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The map label for this gene is yjeA

Identifier: 157368667

GI number: 157368667

Start: 467167

End: 468144

Strand: Direct

Name: yjeA

Synonym: Spro_0420

Alternate gene names: 157368667

Gene position: 467167-468144 (Clockwise)

Preceding gene: 157368662

Following gene: 157368671

Centisome position: 8.57

GC content: 60.02

Gene sequence:

>978_bases
ATGAGCGAAACGGCAAGCTGGCAGCCAAGTGCACCCATCGCCAATTTGTTGAAGCGCGCAGCGATCCTGACTGAGATCCG
GCGTTTCTTCGCCGATCGTGGCGTATTGGAAGTTGAGACGCCAACCATGAGCCAGGCGACGGTCACCGATATCCACCTGT
TCCCGTTCCAGACGCGTTTCGTCGGGCCCGGCGCGGCGGATGGCCTGACGCTGTACATGATGACCAGCCCGGAATACCAC
ATGAAGCGCTTGCTGGCGGCGGGCAGCGGTCCGATCTATCAGATGGGGCGCAGCTTCCGTAATGAAGAAGCCGGCCGTCA
CCACAACCCGGAATTCACCATGCTGGAGTGGTACCGCCCGCATTACGACATGTACCGTCTGATGAATGAGGTGGACGATC
TGCTGCAGCAGGTGCTGGATTGCGACAGCGCGGAAACCCTGTCCTATCAGCAGGCTTTCCTGCGCCATCTGGATATCGAT
CCGCTGTCAGCGGAGAAGGCACAACTGCGTGAAGCGGCGGCCAAGCTGGATCTGAGCAACATTGCCGATACGGAAGAAGA
TCGCGATACGCTGTTGCAACTGCTGTTTACCGTCGGCGTGGAGCCGCACATTGGCCGTGACAAACCGGCGTTCGTTTATC
ACTTCCCGGCCAGCCAGGCGTCGCTGGCGGAGATCAGTACCGAGGATCACCGGGTGGCGGAGCGTTTCGAGGTTTACTTC
AAAGGCATTGAGCTGGCGAATGGCTTCCGTGAACTGACGGACAGCCGCGAACAAGGGCAGCGTTTTGCCCAGGACAACCG
CAAGCGTGCGGAACGTGGCCTGCCGCAGCACCCGATTGATAACAACCTGCTGGATGCTTTGCAGCACGGCATGCCTGAAT
GCTCTGGCGTGGCGCTGGGGGTTGACCGTTTGGTGATGCTGGCGCTGGGTGCCGAGAGCCTGAGTGAAGTGCTGGCGTTC
CCGGTCGATCGCGCATAA

Upstream 100 bases:

>100_bases
AGGTAATGTCGCGGTTAAAAGTCCAGGGCCTGTTAAGAATTTGTTTGCATGCCGGGATACGAGTAGACTGCATCCCCTGT
TTGATTTCGGAGTAATTACC

Downstream 100 bases:

>100_bases
CTATATATCCGTCGTCTTTCAAGCCGCAGCGTTGTTACCTGCACTCGCTCACCCCAGTTACTTACTGAAGTAAGCGCCTG
GGGATGAGCGAGCTGGGCGC

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSETASWQPSAPIANLLKRAAILTEIRRFFADRGVLEVETPTMSQATVTDIHLFPFQTRFVGPGAADGLTLYMMTSPEYH
MKRLLAAGSGPIYQMGRSFRNEEAGRHHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCDSAETLSYQQAFLRHLDID
PLSAEKAQLREAAAKLDLSNIADTEEDRDTLLQLLFTVGVEPHIGRDKPAFVYHFPASQASLAEISTEDHRVAERFEVYF
KGIELANGFRELTDSREQGQRFAQDNRKRAERGLPQHPIDNNLLDALQHGMPECSGVALGVDRLVMLALGAESLSEVLAF
PVDRA

Sequences:

>Translated_325_residues
MSETASWQPSAPIANLLKRAAILTEIRRFFADRGVLEVETPTMSQATVTDIHLFPFQTRFVGPGAADGLTLYMMTSPEYH
MKRLLAAGSGPIYQMGRSFRNEEAGRHHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCDSAETLSYQQAFLRHLDID
PLSAEKAQLREAAAKLDLSNIADTEEDRDTLLQLLFTVGVEPHIGRDKPAFVYHFPASQASLAEISTEDHRVAERFEVYF
KGIELANGFRELTDSREQGQRFAQDNRKRAERGLPQHPIDNNLLDALQHGMPECSGVALGVDRLVMLALGAESLSEVLAF
PVDRA
>Mature_324_residues
SETASWQPSAPIANLLKRAAILTEIRRFFADRGVLEVETPTMSQATVTDIHLFPFQTRFVGPGAADGLTLYMMTSPEYHM
KRLLAAGSGPIYQMGRSFRNEEAGRHHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCDSAETLSYQQAFLRHLDIDP
LSAEKAQLREAAAKLDLSNIADTEEDRDTLLQLLFTVGVEPHIGRDKPAFVYHFPASQASLAEISTEDHRVAERFEVYFK
GIELANGFRELTDSREQGQRFAQDNRKRAERGLPQHPIDNNLLDALQHGMPECSGVALGVDRLVMLALGAESLSEVLAFP
VDRA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI194272210, Length=335, Percent_Identity=28.3582089552239, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI5031815, Length=335, Percent_Identity=28.3582089552239, Blast_Score=124, Evalue=8e-29,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=83.6923076923077, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1790571, Length=325, Percent_Identity=29.2307692307692, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789256, Length=329, Percent_Identity=29.7872340425532, Blast_Score=138, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71994340, Length=342, Percent_Identity=28.9473684210526, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17535925, Length=342, Percent_Identity=28.9473684210526, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17535927, Length=342, Percent_Identity=28.9473684210526, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320242, Length=335, Percent_Identity=27.7611940298507, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324256, Length=347, Percent_Identity=27.6657060518732, Blast_Score=106, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24640849, Length=333, Percent_Identity=27.3273273273273, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24640851, Length=333, Percent_Identity=27.6276276276276, Blast_Score=114, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YJEA_SERP5 (A8G8T8)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476656.1
- ProteinModelPortal:   A8G8T8
- SMR:   A8G8T8
- STRING:   A8G8T8
- GeneID:   5602500
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0420
- eggNOG:   COG2269
- HOGENOM:   HBG631383
- OMA:   MPEASGI
- ProtClustDB:   PRK09350
- BioCyc:   SPRO399741:SPRO_0420-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00174
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149
- PANTHER:   PTHR22594
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00462

Pfam domain/function: PF00152 tRNA-synt_2

EC number: 6.1.1.6

Molecular weight: Translated: 36680; Mature: 36549

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETASWQPSAPIANLLKRAAILTEIRRFFADRGVLEVETPTMSQATVTDIHLFPFQTRF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCHHEE
VGPGAADGLTLYMMTSPEYHMKRLLAAGSGPIYQMGRSFRNEEAGRHHNPEFTMLEWYRP
CCCCCCCCEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEHHHCC
HYDMYRLMNEVDDLLQQVLDCDSAETLSYQQAFLRHLDIDPLSAEKAQLREAAAKLDLSN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHH
IADTEEDRDTLLQLLFTVGVEPHIGRDKPAFVYHFPASQASLAEISTEDHRVAERFEVYF
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHH
KGIELANGFRELTDSREQGQRFAQDNRKRAERGLPQHPIDNNLLDALQHGMPECSGVALG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
VDRLVMLALGAESLSEVLAFPVDRA
HHHHHHHHHCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SETASWQPSAPIANLLKRAAILTEIRRFFADRGVLEVETPTMSQATVTDIHLFPFQTRF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCHHEE
VGPGAADGLTLYMMTSPEYHMKRLLAAGSGPIYQMGRSFRNEEAGRHHNPEFTMLEWYRP
CCCCCCCCEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEHHHCC
HYDMYRLMNEVDDLLQQVLDCDSAETLSYQQAFLRHLDIDPLSAEKAQLREAAAKLDLSN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHH
IADTEEDRDTLLQLLFTVGVEPHIGRDKPAFVYHFPASQASLAEISTEDHRVAERFEVYF
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHH
KGIELANGFRELTDSREQGQRFAQDNRKRAERGLPQHPIDNNLLDALQHGMPECSGVALG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
VDRLVMLALGAESLSEVLAFPVDRA
HHHHHHHHHCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA