| Definition | Escherichia coli HS, complete genome. |
|---|---|
| Accession | NC_009800 |
| Length | 4,643,538 |
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The map label for this gene is nadR [H]
Identifier: 157163837
GI number: 157163837
Start: 4629306
End: 4630538
Strand: Direct
Name: nadR [H]
Synonym: EcHS_A4625
Alternate gene names: 157163837
Gene position: 4629306-4630538 (Clockwise)
Preceding gene: 157163836
Following gene: 157163838
Centisome position: 99.69
GC content: 50.53
Gene sequence:
>1233_bases ATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTAT GACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATTAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTT TTTTGGGGCTTGAATTTCCCCGACAGAAGAAAACCATTGGTGTCGTATTCGGTAAGTTCTACCCGCTGCATACCGGACAT ATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGCGACCGCGC GTTGTTTGAAGACAGCGCCATGTCGCAGCAGCCCACGGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATC AGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGC ATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATAT GGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAA ACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAG TCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTA TGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAAT ACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAA AAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAA CAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGG AGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAG CTGGTGCGGGAGATGATGGGGGAGCAGAGATAA
Upstream 100 bases:
>100_bases CGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAA TGAGAGATACGGAGGGAGAT
Downstream 100 bases:
>100_bases CCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAG ATTAAACAAGGTTTGAAAAA
Product: nicotinamide-nucleotide adenylyltransferase
Products: NA
Alternate protein names: Transcriptional regulator NadR; Nicotinamide mononucleotide adenylyltransferase; NMN adenylyltransferase; NMN-AT; NMNAT; Nicotinamide ribonucleotide adenylyltransferase; Nicotinamide-nucleotide adenylyltransferase; Ribosylnicotinamide kinase; RNK; Nicotinamide riboside kinase; NRK; NmR-K [H]
Number of amino acids: Translated: 410; Mature: 409
Protein sequence:
>410_residues MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGH IYLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRYWEYIPTEVKPFFVRTVAILGGE SSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEIALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCK KYEGREHPFVQALIDEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYDSRFLRCVE LVREMMGEQR
Sequences:
>Translated_410_residues MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGH IYLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRYWEYIPTEVKPFFVRTVAILGGE SSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEIALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCK KYEGREHPFVQALIDEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYDSRFLRCVE LVREMMGEQR >Mature_409_residues SSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHI YLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNGI KKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRYWEYIPTEVKPFFVRTVAILGGES SGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEIALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKK YEGREHPFVQALIDEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYDSRFLRCVEL VREMMGEQR
Specific function: This enzyme has three activities:DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase
COG id: COG3172
COG function: function code H; Predicted ATPase/kinase involved in NAD metabolism
Gene ontology:
Cell location: Cell membrane; Peripheral membrane protein. Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]
Homologues:
Organism=Escherichia coli, GI87082440, Length=410, Percent_Identity=99.5121951219512, Blast_Score=855, Evalue=0.0,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016429 - InterPro: IPR004821 - InterPro: IPR001387 - InterPro: IPR010982 - InterPro: IPR006417 - InterPro: IPR014729 [H]
Pfam domain/function: PF01381 HTH_3 [H]
EC number: =2.7.7.1; =2.7.1.22 [H]
Molecular weight: Translated: 47318; Mature: 47187
Theoretical pI: Translated: 5.34; Mature: 5.34
Prosite motif: PS50943 HTH_CROC1 ; PS00687 ALDEHYDE_DEHYDR_GLU
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.7 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFP CCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC RQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTV CCCCCEEEEEECEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCCCC PDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNGIKKFMAEKGIQPDLIYTSEE HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCC ADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRYWEYIPTEVKPFFVRTVAILGGE CCHHHHHHHCCCCEEEECCHHHHHCCCCCHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCC SSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEIALQYSDYDKIALGHAQYIDFAVK CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHHHH YANKVAFIDTDFVTTQAFCKKYEGREHPFVQALIDEYRFDLVILLENNTPWVADGLRSLG HHCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHC SSVDRKEFQNLLVEMLEENNIEFVRVEEDDYDSRFLRCVELVREMMGEQR CCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCC >Mature Secondary Structure SSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFP CHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC RQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTV CCCCCEEEEEECEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCCCC PDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNGIKKFMAEKGIQPDLIYTSEE HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCC ADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRYWEYIPTEVKPFFVRTVAILGGE CCHHHHHHHCCCCEEEECCHHHHHCCCCCHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCC SSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEIALQYSDYDKIALGHAQYIDFAVK CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHHHH YANKVAFIDTDFVTTQAFCKKYEGREHPFVQALIDEYRFDLVILLENNTPWVADGLRSLG HHCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHC SSVDRKEFQNLLVEMLEENNIEFVRVEEDDYDSRFLRCVELVREMMGEQR CCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 7610040; 9278503; 1327967 [H]