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The map label for this gene is asnB [H]

Identifier: 157368595

GI number: 157368595

Start: 397882

End: 399714

Strand: Direct

Name: asnB [H]

Synonym: Spro_0346

Alternate gene names: 157368595

Gene position: 397882-399714 (Clockwise)

Preceding gene: 157368594

Following gene: 157368596

Centisome position: 7.3

GC content: 55.05

Gene sequence:

>1833_bases
ATGTGTGGTATTGCAGGCGTCATCAACTTTCATCGCCAACCGGCGCAGCCCCCACTGTTAGCGTCGATGTTAGCCGCTCT
GACGCACCGTGGTCCCGATCAAGAGGGCACCTATCATCAAGGGCATGTCAGCATTGGCATGCGACGTTTGAAAATCATCG
CGCTGGATAACGGCGCTCAGCCCTGCTACAGCAATGATGGGCGATATGTATTGGTGTTTAACGGTGAGATCTATAATTAC
CGGGCTCTGCGCGCTGAGTTAATAGCGCAGGGATTTAGATTCGACACTGAATCCGACGCGGAAGTGATAGTTAACCTGTA
TCAACGCGACGGTGAAGCCTTTTATCAGCGTCTGGATGGCATGTTCGCTCTGGCCATTTACGACAGAATCAGCGATACGC
TGCTGCTGGCCCGCGATCCAGCCGGCAAGAAACCGCTGTTTTATGCCCTTCGCGACGGGGTAGTCAGCTTTTCGTCCGAG
TTGAACTCCTTACTGAAGGATCAAAGCATCCCACGCCGTATCGATCCTGTGGCGCTGGATTATTATCTGCGCTTTCGTGT
GGTGCCGGGCGATCGCTGCATTTTCCAGGGCATCAGTAAAGTGCCTGCCGGTGGCGGCGTCAGCTTCACGCTCGGGCAAG
AAAGCCGACAGCACTATTGGCGCGTGGCCTATCGGCCGACGGAGGATGACAAGCCGCTGGAACAGTGGGTTGACGAGATC
GACGAATATTTGAATCAGGCTATCGCCAAACGCTTGATGGCGGAAGTGCCGATCGGCACCATGCTAAGCGGTGGGCTTGA
TTCCAGCCTGATTACTGCCATCGCCTGCCGGCAGGGCCGGCGTGAGCTGAAAACCTTCGCCGTGGGTTTCAATGAATCCG
CCTTCAGTGAACTGGATTATTCCCGACGCCTGGCGAAAGATCTCGGCACCGAACATCATGATTACATTATTACACCACAG
GAGGCATTTGACGCAGCACATGGGTTGGTCAGCCACTTCGGCGAGCCTTTTGCGTTCCCCTCTTCGATAGCCAGTCACTT
TATGTATCGTCTGGCTCGTCAACATGTGACGGTGGTGCTGGGCGGCGATGGCGCCGACGAACTGTTTGGCGGCTATGCCC
GCTACTCGCTGGTGGCCAATTTCCCTCATTTACCCACCGAGCATAACCTGCCGCGCAAGGTAGACCTGTTCAACAAGACG
TGGCAGGCGGATGCATTCCCCGAATTTTATCAGGCGCTGTTGACCGATGGCGCAGGCGGCCAGTTGCGCCAACAGCTCTA
CAGCCGACAAATGGCGTTGCAGTTGGCCGAGGAAAATAGCGTGGCGCAAGTCAGAAGCTACAGCGATGAATTGACGCAGC
GTCCCGATCCTCTTTCCGCTGCTATGGAATATGATTTCAATCATTGGATGCAGGAAGCGCAGATGGTCAAGGTAGACATA
GCCTCGATGGCCAATTCGCTCGAGGTGCGCGTTCCGTTCCTGGACAAGCAGGTCGTCGGGCTTGGCAGCAGTTTGCCGGC
GCGGTTCAAACTGCACGCAGGCAAGGAGAAATATCTGTTGCACTGTCTGGCGAAACGCTACCTTCCCGACTATATCGTTA
ATCGCAAAAAACAGGAGTTGGCAGTGCCATTGGAGCAGTGGATGGTGTCGTCGATGAAAACGCGCATCATCGACACGCTG
ACCAGCGAGCAGGCGCTGTCGCGCGGGTATTTTAATCCTGACAGGCTGCGCCAGTTCGTCAGAGAATTCGACGCTGGCCA
CAGCTACGCGTTGTGGACAATGTATATGCTGGAAAAATGGCACCAGCAGTTTGTAGACAATCAGGAGTCGTGA

Upstream 100 bases:

>100_bases
GGACTGGTCGGGGATATCCGCATCCAGTGCGTCGACGCGCAGGAAATCGCCGGCAACGTCAGCAAACGCCGCTATTGAGC
AGTATCAACGAGGAAAATCT

Downstream 100 bases:

>100_bases
ATGACGGATCAAGGTAGGTACCTGTCAGGCGTGTTTTGTACGTTGCTGTATATTGTCATAGCCTCGACGCAGAGCGTGAT
GTTGAATATCTGGCTGTTCG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MCGIAGVINFHRQPAQPPLLASMLAALTHRGPDQEGTYHQGHVSIGMRRLKIIALDNGAQPCYSNDGRYVLVFNGEIYNY
RALRAELIAQGFRFDTESDAEVIVNLYQRDGEAFYQRLDGMFALAIYDRISDTLLLARDPAGKKPLFYALRDGVVSFSSE
LNSLLKDQSIPRRIDPVALDYYLRFRVVPGDRCIFQGISKVPAGGGVSFTLGQESRQHYWRVAYRPTEDDKPLEQWVDEI
DEYLNQAIAKRLMAEVPIGTMLSGGLDSSLITAIACRQGRRELKTFAVGFNESAFSELDYSRRLAKDLGTEHHDYIITPQ
EAFDAAHGLVSHFGEPFAFPSSIASHFMYRLARQHVTVVLGGDGADELFGGYARYSLVANFPHLPTEHNLPRKVDLFNKT
WQADAFPEFYQALLTDGAGGQLRQQLYSRQMALQLAEENSVAQVRSYSDELTQRPDPLSAAMEYDFNHWMQEAQMVKVDI
ASMANSLEVRVPFLDKQVVGLGSSLPARFKLHAGKEKYLLHCLAKRYLPDYIVNRKKQELAVPLEQWMVSSMKTRIIDTL
TSEQALSRGYFNPDRLRQFVREFDAGHSYALWTMYMLEKWHQQFVDNQES

Sequences:

>Translated_610_residues
MCGIAGVINFHRQPAQPPLLASMLAALTHRGPDQEGTYHQGHVSIGMRRLKIIALDNGAQPCYSNDGRYVLVFNGEIYNY
RALRAELIAQGFRFDTESDAEVIVNLYQRDGEAFYQRLDGMFALAIYDRISDTLLLARDPAGKKPLFYALRDGVVSFSSE
LNSLLKDQSIPRRIDPVALDYYLRFRVVPGDRCIFQGISKVPAGGGVSFTLGQESRQHYWRVAYRPTEDDKPLEQWVDEI
DEYLNQAIAKRLMAEVPIGTMLSGGLDSSLITAIACRQGRRELKTFAVGFNESAFSELDYSRRLAKDLGTEHHDYIITPQ
EAFDAAHGLVSHFGEPFAFPSSIASHFMYRLARQHVTVVLGGDGADELFGGYARYSLVANFPHLPTEHNLPRKVDLFNKT
WQADAFPEFYQALLTDGAGGQLRQQLYSRQMALQLAEENSVAQVRSYSDELTQRPDPLSAAMEYDFNHWMQEAQMVKVDI
ASMANSLEVRVPFLDKQVVGLGSSLPARFKLHAGKEKYLLHCLAKRYLPDYIVNRKKQELAVPLEQWMVSSMKTRIIDTL
TSEQALSRGYFNPDRLRQFVREFDAGHSYALWTMYMLEKWHQQFVDNQES
>Mature_610_residues
MCGIAGVINFHRQPAQPPLLASMLAALTHRGPDQEGTYHQGHVSIGMRRLKIIALDNGAQPCYSNDGRYVLVFNGEIYNY
RALRAELIAQGFRFDTESDAEVIVNLYQRDGEAFYQRLDGMFALAIYDRISDTLLLARDPAGKKPLFYALRDGVVSFSSE
LNSLLKDQSIPRRIDPVALDYYLRFRVVPGDRCIFQGISKVPAGGGVSFTLGQESRQHYWRVAYRPTEDDKPLEQWVDEI
DEYLNQAIAKRLMAEVPIGTMLSGGLDSSLITAIACRQGRRELKTFAVGFNESAFSELDYSRRLAKDLGTEHHDYIITPQ
EAFDAAHGLVSHFGEPFAFPSSIASHFMYRLARQHVTVVLGGDGADELFGGYARYSLVANFPHLPTEHNLPRKVDLFNKT
WQADAFPEFYQALLTDGAGGQLRQQLYSRQMALQLAEENSVAQVRSYSDELTQRPDPLSAAMEYDFNHWMQEAQMVKVDI
ASMANSLEVRVPFLDKQVVGLGSSLPARFKLHAGKEKYLLHCLAKRYLPDYIVNRKKQELAVPLEQWMVSSMKTRIIDTL
TSEQALSRGYFNPDRLRQFVREFDAGHSYALWTMYMLEKWHQQFVDNQES

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=399, Percent_Identity=28.3208020050125, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI168229252, Length=399, Percent_Identity=28.3208020050125, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI168229250, Length=399, Percent_Identity=28.3208020050125, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI296010848, Length=368, Percent_Identity=28.804347826087, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI296010852, Length=299, Percent_Identity=28.7625418060201, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI296010850, Length=299, Percent_Identity=28.7625418060201, Blast_Score=107, Evalue=4e-23,
Organism=Escherichia coli, GI1786889, Length=408, Percent_Identity=33.3333333333333, Blast_Score=182, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI25147557, Length=383, Percent_Identity=29.5039164490862, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71993933, Length=383, Percent_Identity=29.5039164490862, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17560178, Length=405, Percent_Identity=29.6296296296296, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147560, Length=313, Percent_Identity=28.7539936102236, Blast_Score=110, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6325403, Length=438, Percent_Identity=30.365296803653, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321563, Length=437, Percent_Identity=31.1212814645309, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI45553209, Length=395, Percent_Identity=31.3924050632911, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69261; Mature: 69261

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGVINFHRQPAQPPLLASMLAALTHRGPDQEGTYHQGHVSIGMRRLKIIALDNGAQ
CCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCHHHCCEEEEEEEEECCCCC
PCYSNDGRYVLVFNGEIYNYRALRAELIAQGFRFDTESDAEVIVNLYQRDGEAFYQRLDG
CCCCCCCCEEEEECCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCHHHHHHHHCC
MFALAIYDRISDTLLLARDPAGKKPLFYALRDGVVSFSSELNSLLKDQSIPRRIDPVALD
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YYLRFRVVPGDRCIFQGISKVPAGGGVSFTLGQESRQHYWRVAYRPTEDDKPLEQWVDEI
EEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCHHHCCEEEEEECCCCCCCHHHHHHHHH
DEYLNQAIAKRLMAEVPIGTMLSGGLDSSLITAIACRQGRRELKTFAVGFNESAFSELDY
HHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SRRLAKDLGTEHHDYIITPQEAFDAAHGLVSHFGEPFAFPSSIASHFMYRLARQHVTVVL
HHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEE
GGDGADELFGGYARYSLVANFPHLPTEHNLPRKVDLFNKTWQADAFPEFYQALLTDGAGG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHCCCCCCCCCHHHHHHHHCCCCCH
QLRQQLYSRQMALQLAEENSVAQVRSYSDELTQRPDPLSAAMEYDFNHWMQEAQMVKVDI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASMANSLEVRVPFLDKQVVGLGSSLPARFKLHAGKEKYLLHCLAKRYLPDYIVNRKKQEL
HHHHCCCEEEECCCCHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCHHHHHCCCHHHH
AVPLEQWMVSSMKTRIIDTLTSEQALSRGYFNPDRLRQFVREFDAGHSYALWTMYMLEKW
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
HQQFVDNQES
HHHHCCCCCC
>Mature Secondary Structure
MCGIAGVINFHRQPAQPPLLASMLAALTHRGPDQEGTYHQGHVSIGMRRLKIIALDNGAQ
CCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCHHHCCEEEEEEEEECCCCC
PCYSNDGRYVLVFNGEIYNYRALRAELIAQGFRFDTESDAEVIVNLYQRDGEAFYQRLDG
CCCCCCCCEEEEECCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCHHHHHHHHCC
MFALAIYDRISDTLLLARDPAGKKPLFYALRDGVVSFSSELNSLLKDQSIPRRIDPVALD
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YYLRFRVVPGDRCIFQGISKVPAGGGVSFTLGQESRQHYWRVAYRPTEDDKPLEQWVDEI
EEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCHHHCCEEEEEECCCCCCCHHHHHHHHH
DEYLNQAIAKRLMAEVPIGTMLSGGLDSSLITAIACRQGRRELKTFAVGFNESAFSELDY
HHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SRRLAKDLGTEHHDYIITPQEAFDAAHGLVSHFGEPFAFPSSIASHFMYRLARQHVTVVL
HHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEE
GGDGADELFGGYARYSLVANFPHLPTEHNLPRKVDLFNKTWQADAFPEFYQALLTDGAGG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHCCCCCCCCCHHHHHHHHCCCCCH
QLRQQLYSRQMALQLAEENSVAQVRSYSDELTQRPDPLSAAMEYDFNHWMQEAQMVKVDI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASMANSLEVRVPFLDKQVVGLGSSLPARFKLHAGKEKYLLHCLAKRYLPDYIVNRKKQEL
HHHHCCCEEEECCCCHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCHHHHHCCCHHHH
AVPLEQWMVSSMKTRIIDTLTSEQALSRGYFNPDRLRQFVREFDAGHSYALWTMYMLEKW
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
HQQFVDNQES
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]