Click here to switch to the map view.

The map label for this gene is yjjW [H]

Identifier: 157163827

GI number: 157163827

Start: 4617691

End: 4618554

Strand: Reverse

Name: yjjW [H]

Synonym: EcHS_A4614

Alternate gene names: 157163827

Gene position: 4618554-4617691 (Counterclockwise)

Preceding gene: 157163828

Following gene: 157163818

Centisome position: 99.46

GC content: 55.56

Gene sequence:

>864_bases
ATGAACAGCAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTT
TTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCG
TGCCACAGTGTCCGCATCAGGCGTTGCAGATTGTTGACGGCAAAGTGGTGTGGAACGCTGTGGTCTGCGAGCAATGTGAT
ACCTGCCTGAAGAGGTGTCCGCAACATGCCACGCCGATGGCGCAATCCATGAGCGTGGACGAAGTGCTTAGCCATGTCCG
CAAAGCGGTGCTGTTTATCGAAGGGATAACGGTGAGTGGCGGCGAAGCCACGACCCAGCTGCCGTTTGTGGTGGCGCTGT
TTACTGCTATCAAAAATGATCCGCAACTGCGCCATCTCACCTGTCTGGTGGACAGTAACGGTATGTTGAGCGAGACCGGC
TGGGAAAAATTGCTCCCGGTGTGCGACGGCGCAATGCTCGATCTCAAAGCATGGGGTAGCGAATGTCATCAACAAATAAC
CGGACGCGATAATCAGCAGATTAAGCGCAGCATCTGTTTGCTGGCAGAGCGCGGTAAGCTGGCGGAACTGCGTTTGCTGG
TAATCCCTGGCCAGGTGGATTATTTGCAACACATCGAAGAACTGGCGGTGTTTATCAAGGGACTTGGCGATGTTCCGGTA
CGCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCCGGAAGACGTTGAGCC
GTTGGCTGATGCATTAAAGGTGCGCGGGGTGAGCCGCTTAATATTCCCGGCGCTGTATTTGTGA

Upstream 100 bases:

>100_bases
AAATATCGCGCCGAAGGTTCACGCACCAACACCACCTGGCTGGGCGAAGAAGCCGCACGCAACACTCGTATTCTGGAACG
CCAGCCGCGCGTGATAAGCC

Downstream 100 bases:

>100_bases
CATGCCGGATACGGCCTACGGTTCGGCACAAACTTGTAGGCATGATAAGACGCGACAAGCGTCGCATCAGGCATTGTGCG
CCAACTGCCGGATGCTGCCT

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCD
TCLKRCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQITGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAVFIKGLGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL

Sequences:

>Translated_287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCD
TCLKRCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQITGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAVFIKGLGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL
>Mature_287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCD
TCLKRCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQITGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAVFIKGLGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790839, Length=287, Percent_Identity=98.9547038327526, Blast_Score=586, Evalue=1e-169,
Organism=Escherichia coli, GI1787130, Length=287, Percent_Identity=26.8292682926829, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 31459; Mature: 31459

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS01087 RADICAL_ACTIVATING ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
5.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQ
CCCCHHHHHHHCCCEECCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCHHH
ALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSG
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEECC
GEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETGWEKLLPVCDGAMLDLKAWGS
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ECHQQITGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAVFIKGLGDVPV
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCE
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL
EEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQ
CCCCHHHHHHHCCCEECCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCHHH
ALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSG
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEECC
GEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETGWEKLLPVCDGAMLDLKAWGS
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ECHQQITGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAVFIKGLGDVPV
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCE
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL
EEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]