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The map label for this gene is metE

Identifier: 157368491

GI number: 157368491

Start: 270822

End: 273098

Strand: Direct

Name: metE

Synonym: Spro_0242

Alternate gene names: 157368491

Gene position: 270822-273098 (Clockwise)

Preceding gene: 157368489

Following gene: 157368493

Centisome position: 4.97

GC content: 58.06

Gene sequence:

>2277_bases
ATGGCAATTCTAAATCACACACTGGGTTTTCCTCGCGTTGGGCTACGACGTGAACTGAAAAAAGCTCAGGAAAGCTACTG
GGCAGGTAACTCAACGCAGGAAGAACTGTTGGCAGTTGGCCGCGAGCTGCGCGCTCGCCATTGGCAACAGCAACAACAGG
CCGGGGTGGATTTGGTGCCGGTCGGCGATTTCGCCTGGTACGACCATGTCTTGACCACCAGTCTGCTGCTCGGCAACGTG
CCGGCGCGTCATCAAAATGCGGACGGCACAATCGATCTCGATACGTTGTTCCGCCTGGGCCGTGGCCGCGCACCGACCGG
TGAACCGGCAGCAGCGGCGGAAATGACCAAATGGTTTAACACCAACTACCACTACATGGTGCCCGAATTCCATAAGGGCC
AGCAGTTCAAACTGGGCTGGAGCCAATTGCTGGACGAAGTTGACGAAGCGCTGGCGCTGGGCCACAAAATCAAACCCGTG
CTGTTGGGGCCGGTCACATACCTGTGGCTGGGCAAAGTGAAGGGTGAGCCGTTTGACCGCCTGTCGCTGCTCAACGATAT
CCTGCCGGTTTATCAGCAGGTGCTGGCCGAGCTGGCGAAACGCGGCGTTGAATGGGTGCAGATCGACGAGCCTGCGCTGG
TGCTGGAATTGCCGCAGGCGTGGCTGAACGCCTTCAAACCGGCTTATGACGCGCTGCAGGGCCAGGTAAAACTGCTGCTG
ACTACCTATTTCGACAGCGTTGGCCACAATCTTGACACCCTCCGTGAGTTACCGGTGCAGGGGCTGCACGTTGACCTGGT
TGGCGGTCACGACGATATCGCGGTATTGAATAAGGCGCTGCCGAAAGAGTGGCTGCTGTCACTGGGGGTAATCAACGGTC
GCAACGTCTGGCGCGCCGATCTGAGCAATTGGTTCGAACGTTTGCAGCCATTAGTGGGCAGTCGCCCGCTGTGGATCGGC
ACTTCCTGTTCACTGTTGCACAGCCCGATCGATCTGAGTGTGGAAACCCGGCTGGACGAAGAAGTGAAGAGTTGGTTCGC
CTTCGCCCTGCAAAAATGCGGCGAACTGGCGTTGCTGAGTGCGGCGTTGAACGCACCGGGCGCAGCAAAACAGGCTGAAC
TGGACGCCTACAGCGCGCCAATCCGTGCCCGTCGTCAGTCCAGCCGTGTGCATAATGCGCAGGTCGGTCAACGCCTGGCT
GCTATTACCGCGCAGGACAGCGAACGTCAGCGTCCTTACGCCGAGCGTGCTCAGGCACAGCGTGAACGCTTTAACCTGCC
GGCCTGGCCGACCACTACCATAGGTTCATTCCCGCAAACCACCGAAATTCGCGGCCTGCGTCTGGACTTCAAGCAGGGGC
GTTTGGACGGCAACAACTACCGCACCAGCATTAGCGAGCACATCAAACAGGCGATCGTTGAGCAAGAACGTCTGGGCCTG
GACGTATTGGTACACGGTGAAGCCGAGCGTAACGACATGGTCGAATACTTCGGTGAGAACCTCGACGGCTTCGTTTTCAC
CCAAAACGGCTGGGTACAGAGCTACGGTTCCCGCTGCGTAAAACCGCCGGTGATCATCGGCGATATCAGCCGACCGGAAG
CCATCACCGTAGAGTGGGCGAAGTATGCTCAGTCGCTGACCGACAAGCCGGTGAAGGGCATGTTGACCGGCCCGGTAACC
ATTCTTTGCTGGTCATTCCCGCGTGAAGACGTGACCCGTGAAGTGATTGCCAAACAAATCGCCCTGGCGCTGCGTGATGA
AGTGGAAGATCTGGAAAAAGCCGGCATTGGCATCATCCAGATTGACGAACCGGCACTGCGTGAAGGTCTGCCGCTGCGTC
AATCCGACTGGGCTGCCTACCTGAATTGGGCGGTGGACGCGTTCAAATTGAATGCCGCCGTGGCGAAGGATGATACTCAG
ATCCACACCCACATGTGTTACTGCGAGTTCAACGACATCATGGATTCGATTGCAGCGCTGGATGCTGACGTGATCACCAT
CGAAACCTCGCGTTCCGATATGGATTTGCTGGAAGCCTTTAAAGAGTTTGAATACCCGAACGAAATCGGTCCGGGTGTGT
ATGACATCCACTCGCCAAACGTGCCAAGCGTTGAATGGATCGAAGCGCTGCTGCGTAAAGCCGCGCAGAACATCCCGGCA
GAACGCCTGTGGGTTAACCCGGACTGCGGCCTGAAAACCCGCGGCTGGCCGGAAACCCGTCAGTCGCTGGCCAACATGGT
GCTGGCTGCACAACGCCTGCGCGAGCAGCAGGCCTGA

Upstream 100 bases:

>100_bases
TCCTTCACAGTGACAGTGAATAAATTGCGCTTGTGGTTAATACAGTACCTGCGGATTATGGATGTGTAAACATCTGGACG
GCTAAATGGGGAATATGACG

Downstream 100 bases:

>100_bases
TTGTCGCCGTGGTGCAATGATGTTTTGATCTGACAACCTGGGCGCTTCGGCGCCCTTTTTTATGTCGCAGGCGGCTGTCT
GGTCAGGCAGCCGTGAGGTG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MAILNHTLGFPRVGLRRELKKAQESYWAGNSTQEELLAVGRELRARHWQQQQQAGVDLVPVGDFAWYDHVLTTSLLLGNV
PARHQNADGTIDLDTLFRLGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFHKGQQFKLGWSQLLDEVDEALALGHKIKPV
LLGPVTYLWLGKVKGEPFDRLSLLNDILPVYQQVLAELAKRGVEWVQIDEPALVLELPQAWLNAFKPAYDALQGQVKLLL
TTYFDSVGHNLDTLRELPVQGLHVDLVGGHDDIAVLNKALPKEWLLSLGVINGRNVWRADLSNWFERLQPLVGSRPLWIG
TSCSLLHSPIDLSVETRLDEEVKSWFAFALQKCGELALLSAALNAPGAAKQAELDAYSAPIRARRQSSRVHNAQVGQRLA
AITAQDSERQRPYAERAQAQRERFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGNNYRTSISEHIKQAIVEQERLGL
DVLVHGEAERNDMVEYFGENLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWAKYAQSLTDKPVKGMLTGPVT
ILCWSFPREDVTREVIAKQIALALRDEVEDLEKAGIGIIQIDEPALREGLPLRQSDWAAYLNWAVDAFKLNAAVAKDDTQ
IHTHMCYCEFNDIMDSIAALDADVITIETSRSDMDLLEAFKEFEYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQNIPA
ERLWVNPDCGLKTRGWPETRQSLANMVLAAQRLREQQA

Sequences:

>Translated_758_residues
MAILNHTLGFPRVGLRRELKKAQESYWAGNSTQEELLAVGRELRARHWQQQQQAGVDLVPVGDFAWYDHVLTTSLLLGNV
PARHQNADGTIDLDTLFRLGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFHKGQQFKLGWSQLLDEVDEALALGHKIKPV
LLGPVTYLWLGKVKGEPFDRLSLLNDILPVYQQVLAELAKRGVEWVQIDEPALVLELPQAWLNAFKPAYDALQGQVKLLL
TTYFDSVGHNLDTLRELPVQGLHVDLVGGHDDIAVLNKALPKEWLLSLGVINGRNVWRADLSNWFERLQPLVGSRPLWIG
TSCSLLHSPIDLSVETRLDEEVKSWFAFALQKCGELALLSAALNAPGAAKQAELDAYSAPIRARRQSSRVHNAQVGQRLA
AITAQDSERQRPYAERAQAQRERFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGNNYRTSISEHIKQAIVEQERLGL
DVLVHGEAERNDMVEYFGENLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWAKYAQSLTDKPVKGMLTGPVT
ILCWSFPREDVTREVIAKQIALALRDEVEDLEKAGIGIIQIDEPALREGLPLRQSDWAAYLNWAVDAFKLNAAVAKDDTQ
IHTHMCYCEFNDIMDSIAALDADVITIETSRSDMDLLEAFKEFEYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQNIPA
ERLWVNPDCGLKTRGWPETRQSLANMVLAAQRLREQQA
>Mature_757_residues
AILNHTLGFPRVGLRRELKKAQESYWAGNSTQEELLAVGRELRARHWQQQQQAGVDLVPVGDFAWYDHVLTTSLLLGNVP
ARHQNADGTIDLDTLFRLGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFHKGQQFKLGWSQLLDEVDEALALGHKIKPVL
LGPVTYLWLGKVKGEPFDRLSLLNDILPVYQQVLAELAKRGVEWVQIDEPALVLELPQAWLNAFKPAYDALQGQVKLLLT
TYFDSVGHNLDTLRELPVQGLHVDLVGGHDDIAVLNKALPKEWLLSLGVINGRNVWRADLSNWFERLQPLVGSRPLWIGT
SCSLLHSPIDLSVETRLDEEVKSWFAFALQKCGELALLSAALNAPGAAKQAELDAYSAPIRARRQSSRVHNAQVGQRLAA
ITAQDSERQRPYAERAQAQRERFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGNNYRTSISEHIKQAIVEQERLGLD
VLVHGEAERNDMVEYFGENLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWAKYAQSLTDKPVKGMLTGPVTI
LCWSFPREDVTREVIAKQIALALRDEVEDLEKAGIGIIQIDEPALREGLPLRQSDWAAYLNWAVDAFKLNAAVAKDDTQI
HTHMCYCEFNDIMDSIAALDADVITIETSRSDMDLLEAFKEFEYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQNIPAE
RLWVNPDCGLKTRGWPETRQSLANMVLAAQRLREQQA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=754, Percent_Identity=84.7480106100796, Blast_Score=1325, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=765, Percent_Identity=48.1045751633987, Blast_Score=709, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_SERP5 (A8G8B2)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476480.1
- ProteinModelPortal:   A8G8B2
- SMR:   A8G8B2
- STRING:   A8G8B2
- GeneID:   5606482
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0242
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   SPRO399741:SPRO_0242-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 85121; Mature: 84990

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILNHTLGFPRVGLRRELKKAQESYWAGNSTQEELLAVGRELRARHWQQQQQAGVDLVP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VGDFAWYDHVLTTSLLLGNVPARHQNADGTIDLDTLFRLGRGRAPTGEPAAAAEMTKWFN
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC
TNYHYMVPEFHKGQQFKLGWSQLLDEVDEALALGHKIKPVLLGPVTYLWLGKVKGEPFDR
CCCEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCCCHHH
LSLLNDILPVYQQVLAELAKRGVEWVQIDEPALVLELPQAWLNAFKPAYDALQGQVKLLL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
TTYFDSVGHNLDTLRELPVQGLHVDLVGGHDDIAVLNKALPKEWLLSLGVINGRNVWRAD
HHHHHHHCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCHHHHHHHCCCCCCCHHHHH
LSNWFERLQPLVGSRPLWIGTSCSLLHSPIDLSVETRLDEEVKSWFAFALQKCGELALLS
HHHHHHHHHHHHCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHH
AALNAPGAAKQAELDAYSAPIRARRQSSRVHNAQVGQRLAAITAQDSERQRPYAERAQAQ
HHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHH
RERFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGNNYRTSISEHIKQAIVEQERLGL
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DVLVHGEAERNDMVEYFGENLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWA
EEEEECCCCHHHHHHHHCCCCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCEEEEHHH
KYAQSLTDKPVKGMLTGPVTILCWSFPREDVTREVIAKQIALALRDEVEDLEKAGIGIIQ
HHHHHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IDEPALREGLPLRQSDWAAYLNWAVDAFKLNAAVAKDDTQIHTHMCYCEFNDIMDSIAAL
ECCHHHHCCCCCCCCHHHHHHHHHHHHHEECHHHCCCCCEEEEEEEEEEHHHHHHHHHHC
DADVITIETSRSDMDLLEAFKEFEYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQNIPA
CCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCH
ERLWVNPDCGLKTRGWPETRQSLANMVLAAQRLREQQA
HHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AILNHTLGFPRVGLRRELKKAQESYWAGNSTQEELLAVGRELRARHWQQQQQAGVDLVP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VGDFAWYDHVLTTSLLLGNVPARHQNADGTIDLDTLFRLGRGRAPTGEPAAAAEMTKWFN
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC
TNYHYMVPEFHKGQQFKLGWSQLLDEVDEALALGHKIKPVLLGPVTYLWLGKVKGEPFDR
CCCEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCCCHHH
LSLLNDILPVYQQVLAELAKRGVEWVQIDEPALVLELPQAWLNAFKPAYDALQGQVKLLL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
TTYFDSVGHNLDTLRELPVQGLHVDLVGGHDDIAVLNKALPKEWLLSLGVINGRNVWRAD
HHHHHHHCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCHHHHHHHCCCCCCCHHHHH
LSNWFERLQPLVGSRPLWIGTSCSLLHSPIDLSVETRLDEEVKSWFAFALQKCGELALLS
HHHHHHHHHHHHCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHH
AALNAPGAAKQAELDAYSAPIRARRQSSRVHNAQVGQRLAAITAQDSERQRPYAERAQAQ
HHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHH
RERFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGNNYRTSISEHIKQAIVEQERLGL
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DVLVHGEAERNDMVEYFGENLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWA
EEEEECCCCHHHHHHHHCCCCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCEEEEHHH
KYAQSLTDKPVKGMLTGPVTILCWSFPREDVTREVIAKQIALALRDEVEDLEKAGIGIIQ
HHHHHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IDEPALREGLPLRQSDWAAYLNWAVDAFKLNAAVAKDDTQIHTHMCYCEFNDIMDSIAAL
ECCHHHHCCCCCCCCHHHHHHHHHHHHHEECHHHCCCCCEEEEEEEEEEHHHHHHHHHHC
DADVITIETSRSDMDLLEAFKEFEYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQNIPA
CCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCH
ERLWVNPDCGLKTRGWPETRQSLANMVLAAQRLREQQA
HHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA