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The map label for this gene is bglJ [H]

Identifier: 157163815

GI number: 157163815

Start: 4607475

End: 4608152

Strand: Direct

Name: bglJ [H]

Synonym: EcHS_A4599

Alternate gene names: 157163815

Gene position: 4607475-4608152 (Clockwise)

Preceding gene: 157163814

Following gene: 157163817

Centisome position: 99.22

GC content: 45.28

Gene sequence:

>678_bases
ATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGCAGTATCGGTATTGAGAGTTT
ATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGA
TCTCATTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTG
GCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCC
ATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTG
TACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGACGGAGAGAGAAATA
TTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACA
TAAATTTAATGTGATGTCGAAACTGGGCGTAAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGC
GGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAA

Upstream 100 bases:

>100_bases
GACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCT
GTTTATGGAGATGCCGCAGA

Downstream 100 bases:

>100_bases
TCCGCATGATGCCGGGTTTACTTCCCCGGCAGTGCTTTCATTTCAGCGTACAATCGCCACATTGCTGCACATCCGGTAAG
CGATAACGCTGGCAGCAAGT

Product: DNA-binding transcriptional activator BglJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTEL
AIKFPRTRRLVIADDDIEARLIGSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCETSNVLHPY

Sequences:

>Translated_225_residues
MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTEL
AIKFPRTRRLVIADDDIEARLIGSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCETSNVLHPY
>Mature_225_residues
MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTEL
AIKFPRTRRLVIADDDIEARLIGSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCETSNVLHPY

Specific function: Mutation in bglJ activates the bgl operon [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH luxR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693220, Length=175, Percent_Identity=100, Blast_Score=361, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016032
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE [H]

EC number: NA

Molecular weight: Translated: 25622; Mature: 25622

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQESFQDAMSRISFAAVI
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHH
FSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLIGSLSPSPLDGVLSKASTL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHEECCCCCCCHHHHHHHHHHH
EIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREILRFMSRGYSMTQIAEQLKRN
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
IKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCETSNVLHPY
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQESFQDAMSRISFAAVI
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHH
FSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLIGSLSPSPLDGVLSKASTL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHEECCCCCCCHHHHHHHHHHH
EIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREILRFMSRGYSMTQIAEQLKRN
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
IKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCETSNVLHPY
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8725214; 7610040; 9278503 [H]