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The map label for this gene is istB [H]

Identifier: 157368606

GI number: 157368606

Start: 408555

End: 409343

Strand: Direct

Name: istB [H]

Synonym: Spro_0357

Alternate gene names: 157368606

Gene position: 408555-409343 (Clockwise)

Preceding gene: 157368605

Following gene: 157368611

Centisome position: 7.5

GC content: 53.23

Gene sequence:

>789_bases
ATGAGCCTTCAGCATCAACGTATTAGCGAGCTCTGTGCCGACTTCAAGCTTGAACGTATTGCCAGTGAGTGGCCCGCACT
CGCCCCAAAAACACTGGAGGATACCGGGACGTTCGGCGACTTTTTGGAACAGCTTCTGCAACTGGAGACGAACAACCGTA
ACGAACGTCGCCGGCAGACGCTTTTGCGGTTATCAGGACTGCCAGCGGTTAAAACGCTGGAGCAGTTCGACTTCAAGTTC
GCCAGCGGTGCGCCGCGAGCTCAGCTTCAGGAACTCGCCGGACTGGCCTTCATAGAGCGGCACGAGAACATCGTGCTACT
GGGTCCTTCGGGCGTAGGCAAGAGCCATCTAGCCTGTGCGTTGGCACATAAAGTCGCGATGTCAGGTCAGAGTGTTCGCT
TTATAACGGCAGCAGACATGATGTTGCAGCTGGTCGCAGCTCACCGACAGGGAGAGCTCAAGGGATATTTAGGGCGCTGT
GTCAATAAGCCGCGACTGCTGGTGATTGATGAGGTGGGGTATCTGCCGTTCGGCAAGGAAGAGGCTAATTTATTCTTCCA
GGTTGTCGCCCGGCGCTATGAGACCGGAAGTCTGATCCTCACGAGTAATCTGCCGTTTACTCAGTGGTCAGGTACGTTTG
GTGATGATGAAACACTGACGGCAGCGATGTTAGACCGACTGCTTCACCACGCACACATAGTCCAGATAAGTGGTCAAAGC
TATCGCCTGAAAGATAAACTGAAAAGTGGTCAGTTAAAGAAATCGAAGGTCCCTGAACCACAGGAATAA

Upstream 100 bases:

>100_bases
CAAGGACAGTACCCGTCGTCGCGGATAATCAAAGGGTTATACCAAGAGAGAGTTTCCAACATCCATTGTCTGTCTATCAG
TCGCTGCTGGAGGCGGGATT

Downstream 100 bases:

>100_bases
TCATTGGTGGGTGGGTCAGATTTACTTTGGCAGGGTGGGTCACAATTCAATCGGTATTGACAGATTAAACAGCTTTTTGT
CGTGGAGATCCCTGATTCGC

Product: transposase/IS protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSLQHQRISELCADFKLERIASEWPALAPKTLEDTGTFGDFLEQLLQLETNNRNERRRQTLLRLSGLPAVKTLEQFDFKF
ASGAPRAQLQELAGLAFIERHENIVLLGPSGVGKSHLACALAHKVAMSGQSVRFITAADMMLQLVAAHRQGELKGYLGRC
VNKPRLLVIDEVGYLPFGKEEANLFFQVVARRYETGSLILTSNLPFTQWSGTFGDDETLTAAMLDRLLHHAHIVQISGQS
YRLKDKLKSGQLKKSKVPEPQE

Sequences:

>Translated_262_residues
MSLQHQRISELCADFKLERIASEWPALAPKTLEDTGTFGDFLEQLLQLETNNRNERRRQTLLRLSGLPAVKTLEQFDFKF
ASGAPRAQLQELAGLAFIERHENIVLLGPSGVGKSHLACALAHKVAMSGQSVRFITAADMMLQLVAAHRQGELKGYLGRC
VNKPRLLVIDEVGYLPFGKEEANLFFQVVARRYETGSLILTSNLPFTQWSGTFGDDETLTAAMLDRLLHHAHIVQISGQS
YRLKDKLKSGQLKKSKVPEPQE
>Mature_261_residues
SLQHQRISELCADFKLERIASEWPALAPKTLEDTGTFGDFLEQLLQLETNNRNERRRQTLLRLSGLPAVKTLEQFDFKFA
SGAPRAQLQELAGLAFIERHENIVLLGPSGVGKSHLACALAHKVAMSGQSVRFITAADMMLQLVAAHRQGELKGYLGRCV
NKPRLLVIDEVGYLPFGKEEANLFFQVVARRYETGSLILTSNLPFTQWSGTFGDDETLTAAMLDRLLHHAHIVQISGQSY
RLKDKLKSGQLKKSKVPEPQE

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 29296; Mature: 29165

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQHQRISELCADFKLERIASEWPALAPKTLEDTGTFGDFLEQLLQLETNNRNERRRQT
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHH
LLRLSGLPAVKTLEQFDFKFASGAPRAQLQELAGLAFIERHENIVLLGPSGVGKSHLACA
HHHHCCCCHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
LAHKVAMSGQSVRFITAADMMLQLVAAHRQGELKGYLGRCVNKPRLLVIDEVGYLPFGKE
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCH
EANLFFQVVARRYETGSLILTSNLPFTQWSGTFGDDETLTAAMLDRLLHHAHIVQISGQS
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCC
YRLKDKLKSGQLKKSKVPEPQE
EEHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLQHQRISELCADFKLERIASEWPALAPKTLEDTGTFGDFLEQLLQLETNNRNERRRQT
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHH
LLRLSGLPAVKTLEQFDFKFASGAPRAQLQELAGLAFIERHENIVLLGPSGVGKSHLACA
HHHHCCCCHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
LAHKVAMSGQSVRFITAADMMLQLVAAHRQGELKGYLGRCVNKPRLLVIDEVGYLPFGKE
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCH
EANLFFQVVARRYETGSLILTSNLPFTQWSGTFGDDETLTAAMLDRLLHHAHIVQISGQS
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCC
YRLKDKLKSGQLKKSKVPEPQE
EEHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]