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The map label for this gene is ubiB

Identifier: 157368499

GI number: 157368499

Start: 280020

End: 281651

Strand: Direct

Name: ubiB

Synonym: Spro_0250

Alternate gene names: 157368499

Gene position: 280020-281651 (Clockwise)

Preceding gene: 157368498

Following gene: 157368500

Centisome position: 5.14

GC content: 54.72

Gene sequence:

>1632_bases
ATGACCCCTGGCGAACTACGCCGCCTGTATTTGATCGTCCGCGTTTTTCTCAGCTATGGCCTGGATGAACTGATCCCCCA
GATGCGCTTGACGTTGCCGCTGCGCGTGGGGCGCCGTCTGCTGTTCTGGATGCCAAACCGCCATGCAGACAAACCGCTGG
GTGAACGTCTGCGTTTGGCCTTGCAGGAACTGGGGCCGGTATGGATCAAGTTTGGCCAGATGATGTCCACACGCCGCGAC
CTGTTCCCACCACAGATCGCCGATCAACTGACTCTGTTGCAGGACCGGGTTGCCCCCTTCGATGGTGCACTGGCACGCAA
GCATATTGAGTTGGCGATGGGCGGCCCGCTGGAAACCTGGTTTGATGATTTTGACCAGCAGGCGCTGGCTTCGGCATCGA
TAGCTCAGGTGCATACCGCACGGCTAAAAACGACCGGGCAGGAAGTGGTTCTGAAGGTGATTCGGCCCGATATTCGGCCG
ATCATCAAGGCTGACGTCCGCCTGATGTATCGTTTGGCGGCCTGGGTGCCAAAGCTGATGCCGGACGGCCGTCGTTTACG
TCCGCGTGAAGTGGTGCGCGAATATGAAAAGACCTTGCTGGACGAGTTGAACCTGCTGCGTGAAGCGGCCAACGCCATCC
AGCTGCGACGCAATTTCGACGGTAGCCCGATGCTGTATGTGCCGGAGGTTTACTCCGATTACTGCCGCGAAAGCGTGTTG
GTCATGGAGCGCATCTACGGAATTCCGGTTTCGGATATTCCAACGCTGGAAAAGCAGGGCACCAACATGAAACTGTTGGC
CGAACGCGGGGTTCAGGTGTTCTTTACCCAGGTGTTCCGCGATAGCTTCTTCCATGCGGACATGCATCCCGGTAATATTT
TTGTCAGCTATGAGCATCCTGAAGATCCCTGCTATATCGGCATTGACTGCGGTATTGTCGGCTCGTTGAACAAAGACGAT
AAACGCTATCTGGCGGAAAACTTTATTGCCTTCTTCAATCGTGACTATCGCAAGGTTGCAGAACTGCACGTTGATTCTGG
CTGGGTACCGCGCGACACCAACGTGGAAGATTTCGAATTCGCTATTCGCACGGTGTGTGAACCCATCTTCGAAAAGCCAC
TGTCGGAAATTTCGTTCGGCAACGTGCTGTTGAACCTGTTCAATACCGCACGCCGTTTCAATATGGAAGTGCAGCCCCAA
CTGGTGTTATTGCAGAAGACCTTGCTGTATGTTGAAGGTCTGGGCCGGCAGCTCTATCCACAGCTGGATTTGTGGACCAC
TGCGAAGCCGTTCCTGGAAAGTTGGATGCGCGATCAGGTCGGTATACCGGCAGTGATCCGCGCACTGAAAGAAAAAGCGC
CGTTTTGGGCCGAGAAACTGCCCGAACTGCCTGAATTATTTTACGATAGCCTGCAGCAGCATAAACTGTTGCAACAAAGC
GTTGATAAGCTGACCAATCAGATGCAGGCCCAGCGCGTTCGCCAGGGACAATCGCGTTATTTGTTCGGCGTTGGCGCTAC
ACTGTTAGTAAGCGGTACGATTTTGCTGTCGGGGGATGTTGAAGTGTTCCCTGCGTGGCTGATAGCGGCCGGTATCGTAT
CCTGGGTGATTGGCTGGAAGCGAACCACCTGA

Upstream 100 bases:

>100_bases
CAGGCCCACTGGAAGTTGTGTGGTTCAACGAGGAAGTTGATGCCACCGTTCGCAACCTCGAAGCGCTGATTGCTCGCATG
GACAAGTTGGAGGGTAAACG

Downstream 100 bases:

>100_bases
ATTGATTCGAAACTCTGGTAAAATCTGCCGTATAATGCGACGGATTTTGAAAAAAACCTGTAAAAATAGAGGTAATTGCA
ATGGGCGGTATTAGTATTAC

Product: putative ubiquinone biosynthesis protein UbiB

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MTPGELRRLYLIVRVFLSYGLDELIPQMRLTLPLRVGRRLLFWMPNRHADKPLGERLRLALQELGPVWIKFGQMMSTRRD
LFPPQIADQLTLLQDRVAPFDGALARKHIELAMGGPLETWFDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRP
IIKADVRLMYRLAAWVPKLMPDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVL
VMERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHPEDPCYIGIDCGIVGSLNKDD
KRYLAENFIAFFNRDYRKVAELHVDSGWVPRDTNVEDFEFAIRTVCEPIFEKPLSEISFGNVLLNLFNTARRFNMEVQPQ
LVLLQKTLLYVEGLGRQLYPQLDLWTTAKPFLESWMRDQVGIPAVIRALKEKAPFWAEKLPELPELFYDSLQQHKLLQQS
VDKLTNQMQAQRVRQGQSRYLFGVGATLLVSGTILLSGDVEVFPAWLIAAGIVSWVIGWKRTT

Sequences:

>Translated_543_residues
MTPGELRRLYLIVRVFLSYGLDELIPQMRLTLPLRVGRRLLFWMPNRHADKPLGERLRLALQELGPVWIKFGQMMSTRRD
LFPPQIADQLTLLQDRVAPFDGALARKHIELAMGGPLETWFDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRP
IIKADVRLMYRLAAWVPKLMPDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVL
VMERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHPEDPCYIGIDCGIVGSLNKDD
KRYLAENFIAFFNRDYRKVAELHVDSGWVPRDTNVEDFEFAIRTVCEPIFEKPLSEISFGNVLLNLFNTARRFNMEVQPQ
LVLLQKTLLYVEGLGRQLYPQLDLWTTAKPFLESWMRDQVGIPAVIRALKEKAPFWAEKLPELPELFYDSLQQHKLLQQS
VDKLTNQMQAQRVRQGQSRYLFGVGATLLVSGTILLSGDVEVFPAWLIAAGIVSWVIGWKRTT
>Mature_542_residues
TPGELRRLYLIVRVFLSYGLDELIPQMRLTLPLRVGRRLLFWMPNRHADKPLGERLRLALQELGPVWIKFGQMMSTRRDL
FPPQIADQLTLLQDRVAPFDGALARKHIELAMGGPLETWFDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRPI
IKADVRLMYRLAAWVPKLMPDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVLV
MERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHPEDPCYIGIDCGIVGSLNKDDK
RYLAENFIAFFNRDYRKVAELHVDSGWVPRDTNVEDFEFAIRTVCEPIFEKPLSEISFGNVLLNLFNTARRFNMEVQPQL
VLLQKTLLYVEGLGRQLYPQLDLWTTAKPFLESWMRDQVGIPAVIRALKEKAPFWAEKLPELPELFYDSLQQHKLLQQSV
DKLTNQMQAQRVRQGQSRYLFGVGATLLVSGTILLSGDVEVFPAWLIAAGIVSWVIGWKRTT

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily

Homologues:

Organism=Homo sapiens, GI41393593, Length=278, Percent_Identity=29.4964028776978, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI40254938, Length=295, Percent_Identity=29.8305084745763, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI32261307, Length=335, Percent_Identity=25.6716417910448, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI217035081, Length=193, Percent_Identity=33.160621761658, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI34147522, Length=214, Percent_Identity=30.8411214953271, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI2367309, Length=545, Percent_Identity=80.1834862385321, Blast_Score=920, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559152, Length=232, Percent_Identity=28.0172413793103, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325148, Length=437, Percent_Identity=23.7986270022883, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323282, Length=249, Percent_Identity=26.1044176706827, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321319, Length=209, Percent_Identity=24.8803827751196, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24662575, Length=245, Percent_Identity=32.6530612244898, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI22024280, Length=263, Percent_Identity=31.5589353612167, Blast_Score=113, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIB_SERP5 (A8G8C0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476488.1
- ProteinModelPortal:   A8G8C0
- STRING:   A8G8C0
- GeneID:   5606593
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0250
- eggNOG:   COG0661
- HOGENOM:   HBG593509
- OMA:   RIQRVVI
- ProtClustDB:   PRK04750
- BioCyc:   SPRO399741:SPRO_0250-MONOMER
- HAMAP:   MF_00414
- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR010232
- TIGRFAMs:   TIGR01982

Pfam domain/function: PF03109 ABC1; SSF56112 Kinase_like

EC number: NA

Molecular weight: Translated: 62605; Mature: 62473

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x21370b0c)-; HASH(0x218aa720)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGELRRLYLIVRVFLSYGLDELIPQMRLTLPLRVGRRLLFWMPNRHADKPLGERLRLA
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHH
LQELGPVWIKFGQMMSTRRDLFPPQIADQLTLLQDRVAPFDGALARKHIELAMGGPLETW
HHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHH
FDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRPIIKADVRLMYRLAAWVPKLM
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
PDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHH
VMERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHP
HHHHHHCCCCCCCCCHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
EDPCYIGIDCGIVGSLNKDDKRYLAENFIAFFNRDYRKVAELHVDSGWVPRDTNVEDFEF
CCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
AIRTVCEPIFEKPLSEISFGNVLLNLFNTARRFNMEVQPQLVLLQKTLLYVEGLGRQLYP
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHCC
QLDLWTTAKPFLESWMRDQVGIPAVIRALKEKAPFWAEKLPELPELFYDSLQQHKLLQQS
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHH
VDKLTNQMQAQRVRQGQSRYLFGVGATLLVSGTILLSGDVEVFPAWLIAAGIVSWVIGWK
HHHHHHHHHHHHHHCCCCCEEEECCHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHCCC
RTT
CCH
>Mature Secondary Structure 
TPGELRRLYLIVRVFLSYGLDELIPQMRLTLPLRVGRRLLFWMPNRHADKPLGERLRLA
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHH
LQELGPVWIKFGQMMSTRRDLFPPQIADQLTLLQDRVAPFDGALARKHIELAMGGPLETW
HHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHH
FDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRPIIKADVRLMYRLAAWVPKLM
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
PDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHH
VMERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHP
HHHHHHCCCCCCCCCHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
EDPCYIGIDCGIVGSLNKDDKRYLAENFIAFFNRDYRKVAELHVDSGWVPRDTNVEDFEF
CCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
AIRTVCEPIFEKPLSEISFGNVLLNLFNTARRFNMEVQPQLVLLQKTLLYVEGLGRQLYP
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHCC
QLDLWTTAKPFLESWMRDQVGIPAVIRALKEKAPFWAEKLPELPELFYDSLQQHKLLQQS
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHH
VDKLTNQMQAQRVRQGQSRYLFGVGATLLVSGTILLSGDVEVFPAWLIAAGIVSWVIGWK
HHHHHHHHHHHHHHCCCCCEEEECCHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHCCC
RTT
CCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA