNSKAYGRLTVMAQYYCNVIPVLEVPPTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEEECCCCC
FTPAPKVDSAVVRLVPHLVNPNPVGDVRMLSRITTQAFNQRRKTVRNSLGDLFTPEQLTE
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LGIDPILRAENISVAQYCKLANWLSANPAPQQ
CCCCHHHCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNNKVHQGHFARKRFGQNFLTDQFVIDSIVSAIHPMPGEAVVEIGPGLGALTEPVGARMD
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHH
RMTVIELDRDLAARLANHPQLKDKLTIHQQDAMTVNFAEMAEQAGQPLRVFGNLPYNIST
CCHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCEEEHHHHHHHCCCCEEEECCCCCCCCH
PLMFHLFSYTQAIRDMHFMLQKEVVNRLVAGPNSKAYGRLTVMAQYYCNVIPVLEVPPTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEEECCCCC
FTPAPKVDSAVVRLVPHLVNPNPVGDVRMLSRITTQAFNQRRKTVRNSLGDLFTPEQLTE
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LGIDPILRAENISVAQYCKLANWLSANPAPQQ
CCCCHHHCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA