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The map label for this gene is topB [H]

Identifier: 157368559

GI number: 157368559

Start: 364529

End: 366568

Strand: Direct

Name: topB [H]

Synonym: Spro_0310

Alternate gene names: 157368559

Gene position: 364529-366568 (Clockwise)

Preceding gene: 157368558

Following gene: 157368560

Centisome position: 6.69

GC content: 57.99

Gene sequence:

>2040_bases
ATGCAACTCTACCTGTGCGAAAAACCCAGTCAGGGTAAAGATATCGGCGCCATTCTCGGGGCGACGCAGCGAAAACAGGG
TTACCTGGCGGGCGCTGGCGTGATAGTCACCTGGGCTATAGGGCATCTGCTGGAACAGGCCCAGCCGGAGACCTACGGTG
AGCAGTTCGGCAGTCCCTGGCGGGAGTCCGTTCTTCCTATTGTCCCTACCCAGTGGAAAATGGCGGTAAAAAAAGACACT
GCCGACCAGTTCGCTGTTATCCGCGGGTTGCTCAAACAGGTTGATTCTGTGGTGATTGCGACCGATGCGGATCGCGAGGG
GGAAGTGATCGCGCGGGAGTTACTGGAACATTGCCACTTTACCGGCCCGGTCCAGCGGCTCTGGTTGTCAGCGCTGGACG
AGGCCAGTATTCGGCATGCCCTGGCGAACAAACTCCCTGGCGAGAAGACCGCGCCCCTGTATGAAGCTGGCCTGGCGCGA
TCACGTGCTGACTGGATGACGGGGATGAATTTAACCCGGCTTTATACGCTCAAAGCCCGTGAGCAGGGATACGGCGAGGT
ATTGTCGATCGGGCGGGTGCAGACCCCCACGCTGGCGCTGGTCGTGAACCGAGACCGCGACATTACCAACTTCATCTCCA
AACCGTACTGGCAAGTCAGGGCCACGCTGCAAAAAGGCGGGATCACTTTCCCGGCAAACTGGGTACCAGCCGCCAACTAC
TGTGATGAAGAGAAGCGCTGTATTCAACAGAATGTGGCGCAGGCCGTCGTGCAGCGGTGTCAGCAAACGGCTCATGCCGT
TGTGCTTGAGGTGGGGACCGAGCGCAAGAAAGAGTCCGCGCCGTTGGCATTTGATCTGGGCACCCTGCAGCAGGCTTGCT
CCCGCAAGTGGGGGATGAGCGCCAACCAGGTCTTGAGTATCGCACAGTCACTGTATGAGACGCACAAGGCCACCACATAT
CCCCGGACGGATTGCGGCTATCTGCCGACATCGATGCGGGAAGAGGTTGCCGATGTGCTGAGCGCTGTTACCCGCACGGA
CCCAACGCTCAACGAAAGCGTGGCCAGGCTCGACAGGGCTTTTGTTTCCCGTATCTGGAATGACAAAAAAATTACGGCGC
ACCACGGGATTATCCCAACCAAACAGCCGTTCGACCTGAGCAAGTTGTCAGCCGATGAGCTCAAGGTCTATCAGCTGATC
CGTCAGCATTACCTGGCACAGTACCTGTCCTTACATGAAGTAGATGTGACGGAAGCCACCTTCAATATCGGCGGGCAACT
GTTCCGCACCCGCGGCAAGGTCGAGGCTGTTGCTGGATGGAAAGTGTTGTTCCAGCATGACGTGAGCGAGGCGAAAGAGG
AACCGGCTGACACGGATGATGTCCGGCTGCCGGCGCTGGTTAAAGGGGAAACCGTCGCGGTAGCCGGCGCTGAGCTCAAA
ACGTTGCAGACGAAGCCCCCAGGACATTTCACCGACGGGACACTGATCGCCGCCATGAAAAATGCAGCGGCGTTCGTCAG
TGACCCGCAACTGAAGAAAGTGTTGAAAGAGAACGCAGGGCTGGGTACTGAAGCGACGCGCGCCGGTATCCTGGATACGC
TGTTTAAACGCGGGTACCTGGAGAAGAAGGGCAAATACCTACTCTCCACACCGATCGCCGGCGAACTGATTGATGCGTTA
CCGACTGCGCTGACCAACCCCGGGATGACGGCGCTGTGGGAGCAGGCGCTGGACGAGATTGCCCAGGGCCAGATGACACT
CGATACGTTCATGAGCCGCCAGGCACAGTGGACAGGGCATCTGGTGGAGAAAGGGAAGACGCAGGCTGTGAAGTTTACCG
CCCCACCTTCACCGCCGTGCCCGACATGCAGTGGGTCGATGCGCCGGCGTGCCGGCAAGAAAGGCGGTGGTGCTTTCTGG
GGCTGCAGTAATTATCCCACTTGCCAGGGTGTCATTAACATCGATGCAAAAGGTGGGCGCAAGGCTAAGCGCTATAAAGC
GAAATCACCGACTAAAAATAAAGCAACAGGATCGGAGTAA

Upstream 100 bases:

>100_bases
CACCGGATGCATCGCACCTGACCGATGCTTACCTCGACCGTTTGATGACGCAGTTGCAGCAGGTCGCTAAAGGTCAGCTG
GGCTGACGGGAGGTTATCTG

Downstream 100 bases:

>100_bases
GAGATTGTCTGATGTACATAGCGGAAAGTTTTGCTAAGGTGAGCTAGCTTTGATGCAGGGTACCCCGTAACTGCGGTGGT
GGGAAAATTCTTCCATGGTT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MQLYLCEKPSQGKDIGAILGATQRKQGYLAGAGVIVTWAIGHLLEQAQPETYGEQFGSPWRESVLPIVPTQWKMAVKKDT
ADQFAVIRGLLKQVDSVVIATDADREGEVIARELLEHCHFTGPVQRLWLSALDEASIRHALANKLPGEKTAPLYEAGLAR
SRADWMTGMNLTRLYTLKAREQGYGEVLSIGRVQTPTLALVVNRDRDITNFISKPYWQVRATLQKGGITFPANWVPAANY
CDEEKRCIQQNVAQAVVQRCQQTAHAVVLEVGTERKKESAPLAFDLGTLQQACSRKWGMSANQVLSIAQSLYETHKATTY
PRTDCGYLPTSMREEVADVLSAVTRTDPTLNESVARLDRAFVSRIWNDKKITAHHGIIPTKQPFDLSKLSADELKVYQLI
RQHYLAQYLSLHEVDVTEATFNIGGQLFRTRGKVEAVAGWKVLFQHDVSEAKEEPADTDDVRLPALVKGETVAVAGAELK
TLQTKPPGHFTDGTLIAAMKNAAAFVSDPQLKKVLKENAGLGTEATRAGILDTLFKRGYLEKKGKYLLSTPIAGELIDAL
PTALTNPGMTALWEQALDEIAQGQMTLDTFMSRQAQWTGHLVEKGKTQAVKFTAPPSPPCPTCSGSMRRRAGKKGGGAFW
GCSNYPTCQGVINIDAKGGRKAKRYKAKSPTKNKATGSE

Sequences:

>Translated_679_residues
MQLYLCEKPSQGKDIGAILGATQRKQGYLAGAGVIVTWAIGHLLEQAQPETYGEQFGSPWRESVLPIVPTQWKMAVKKDT
ADQFAVIRGLLKQVDSVVIATDADREGEVIARELLEHCHFTGPVQRLWLSALDEASIRHALANKLPGEKTAPLYEAGLAR
SRADWMTGMNLTRLYTLKAREQGYGEVLSIGRVQTPTLALVVNRDRDITNFISKPYWQVRATLQKGGITFPANWVPAANY
CDEEKRCIQQNVAQAVVQRCQQTAHAVVLEVGTERKKESAPLAFDLGTLQQACSRKWGMSANQVLSIAQSLYETHKATTY
PRTDCGYLPTSMREEVADVLSAVTRTDPTLNESVARLDRAFVSRIWNDKKITAHHGIIPTKQPFDLSKLSADELKVYQLI
RQHYLAQYLSLHEVDVTEATFNIGGQLFRTRGKVEAVAGWKVLFQHDVSEAKEEPADTDDVRLPALVKGETVAVAGAELK
TLQTKPPGHFTDGTLIAAMKNAAAFVSDPQLKKVLKENAGLGTEATRAGILDTLFKRGYLEKKGKYLLSTPIAGELIDAL
PTALTNPGMTALWEQALDEIAQGQMTLDTFMSRQAQWTGHLVEKGKTQAVKFTAPPSPPCPTCSGSMRRRAGKKGGGAFW
GCSNYPTCQGVINIDAKGGRKAKRYKAKSPTKNKATGSE
>Mature_679_residues
MQLYLCEKPSQGKDIGAILGATQRKQGYLAGAGVIVTWAIGHLLEQAQPETYGEQFGSPWRESVLPIVPTQWKMAVKKDT
ADQFAVIRGLLKQVDSVVIATDADREGEVIARELLEHCHFTGPVQRLWLSALDEASIRHALANKLPGEKTAPLYEAGLAR
SRADWMTGMNLTRLYTLKAREQGYGEVLSIGRVQTPTLALVVNRDRDITNFISKPYWQVRATLQKGGITFPANWVPAANY
CDEEKRCIQQNVAQAVVQRCQQTAHAVVLEVGTERKKESAPLAFDLGTLQQACSRKWGMSANQVLSIAQSLYETHKATTY
PRTDCGYLPTSMREEVADVLSAVTRTDPTLNESVARLDRAFVSRIWNDKKITAHHGIIPTKQPFDLSKLSADELKVYQLI
RQHYLAQYLSLHEVDVTEATFNIGGQLFRTRGKVEAVAGWKVLFQHDVSEAKEEPADTDDVRLPALVKGETVAVAGAELK
TLQTKPPGHFTDGTLIAAMKNAAAFVSDPQLKKVLKENAGLGTEATRAGILDTLFKRGYLEKKGKYLLSTPIAGELIDAL
PTALTNPGMTALWEQALDEIAQGQMTLDTFMSRQAQWTGHLVEKGKTQAVKFTAPPSPPCPTCSGSMRRRAGKKGGGAFW
GCSNYPTCQGVINIDAKGGRKAKRYKAKSPTKNKATGSE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=709, Percent_Identity=25.3878702397743, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI4507635, Length=600, Percent_Identity=24.1666666666667, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1788061, Length=621, Percent_Identity=38.8083735909823, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI1787529, Length=599, Percent_Identity=27.3789649415693, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=628, Percent_Identity=25, Blast_Score=129, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=625, Percent_Identity=24, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=678, Percent_Identity=24.3362831858407, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585251, Length=723, Percent_Identity=24.6196403872752, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640096, Length=506, Percent_Identity=25.8893280632411, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 74558; Mature: 74558

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLYLCEKPSQGKDIGAILGATQRKQGYLAGAGVIVTWAIGHLLEQAQPETYGEQFGSPW
CEEEEECCCCCCCCHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCH
RESVLPIVPTQWKMAVKKDTADQFAVIRGLLKQVDSVVIATDADREGEVIARELLEHCHF
HHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCC
TGPVQRLWLSALDEASIRHALANKLPGEKTAPLYEAGLARSRADWMTGMNLTRLYTLKAR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHH
EQGYGEVLSIGRVQTPTLALVVNRDRDITNFISKPYWQVRATLQKGGITFPANWVPAANY
HCCCHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHHCCEECCCCCCCCCCC
CDEEKRCIQQNVAQAVVQRCQQTAHAVVLEVGTERKKESAPLAFDLGTLQQACSRKWGMS
CCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHCCCC
ANQVLSIAQSLYETHKATTYPRTDCGYLPTSMREEVADVLSAVTRTDPTLNESVARLDRA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FVSRIWNDKKITAHHGIIPTKQPFDLSKLSADELKVYQLIRQHYLAQYLSLHEVDVTEAT
HHHHHCCCCEEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FNIGGQLFRTRGKVEAVAGWKVLFQHDVSEAKEEPADTDDVRLPALVKGETVAVAGAELK
HHCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEECCHHH
TLQTKPPGHFTDGTLIAAMKNAAAFVSDPQLKKVLKENAGLGTEATRAGILDTLFKRGYL
EECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
EKKGKYLLSTPIAGELIDALPTALTNPGMTALWEQALDEIAQGQMTLDTFMSRQAQWTGH
HHCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LVEKGKTQAVKFTAPPSPPCPTCSGSMRRRAGKKGGGAFWGCSNYPTCQGVINIDAKGGR
HHHCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
KAKRYKAKSPTKNKATGSE
HHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLYLCEKPSQGKDIGAILGATQRKQGYLAGAGVIVTWAIGHLLEQAQPETYGEQFGSPW
CEEEEECCCCCCCCHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCH
RESVLPIVPTQWKMAVKKDTADQFAVIRGLLKQVDSVVIATDADREGEVIARELLEHCHF
HHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCC
TGPVQRLWLSALDEASIRHALANKLPGEKTAPLYEAGLARSRADWMTGMNLTRLYTLKAR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHH
EQGYGEVLSIGRVQTPTLALVVNRDRDITNFISKPYWQVRATLQKGGITFPANWVPAANY
HCCCHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHHCCEECCCCCCCCCCC
CDEEKRCIQQNVAQAVVQRCQQTAHAVVLEVGTERKKESAPLAFDLGTLQQACSRKWGMS
CCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHCCCC
ANQVLSIAQSLYETHKATTYPRTDCGYLPTSMREEVADVLSAVTRTDPTLNESVARLDRA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FVSRIWNDKKITAHHGIIPTKQPFDLSKLSADELKVYQLIRQHYLAQYLSLHEVDVTEAT
HHHHHCCCCEEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FNIGGQLFRTRGKVEAVAGWKVLFQHDVSEAKEEPADTDDVRLPALVKGETVAVAGAELK
HHCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEECCHHH
TLQTKPPGHFTDGTLIAAMKNAAAFVSDPQLKKVLKENAGLGTEATRAGILDTLFKRGYL
EECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
EKKGKYLLSTPIAGELIDALPTALTNPGMTALWEQALDEIAQGQMTLDTFMSRQAQWTGH
HHCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LVEKGKTQAVKFTAPPSPPCPTCSGSMRRRAGKKGGGAFWGCSNYPTCQGVINIDAKGGR
HHHCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
KAKRYKAKSPTKNKATGSE
HHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]