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The map label for this gene is rob [H]

Identifier: 157368924

GI number: 157368924

Start: 746674

End: 747543

Strand: Reverse

Name: rob [H]

Synonym: Spro_0679

Alternate gene names: 157368924

Gene position: 747543-746674 (Counterclockwise)

Preceding gene: 157368926

Following gene: 157368922

Centisome position: 13.72

GC content: 54.25

Gene sequence:

>870_bases
ATGGATCAAGCCGGTATTATTCGTGATCTGCTTAGCTGGCTGGAAAGCCATTTGGACCAACCTTTGTCACTGGACAATGT
GGCGGCCAAAGCAGGTTACTCTAAATGGCATTTACAACGGATGTTTAAAGATATTACCGGTAACGCCATCGGGGCTTATA
TCCGGGCAAGGAGATTATCCAAGGCTGCGGTAGCGCTGCGTTTAACCAGCCGACCTATTCTGGATATAGCCTTACAGTAT
CGCTTCGACTCGCAGCAGACATTTACCCGCGCATTTAAAAAACAGTTTGCGCAAACGCCGGCACTGTATCGCCGTGCAGA
AGACTGGAACTCATTCGGCATTTGTCCGCCAATCCGCCTGGGCGCCTTTACTCTGCCGCAACCCGAGTTTGTCACGATGC
CGGAACAGCACTTGATTGGCCTGACGCAGAGCTATTCCTGTACGCTGGAGCAAATTTCCAACTATCGTACCGAGCTGCGT
TCACAGTTCTGGCGCCAGTATCTGGGCGAAGCAGAGTCGCTGCCGCCGGTGTTATACGGGCTGCACCATTCACGTCCGAG
TCAGGAAAAGGATGACGAGCAGGAAGTGCTGTACACCACGGCGCTGGAACCGCATCACGTACCGGCGAAAATTCAGGCCG
GACAGCCGCTGGTGTTGCAAGGTGGCGAGTATGCGATGTTCAGCTATGAAGGCCCGGCTTCTGGCCTGCAGGACTTTATT
CTGACCGTTTATGGCACCTGCCTGCCGGCACTCAAACTCACGCGCCGTAAAGGGCACGATATTGAGCGCTTTTATCCCAA
GGGTGACCGCCGGCCACCCCAACCGCCAACCGAGATCAACTGCGACTATTTGATCCCGATCCGCCGTTAA

Upstream 100 bases:

>100_bases
TGCTACGCAAAATGGCTCATGGATTGGTCTGATAGCGCAAGCACCAGCGTAATGAATAAACAGTTCGGGTTCCTACCCTA
AAGCTCTACGAGGAAGATCT

Downstream 100 bases:

>100_bases
CGCTGCAGTTCATCCAGCGCGGGCGTGTCCAGATGCGTGACATCGCCCGCCGTTTCGACGATCCAGCCAGAAGCCAACCA
GGGACTCTGCTGATGATCGA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDQAGIIRDLLSWLESHLDQPLSLDNVAAKAGYSKWHLQRMFKDITGNAIGAYIRARRLSKAAVALRLTSRPILDIALQY
RFDSQQTFTRAFKKQFAQTPALYRRAEDWNSFGICPPIRLGAFTLPQPEFVTMPEQHLIGLTQSYSCTLEQISNYRTELR
SQFWRQYLGEAESLPPVLYGLHHSRPSQEKDDEQEVLYTTALEPHHVPAKIQAGQPLVLQGGEYAMFSYEGPASGLQDFI
LTVYGTCLPALKLTRRKGHDIERFYPKGDRRPPQPPTEINCDYLIPIRR

Sequences:

>Translated_289_residues
MDQAGIIRDLLSWLESHLDQPLSLDNVAAKAGYSKWHLQRMFKDITGNAIGAYIRARRLSKAAVALRLTSRPILDIALQY
RFDSQQTFTRAFKKQFAQTPALYRRAEDWNSFGICPPIRLGAFTLPQPEFVTMPEQHLIGLTQSYSCTLEQISNYRTELR
SQFWRQYLGEAESLPPVLYGLHHSRPSQEKDDEQEVLYTTALEPHHVPAKIQAGQPLVLQGGEYAMFSYEGPASGLQDFI
LTVYGTCLPALKLTRRKGHDIERFYPKGDRRPPQPPTEINCDYLIPIRR
>Mature_289_residues
MDQAGIIRDLLSWLESHLDQPLSLDNVAAKAGYSKWHLQRMFKDITGNAIGAYIRARRLSKAAVALRLTSRPILDIALQY
RFDSQQTFTRAFKKQFAQTPALYRRAEDWNSFGICPPIRLGAFTLPQPEFVTMPEQHLIGLTQSYSCTLEQISNYRTELR
SQFWRQYLGEAESLPPVLYGLHHSRPSQEKDDEQEVLYTTALEPHHVPAKIQAGQPLVLQGGEYAMFSYEGPASGLQDFI
LTVYGTCLPALKLTRRKGHDIERFYPKGDRRPPQPPTEINCDYLIPIRR

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=289, Percent_Identity=71.6262975778547, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1790497, Length=104, Percent_Identity=55.7692307692308, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI87081928, Length=101, Percent_Identity=49.5049504950495, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33079; Mature: 33079

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQAGIIRDLLSWLESHLDQPLSLDNVAAKAGYSKWHLQRMFKDITGNAIGAYIRARRLS
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
KAAVALRLTSRPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRAEDWNSFGICPPIRL
HHHHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHC
GAFTLPQPEFVTMPEQHLIGLTQSYSCTLEQISNYRTELRSQFWRQYLGEAESLPPVLYG
CEEECCCCCEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LHHSRPSQEKDDEQEVLYTTALEPHHVPAKIQAGQPLVLQGGEYAMFSYEGPASGLQDFI
HHCCCCCCCCCCHHHEEEEECCCCCCCCCEECCCCCEEEECCCEEEEECCCCHHHHHHHH
LTVYGTCLPALKLTRRKGHDIERFYPKGDRRPPQPPTEINCDYLIPIRR
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MDQAGIIRDLLSWLESHLDQPLSLDNVAAKAGYSKWHLQRMFKDITGNAIGAYIRARRLS
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
KAAVALRLTSRPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRAEDWNSFGICPPIRL
HHHHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHC
GAFTLPQPEFVTMPEQHLIGLTQSYSCTLEQISNYRTELRSQFWRQYLGEAESLPPVLYG
CEEECCCCCEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LHHSRPSQEKDDEQEVLYTTALEPHHVPAKIQAGQPLVLQGGEYAMFSYEGPASGLQDFI
HHCCCCCCCCCCHHHEEEEECCCCCCCCCEECCCCCEEEECCCEEEEECCCCHHHHHHHH
LTVYGTCLPALKLTRRKGHDIERFYPKGDRRPPQPPTEINCDYLIPIRR
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]