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The map label for this gene is yfiN [H]

Identifier: 157368918

GI number: 157368918

Start: 740443

End: 741690

Strand: Reverse

Name: yfiN [H]

Synonym: Spro_0673

Alternate gene names: 157368918

Gene position: 741690-740443 (Counterclockwise)

Preceding gene: 157368919

Following gene: 157368917

Centisome position: 13.61

GC content: 58.89

Gene sequence:

>1248_bases
ATGAAGTGGTTGCGCAAGAACCGATCGGGGCAGTCACGCCCGACGCTGGGCCGGGTGCTGCAACGGGTGCATCTTGGGCT
GGCGCTGATTGCCGTCGGCACCGCCGGGATTTTCCTGACGCTGGTCGCGCTGTTTGCGCTGCGCGCTTATGCCAACCATA
ACCTGCACCTGATTGCACGCTCCATCAGCTATACCGTCGAAGCTGCCGTGGTGTTTGAAGACCGCACCGCCGCAAGTGAG
GCGCTGGTATTGATCGCCTCCAAAGAGGAGGTATCGGAAGCCAGAATTCTGAACAACAGCGGCAAAGTGCTCGCCACCTG
GCGTCACCCCGAAGATGGCCCGCTGCACGGTATGGAACAAATCGTGGCCCGCTGGGCGCTGCCGGAACCGGTGATCCTGC
CGATTACTCATGAAGGCAAAGAGGTCGGCAAAGTTTGGCTGCTGGGCCACGGCGGCAGCCTGCTGCGCTTTCTGTTGCGC
GGCATGATCGGCATGATCGCCTGCCTGGTGCTCAGTACCCTGTGTGCGCTGGTGCTTTCCCGACGCATGCTGGTGGGCAT
TGTCCGTTCTCTGGATGACATCGCCAATGTCGCGCATGCGGTACGTCGCGATCGCGCCTTTGGCCTGCGGGTGCCGTCGG
CGCCGATTGCCGAACTGCATGAGCTGAGCAGCGACTTTAATGGCCTGCTTGACGAGCTGGAAGCCTGGCAGGCACACCTG
AAACAGGAAAATGACTCACTGACTCACCGGGCCACCCACGACAGCCTGACCGGCTTGCCGAATCGCGCCTTCTTTGAGGG
CCGTCTGAGCCGTGCGCTGGGGGACATTAAACCCAGCGAAAAACTGGCGGTGCTGTTTATCGACGGTGACCGTTTCAAGG
AAGTTAACGACAGTTACGGCCATGCCGCAGGCGATGTGGTGTTGACTACCATTGCCGGGCGCATTCGCGCGCAGCTGCGT
GAAAGCGATCTGGTGGCACGTTTGGGAGGTGATGAGTTCGCCGTATTGTTGGCACCGGTCCACACTACGGAAGATGTCCT
GCAGATTGCCGATAACATTATCGACTGCATGACCCAACCGGTGATTTTACCGAACGATGAGGCGGTGATTTCCTCTCTGA
GTATCGGTATTGCACTTTACCCAGAGCATGCGGTAACGCCTCAGGGACTCTTGCACGAAGCTGACGATGCGATGTACCAG
GCAAAGCATCGTTATAACGGAGGCTGGCGGCTGGCAATACAAAAATAA

Upstream 100 bases:

>100_bases
ACGAGGCCAGCTTTAAAGTGAACCTAGATGCACTGGCGCGCAGCGGCGTACGCGTTCACCCCAACGTGCTACAACTGGCA
CGCAAACCGGTGCCGCCGCT

Downstream 100 bases:

>100_bases
GGCCCCGGCTAAAAAATAAAACTAGGGATTTTGATTATGATACAGCAAACACTAAAAAACCGTTTTTCACTGCTGGCGAT
GATGTTTATCGCCCTGCTGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKWLRKNRSGQSRPTLGRVLQRVHLGLALIAVGTAGIFLTLVALFALRAYANHNLHLIARSISYTVEAAVVFEDRTAASE
ALVLIASKEEVSEARILNNSGKVLATWRHPEDGPLHGMEQIVARWALPEPVILPITHEGKEVGKVWLLGHGGSLLRFLLR
GMIGMIACLVLSTLCALVLSRRMLVGIVRSLDDIANVAHAVRRDRAFGLRVPSAPIAELHELSSDFNGLLDELEAWQAHL
KQENDSLTHRATHDSLTGLPNRAFFEGRLSRALGDIKPSEKLAVLFIDGDRFKEVNDSYGHAAGDVVLTTIAGRIRAQLR
ESDLVARLGGDEFAVLLAPVHTTEDVLQIADNIIDCMTQPVILPNDEAVISSLSIGIALYPEHAVTPQGLLHEADDAMYQ
AKHRYNGGWRLAIQK

Sequences:

>Translated_415_residues
MKWLRKNRSGQSRPTLGRVLQRVHLGLALIAVGTAGIFLTLVALFALRAYANHNLHLIARSISYTVEAAVVFEDRTAASE
ALVLIASKEEVSEARILNNSGKVLATWRHPEDGPLHGMEQIVARWALPEPVILPITHEGKEVGKVWLLGHGGSLLRFLLR
GMIGMIACLVLSTLCALVLSRRMLVGIVRSLDDIANVAHAVRRDRAFGLRVPSAPIAELHELSSDFNGLLDELEAWQAHL
KQENDSLTHRATHDSLTGLPNRAFFEGRLSRALGDIKPSEKLAVLFIDGDRFKEVNDSYGHAAGDVVLTTIAGRIRAQLR
ESDLVARLGGDEFAVLLAPVHTTEDVLQIADNIIDCMTQPVILPNDEAVISSLSIGIALYPEHAVTPQGLLHEADDAMYQ
AKHRYNGGWRLAIQK
>Mature_415_residues
MKWLRKNRSGQSRPTLGRVLQRVHLGLALIAVGTAGIFLTLVALFALRAYANHNLHLIARSISYTVEAAVVFEDRTAASE
ALVLIASKEEVSEARILNNSGKVLATWRHPEDGPLHGMEQIVARWALPEPVILPITHEGKEVGKVWLLGHGGSLLRFLLR
GMIGMIACLVLSTLCALVLSRRMLVGIVRSLDDIANVAHAVRRDRAFGLRVPSAPIAELHELSSDFNGLLDELEAWQAHL
KQENDSLTHRATHDSLTGLPNRAFFEGRLSRALGDIKPSEKLAVLFIDGDRFKEVNDSYGHAAGDVVLTTIAGRIRAQLR
ESDLVARLGGDEFAVLLAPVHTTEDVLQIADNIIDCMTQPVILPNDEAVISSLSIGIALYPEHAVTPQGLLHEADDAMYQ
AKHRYNGGWRLAIQK

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=403, Percent_Identity=36.2282878411911, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1788381, Length=163, Percent_Identity=40.4907975460123, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787541, Length=154, Percent_Identity=38.3116883116883, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=36.0759493670886, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=36.144578313253, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=34.1614906832298, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081977, Length=186, Percent_Identity=32.7956989247312, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=30.1204819277108, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787816, Length=189, Percent_Identity=30.1587301587302, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=34.3558282208589, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=32.7044025157233, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=162, Percent_Identity=32.7160493827161, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081921, Length=200, Percent_Identity=27.5, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45491; Mature: 45491

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWLRKNRSGQSRPTLGRVLQRVHLGLALIAVGTAGIFLTLVALFALRAYANHNLHLIAR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
SISYTVEAAVVFEDRTAASEALVLIASKEEVSEARILNNSGKVLATWRHPEDGPLHGMEQ
HHHHEEEEEEEEECCCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHH
IVARWALPEPVILPITHEGKEVGKVWLLGHGGSLLRFLLRGMIGMIACLVLSTLCALVLS
HHHHHCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRMLVGIVRSLDDIANVAHAVRRDRAFGLRVPSAPIAELHELSSDFNGLLDELEAWQAHL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQENDSLTHRATHDSLTGLPNRAFFEGRLSRALGDIKPSEKLAVLFIDGDRFKEVNDSYG
HCCCCCHHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHCCCCCC
HAAGDVVLTTIAGRIRAQLRESDLVARLGGDEFAVLLAPVHTTEDVLQIADNIIDCMTQP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCC
VILPNDEAVISSLSIGIALYPEHAVTPQGLLHEADDAMYQAKHRYNGGWRLAIQK
EECCCCHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure
MKWLRKNRSGQSRPTLGRVLQRVHLGLALIAVGTAGIFLTLVALFALRAYANHNLHLIAR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
SISYTVEAAVVFEDRTAASEALVLIASKEEVSEARILNNSGKVLATWRHPEDGPLHGMEQ
HHHHEEEEEEEEECCCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHH
IVARWALPEPVILPITHEGKEVGKVWLLGHGGSLLRFLLRGMIGMIACLVLSTLCALVLS
HHHHHCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRMLVGIVRSLDDIANVAHAVRRDRAFGLRVPSAPIAELHELSSDFNGLLDELEAWQAHL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQENDSLTHRATHDSLTGLPNRAFFEGRLSRALGDIKPSEKLAVLFIDGDRFKEVNDSYG
HCCCCCHHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHCCCCCC
HAAGDVVLTTIAGRIRAQLRESDLVARLGGDEFAVLLAPVHTTEDVLQIADNIIDCMTQP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCC
VILPNDEAVISSLSIGIALYPEHAVTPQGLLHEADDAMYQAKHRYNGGWRLAIQK
EECCCCHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]